• 한국전자통신학회논문지
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• 제12권6호
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• pp.1159-1166
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• 2017

가속도 및 자이로 센서, 아두이노 프로 미니와 블루투스 모듈을 이용하여 사물인터넷 기반의 소 행동 특성을 관찰할 수 있는 시스템을 설계하고 구현하였다. 구현된 시스템을 통해 소의 움직임을 관찰하고 측정하였으며, 측정된 데이터를 블루투스 모듈을 통해 스마트폰으로 전송하여 스마트 폰에서 소의 움직임 변화를 그래프를 통해 나타내었으며 이를 통해 소의 걸음 수를 측정하도록 하였다. 구현된 시스템과 제안한 알고리듬에 의해 소의 발정 상태를 판단하며, 이는 소의 인공수정에 의한 번식력 향상에 도움을 줄 수 있다. 본 논문에서 구현된 시스템을 실제 소의 다리에 부착하여 데이터를 측정하고, 제안한 알고리듬에 의해 발정 유무를 판단하였다. 향후 연구 과제로 피 측정되는 소의 개체수와 통신 거리를 늘려 축산 농가에서 좀 더 효율적으로 사용할 수 있는 시스템 연구 및 측정 데이터의 데이터베이스화를 하고자 한다.

• 한국수정란이식학회지
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• 제13권1호
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• pp.69-76
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• 1998

This study was carried out to establish an effective system for embryo transfer techniques by analyzing several factors affecting in-vivo embryo transfer in Korean cattle Embryos were transferred into a toral of 301 recipients. The results obtained in studies on the factors affecting pregnancy rate after embryo transfer by condition of transfer time were as follows ; 1. The pregnancy rate by the seasons of transferred fresh and frozen embryos were not different, but the pregnancy rate was slightly higher in summer(80.8%). 2. The pregnancy rate by the days of embryo transfer after estrus were not different when fresh embryos were transferred, but the pregnancy rate was highest at 8 days when frozen embryos were transferred(P<0.01, 40.0%). 3. The pregnancy rate at estrus synchronization was remarkably higher with PGF$_2$$\alpha$ treated than natural (P<0.05, 70.4%, 43.4%). 4. The pragnancy rate by the degree of estrus synchronization was best when the estrus was synchronized in both fresh and frozen embryos (83.3% and 29.7%, respectively), but the pregnancy rate was not different among $\pm$2 days. But the pregnancy rate of frozen embryos were slightly higher when the recipients exhibited estrus earlier than donors.

• 한국수정란이식학회지
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• 제33권2호
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• pp.55-59
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• 2018

Ensuring timely ovulation concerning the service is valuable. A satisfactory conception rate can be achieved by making sure that ovulation occurs within 7-18 hours after artificial insemination (AI). Delayed ovulation is one of the disturbances commonly encountered in repeat breeding animals. Although demanding research, many studies have not been conducted. Therefore, we aimed to examine the relation between ovulation confirmation and conception rate in dairy cattle. The research findings showed that the signs of true estrus were bred 12 hours after the onset of estrus by AI in cattle. Also, the performance of AI on ovulation was confirmed by the presence of fluctuant Graafian follicles through rectal palpation. From the results, we confirmed that cow encountered delayed ovulation were bred again. The Conception rate in cows with confirmed ovulation was 51.9%, while for those without confirmed ovulation were 33.3%. In conclusion, the results indicate that ovulation confirmation will likely increase conception rate.

• 한국수정란이식학회지
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• 제3권1호
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• pp.38-42
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• 1988

These studies were conducted to investigate the effect of repreated superovulation on embryo production, the effect of the frozen-thawed embryos transferred on the developmental stage and grade, and donor-recipient synchrony on pregnancy rate in Korean native cattle. The results obtained in these studies were as follows: 1. Repeated superovulations in Korean Native Catile were not affected on the number of corpus luteum (CL), embryos recovered and embryos cleaved (range: 4.8 $\pm$ 4.21 to 9.5 $\pm$ 6.50, 1.8 $\pm$ 2.53 to 8.2 $\pm$ 8.04 and 1.6 $\pm$ 2.32 to 4.0 $\pm$ 4.59, respectively). 2. Blastocyst embryos (38.5%) showed higher pregnancy rate than morula (31.6%). 3. The pregnancyrates of cattle transferred with good and fair embryos were 33.3% and 40.4%, respectively. 4. The pregnancy rate when the donors exhibited estrus 12 hours earlier than the recipients (62.5%) was higher than when the donors and recipients exhibited estrus at the same time (33.3%) or when the donors exhibited estrus 12 hours later than the recipients (20.0%).

