• 생명과학회지
• /
• 제18권9호
• /
• pp.1186-1193
• /
• 2008

방글라데시 미멘싱에 소재하는 양식어장에서 pangasiid catfish (Pangasius hypophthalmus), silver carp (Hypophthalmichthys molitrix)와 catla (Catla catla)의 조합과 혼합비를 달리하여 16주간 복합양식하였을 때, 이들 어종의 성장률을 조사하였다. 헥타르당 30,000개체의 밀도로 복합양식하여 성장률을 측정하였으며, 혼합조성비는 다음과 같다: 실험구(treatment) 1은 pangasiid catfish와 silver carp을 1:1비율로, 실험구 2는 pangasiid catfish와 cata를 1:1비율로, 실험구 3과 4는 pangasiid catfish, silver carp과 catla를 각각 2:1:1과 3:2:1비율로 복합양식하였다. 양식환경에 영향을 주는 수온, 투명도, 용존산소량, pH, $NO_{3}-N$, $PO_{4}-P$, 엽록소 a와 같은 몇몇 물리화학적, 생물학적 요인과 플랑크톤의 군집을 정기적으로 조사하였다. 어류의 성장속도는 비 성장율(specific growth rate), 일간 체중 증가(daily weight gain)와 체중증가 비율(percent weight gain)을 측정하였다. Pangasiid catfish의 성장률과 사료이용률은 모든 실험구에서 유사하게 나타났다. Silver carp의 성장률은 모든 실험구에서 유의한 차이를 나타내었는데(p<0.05), 양식밀도가 증가할수록 성장률이 현저하게 감소하였으며, Silver carp의 밀도가 가장 낮은 복합양식 조성인 pangasiid catfish, silver carp과 catla의 비율이 각각 2:1:1일 때, silver carp의 성장률이 가장 높게 나타났다. Silver carp의 밀도가 증가할수록 catla의 성장률이 현저하게 감소하는 것으로 나타나, catla의 성장률은 silver carp의 유무에 영향을 받는 것으로 판단되었다. Catla의 성장률은 pangasiid catfish와 calta의 비율이 1:1일 때, 가장 높은 것으로 나타났다. 양식수율과 경제적 이익은 실험구에 따라 현저하게 다른 양상을 보였다(p<0.05). 가장 높은 양식수율과 경제적 이익은 pangasiid catfish, silver carp과 catla를 각각 2:1:1 비율로 양식한 결과에서 얻어졌는데, 이는 silver carp의 높은 성장률과 catla의 시장 단가가 높은 것에 기인하는 것으로 나타났다. 모든 실험구의 수질 상태는 양식기간 동안 적절한 수준을 유지하였으며, 각각의 값은 모든 실험구에서 뚜렷한 차이를 보이지 않았다(p<0.05).

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권8호
• /
• pp.1053-1061
• /
• 2004

Of the 20 arbitrarily chosen primers, six oligonucleotides decamer primers were used on the basis of the number of the polymorphisms generated in catfish (Silurus asotus) from Yesan and bullhead (Pseudobagrus fulvidraco) from Dangjin in Korea. Six primers were used generating a total of 602 scorable bands in catfish and 195 in bullhead population, respectively, ranging in size of DNA fragments from less than approximately 100 to larger than 2,000 base pairs (bp). Six primers yielded 199 polymorphic fragments (33.1%) in catfish and 47 (24%) in bullhead, respectively. In the present study, a total of 328 common fragments (an average of 54.7 per primer) were observed in catfish population, whereas 84 (an average of 14.0 per primer) in bullhead. The total number of specific fragments in catfish and bullhead population were 76 and 64, respectively. In catfish population, random decamer, OPA-17 (GACCGCTTGT) generated the highest number of fragments (a total of 141) in comparison with other primers used, with an average of 11.8. The common bands in the molecular weight of 300 bp generated by random primer OPA-06 (GGTCCCTGAC) were present in every individuals in bullhead population. The major polymorphic bands in the molecular weight of 100 bp generated by OPA-17 were identified in lane 14, 15, 17, 18, 19 20 and 21, which were identifying species in bullhead population. The average bandsharing values (BS values) of all of the samples within catfish population ranged from 0.575 to 0.945, whereas 0.063-1.000 within bullhead population. The bandsharing value (index of similarity between individuals) between individual No. 5 and No. 9 showed the highest level within catfish population, whereas the bandsharing value between individual No. 1 and No. 2 showed the lowest level. The single linkage cluster analysis resulted from four primers, indicating four genetic groupings composed of group 1 (C1-C10, all of the catfish samples), group 2 (B11, B12, B13, B14, B16, B17, B18, B19), group 3 (B15) and group 4 (B20 and B21). The dendrogram reveals close relationships between individual identities within two species populations and individuals derived from the same ancestor, respectively. However, genetic distances between two species populations ranged from 0.124 to 0.333. The shortest genetic distance (0.042) displaying significant molecular differences was between individual No. 6 and No. 9 catfish population. The shortest genetic distance (0.033) displaying significant molecular differences also was between individual No. 18 and No. 19 in bullhead population. Reversely, the genetic distance of individual No. 20/21 among individuals in bullhead population was highest (0.333). This result showed that bullhead No. 20 and 21 were distinct from other individuals within bullhead population.

