• Preventive Nutrition and Food Science
• /
• 제16권3호
• /
• pp.217-223
• /
• 2011

Ischemic stroke constitutes about 80% of all stroke incidences. It is characterized by brain cell death in a region where cerebral arteries supplying blood are occluded. Under these ischemic conditions, apoptosis is responsible for the cell death, at least in part. Goat's-beard (Aruncus dioicus var. kamtschaticus) is a perennial plant that grows naturally in the alpine regions of Korea. In the present study, we first determined whether water extract of goat's-beard (HY1646) and some of its fractions prepared by partitioning with organic solvents could improve the viability of human hepatocellular carcinoma cells (HepG2) cultured under hypoxic condition by blocking apoptotic pathways. Based on the in vitro findings, we subsequently investigated whether HY1646 and the ethyl acetate fraction (EA) selected from cell culture-based screening could attenuate brain injury in a rat middle cerebral artery occlusion (MCAO) model of ischemia (2 hr), followed by 22 hours of reperfusion. The cell number was sustained close to that initially plated in the presence of HY1646 even after 24 hr of cell culture under hypoxic condition (3% $O_2$), at which time the cell number reached almost zero in the absence of HY1646. This improvement in cell viability was attributed to the delay in apoptosis, identified by the formation of DNA ladder in gel electrophoresis. Of fractions soluble in hexane, ethyl acetate (EA) and butanol, EA was chosen for the animal experiments because EA demonstrated the best cell viability at the lowest concentration (10 ${\mu}g$/mL). HY1646 (200 mg/kg) and EA (10 and 20 mg/kg) significantly reduced infarct size, an index of brain injury, by 16.6, 40.0 and 61.0%, respectively, as assessed by 2,3,5-triphenyl tetrazolium chloride staining. The findings suggest that prophylactic intake of goat's beard might be beneficial for preventing ischemic stroke.

• 한국생명과학회:학술대회논문집
• /
• 한국생명과학회 2002년도 제38회 학술심포지움
• /
• pp.48-60
• /
• 2002

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the production of granulocytes, macrophages, and white blood cells. The effects of osmotic pressure on secretion of human GM-CSF into the culture medium were investigated in suspension cultures of transgenic tobacco cells. An increase in osmotic pressure caused by the addition of mannitol decreased the cell size index, with the effect being more pronounced when cells were measured wet rather than dry. Increased osmotic pressure enhanced the secretion of hGM-CSF. At 90 g/L mannitol, the maximum concentration tested, hGM-CSF was present in the culture medium at 980 ug/L. As the concentration of mannitol increased, the total amount of protein secreted also increased, but was disproportionately enriched in GM-CSF NaCl, another osmoticum, had very similar effects on cell growth and hGM-CSF production, but did not cause enrichment for hGM-CSF Additionally, protein-stabilizing polymer was added to culture broth to enhance stability of secreted recombinant protein. Finally, above two method were applied together to maximize the productivity.

