• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.5
• /
• pp.2169-2177
• /
• 2014

Matrine, a main active component extracted from dry roots of Sophora flavecens, has been reported to exert antitumor effects on A549 human non-small lung cancer cells, but its mechanisms of action remain unclear. To determine effects of matrine on proliferation of A549 cells and assess possible mechanisms, MTT assays were employed to detect cytotoxicity, along with o flow cytometric analysis of DNA content of nuclei of cells following staining with propidium iodide to analyze cell cycle distribution. Western blotting was performed to determined expression levels of Bax, Bcl-2, VEGF and HDAC1, while a microarray was used to assessed changes of miRNA profiles. In the MTT assay, matrine suppressed growth of human lung cancer cell A549 in a dose- and timedependent manner at doses of 0.25-2.5 mg/ml for 24h, 48h or 72h. Matrine induced cell cycle arrest in G0/G1 phase and decreased the G2/M phase, while down-regulating the expression of Bcl2 protein, leading to a reduction in the Bcl-2/Bax ratio. In addition, matrine down regulated the expression level of VEGF and HDAC1 of A549 cells. Microarray analysis demonstrated that matrine altered the expression level of miRNAs compared with untreated control A549 cells. In conclusion, matrine could inhibit proliferation of A549 cells, providing useful information for understanding anticancer mechanisms.

• Journal of the Korean Society of Tobacco Science
• /
• v.27 no.2
• /
• pp.153-162
• /
• 2005

Tobacco (Nicotiana tabacum L.) cells were transformed with rice expansin genes, OsEXPA4, OsEXPB3, OsEXPB4, and OsEXPB6, to elucidate the function of the genes in tobacco cells. The transformation increased the mass of the callus by $36\%-65 \%$, and the cell length by $12\%-28\%$. The cell width was decreased by $3\%$ for OsEXPB3, not changed for OsEXPB4, increased by $25\%\;and\;20\%$ for OsEXPA4 and OsEXPB6, respectively. From database search, seven expansin genes were found and six of them belong to EXPA group and one of them belongs to EXPB group. EXLA and EXLB were not found. All tobacco expansin genes were evenly distributed in the phylogenetic tree of rice and Arabidopsis expansin genes.

• Korean Journal of Microbiology
• /
• v.27 no.1
• /
• pp.10-15
• /
• 1989

The plasmid pKOneo, containing SV40 transcriptional promoter, has been used in the mouse tumorigenicity assay for oncogene studies. This assay employs a cotransfection of NIG3T3 fibroblast cells with the desired DNA and the plasmid pKOneo. This oncogene assay, however, has been speculated due to the SV40 transcriptional promoter in the plasmid pKOneo. This research was designed to investigate if the plasmid pKOneo alone is capable of transforming NiH3T3 fibroblast cells. The NIH3T3 subclones were established after the NIH3T3 cells were transfected with the plasmid pKOneo alone. The estabilished NIH3T3 subclones, containing the exogeneous plasmid pKOneo in their chromosomes, were examined for their expression of transformation-associated parameters. The results indicate that this plasmid pKOneo alone has positive effects on transformation of NIH3T3 cells after integration into cellular chromosomes.

• Journal of Preventive Medicine and Public Health
• /
• v.30 no.3 s.58
• /
• pp.555-566
• /
• 1997

This study was carried out to evaluate the cytotoxicity of rock wool fibers(RWFs) such as cell division disturbance, chromosomal and DNA damage, and mutagenicity using cultured cells. RWFs were the man made mineral fibers. In order to find the correlation between the cytotoxicity of RWFs and the phagocytic capacity of cells, the phagocytic processes were observed using scanning electron microscope. Cell division disturbance by RWFs was evaluated by the formation of multinucleated giant cells. The chromosomal damage was evaluated by the micronucleus formation. For the evaluation of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine (8-OH-dG) formation was measured utilizing calf thymus DNA. Mutagenicity was determined by the point mutation of HGPRT and the effect of RWFs on cell transformation was also observed. 1. Compared with the results of chrysotile, RWFs were no or little effect on the cell growth according to the results done by the tests of cell proliferation inhibition and relative plating efficiency. 2. The frequency of multinucleated giant cell formation was increased by the treatment of RWFs and it was dose-dependent. However, the effect of RWFs was weaker than that of chrysotile. 3. The number of micronuclei formed in the RWFs treated cells was between those of cells treated with chrysotile and those of untreated cells. 4. The 2 fold increase in the formation of 8-OH-dG in calf thymus DNA was observed in the cells treated with RWFs in the presence of $H_2O_2$. On the other hand, chrysotile had no effect on the 8-OH-dG formation. 5. RWFs had no effect on the HGPRT point mutation and cell transformation. These results showed that RWFs could induce chromosomal damage, cell division disturbance and oxidative DNA damage in the RWFs treated cells.

