• Korean Journal of Veterinary Research
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• v.37 no.2
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• pp.375-381
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• 1997

The purpose of the production of monoclonal antibodies aganist the Canine distemper virus(CDV) were perfect diagnosis and a new approach to treat canine distemper because the diagnosis and treatment of canine distemper were difficult. Canine distemper virus(CDV) was purified using saturated ammonium sulfate, and injected into hind footpads of BALB/c mouse. 12-15 days later, popliteal lymph node(PN) cells were harvested and fused with SP2/O myeloma cells. Characteristics of monoclonal antibodies were analysed. 1. 9 hybridomas produce the specific antibody against CDV. 2. 6 monoclonal antibodies are against intranuclear and cytoplasmic component of CDV, and 3 monoclonal antibodies are against cytoplasmic inclusions. 3. All monoclonal antibodies did not react with other 5 different viruses (CAV-I, CAV-II, CCV, CPV and CPIV) and react with another CDV-FXNO strain. 4. 3 monoclonal antibodies have neutralizing activity against CDV. 5. Antigenic difference was observed between CDV by IFA.

• Korean Journal of Veterinary Research
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• v.35 no.1
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• pp.75-85
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• 1995

An indirect immunofluorescent antibody test was applied to survey the antibody prevalence on five canine viruses including canine distempervirus(CDV), canine parvovirus(CPV), canine coronavirus(CCV), canine adenovirus type-2(CAV-2), canine parainfluenzavirus(CPIV) in dogs. The period studied was from October 1992 to June 1993. A total of 80 dog sera was collected from veterinary clinics in Kwangju and Seoul, and examined for the presence of virus antibodies. Immunofluorescent antibodies(IFA) to all viruses were present in a high percentage of 80 sera tested. Seventyfive(93.8%) showed detectable IFA against CPV, 67(83.8%) against CDV, 51(63.8%) against CCV, 42(52.5%) against CPIV and 34(42.5%) against CAV-2. These suggested that all viruses were endemic in the communities. IFA levels against each virus were also distributed fairly irregularly. IFAs for CDV and CPV were detected more frequently with a relatively high incidence in vaccinated group less than 1 years of age. IFAs for CAV-2 were detected more frequently with growing age. In the correlation of clinical signs and antibody prevalence, dogs that showed hematochezia and vomiting had high titers in the positive sera is noteworthy, particularly for CDV and CPV. The significance between dogs those who had diarrhea, dyspnea and salivation and those viruses were obscure.

• Journal of Auto-vehicle Safety Association
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• v.14 no.1
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• pp.6-13
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• 2022

The Ministry of Land, Infrastructure and Transport (MoLIT) and the Korean Automobile Testing and Research Institute (KATRI) are supporting the development of Lv.3 automated vehicle (hereinafter, AV) technology by constructing an automated driving pilot city (as known as K-City) equipped with total 5 evaluation environments (urban, motorway, suburban, community road, and autonomous parking facility) which is a test bed exclusively for AV (2017~2018). An upgrade project is in a progress to materialize harsh environments such as bad weather (rain, fog, etc.) and reproduction of communication jamming (GPS blocking, etc.) with the purpose of supporting the development of Lv.4 connected & automated vehicle (hereinafter, CAV) technology (2019~2022). We intend to proactively establish a national level standard for CAV test-bed and test road requirements, test method, etc. for establishment of a road map for the construction of the test bed which is being promoted step by step and analyze and, when required, benchmark the case of China that has announced and is utilizing it. Through this, we plan to define standardized requirements (evaluation facility, evaluation system, etc.) on the test bed for the development of Lv.4/4+ CAV technology and utilize the same for the design and construction of a test bed, establishment of a road map for the construction of a real car-based test environment related to the support for autonomous driving service substantiation, etc. through provision of an evaluation environment utilizing K-City, and the establishment of a K-City upgrade strategies, etc.

