• Title/Summary/Keyword: Cell density

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Effect of Mercuric Chloride (In Vitro) on the Function of Human Polylnorphonuclear Leukocytes(PMNs) (수은이 시험관내 사람 다형핵백혈구의 기능에 미치는 영향)

  • 한형미;윤은이;김순한;김옥연;김효정;선우연
    • Biomolecules & Therapeutics
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    • v.1 no.2
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    • pp.131-136
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    • 1993
  • In the present study, the effect of $HgCl_2$on the function of human peripheral polymorphonuclear leukocytes(PMNs) was examined. PMNs were isolated from human peripheral blood with density centrifugation in Ficoll-Paque. The cells were then incubated with $0.5{\sim}5{\mu}M\;HgCl_2$and glass adherence, chemotactic activity and erythrocyte-antibody rosette forming activity were measured. $HgCl_2$ decreased the function of PMNs in all three aspects tested. $HgCl_2$significantly diminished glass adherence(40.5 {\mu}M: 92{\pm}12%$ (percentage of control, $mean{\pm}$ S.D.); 41 {\mu}M: 46{\pm}11%,$ P<0.01; $3{\mu}M: 35{\pm}7%,$P<0.01;$5{\mu}M:49{\pm}10%,$ P<0.01). Similarly, significant differences were observed in chemotactic activity after $HgCl_2$treatment compared with control (control: $0.95{\pm}0.14mm; 0.5 {\mu}M: 0.91{\pm}0.11 mm; 1 {\mu}M: 0.77{\pm}0.16mm, P<0.05; 3{\mu}M: 0.61{\pm} 0.06mm, P<0.01; 5{\mu}M: 0.15{\pm}0.03 mm, P<0.01).$ Also, 4HgCl_2$decreased the percentage of rosette-forming PMNs, indicating diminished phagocytic activity of PMNs upon $HgCl_2$ exposure compared with control (control: $58{\pm}4%; 1{\mu}M: 53{\pm}4%, p<0.05; 3{\mu}M: 49{\pm}3%, P<0.01; 5{\mu}M: 46{\pm}3%, P<0.01).$ Cell viability was not antered after $HgCl_2$treatment (483{\pm}5%$ viability in control PMNs versus $81{\pm}8%$ viability in $5{\mu}M$ Hg-treated PMNs), suggesting that the impaired PMN function after $HgCl_2$treatment was not due to nonspecific cytotoxicity induced by $HgCl_2$. $HgCl_2$-induced decrease in the function of PMNs may have some implications in depressed host susceptibilityupon bacterial challenge after mercury exposure.

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A Study on Anti-oxidative Effect of Electroacupuncture at Kokchi($LI_{11}$) in Rats (곡지(곡지)에 시술(施術)한 저주파 전침자극(電鍼刺戟)의 항산화(抗酸化) 효과(效果)에 대(對)한 실험적(實驗的) 연구(硏究)량)

  • Park, Choon-Eul;Kim, Young-Il;Yim, Yun-Kyoung
    • Journal of Acupuncture Research
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    • v.24 no.3
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    • pp.81-97
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    • 2007
  • Objectives: The purpose of this study is to investigate the anti-oxidative effect of electroacupuncture at Kokchi($LI_{11}$) in rats. Methods: A study administer AAPH to the abdominal cavity of rats and stimulate Kokchi($LI_{11}$) of the rat that lead to oxidative stress by electropuncture. And the study survey serum albumin, total bilirubin, LDL-cholesterol, LDH, Glucose, GOT, GPT and measure SOD activity, GSH concentration, catalase activity, NO concentration, MDA concentration. Results: 1. At the analysis of blood chemistry Albumin and Glucose significantly increase at $LI_{11}$-NR group, $LI_{11}$-EA group than at control group and holder group. LDL cholesterol, GOT, GPT decrease meaningfully. 2. As results of measurement at liver, SOD, Catalase represnet significantly increase at $LI_{11}$-NR group and $LI_{11}$-EA group than at control group and holder group. 3. Glutathione has some increases at the $LI_{11}$-NR group and $LI_{11}$-EA group than at the control group and the holder group. 4. As a result of measurement of NO and MDA's content, the content of NO decrease at the $LI_{11}$-NR group than at the control group. That reduces more meaningfully at the $LI_{11}$-EA group than at the control group. MDA has a significant decrease at the $LI_{11}$-NR group and the $LI_{11}$-EA group. 5. At the histological analysis, the study confirm that the density of intracellular cytoplasm in the liver tissue decreases at the control group as compared with the Normal group and the size of cell increases. Conclusions : These results suppose that electroacupuncture at $LI_{11}$ has an anti-oxidant effect in human.