• Korean Journal of Crystallography
• /
• v.7 no.2
• /
• pp.196-200
• /
• 1996

The transformation processes from unit cell data, Miller indices and atomic coordinates of non-standard space groups to the ones of the standard space groups in monoclinic system and the calculation processes of their standard deviations have been shown and computerized. The computer programs can be applied to all the seven crystal systems.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.27 no.3
• /
• pp.7-13
• /
• 2004

In this paper, machine cell formation problem is discussed. To reflect precisely actual manufacturing situations such as routing sequences, production quantities, and machining (or operation) characteristics, a new network presentation (or the problem is proposed. It is formulated as a simple 0-1 quadratic programming model with linear constraints. Then, the model is converted into a 0-1 integer programming model using a variable transformation technique. Lastly, some computational results are presented.

• Proceedings of the IEEK Conference
• /
• 2007.07a
• /
• pp.69-70
• /
• 2007

The code division multiple access (CDMA) system capacity is limited by the amount of interference of the system. To reduce the unnecessary interference, this paper proposes optimized cell identification (ID) codes for site selection diversity transmission (SSDT) power control in wideband code division multiple access system of third generation partnership project (3GPP). The proposed SSDT cell ID codes are designed to minimize the problem and to be easily decoded using simple fast Hadamard transformation (FHT) decoder.

• Proceedings of the Korean Society For Composite Materials Conference
• /
• 2002.05a
• /
• pp.57-60
• /
• 2002

The stiffness model has been proposed to predict elastic constants of twisted yarn composites. The model is based upon the unit cell structure, the coordinate transformation, and the volume averaging of compliance constants for constituent materials. For the correlation of analytic results with experiments, composite samples of various yarn twist angle were tested. The samples were fabricated by the RTM process using glass yarns and epoxy resin. The correlations of elastic constants showed relatively good agreements. The model provides the predictions of the three-dimensional engineering constants, which are valuable input data for the analytic characterization of textile composites made of twisted yarn.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.21 no.11
• /
• pp.1757-1764
• /
• 1997

The geometric and elastic models based on the unit cell have been proposed to predict the geometric characteristics and the engineering constants of plain and satin woven composites. In the geometric model, length and inclined angle of the yarn crimp and the fiber volume fraction of woven composites have been predicted. In the elastic model, the coordinate transformation has been utilized to transform the elastic constants of the yarn crimp to those of woven composites, and the effective elastic constants have been determined from the volume averaging of the constituent materials. Good correlations between the model predictions and the experimental results of carbon/epoxy and glass/epoxy woven composites have been observed. Based on the model, the effect of various geometric parameters and materials on the three-dimensional elastic properties of woven composites can be identified.

• Journal of computational fluids engineering
• /
• v.5 no.1
• /
• pp.14-21
• /
• 2000

A method of two and three dimensional orthogonal grid generation with control of spacing by using the covariant Laplace equation is presented. An important feature of the methodology is its ability to control effectively the grid spacing especially near the boundaries still maintaining good orthogonality in whole field. The method is based on the concept of decomposition of the global transformation into consecutive transformation of an approximate conformal mapping and an auxiliary orthogonal mapping to have linear and uncoupled equations. Control of cell spacing is based on the concept of reference arc length, and orthogonal correction is peformed in the auxiliary domain. It is concluded that the methodology can successfully generate well controlled orthogonal grids around bodies of 2 and 3 dimensional configurations.