• Genomics & Informatics
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• v.19 no.2
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• pp.14.1-14.6
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• 2021

Coronavirus disease 2019 (COVID-19) is an on-going pandemic disease infecting millions of people across the globe. Recent reports of reduction in antibody levels and the re-emergence of the disease in recovered patients necessitated the understanding of the pandemic at the core level. The cases of multiple organ failures emphasized the consideration of different organ systems while managing the disease. The present study employed RNA sequencing data to determine the disease associated differentially regulated genes and their related protein interactions in several organ systems. It signified the importance of early diagnosis and treatment of the disease. A map of protein interactions of multiple organ systems was built and uncovered CAV1 and CTNNB1 as the top degree nodes. A core interactions sub-network was analyzed to identify different modules of functional significance. AR, CTNNB1, CAV1, and PIK3R1 proteins were unfolded as bridging nodes interconnecting different modules for the information flow across several pathways. The present study also highlighted some of the druggable targets to analyze in drug re-purposing strategies against the COVID-19 pandemic. Therefore, the protein interactions map and the modular interactions of the differentially regulated genes in the multiple organ systems would incline the scientists and researchers to investigate in novel therapeutics for the COVID-19 pandemic expeditiously.

• Proceedings of the IEEK Conference
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• 2002.07b
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• pp.1296-1299
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• 2002

We implemented a CD signal processor operated on a CAV 48-speed CD-ROM drive into a VLSI. The CD signal processor is a mixed mode monolithic IC including servo-processor, data recovery, data-processor, and I-bit DAC. For servo signal processing, we included a DSP core, while, for CAV mode playback, we adopted a PLL with a wide recovery range. Data processor (DP) was designed to meet the yellow book specification.［2］So, the DP block consists of EFM demodulator, C1/C2 ECC block, audio processor and a block transferring data to an ATAPI chip. A modified Euclid's algorithm was used as a key equation solver for the ECC block To achieve the high-speed decoding, the RS decoder is operated by a pipelined method. Audio playability is increased by playing a CD-DA disc at the speed of 12X or 16X. For this, subcode sync and data are processed in the same way as main data processing. The overall performance of IC is verified by measuring a transfer rate from the innermost area of disc to the outermost area. At 48-speed, the operating frequency is 210 ㎒, and this chip is fabricated by 0.35 um STD90 cell library of Samsung Electronics.

• KIEAE Journal
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• v.16 no.2
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• pp.23-31
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• 2016

Purpose: Recently energy consumption is rapidly increasing due to continuous development of social evolution in various field. In this situation, there is a lot of effort to reduce this energy consumption in many ways, especially in building energy. Preceding studies already started to analyze the housing area such as zero energy house and passive house by researching annual building energy consumption, but to apply the results of housing to office building is insufficient since it has different consumption tendency. Method: In this study, eQuest program was used for simulation and the base model is selected among standard office building in ASHRAE 90.1. Variables are divided into passive and active factors for comparison. Result: In passive factors, glazing system showed the highest energy saving rate by 21.3% with triple low-e glass and enhancing wall u-value showed the lowest energy saving rate by 3.6% with 0.15 m2/K. In active factors, VAV system showed 30.9% energy saving rate when compared to CAV system, and heat exchanger showed 10.2% energy saving rate. For regeneration energy part, photovoltaic panel generated 10.4% of base annual energy usage.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.9
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• pp.1507-1515
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• 2018

Objective: In the poultry industry, the most important economic traits are meat quality and carcass yield. Thus, many studies were conducted to investigate the regulatory pathways during muscle differentiation. To gain insight of muscle differentiation mechanism during growth period, we identified and validated calcium-related genes which were highly expressed during muscle differentiation through mRNA sequencing analysis. Methods: We conducted next-generation-sequencing (NGS) analysis of mRNA from undifferentiated QM7 cells and differentiated QM7 cells (day 1 to day 3 of differentiation periods). Subsequently, we obtained calcium related genes related to muscle differentiation process and examined the expression patterns by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Results: Through RNA sequencing analysis, we found that the transcription levels of six genes (troponin C1, slow skeletal and cardiac type [TNNC1], myosin light chain 1 [MYL1], MYL3, phospholamban [PLN], caveolin 3 [CAV3], and calsequestrin 2 [CASQ2]) particularly related to calcium regulation were gradually increased according to days of myotube differentiation. Subsequently, we validated the expression patterns of calcium-related genes in quail myoblasts. These results indicated that TNNC1, MYL1, MYL3, PLN, CAV3, CASQ2 responded to differentiation and growth performance in quail muscle. Conclusion: These results indicated that calcium regulation might play a critical role in muscle differentiation. Thus, these findings suggest that further studies would be warranted to investigate the role of calcium ion in muscle differentiation and could provide a useful biomarker for muscle differentiation and growth.