• 한국산학기술학회논문지
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• 제21권11호
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• pp.825-832
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• 2020

소의 발정을 정확하게 탐지하는 것은 번식률을 향상시키는데 매우 중요하며, 현재 발정기 소의 행동 특성들을 활용한 다양한 발정탐지 기술들이 개발되고 있다. 본 연구에서는 우리나라 고유품종인 한우의 발정기 행동변화에 대한 기초자료를 수집하기 위하여 발정기 한우의 활동량, 승가행동 및 발성횟수 등에 대해 조사하였다. 총 4두의 한우 경산우를 공시하였으며, 각 개체별로 목걸이형 생체정보 수집장치(Bio-telemetry device)를 설치하였다. 발정이 확인된 날을 포함하여 발정 2일 전부터 발정 후 3일까지 총 6일간 이동거리, 승가행동 및 발성음을 비교 분석한 결과, 발정 2일 전부터 발정 1일 전까지 이동거리의 변화가 거의 없었으나, 발정이 확인된 당일부터 급격히 증가하였으며(p<0.001), 발정 후 1일 후부터는 서서히 감소하기 시작하였다. 또한 소의 활동량이 낮보다 밤 시간대에 더 활발한 것으로 나타났다. 반면, 승가행동은 발정 2일 전부터 발생빈도가 점점 증가하기 시작하여 발정 당일에 가장 높게 나타났으며(p<0.01), 전 시간대에 걸쳐서 불특정하게 나타나는 것을 확인할 수 있었다. 한편, 발성음의 경우 발정 당일에는 발성음 빈도가 증가하는 경향을 보였으나 유의적인 차이가 없었다(p>0.05). 발정기 한우의 승가행동은 발정 2일전부터 시작해서 발정 당일에 가장 높게 나타나는 특성이 있기 때문에 활동량보다 우선적으로 발정을 예측할 수 있는 지표로써 활용 가능할 것으로 보이며, 이 두 가지 지표를 함께 활용한다면 한우의 발정탐지율을 향상시키는데 도움이 될 것으로 판단된다. 발정탐지율을 높이기 위해서는 한우의 발정기 행동 특성과 관련하여 더욱 다양한 정보들이 필요할 것이다.

• Reproductive and Developmental Biology
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• 제37권1호
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• pp.29-33
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• 2013

The objective of this work was to analyze the concentrations of progesterone (P4) and estrogen (E2) hormones changed during estrus synchronization in dairy heifers. Estrus synchronization was carried out with CIDR$^{(R)}$ (Controlled Intravaginal Drug Release) devices. Corpus luteum (CL) was classified into three grades based on its size and palpable characteristics. The concentrations of P4 and E2 were measured by enzyme-amplified chemiluminescence. Serum P4 concentration was markedly low at the estrus stage (36 hrs after removal of CIDR) compared to other stages, while E2 concentration was kept high during estrus stage. The serum P4 concentration was highest in the CL classified into grade I. These results indicate that P4 concentration could be used as a criteria for determining recipients for artificial insemination or embryo transfer in dairy cattle.

• 한국동물생명공학회지
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• 제38권4호
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• pp.215-224
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• 2023

Background: Reproductive management practices play crucial roles to maximize the reproductive performance of cows, and thus contribute to farm profitability. We aimed to assess the reproductive management of cows currently practiced in the dairy farms in an urban farming system. Methods: A total of 62 dairy farms were randomly selected considering all size of farms such as small (1-5 cattle), medium (6-20 cattle) and large farms (> 20 cattle) from selected areas of Dhaka city in Bangladesh. The reproductive management-related parameters viz. estrus detection, breeding method, pregnancy diagnosis, dry cow and parturition management, vaccination and treatment of reproductive problems etc. were obtained in a pre-defined questionnaire during the farm visit. Results: The visual observation method was only used (100.0%; 62/62) for estrus detection irrespective of size of the farms; while farmers observed cows for estrus 4-5 times a day, but only for 20-60 seconds each time. Regardless of farm size, 89.0% (55/62) farms used artificial insemination (AI) for breeding the cows. Intriguingly, all farms (100.0%) routinely checked the cows for pregnancy at 35-40 days post-breeding using rectal palpation technique by registered veterinarian. However, only 6.5% (4/62) farms practiced dry cow management. Notably, all farms (100.0%) provided nutritional supplements (Vit D, Ca and P) during late gestation. However, proper hygiene and cleanliness during parturition was not practiced in 77.4% (48/62) farms; even though 96.7% (60/62) farms treated cows by registered veterinarian for parturition-related problems. Conclusions: While farmers used AI service for breeding and timely check their cows for pregnancy; however, they need to increase observation time (30 minutes/ observation, twice in a day: early morning and early night) for estrus detection, consider dry cow management and ensure hygienic parturition for maximizing production.

