• Korean Journal of Veterinary Research
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• v.38 no.2
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• pp.273-279
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• 1998

The ability of bovine intact red blood cells to scavenge superoxide and hydrogen peroxide by superoxide dismutase, catalase and glutathione peroxidase was investigated. Intact red cells(up to 0.4%) suspensions did not inhibit ferricytochrome c reduction by superoxide in the superoxide generating system. On the other hand, intact red cell(0.4%) suspensions almost completely inhibit ferrocytochrome c oxidation by hydrogen peroxide. The ability of intact red cells to scavenge hydrogen peroxide was mainly attributed to either membrane bound catalase or glutathione peroxidase. The scavenge of hydrogen peroxide by 0.1~0.2% intact red cells showed a trend of dependence on mainly glutathione peroxidase. However, at blood cell concentration higher than 0.3%, the process depended upon peroxidase-independent scavengers like catalase. Enhancement of ferrocytochrome c oxidation by red cells treated with aminotriazole proved that the protection against hydrogen peroxide was due to catalase, while the protection in the presence of glutathione indicated scavenging effect of glutathione peroxidase against hydrogen peroxide.

• BMB Reports
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• v.36 no.6
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• pp.608-610
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• 2003

Escherichia coli has two catalases, HPI and HPII. HPI is induced during logarithmic growth in response to low concentrations of hydrogen peroxide. This induction is OxyR-dependent. On the other hand, HPII is not peroxide-inducible but is induced in entry to the stationary phase. We demonstrate here that E. coli displayed higher HPI catalase activity when compared to the cultures that were grown in a normal medium, if grown in a medium supplemented with iron-citrate. Iron supplementation had no effect on HPII catalase. This increase of HPI activity was OxyR-independent and not observed in a ${\Delta}fur$ mutant. The physiological significance of the increase of HPI activity is unclear, but it appears that the katG gene that codes for HPI catalase is among the genes that are regulated by Fur.

• The Korean Journal of Zoology
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• v.37 no.1
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• pp.97-103
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• 1994

Catalase 억제제로 알려져 있는 AT가 Pn에 중독된 생쥐 생존율을 유의하게 감소시켰다. 생쥐의 간장 폐 및 신장에서 catalase 활성도가 현저히 감소되었으며, PQ와 AT 병합투여군에서 Catalase 활성은 PQ 단독투여 군에 비하여 유의한 감소를 나타냈으나 glutathione량과 SOD 활성은 PQ 단독투여군과 유의한 차이를 나타내지 않았다. 또한 광학현미경 검색상 PQ 단독투여 군에서 사구체와 요세관이 손상되었으나 AT의 병합투여군에서 사구체와 요세관의 손상이 더욱 증가됨을 보여주고 있다. 이상의 실험 결과로 PQ의 신독성이 AT 병합투여로 더욱 증가됨을 알 수 있는데 이러한 결과는 AT가 신장 Catalase를 억제하여 증가된 과산화수소에 의해 나타난 독성의 결과로 생각되며, 과산학수소는 Haber-Weiss 반응이나 trenton 반응 또는 paraquat radical과 직접 반응하여 hydroxvl radicals을 생성하므로 PQ 독성은 과산화수소의 생성과 관련이 있는 것으로 추정된다.

• Microbiology and Biotechnology Letters
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• v.16 no.3
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• pp.193-198
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• 1988

Catalase from Aspergillus niger KUF-04 was purified by five steps including gel filtration. The overall purification gave 64-fold purified preparation, a yield of about nine percent. The enzyme showed its maximum absorption at 406 nm. The optimum pH and temperature for the enzyme activity were around pH 7.0 and 6$0^{\circ}C$, respectively. The catalase was found to be stable in the range of pH 4.0 to pH 8.3 and temperature 2$0^{\circ}C$ to 6$0^{\circ}C$. However, it lost nearly all of the activity by heating at 8$0^{\circ}C$ for 20 min. The activity was markedly inhibited by hydroxylamine, potassium cyanide and sodium azide.

