• 대한치과의사협회지
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• 제12권3호
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• pp.155-158
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• 1974

Catecholamine levels were determined in the submandibular glands of new born rats treated with physiologieal saline solution or large doses of sodium fluoride (10mg/kg) for verying periods of time. In control group, catecholamine levels were gradually increased from fifth day of life until twenty-fifth day of life. When fluoride treatment was started on the fifth day after birth and continued daily to the thirty-fifthday of life, decreased catecholamine levels were obsrved on the fifth, through all experiments, and thirth-fifth day. Daily fluoride treatment from the fifteenth through the thirty-fifth day after birth induced the decreased catecholamine levels which were higher than the levels in submandibular glands of rats treated from the fifth day to the thirty-fifth day after birth with NaF. These experiments suggest that fluoride may be affecting catecholamine biosynthetic mechanisms which were duveloping during the fifteenth to twenty-fifth day of life.

• Environmental Analysis Health and Toxicology
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• 제3권3_4호
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• pp.9-15
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• 1988

The word of stress crime from Latin language as stringere and it was used in medical fields from 1935. According to Selye, all the biological bodies reveal physilolgical changes when some stimulation exceed normal levels, and consequently the pituitary gland and adrenal systems are activated. Jacob expressed that stress is the loss of homeostasis by physical, chemical, and emotional stimulation. When biological organisms receive extreme stress the amount of catecholamine excretion are increase. Author investigated the catecholamine contents in rat urine after giving the low temperature stress, noise stress, and water immersion stress. The 24 hours rat urine was collected by adding 1 ml 6 N-HCl and the sample is passed through Bio-Rex 70 samples treatment column to extract catecholamine and detected the catecholamine with HPLC-fluorescence detetor. The highest epinephrine concentration was 67.14 ng in water immersion stress condition and the dopamine concentration of 221.37 ng was shown in the low temperature stress condition.

• Journal of Preventive Medicine and Public Health
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• 제26권4호
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• pp.565-573
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• 1993

Noise is not only affecting the ear and the auditory cortex locally, but its influence is widely spread throughout the brain structures, e. g., the reticular formation, the brain stem nuclei or the subcortical forebrain area. Hence, any of the organism's activities can be hindered or stimulated by noise. High noise is a stressor and the catecholamine level can be used both as a stress marker and as an indicator of modified sympathetic nervous system activity. Several recent studies have found that the urinary excretion of catecholamines is increased due to high noise intensity, especially unexpectedly high and long lasting noise. The present study was conducted in order to examine the effects of noise stress on urinary excretion of ctecholamines in rats and humans. Rats were exposed to 90 dB noise for 10, 30, and 60 minutes, 3 and 12 hours. 24 hour . urinary samples were collected and the catecholamones were extracted by alumina and analyzed by HPLC-ECD. Catecholamine levels increased with time of exposure up to 60 minutes : norepinephrine concentration at 60 min of noise=1.038 ng/ml, epinephrine=0.636 ng/ml. Urine catecholamines of blue collar workers exposed to 90 dB of noise at the work place were collected between 2 and 4 p.m. and compared to that of white collar workers exposed to 70 dB. Mean norepinephrine level of the blue collar workers was 0.89 ng/ml (${\pm}0.25$), epinephrine 0.24ng/m1 (${\pm}0.09$), and that of the white collar workers 0.48 ng/ml (${\pm}0.12$), epinephrine 0.19 ng/ml(${\pm}0.05$). It was concluded that noise acts as a stressor and increases the catecholamine levels in both rats and humans.

• Nutrition Research and Practice
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• 제4권4호
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• pp.270-275
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• 2010