• 한국습지학회지
• /
• 제13권1호
• /
• pp.105-116
• /
• 2011

최근 몇 년간 기후변화에 의해 기상이변이 발생하고 있으며 이에 따른 집중호우로 인한 홍수피해가 심각하게 증가하고 있다. 이러한 피해를 저감하기 위한 수문기상학적 요소와 특성인자들의 정확한 상호 연관성 규명과 공간적 변동성 해석은 강우-유출 모형에서 발생하는 불확실성을 감소시키는데 중요한 요소로 작용하게 된다. 이에 본 연구에서는 레이더강우 격자 해상도와 지형인자 격자 해상도에 따라 강우-유출모형이 어떻게 반응하는지 분석하였으며, 가-분포 강우-유출 모형인 ModClark 모형을 이용하여 강원도 인제군의 내린천 유역을 대상으로 광덕산 레이더자료를 이용하였다. ModClark 모형 구성을 위한 GIS 지형공간 자료는 30m, 150m, 250m, 350m 격자크기의 DEM을 사용하였으며, 2006년 7월 14일부터 7월 17일까지의 관측레이더 강우자료를 500m, 1km, 2km, 5km, 10km 사용하여 유출모의를 실시하고, 각각의 격자해상도에 따른 모의 결과를 비교하기 위해 유출 수문곡선을 작성하고 유출량 변화를 모의하였다. 분석 결과 첨두유량 및 유출체적에 대해서는 DEM 30m~150m, 레이더강우 500m~2km 크기의 격자일 때 가장 최적의 유출 모의를 한 것으로 분석되었으며, 통계적 분석에 의한 분석결과에서는 모든 DEM 격자는 레이더강우 격자가 500m인 경우, 모든 레이더강우 격자는 DEM 30m인 경우에 모형의 적합성이 높은 것으로 나타났고, 민감도 산정 결과 지수 등급이 높은 DEM이 분포형 모형의 결과 값에 큰 영향을 주는 것으로 분석되었다. 최근 집중형 모형에서 분포형 모형을 이용한 강우-유출해석이 이루어지고 있기에 모델링 구성을 위한 효율적인 의사결정의 기준으로 활용될 수 있을 것으로 기대된다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권3호
• /
• pp.939-944
• /
• 2016

Background: Cell-free DNA circulating in blood is a candidate biomarker for malignant tumors. Unlike uniformly truncated DNA released from apoptotic non diseased cells, DNA released from necrotic cancer cells varies in size. Objectives: To measure the DNA integrity index in serum and the absolute DNA concentration to assess their clinical utility as potential serum biomarkers for colorectal carcinoma (CRC) compared to CEA and CA19-9. Materials and Methods: Fifty patients with CRC, 10 with benign colonic polyps and 20 healthy sex and age matched volunteers, were investigated by real time PCR of ALU repeats (ALU q-PCR) using two sets of primers (115 and 247 bp) amplifying different lengths of DNA fragments. The DNA integrity index was calculated as the ratio of q-PCR results of ALU 247/ALU 115bp. Results: Serum DNA integrity was statistically significantly higher in CRC patients compared to the benign and control groups (p<0.001). ROC curves for differentiating CRC patients from normal controls and benign groups had areas under curves of 0.90 and 0.85 respectively. Conclusions: The DNA integrity index is superior to the absolute DNA concentration as a potential serum biomarker for screening and diagnosis of CRC. It may also serve as an indicator for monitoring the progression of CRC patients. Combining CEA and CA19-9 with either of the genetic markers studied is better than either of them alone.

• Korean Journal of Acupuncture
• /
• 제24권2호
• /
• pp.163-184
• /
• 2007

Objectives & Methods : The purpose of this study is to observe the effects of Phellodendri Cortex Herbal-acupuncture solution (PC-HAS) at Joksamni (ST36) on collagen II induced arthritis in DBA-1J mice. The author performed several experimental items to analyze arthritis evaluation, change of weight, spleen size and adhesion rate, change of cytokine level, IgG, IgM and anti-collagen II, chang of immunocyte count, histological change of CIA mouse joint. Results : 1. In the PC-HA group, arthritis index, the incidence of arthritis and joint edema were significantly decreased. 2. In the PC-HA group, the change of spleen size, spleen adhesion rate and the knee joint were significantly decreased. 3. The levels of $IL-1{\beta}$, IL-6 and INF- in serum of the CIA mouse were significantly decreased by PC-HA. 4. The levels of IgG, IgM and anti-collagen II in serum of the CIA mouse were significantly decreased by PC-HA. 5. In the CIA mouse spleen cell culture, the levels of IFN- , IFN- / IL-4, IL-10 were significantly decreased by PC-HA, but the level of IL-4 was significantly increased by PC-HA. 6. In the PC-HA group, the ratios of $CD3e^+$ to $CD45R^+$ cell, $CD4^+$ to $CD8^+$ cell and $CD4^+/CD25^+$ cell were similarly maintained as normal group in the CIA mouse spleen cell. 7. In the PC-HA group, $CD4^+CD25^+$ and $CD45R^+/CD69^+$ cell were significantly decreased in the lymph nodes. 8. In the PC-HA group, $CD3^+/CD69^+$ and $CD11b^+/Gr-1^+$ cell were significantly decreased in knee joint. 9. In histology, the cartilage destruction and synovial cell proliferation in the PC-HA group were similar with that of the normal group and the collagen fiber expressions in the PC-HA group were similar with that of the normal group. Conclusions : Form the result above, the results suggest that the PC-HA at ST36 has significant effect on collagen-induced arthritis, and can be put to practical use in the future rheumatoid arthritis clinic.