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The Effect of Synthesis Conditions on the Electrochemical Properties of LiFePO4 for Cathode Material of Secondary Lithium Ion Batteries (리듐 2차 전지용 약극활물질 LiFePO4의 합성 조건에 다른 전기화학적 특성)

  • Kim, Do-Gyun;Park, Hyun-Min;Jeong, Yeon-Uk;Lee, Joon-Hyung;Kim, Jeong-Joo
    • Journal of the Korean Ceramic Society
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    • v.43 no.2 s.285
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    • pp.121-125
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    • 2006
  • [ $LiFePO_4$ ] is one of the promising materials for cathode material of secondary lithium batteries due to its high energy density, low cost, environmental friendliness and safety. $LiFePO_4$ was synthesized by the solid-state reaction method at 500 - 800°C. The crystal structure of $LiFePO_4$ was analyzed by X-ray powder diffraction. The samples synthesized at 600 and $700^{\circ}C$ showed a single phase of a olivine structure. The particle sizes were increased and the specific surface areas were decreased with heating temperatures. The electrochemical performance was investigated by coin cell test. The discharge capacities at 0.1 C-rate were 118 mAh/g and 112 mAh/g at $600^{\circ}C,\;700^{\circ}C$, respectively. In an attempt to improve the electrical conductivity of cathode materials, $LiFePO_4/graphite$ composite was prepared with various graphite contents. The electrical conductivity and discharge capacity were increased with increasing the graphite contents in composite samples. The rate capabilities at high current densities were also improved.

Literature Review of Postoperative Delirium in Geriatric Patients After Elective Gastrointestinal Cancer Surgery

  • Park, Da-In;Choi-Kwon, Smi
    • Journal of Korean Biological Nursing Science
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    • v.20 no.3
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    • pp.177-186
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    • 2018
  • Purpose: Increasing number of older adults are receiving cancer surgeries especially for gastrointestinal cancers, which brings forth attention to age-related postoperative complication prevention. Postoperative delirium (POD) is a common complication that rises after surgical procedures involving general anesthesia, largely in the elderly population. Due to its sudden onset and fluctuating symptoms, POD often goes underdiagnosed and undertreated even though it may lead to various adverse outcomes. POD in GI cancer surgical elderly patients is poorly understood in terms of prevalence, pathophysiology, assessment, treatment and nursing management. We aimed to identify available literature and investigate study results to broaden our understanding of geriatric GI cancer POD. Methods: The search process involved six databases to identify relevant studies abided by inclusion criteria. Results: Eleven studies were selected for this review. Geriatric POD is closely related to frailty and surgical complications. Frailty increases vulnerability to surgical stress and causes cerebral changes that affect stress-regulating neurotransmitter proportions, brain blood flow, vascular density, neuron cell life and intracellular signal transductions. These conditions of frailty result in increased risks of surgical complications such as blood loss, cardiovascular events and inflammation, which all may lead to the POD. Mini Metal State Examination (MMSE), Confusion Assessment Method (CAM) and Delirium Rating Scale-revised-98 (DRS-R-98) are recommended for POD assessment to identify high-risk patients. Conclusion: The POD prevalence ranged from 8.2% to 51.0%. The multifactorial causative mechanism suggests nurses to identify highrisk elderly GI-cancer surgical patients by reviewing patient-specific factors and surgery-specific factors.

Nano Ceramic Coating on Polypropylene Separator for Safety-Enhanced Lithium Secondary Battery (고안전성 리튬이차전지 구현을 위한 나노 세라믹 코팅 분리막 제조 및 전기화학특성 분석)

  • Lee, Jungmo;Jeon, Hyunkyu;Han, Taeyeong;Ryou, Myung-Hyun;Lee, Yong Min
    • Journal of the Korean Electrochemical Society
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    • v.20 no.2
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    • pp.41-48
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    • 2017
  • Herein, we have fabricated an ultrathin aluminum oxide ($Al_2O_3$) coated PP separator by using a RF sputter deposition process. Approximately 20 nm thickness coating layer on the bare PP separator was formed at the power of 55 W for 2 minutes without thermal damage. Whereas only permeability of the coated separator was degraded slightly, other properties such as thermal stability, uptake amount of liquid electrolyte, and ionic conductivity were improved comparing to the bare PP separator. As a result, an only 20-nm-thick $Al_2O_3$ coating layer could improve the rate capability compared with a bare PP separator under a high current density.

The Operating Results of the 75kW MCFC Stack (75kW 용융탄산염 연로전지[MCFC] 스택 운전 결과)

  • Kang, Seung-Won;Kim, Beom-Joo;Kim, Do-Hyeong;Lee, Jung-Hyun;Kim, Eui-Hwan;Lim, Hee-Chun
    • Journal of Hydrogen and New Energy
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    • v.20 no.3
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    • pp.202-207
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    • 2009
  • A 75kW MCFC stack with the reactive area of 9,600cm$^2$ has been operated and validated in Boryeong thermal power plant. The 75kW MCFC stack was installed at the end of November 28, 2008 and started initial operation on December 23, 2008 after pretreated for about 20 days. At initial load operation, the stack showed the Open Circuit Voltage of 137V, which approaches the theoretical value. At the early stage of rated power operation, the stack displayed the voltage of 104V at the current of 754A and reached the maximum generating power of 78.5kW DC. This stack has been operated for 2,890 hours until April, 2009. In addition, the operation time of rated power records 1890 hours. This Operating result is scheduled to be reflected the design of l25kW stack.