• 한국식품영양학회지
• /
• 제17권2호
• /
• pp.156-162
• /
• 2004

미이용자원인 어류 carotenoid에 관한 비교 생화학적 연구의 일환으로서, 메기과의 자연산 및 양식산 메기와 한국 특산의 담수어류인 미유기의 표피 carotenoid를 TLC, column chromatography 및 HPLC로 분리하고 NaBH$_4$에 의한 환원반응, I$_2$에 의한 이성화반응 및 UV-visible spectrophotometer로 비교하고 IR 및 Mass spectrum을 측정하여 carotenoid를 동정한 결과는 다음과 같다. 자연산 메기 표피의 총 carotenoid 함량은 0.27 mg($\pm$0.7)%였으며 carotenoid의 조성은 zeaxanthin 25.8%, diatoxanin 9.7% 및 cynthiaxanthin 9.1%로서 주성분을 이루고 있었으며 그 외 isocryptoxanthin 6.5%, $\beta$-carotene 5.1%, $\beta$-cryptoxanthin 4.3%, lutein 3.4% 및 $\alpha$-cryptoxantin 2.4%의 순으로 함유하고 있었다. 양식산 메기 표피의 총 carotenoid 함량은 0.07 mg($\pm$0.03)%로서 자연산 메기에 비해 다소 낮았으며, carotenoid의 조성은 lutein 48.5%, zeaxanin 13.9% 및 isocryptoxanin 13.3%로서 주성분을 이루고, 그 외 $\beta$-carotene 9.5%, $\beta$-cryptoxantin 1.4% 및 $\alpha$-cryptoxanthin 4.6%의 순으로 함유하고 있었다. 한편 미유기 표피의 총 carotenoid 함량은 0.75 mg($\pm$0.25)%로서 메기과의 다른 어종에 비해 함량이 높았으며, carotenoid 조성은 zeaxanthin 24.5%, 7'8'-dihydro-$\beta$ -carotene-4-ol 24.1%, 7'8'-dihydro-$\beta$-carotene 17.9% 및 7'8'-dihydro-$\beta$-carotene-3-ol 10.8%로서 주성분을 이루고 있었으며 그 외 diatoxanthin 8.7%, cynthiaxanthin 6,7% 및 lutein 5.0%의 순으로 함유하고 있었다. 이러한 결과는 parasiloxanthin과 7',8'-dihyo-parasiloxanthin을 주성분으로 함유하는 biwa호수 메기, P. asotus 표피 carotenoid 조성과 비교하여, 미유기에서는 7'8'-dihydro-$\beta$-carotene의 구조를 가진 carotenoid가 존재하였으나 자연산 및 양식산 메기에서는 발견되지 않아 특이하였다.

• Applied Microscopy
• /
• 제25권2호
• /
• pp.1-10
• /
• 1995

This study was carried out to clarify the neuronal organization in pineal body of catfish. The pineal body was observed by acetylcholinesterase histochemistry and electron microscopy. The neuronal types observed in the pineal body of catfish were bipolar and multipolar cells. Multipolar cells were found throughout the pineal end vesicle and whole stalk, but bipolar cells only in the end vesicle and distal stalk. The pineal tract was formed by the long axons of these neurons. The neuronal clusters(pineal ganglia) were also observed in the end vesicle. In summary, the type of neurons in catfish pineal was different from that of other species, and the neuronal distribution differed depending on the region of pineal body. These results reflect the interspecific and regional differences of the pineal organization of fishes.