Catecholamines are among the first molecules that displayed a kind of response to prolonged or repeated stress. It is well established that long-term stress leads to the induction of catecholamine biosynthetic enzymes such as tyrosine hydroxylase (TH) and dopamine ${\beta}$-hydroxylase (DBH) in adrenal medulla. The aim of the present study was to evaluate the effects of ginseng on TH and DBH mRNA expression. Repeated (2 h daily, 14 days) immobilization stress resulted in a significant increase of TH and DBH mRNA levels in rat adrenal medulla. However, ginseng treatment reversed the stress-induced increase of TH and DBH mRNA expression in the immobilization-stressed rats. Nicotine as a ligand of the nicotinic acetylcholine receptor (nAChR) in adrenal medulla stimulates catecholamine secretion and activates TH and DBH gene expression. Nicotine treatment increased mRNA levels of TH and DBH by 3.3- and 3.1-fold in PC12 cells. The ginseng total saponin exhibited a significant reversal in the nicotine-induced increase of TH and DBH mRNA expression, decreasing the mRNA levels of TH and DBH by 57.2% and 48.9%, respectively in PC12 cells. In conclusion, immobilization stress induced catecholamine biosynthetic enzymes gene expression, while ginseng appeared to restore homeostasis via suppression of TH and DBH gene expression. In part, the regulatory activity in the TH and DBH gene expression of ginseng may account for the anti-stress action produced by ginseng.

• BMB Reports
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• 제29권2호
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• pp.142-145
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• 1996

To investigate the cause of increased plasma catecholamine levels in liver disease, catechol-O-methyltransferase (COMT), which provides a major route of catabolism for circulating catecholamines, was studied under the cholestasis induced by mechanical biliary obstruction in rats. Monoamine oxidase (MAO) activity and the $K_m$ and $V_{max}$ values for both enzymes were also measured. Cytosolic, microsomal, and mitochondrial COMT activities in the cholestatic liver were significantly decreased throughout the experiment. Microsomal, and mitochondrial MAO activity in the cholestatic liver were also significantly decreased. Vmax values of COMT and MAO were lower. Serum COMT and MAO activities were detected after CBD ligation. These results indicate that plasma catecholamine levels are increased in liver disease due to decreased hepatic degradation of catecholamines by decreased activities of COMT and MAO. The decreased activity of these enzymes is caused by decreased biosynthesis and by flowage into the blood from the damaged hepatocyte.

• 대한간호학회지
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• 제36권7호
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• pp.1123-1134
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• 2006

Purpose: The purpose of this study was to identify the effects of aromatherapy on blood pressure and stress responses of clients with essential hypertension. Method: There were fifty-two subjects divided into an essential oil group, placebo group, and control group by random assignment. The application of aromatherapy was the inhalation method of blending oils with lavender, ylangylang, and bergamot once daily for 4 weeks. To evaluate the effects of aromatherapy, blood pressure and pulse were measured two times a week and serum cortisol levels, catecholamine levels, subjective stress, and state anxiety were measured before and after treatment in the three groups. Data was analyzed by repeated measures of ANOVA, one-way ANOVA, and $X^2-test$ using the SPSS 10.0 program. Results: The blood pressure, pulse, subjective stress, state anxiety, and serum cortisol levels among the three groups were significantly statistically different. The differences of catecholamine among the three groups were not significant statistically Conclusion: The results suggest that the inhalation method using essential oils can be considered an effective nursing intervention that reduces psychological stress responses and serum cortisol levels, as well as the blood pressure of clients with essential hypertension.

• 한국식생활문화학회지
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• 제24권2호
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• pp.236-243
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• 2009

This study was performed to investigate the effects of aluminum sulfate administration on the brain tissues of old rats, when given at different concentrations. The experiment attempted to further ascertain whether aluminum exposure cause Alzheimer's disease. Seventy-five aged Sprague-Dawley rats were divided into five groups; a control group, 2 ppm aluminum sulfate group, 20 ppm aluminum sulfate group, 40 ppm aluminum sulfate group, and 200 ppm aluminum sulfate group, and were kept on the respective diets for 12 weeks. In order to understand the influence of aluminum on the brain, serum aluminum concentrations, phospholipid composition, and catecholamine concentrations were compared between the aluminum-treated groups and the normal group. According to the results, serum aluminum was higher in the aluminum sulfate-treated groups than in the normal group. Within the cortex, catecholamine concentrationes were significantly increased but cerebellum and brainstem tissue were significantly decreased, in the aluminum sulfate-treated groups compared to the normal group. For phospholipid composition, phosphatidyl inositol was significantly increased wherase phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl serine were significantly decreased in the aluminum sulfate-treated groups versus the normal group. Based on the data, increased aluminum consumption in experimental animals causes increased serum aluminum levels and catecholamine variation. These phenomena are very similar to conditions of Alzherimer's disease. Therfore, the results of this experiment further suggest that aluminum cause Alzherimer's disease, coinciding with reports that aluminum is a cause of neurofibrilly tangles in the brain.