• Radiation Oncology Journal
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• v.11 no.1
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• pp.97-102
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• 1993

A retrospective analysis of various characteristics in 32 limited stage small cell lung cancer patients treated at the Department of Therapeutic Radiology in Kangnam St. Mary's Hospital, Catholic University Medical College from April 1983 to September 1991, was carried out to identify factors which had prognostic significance for survival from initiation of radiation therapy. There were 26 men and 6 women. Median age was 63 years (range: 24-78 years). The follow up duration was 1.5 to 44 months (median: 9 months). External radiation therapy was done with daily 160-180 cGy,5 fraction/week, total of 1000-6660 cGy (median: 4500 cGy) to the mediastinum by 6 MV linear accelerator. Of 32 Patients, 27 ($84.4{\%}$) patients were treated with combined modality (chemotherapy plus radiation therapy), and 5 ($15.6{\%}$) patients were treated with radiation therapy only. Complete responders were 12 patients ($37.5{\%}$), partial responders were 11 ($34.4{\%}$), and no responders were 9($28.1{\%}$). Karnofsky performance status over 70 (p<0.04), chemotherapy regimen (CAV, PV, and CAV+PV) (p<0.04),6 or more cycles of chemotherapy (p<0.007), radiation therapy over 4500 cGy (p<0.03), and radiation therapy responder (CR+PR) (p<0.003) showed a significantly favorable influence on 1 year survival rate. Age (p=0.545), sex (p=0.666), presence of superior vena cava syndrome (p=0.719), prophylactic cranial irradiation (p=0.217), and radiation therapy duration (p=0.491) had no effect on survival. Radiation induced side effects were transient esophagitis in 11 ($34{\%}$), general weakness in 9($28{\%}$), gastrointestinal symptoms in terms of nausea, vomiting and indigestion in 5 ($15{\%}$) and leukopenia in 1 ($3{\%}$).

• Korean Journal Plant Pathology
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• v.3 no.2
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• pp.124-130
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• 1987

The optimum conditions for protoplast formation and regeneration from Pyricularia oryzae Cav. were selected as follows. As a basic solution, 0.02M potassium phosphate buffer solution plus 0.6M KCl adjusted to pH 5.2 with 1N HCl was used. A mixture of enzyme combinations with 20mg Cellulase R-l0/ml, 5mg Macerozyme R-l0/ml and l0mg Driselase/ml used as a lytic enzyme showed better lytie effect than any single enzyme treatment for protoplast formation. Two-day-old mycelia of P. oryzae grown in the mixture of three lytie enzyme solution at $30^{\circ}C$ for 3 hr showed best condition for protoplasts formation. For regeneration from the protoplasts of P. oryzae, potato dextrose agar containing 0.02M potassium phosphate plus 0.6M KCl used as a stabilizer was best for regeneration medium.

• Proceedings of the Korean Nuclear Society Conference
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• 1997.05a
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• pp.569-574
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• 1997

프랑스 가압경수형 원전인 울진 1,2호기 cavity의 격납용기 직접가열에 대한 특성을 분석하기 위하여 노심 용융물 고압분출 실험 연구를 수행하였다. 울진 1,2호기 격납건물은 국내의 Westinghouse와 CE형 원전과 달리 원자로용기 하부 cavity내 수평통로에 의한 출구가 없어 원자로용기 외곽의 환형통로를 통해 cavity와 격납건물 상부 대기와 직접 연결되어 있는 특성을 가지고 있다. 본 연구에서는 환형통로 면적, 파손 직경 용융물 질량 등에 따라 원자로용기 압력을 변화시키면서 용융물을 물, 증기를 질소기체로 각각 모의하여 실험을 수행하였다. 실험결과, 노심용융물 고압분출에는 원자로용기 파손 직경이 많은 영향을 미치는 것으로 나타났고 환형통로 면적과 용융물 질량은 큰영향을 미치지 않는 것으로 나타났다. 또한 환형통로 면적의 감소는 노심용융물 고압분출시 cav의 압력을 다소 상승시키는 결과를 보여주었다. 본 실험 연구에서 노심용융물 고압분출에 많은 영향을 미치지 않는 것으로 나타난 환형통로 면적에 대해서는 종합적으로 분석하는 추가 실험 이 필요하다.