• Ecology and Resilient Infrastructure
• /
• 제2권4호
• /
• pp.337-344
• /
• 2015

The purpose of this study is to determine the effects of vibration on primary (e.g. plasma cortisol), secondary (e.g. plasma glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), $Na^+$, $K^+$ and $Cl^-$) and tertiary (e.g. mortality) stress responses in cultured catfish, Silurus asotus. For this purpose, three groups (one control group and two stress groups) were set up. The control group was exposed to vibration corresponding to 48 decibel (dB) volt (V) (produced using electric vibrators) for 15 minutes per hour every day, and the two stress groups were exposed to vibrations corresponding to 58 and 68 dB (V) (produced using the same electric vibrators), equally, for 15 minutes per hour every day. Blood was sampled at day 0 (before starting vibration stress tests), and at days 1, 3, 5, 7, 9 and 11 (after starting vibration stress tests). According to the results, the catfish physiologically showed ''typical'' stress responses when they were exposed to chronic vibration. This indicates that such chronic vibration caused substantial stress to catfish; especially, persistently elevated plasma AST and ALT levels observed caused adverse effects to them. In conclusion, chronic vibration could significantly affect hematological characteristics in catfish.

• The Korean Journal of Physiology and Pharmacology
• /
• 제2권1호
• /
• pp.21-30
• /
• 1998

The effects of GABA and glutamate on the horizontal cells were explored by an intracellular recording method to discern the mechanisms of receptive field formation by chemical coupling in the catfish outer retina. The results suggest that the horizontal cells of the catfish retina might use GABA as their transmitters and that the GABAergic system contributes to the formation of receptive fields of the horizontal cells. GABAC receptors may be involved in a chemical coupling between horizontal cells and concerned with the depolarizing actions by GABA on horizontal cells in the catfish retina. Since the chloride equilibrium potential is more positive than the dark membrane potential in horizontal cells, GABA released from a horizontal cell may depolarize the neighboring horizontal cells. Thus a chemical coupling between horizontal cells may be formed. $GABA_A$ receptors also may be involved in the negative feedback mechanism between photoreceptor and horizontal cell. And glutamate may be involved in connecting positive and negative feedback systems since it potentiated the GABA's actions. Therefore, it is presumed that large receptive fields in the catfish retina are formed not only by electrical coupling but also by chemical coupling between horizontal cells. And information travels laterally by pathways involving both electrical coupling composed of gap junctions and chemical coupling in the retinal network.

• Environmental Analysis Health and Toxicology
• /
• 제30권
• /
• pp.6.1-6.7
• /
• 2015

Objectives This study aimed to estimate the effects of 4-nonylphenol (NP), a ubiquitously present surfactant in aquatic environments, on the anti-oxidant systems of the liver in the Far Eastern catfish Silurus asotus. Methods Changes in biochemical parameters involved in glutathione (GSH)-related and other anti-oxidant systems were analyzed following 4 weeks of 4-NP administration (0.1 and 1.0 mg/kg diet) via a formulated diet to catfish. Results 4-NP exposure induced an elevation in hepatic lipid peroxide levels and an accompanying decrease in reduced state GSH after 2 weeks, suggesting pro-oxidant effects of the chemical in catfish. This oxidative stress was associated with an inhibition of the GSH-utilizing enzyme glutathione peroxidase at the same time point. This inhibition was restored after 4 weeks. The activities of other anti-oxidant enzymes, i.e., glutathione reductase, superoxide dismutase and catalase were increased after 4 weeks. These enzyme increases occurred more strongly at the higher 4-NP concentration (1.0 mg/kg diet). Conclusions 4-NP given to catfish at 0.1 to 1.0 mg/kg diet, concentrations relevant to environmental levels, depletes the endogenous anti-oxidant molecule GSH and temporarily inhibits GSH-related anti-oxidant enzymes. Such declines in anti-oxidant capacity and elevated oxidative stress seem to be compensated eventually by subsequent activation of various anti-oxidant enzyme systems.