• 생약학회지
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• 제39권4호
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• pp.341-346
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• 2008

The effects of the ethanol extracts from Gynostemma pentaphyllum (GP extracts) on body weights, grip strengths, endurances and catecholamine levels after electric footshock (EF) stress in mice and rats were investigated. The animals were treated with GP extracts (50 mg/kg/day, p.o.) for 21 days before exposure to EF (duration and interval 10 sec for 3 min, 2 mA) once a day. The increases in body weights were delayed by 13.1% of the control levels by EF-induced stress in mice, which were recovered to 24.1% of the control levels in GP extract-treated groups. The grip strengths were significantly decreased by EF stress in mice and the EF-stressed groups treated with GP extracts increased grip strengths to 115.2% compared to control levels. The endurance times by forced swimming, which reduced significantly by EF stress, were also maintained similar to control levels by GP extracts in rats. In addition, the levels of norepinephrine and epinephrine in serum and brain, and dopamine in brain were significantly increased to 17.5-95.0% of the control levels after exposure of EF stress in mice. However, EF stressinduced increases in norepinephrine and epinephrine in serum were reduced to 17.1-17.3% of the control levels by treatments of GP extracts, and those in dopamine, norepinephrine and epinephrine in brain were also reduced to 5.0-19.5%. These results suggest that GP extracts showed the protective effects on EF stress-induced physiological functions and can be developed as the promising anti-stress agents.

• 대한한의학회지
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• 제16권2호
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• pp.433-446
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• 1995

The present study was carried out to investigate the effect of water extract and 70% ethanol extract from Gastrodiae Rhizoma on cardiovascular activities and plasma levels of catecholamines in unanaesthetized spontaneously hypertensive rats. The depressor response in SHR was observed during three to six hour period after an oral administration of water extract from Gastrodiae Rhizoma(GR). There was a statistically significant correlation between the magnitude of the depressor response induced by an oral administration of water extract from GR and the initial control blood pressure level. The increase in blood pressure induced by norepinephrine was less in Wistar rat treated with GR water extract than those without GR extract. No significant change in heart rate was observed in SHR receiving either water extract or ethanol extract from GR. Associated with the depressor response, there was a concomitant reduction in plasma levels of norepinephrine in SHR at 4 hour after an oral administration of water extract from GR. Plasma levels of norepinephrine and epinephrine were decreased slightly at 2 hour after an oral administration of ethanol extract from GR. These results suggest that the depressor effect of water extract from GR may be due, in part, to a decreased sympathoadrenal activity.

• 대한한의학회지
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• 제17권1호
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• pp.146-170
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• 1996

This study aimed to evaluate the anti-stress effects of Walgukhwan, Walgukhwan+Ejintang and Wal gukhwan+Samultang. The experimental animals treated with each extracts during 15 days before the immobilization stress. And each experimental animals were endowed with 12 hours immobilization stress. Then the brain, blood and urine catecholamine contents and serum total cholesterol, triglyceride and free fatty acid levels were studied. The result were as followed; 1. Walgukhwan, Walgukhwan+Ejintang and Walgukhwan+Samultang were not statistically effective to the changes of the body weight. 2. Walgukhwan was statistically effective to the increase of the blood norepinephrine content as compared with control. 3. Walgukhwan+Ejintang was statistically effective to the increase of the blood and urine norepinephrine contents as compared with control. 4. Walgukhwan+Samultang was statisticallv effective to the increase of the brain, blood and urine norepinephrine contents and also to the increase of the blood and urine dopamine contents as compared with control. 5. Walgukhwan was statistically effective to the increase of the serum free fatty acid level as compared with control. 6. Walgukhwan+Ejintang was statistically effective to the increase of the serum triglyceride and free fatty acid levels as compared with control. 7. Walgukhwan+Samultang was statistically effective to the increase of the serum total cholesterol, triglyceride and free fatty acid levels as compared with control.