• Biomolecules & Therapeutics
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• v.7 no.2
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• pp.121-126
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• 1999

In an attempt to define the effects of hyperbaric oxygen treatment on the lipid peroxidation and oxygen free radical reactions in rats exposed to carbon monoxide, we studied malondialdehyde (MDA) level and activities of catalase and superoxide dismutase in the kidney of the rats exposed to carbon monoxide. Male Sprague-Dawley albino rats weighing 240 to 260 gm were used. Experimental groups consist of Control group (=breathing with air), HBO group (=exposed to hyperbaric oxygen 〔HBO, 3ATA, 100%〕 after air breath), CO group (=exposed to CO〔3,970 ppm〕after air breath), CO-Air group (=exposed to CO after air breath followed by air breath) and CO-HBO group (=exposed to CO after air breath followed HBO treatment). The CO group showed significantly higher MDA level, catalase activity and SOD activity as compared to that of control group. The CO-HBO group showed significantly lower MDA level as compared to that of CO group, and did not show significantly lower catalase activity and SOD activity as compared to that of CO group. These results suggest that the excessive oxygen free radicals is an important determinant in pathogenesis of CO-induced nephrotoxicity and HBO inhibits the lipid peroxidation caused by excessive oxygen free radicals in the kidney of the rats exposed to carbon monoxide.

• Toxicological Research
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• v.13 no.4
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• pp.393-400
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• 1997

The effects of superoxide dismutase(SOD) and catalase on the toxicity of paraquat(PQ) were studied using diethyldithiocarbamate(DDC), 3-amino-1,2,4-triazole(AT) which are inhibitors of Cu, Zn-SOD and catalase in rats. Sprague Dawley rats were divide into 6 groups: control, DDC, PQ, AT, DDC+PQ, and AT+PQ group. The PQ (50 mg/kg body weight(BW); about half dose of $LD_{50}$) was administered with orally, otherwise AT(1.0g/kg BW) and DDC(1.0g/kg BW) were administered by intrperitoneal(iP) injection. The survival rate of rats in PQ+AT group was significantly decreased compared with PQ group while the difference of survival rate between DDC group and DDC+PQ group was not significant. The SOD activity after administration of DDC was decreased in liver, lung and kidney, but catalase activity was not changed. The catalase activity in liver, lung and kidney of AT treated rats was decreased, while SOD activity was not changed in this group. The effects of DDC and AT to the PQ toxicity was also observed in primary cultured rat Skin fibroblasts. The viable cells that was measured with MTT method, was decreased in AT+PQ treated group compared to PQ treated group, but the difference of cell viability between DDC treat group and DDC+PQ treated group was not observed. This result, AT potentlate PQ toxicity while DDC were not affect, suggested that the decreased catalase activity lead to elevation of hydrogen peroxide levels and PQ toxicity may be correlate with the hydrogen peroxide rather than the superoxides.

• Proceedings of the Ginseng society Conference
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• 1998.06a
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• pp.63-70
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• 1998

Superoxide dismutase (SOD) and catalase constitute the first coordinated unit of defense against reactive oxygen species. Here, we examined the effect of ginseng saponins on the induction of SOD and catalase gene expression. To explore this possibility, the upstream regulatory promoter region of Cu/Zn superoxide dismutase (SODI) and catalase genes were linked to the chloramphenicol acetyl-transferase (CATI structural gene and introduced into human hepatoma HepG2 cells. Total saponin and panaxatriol did not activate the transcription of SODI and catalase genes but panaxadiol increased the transcription of these genes about 2-3 fold. Among the Panaxadiol ginsenosides, the Rb2 subtraction appeared to is a major induce of SODI and catalase genes. Using the deletion analyses and mobility shift assays, we showed that the 5051 gene was greatly activated by ginsenoside Rba through transcription factor AP2 binding sites and its induction. We also examined the effect of the content ratio of panaxadiol extracted from various compartment of ginseng on the transcription of 5031 gene. Saponin extract that contains 2.6-fold more PD than PT from the fine root Increased the SODI induction about 3-fold. These results suggest that the panaxadiol fraction and its ginsenosides could induce the antioxidant enzymes, which are important for maintaining cell viability by lowering level of oxygen radical generated from intracellular metabolism.