• 한국어류학회지
• /
• 제18권3호
• /
• pp.209-215
• /
• 2006

한국의 퉁가리과 어류 3종의 형태학적 특성을 classical dimension과 truss dimension을 사용하여 파악하였다. Arc sin square root 전환 후, 18가지의 계측형질을 체장에 대한 비율로, 그리고 3가지의 계측형질은 두장에 대한 비율로 각각 분석하였다. 퉁사리, Liobagrus obesus는 classical dimension과 truss dimension의 4가지 계측 형질이 퉁가리, L. andersoni와 자가사리, L. mediadiposalisa에 비하여 컸다 (P<0.05). 퉁사리와 퉁가리는 truss dimension의 3가지 계측형질이 자가사리보다 컸다 (P<0.05). 조사된 퉁가리과 어류의 6가지 계측형질에서는 유의적인 차이가 없었다. 본 연구에 사용된 dimension들은 한국산 퉁가리과 어류들을 구별할 수 있는 적절한 분류학적 지표로 사용될 수 있으리라 사료된다.

• 한국환경농학회지
• /
• 제42권4호
• /
• pp.408-417
• /
• 2023

This study aimed to investigate the effects of an aquaponics system utilizing catfish recirculating water on the growth and water quality in lettuce and chicory cultivation. After 60 d of catfish cultivation in the BFT-AP, the survival rate was 96.0% and average weight gain was 178.5 g. The WGR grew by 241.4% and the SGR was 3.0%. A comparison between lettuce and chicory cultivation using BFT-AP and hydroponics revealed significant increases in shoot height, leaf count per week, and biomass of the cultivated lettuce and chicory. The SPAD values also increased significantly by 15.4-43.3%. Water quality analysis of the BFT-AP systems and crop cultivation beds showed significant differences, except for K and Na. The average NO₃^- concentration in the sediment pond was significantly higher at 45.3±1.0. Comparison of water temperature, pH, EC, DO, and ORP in the BFT-AP system and crop cultivation beds revealed similar monthly water temperatures from July to September. The pH in the catfish culture pond was 0.3-1.2 lower than that in the crop cultivation beds, and the EC was approximately 0.01- 0.03 dS/m lower in the catfish culture pond than the crop cultivation beds. The monthly average ORP in the catfish culture bed steadily increased to 479.9 mV in October. Bacillus velezensis (AFB2-2) and Chlorella fusca (CF) were applied to the biological activation bed; the population density of CF showed a continuous increase, whereas that of AFB2-2 decreased after 15 d and gradually increased thereafter. Thus, the application of the recirculating aquaponics cultivation technology with catfish (BFT) enhances catfish cultivation and promotes the growth of lettuce and chicory. Therefore, it is considered suitable for the environment-friendly cultivation of lettuce and chicory.

• The Korean Journal of Physiology
• /
• 제27권2호
• /
• pp.163-174
• /
• 1993

Vertebrate retinal neurons, like brain tracts farm complex synaptic relations in the enter and inner plexiform layers which ape equivalent to the central nervous system nuclei. The effects of $\gamma-aminobutyric$ acid(GABA) and glycine on retinal neurons were explored to discern the mechanisms of action of neurotransmitters. Experiments were performed in the superfused retina-eyecup preparation of the channel catfish, Ictalurus punctatus, using intracellular electrophysiological techniques. The roles of GABA and glycine as inhibitory neurotransmitters are well established in the vertebrate retina. But, we found that the depolarizing action of GABA and glycine on third-order neurons in the catfish retina. GABA and glycine appeared to act on retinal ueurons based on the observations that (1) effects on photoreceptors were not observed, (2) horizontal cells were either hyperpolarized $({\sim}33%)$ or depolarized $({\sim}67%)$, (3) bipolar cells were all hyperpolarized (4) amacrine and ganglion cells were either hyperpolarized $({\sim}37%)$ or depolarized $({\sim}63%)$, (5) GABA and glycine may be working to suppress presynaptic inhibition. The results suggest that depolarization of third-order neurons by GABA and glycine is due to at least two mechanisms; a direct postsynaptic effect and an indirect effect. Therefore, in the catfish retina, a mechanism of presynaptic inhibition or disinhibition including the direct postsynaptic effect may exist in the third-order neurons.