• 대한수의학회지
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• 제34권1호
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• pp.173-180
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• 1994

Milk progesterone concentrations were measured in 111 dairy cows for confirming the estrus observation and for the early pregnancy diagnosis. Of the 56 cows inseminated, 52 cows(92.8%) were an ovulatory estrus, 2 cows(3.6%) were an unovulatory estrus, and 2 cows(3.6%) were the error of estrus observation, respectively. Milk progesterone concentrations at 21 and 24 days after artificial insemination were significantly higher in 23 pregnant cows compared with those in 5 non-pregnant cows(P<0.05). The accuracy rate for early pregnancy diagnosis in 27 cows achieved when the discriminatory concentration at 21 days after artificial insemination was placed at 2.0 ng/ml skim milk, was 91.3% for positive diagnosis and 100% for negative diagnosis, respectively. These results indicated that milk progesterone determination at 0, 6 and 21 days after artificial insemination can be utilized for confirming the estrus observation and for early pregnancy diagnosis. In conclusion, milk progesterone determination is useful diagnostic tool for monitoring the reproductive performance.

• 한국수정란이식학회지
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• 제8권2호
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• pp.143-149
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• 1993

The objective of this study was to produce calves derived from in vitro fertilization of in vitro matured follicular oocytes. Oocytes aspirated from small antral folicles of ovaries obtained at a local slaughter house were matured and fertilized in vitro. At l8hrs after insemination with Korean native cattle semen, oocytes were co-cultured for 6~7 days by utilizing co-culture system with bovine oviduct epithelial cell. After co-culture, good or excellent quality late morulae or early blastocysts were selected by morphological criteria under stereo microscope. Selected embryos were transferred to recipients on day 6 or 7 (estrus = day 0). Recipients were monitored by observation for estrus and rectal palpation after 60 days from embryo transfer. One of them went to term with the birth of a calf. This case is the first production of calf derived from in vitro fertilization in Korea.

• Journal of Animal Science and Technology
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• 제65권4호
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• pp.759-766
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• 2023

Visual estrus observation can only be confirmed at a rate of 50%-60%, which is lower than that obtained using a biosensor. Thus, the use of biosensors provides more opportunities for artificial insemination because it is easier to confirm estrus than by visual observation. This study determines the accuracy of estrus prediction using a ruminoreticular biosensor by analyzing ruminoreticular temperature during the estrus cycle and measuring changes in body activity. One hundred and twenty-five Hanwoo cows (64 with a ruminal biosensor in the test group and 61 without biosensors in the control group) were studied. Ruminoreticular temperatures and body activities were measured every 10 min. The first service of artificial insemination used gonadotropin-releasing hormone (GnRH)-based fixed-time artificial insemination protocol in the control and test groups. The test group received artificial insemination based on the estrus prediction made by the biosensor, and the control group received artificial insemination according to visual estrus observation. Before artificial insemination, the ruminoreticular temperature was maintained at an average of 38.95 ± 0.05℃ for 13 h (-21 to -9 h), 0.73℃ higher than the average temperature observed at -48 h (38.22 ± 0.06℃). The body activity, measured using an indwelling 3-axis accelerometer, averaged 1502.57 ± 27.35 for approximately 21 h from -4 to -24 h before artificial insemination, showing 203 indexes higher body activity than -48 hours (1299 ± 9.72). Therefore, using an information and communication techonology (ICT)-based biosensor is highly effective because it can reduce the reproductive cost of a farm by accurately detecting estrus and increasing the rate of estrus confirmation in cattle.