• 한국식품과학회지
• /
• 제20권4호
• /
• pp.463-469
• /
• 1988

쌀가루의 압출성형 특성을 조사하기 위하여 원료의 수분합량과 입자크기를 달리하여 자가발열형 single screw extruder로 처리하고 이때 온도변화에 따른 extrudate의 물성학적인 성질을 비교하였다. 원료의 수분함량은 17%, 19%, 22%, 25%, 28%로 했고 입자크기는 18-60mesh와 60-120mesh의 두 범위로 했다. 내부흐름의 온도는 자동 온도기록계에 기록되게 하여 정상상태 가동이 될 때까지 경시적으로 시료를 채취하였다. 팽화율은 수분함량과 입자크기가 감소함에 따라 증가하였으며, bulk density는 팽화율과 역상관 관계를 나타내었다. Extrudate의 절단력은 수분함량이 적고 입자조기가 감소함에 따라 낮아졌으며 기공의 크기도 균일하였다. 색도는 수분함량과 입자크기가 증가함에 따라 명도는 증가하였고 황색도는 감소하였다. 겉보기 점도는 수분함량이 증가함에 따라 증가했고, 수용성 지수는 감소하였다. 원료의 수분이 감소함에 따라 일부의 전분은 호정화를 유발하는 것으로 판단되었다.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2010년도 제39회 하계학술대회 초록집
• /
• pp.281-281
• /
• 2010

Very low refractive index (<1.4) materials have been proved to be the key factor improving the performance of various optical components, such as reflectors, filters, photonic crystals, LEDs, and solar cell. Highly porous SiO2 are logically designed for ultralow refractive index materials because of the direct relation between porosity and index of refraction. Among them, ordered macroporous SiO2 is of potential material since their theoretically low refractive index ~1.10. However, in the conventional synthesis of ordered macroporous SiO2, the time required for the crystallization of organic nanoparticles, such as polystyrene (PS), from colloidal solution into well ordered template is typical long (several days for 1 cm substrate) due to the low interaction between particles and particle - substrate. In this study, polystyrene - polyacrylic acid (PS-AA) nanoparticles synthesized by miniemulsion polymerization method have hydrophilic polyacrylic acid tails on the surface of particles which increase the interaction between particle and with substrate giving rise to the formation of PS-AA film by simply spin - coating method. Less ordered with controlled thickness films of PS-AA on silicon wafer were successfully fabricated by changing the spinning speed or concentration of colloidal solution, as confirmed by FE-SEM. Based on these template films, a series of macroporous SiO2 films whose thicknesses varied from 300nm to ~1000nm were fabricated either by conventional sol - gel infiltration or gas phase deposition followed by thermal removal of organic template. Formations of SiO2 films consist of interconnected air balls with size ~100 nm were confirmed by FE-SEM and TEM. These highly porous SiO2 show very low refractive indices (<1.18) over a wide range of wavelength (from 200 to 1000nm) as shown by SE measurement. Refraction indices of SiO2 films at 633nm reported here are of ~1.10 which, to our best knowledge, are among the lowest values having been announced.