• Korean Journal of Animal Reproduction
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• v.20 no.3
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• pp.315-322
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• 1996

The study was conducted to determine the optimal glucose, superoxide dimutase(SOD) and catalase levels during the in vitro culture of porcine oocytes matured and fertilized in vitro for morulae and blastocyst development. Oocytes were cultured for 0~8 days in TCM-199 medium supplemented with 20% FCS, different glucose, SOD and catalase levels. The results are summairzed as follows ; 1. The in vitro developmental rates of porcine oocytes cultured in TCM-199 medium containing 0.1, 0.3, 0.5, 1.0, 3.0 mM glucose levels 0~3 and 0~8 days after insemination were 22.8, 24.2, 21.9, 20.0, 12.1 and 21.9, 26.7, 25.0, 22.6, 16.7%, respectively. 2. The in vitro developmental rates of porcine oocytes cultured in TCM-199 medium containing 100, 200, 300, 500 $\mu\textrm{g}$/ml SOD levels 0~3 and 0~8 days after insemination were 16.7~23.3 and 16.7~25.0%, respectively. High levels of SOD(500 $\mu\textrm{g}$/ml) significantly reduced the rates of molurae and blastocysts stage(P<0.05). 3. The in vitro developmental rates porcine oocytes cultured in TCM-199 medium containing 100, 200, 300, 500 $\mu\textrm{g}$/ml catalase levels 0~3 and 0~8 days after insemination were 18.8~26.7 and 19.4~28.1%, respectively, and there was significant differences on the development to the molurae and blastocysts stage among the cumulus cells and glucose levels.

• Journal of Life Science
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• v.20 no.4
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• pp.511-518
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• 2010

Exercise-induced anaphylaxis (EIA) is defined as the onset of allergic symptoms during, or immediately after, exercise, the clinical signs being various degrees of urticaria, angioedema, respiratory and gastrointestinal signs, and even anaphylactic shock. Food-dependent exercise-induced anaphylaxis (FDEIA) is a specific variant of exercise-induced anaphylaxis that requires both vigorous physical activity and the ingestion of specific foods within the preceding several hours. To describe the physiopathologic mechanism, etiologic factors, and clinical manifestations, we evaluated the supplementation of vitamin C and catalase on spleen index, proliferation assay, ROS, and ASAS in sensitized and exercise trained mice. The results were as follows: Spleen index showed the highest level in the ST12 group compared to other groups; this level increased in a time dependent manner and in significant amounts. In proliferation assay of Med and OVA, the ST12 group showed the highest level compared to other groups; this level also increased in a time dependent manner. On the other hand, spleen ROS did not show a statistically significant difference, and peritoneal ROS showed the highest level in the ST12 group. ASAS showed the highest level in the ST12 compared to the S; this was also in a time dependent manner and in significant amounts. From the results, we chose the ST9 and ST12 groups to evaluate allergy anaphylaxis with supplementation of Vitamin C and catalase. In both the ST9 and ST12 groups, peritoneal ROS and ASAS were lower in vitamin C treatment group than in the catalase treatment group. This was a statistically significant difference. From the results, allergy anaphylaxis showed a higher level in the long trained group than in the short trained group. Also, treatment with vitamin C was more effective in lowering allergy anaphylaxis than catalase treatment.

• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.93-100
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• 1997

Plants are exposed to wide range of different stresses. As plants have only limited mechanism for stress avoidance, they require flexible means for adaption to changing environmental conditions. This study was carried out to reasearch the changes of antioxidant enzymes activities as caused by water stress in four lettuceUactuca sativa) lines. Four lettuce lines exposed to water stress showed premature senescence as evidenced by the consistenent reduction in the content of total soluble protein and total lipid. Water stress also caused decreased activities of superoxide dismutase, catalase, ascorbate peroxidase, but decrease rates were different. Catalase activity was decreased much more than that of ascorbate peroxidase that suggest catalase reacted with hydrogenyperoxide directly not with ascorbate peroxidase.