Impact Damage of Honeycomb Sandwich Antenna Structures (통신 안테나용 허니콤 샌드위치 구조물의 충격 손상에 관한 연구)

  • Kim, Cha-Gyeom;Lee, Ra-Mi;Park, Hyeon-Cheol;Hwang, Un-Bong;Park, Wi-Sang
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.26 no.2
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    • pp.387-398
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    • 2002
  • The impact response and damage of CLAS panel was investigated experimentally. The facesheet material used was RO4003 woven-glass hydrocarbon/ceramic and the core material was Nomex honeycomb with a cell size of 3.2mm and a density of 96 kg/㎥. The shield plane used was RO4003 and 2024-T3 aluminum. Static indentation and impact test was conducted to characterize the type and extent of the damage observed in two CLAS panels, and the performance of antenna used in a wireless LAN system. Correlation of peak contact force, residual indentation and the delamination area shows impact damage of the panel with an aluminum shield plane is larger than that of the panel with RO4003 shield plane, although the former is more penetration resistant. The damage was observed by naked eye, ultrasonic inspection and cross sectioning. The shape and size of delamination was estimated by ultrasonic inspection, and the area of delamination linearly increases as impact energy increases. The performance of impact damaged antenna was estimated by measuring return loss and radiation pattern. It was revealed that the performance of antenna was related to the impact damage and there was a threshold that the performance of antenna fell as impact energy level changed. The threshold was between the impact energies of 1.5J and 1.75J.

Performance Analysis of Hybrid SOFC/Uncooled GT System for Marine Power Applications (선박동력용 SOFC/GT(무냉각) 하이브리드시스템의 성능 평가)

  • Kim, Myoung-Hwan;Kil, Byung-Lea
    • Journal of Advanced Marine Engineering and Technology
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    • v.36 no.8
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    • pp.1050-1060
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    • 2012
  • As an approach to high-efficiency of SOFC system, SOFC/GT Hybrid system is effective. However, if the output size of the system belongs to the marine class of dozens MWs, the introduction of the cooling system of GT system, which is used as sub-system, makes its related devices complicated and also makes its control difficult. Accordingly, for the marine use, SOFC/GT (non-cooling)Hybrid system looks more suitable than SOFC/GT(cooling)Hybrid system. This study established the SOFC/GT (non-cooling)Hybrid system, and examined the operating temperature & current density of the stack for the system, pressure ratio of the gas turbine, the influence of TIT(Turbine Inlet Temperature) on system performance, etc. through the simulation process. Through this research process, this study was able to confirm that electrical efficiency rises in spite of the increase in the required power for the air compressor, and there exists a limited range of temperatures for operation in TIT.

Two-Dimensional Analysis of Cross-ply Laminates with Transverse Cracks Based on the Assumed Crack Opening Deformation (균열열림변형을 고려한 모재균열이 있는 직교적층판의 2차원 해석)

  • 이재화;홍창선;한영명
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.15 no.6
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    • pp.2002-2014
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    • 1991
  • A refined two-dimensional analysis method, taking into account the crack opening deformation, is proposed for the evaluation of stress distributions in transverse cracked cross-ply laminates. The interlaminar stresses which play an important role in laminate failure are evaluated using the concept of interface layer. A series expansion of the displacements is employed and the thermal residual stresses and Poisson's effects in the laminated are taken into consideration in the formulation. The stress distributions are compared with finite element results. The proposed method represents well the characteristics of the stress distributions. The through-the-thickness variation of the stress distribution is remarkable near the transverse crack due to the crack opening deformation. The interlaminar stresses have significant values at the transverse crack tip and the proposed analysis can be applied as a basis for the prediction of the induced delamination onset by using appropriate failure criteria.

Effect of Electrolyte-Additives on the Performance of Al-Air Cells (전해질 첨가제가 알루미늄-공기전지의 성능에 미치는 영향)

  • Park, Gwun Pil;Chun, Hai Soo
    • Applied Chemistry for Engineering
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    • v.9 no.1
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    • pp.52-57
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    • 1998
  • The effects of additives such as zinc compounds in 4M KOH electrolyte of Al-air cell have been studied. Zinc compounds in electrolyte increased hydrogen evolution overpotential and TPC(tripotasium citrate)/CaO formed fine film on aluminum surface, and these additives decreased hydrogen evolution rate and corrosion rate of aluminum. These additives shifted the OCP in the positive direction on high purity aluminum(purity, 99.999%) and in the negative direction on Al No 1050(purity,99.5%). Addition of two or more additives resulted in the prevention or the reduction of corrosion rate and hydrogen evolution at OCP. As the overpotential on Al electrode increased, the hydrogen evolution rate decreased and the utilization of aluminum increased. At high current density$(>100mA/cm^2)$, TPC/CaO/ZnO additives increased the utilization of high purity aluminum up to that of aluminum alloys containing indium, gallium and thallium.

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