• 한국식품과학회지
• /
• 제21권6호
• /
• pp.772-779
• /
• 1989

분리대두단백의 조직화에 대한 스크류 회전속도와 원료 수분함량의 영향을 조사하였다. 수분함량이 증가함에 따라 토출구온도는 감소하였으며 210rpm에서 가장 높은 온도를 나타내었다. 수용성 질소지수는 수분함량이 증가할수록 감소하였으며 고속보다는 저속에서 감소폭이 컸다. 조직잔사지수는 낮은 수분함량과 고속 rpm에서 높게 나타났으며 조직감의 강도는 이와 반대의 경향이었다. 또한 원료의 수분함량이 증가할수록 최종 압출성형물의 수분함량이 증가하였고 고속 rpm일수록 낮은 수분함량을 나타내었다. 밀도의 경향도 이와 일치하였다. 재흡수력의 경우는 수분함량 30%에서 최고치를 나타내었으며 고속 rpm에서 증가하였다. 복원전의 명초는 복원 후 보다 높은 수치를 보였고 고속회전일 수록 명도가 밝아졌다. 성상에 있어서도 저수분, 고속 rpm에서 높은 명도와 높은 정화도가 관찰되었다. 분산분석을 통해서 스크류 회전속도보다 원료의 수분함량이 조직화 성질을 나타내는 각 지표에 더 깊은 영향을 주는 것으로 확인되었다.

• 한국식품과학회지
• /
• 제35권5호
• /
• pp.871-876
• /
• 2003

본 연구는 뽕잎분말을 케이크의 제조에 기능성 소재로 이용하고자 실시되었으며, 이를 위하여 밀가루의 4, 8, 12, 16, 20%를 뽕잎분말로 대체하여 yellow layer cake를 제조하였다. 케이크의 품질은 반죽의 점도와 비중, 케이크의 baking 특성 및 저장기간중의 텍스쳐 변화를 측정하여 평가하였다. 뽕잎분말의 첨가량이 증가할수록 반죽의 점도와 케이크의 비체적은 증가하였다. 케이크의 대칭지수는 뽕잎분말의 양이 증가함에 따라서 감소하였으나, 총부피지수나 균일성 지수는 변화가 없었다. 저장기간 중의 경도변화는 뽕잎분말의 첨가량이 증가할수록 경도증가율이 낮았으며, 대조구보다 덜 단단한 것으로 나타났다. 관능검사결과 뽕잎분말을 첨가한 케이크의 부드러움성은 차이가 없었으며 촉촉한 정도는 뽕잎분말을 첨가한 케이크가 더 크게 나타났다.

• Applied Microscopy
• /
• 제28권4호
• /
• pp.447-463
• /
• 1998

This study was carried out to investigate the histomorphological changes and the electrophoretic patterns of egg components, obtained from 100 of 2-year-old female catfishes (Silurus asotus). Female Korean catfishes collected in the vicinity of Chollabukdo have synchronous ovaries which discontinously ovulate eggs during the breeding season (from late May until early July). The fishes were killed by severing the spinal cord just posterior to the head after immobilization with tricaine methanesulfonate (MS 222). Especially, the light microscopic and ultrastructural changes of ooplasm and follicular membranes of oocytes, were examined by means of light and transmission electron microscopy. The size of the nucleoli and number of the yolk granules increased as the oocyte groved. Yolk granules were deposited in the oocyte as fluid Due to tile presence of large early and late maturing oocytes, their ovaries were enlarged, transparent, granular and greenish in color. As the percentages of fish in late maturing oocyte (LMO) and ripe oocyte (RO) stage increased from March to April, mean gonadosomatic index (GSI) values (19.95%) increased. Zona radiata changed from squamous into cuboid in shape in the early maturing oocyte (EMO) stage. Processes, microvilli, from the zona radiata and from the oocyte grow, and make contact with each other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radials becomes more compact and devoid of pore canals during oocyte maturation. Seasonal changes in the microscopic appearance of the ovaries were well correlated with those in both gonadosomatic index and macroscopic appearance. The main cytological changes such as increase in size of cell, nucleus, nucleolus, and increase in number of nucleoli and mitochondria demonstrated with electron microscopy in the previtellogenic oocytes of Korean catfish, provided evidences for important synthetic processes in an early preparatory phase of oocyte development. The electrophoretic pattern of major band in mature stage was much thicker (24 k, 66 k, 90-110k dalton) than that in previtellogenic phase.