• Title/Summary/Keyword: Catabolism

검색결과 153건 처리시간 0.026초

노래미, Hexagrammos agrammus의 섭식양과 성장양으로부터 에너지 대사의 추정 (Estimation of Energy Budget from Food Consumption and Growth of Hexagrammos agrammus)

  • 김종관;장창익
    • 한국수산과학회지
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    • 제31권1호
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    • pp.121-126
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    • 1998
  • 본 연구는 von Bertalanffy의 성장식과 섭식양을 이용하여 자연조건하에서 노래미, Hexagrammos agrammus의 에너지 대사를 파악하기 위하여 시도된 것이며, 표본은 부산 동백섬 연안과 삼천포 신수도 연안에서 채집된 것이다. 에너지 대사 모델은 섭식양, 함장양, 흡수양, 동화양, 이화양, 배설양, 불소화배출양 등으로 구성된 표준화된 에너지 수지 모델을 이용하였으며, 에너지 대사의 구성요소별 측정단위는 열량단위가 아닌 중량단위로서 나타내 었다. 노래미에 의해 섭식된 연간섭식양이 에너지 대사의 구성요소별 배분되는 양 또는 을은 어체의 연령에 따라 달랐다. 에너지 대사의 구성요소별 추정값을 연령군별로 비교한 결과, 연간배설양은 어체의 연령이 증가할수록 감소하는 경향을 나타내었고, 나머지 구성요소는 어체의 연령이 증가할수록 증가하였다. 연령군별 평균체중 (W)과 연간흡수양($A_b$) 및 연간리화양(R) 간에는 각각 $A_b=4.592W^{0.666}$의 비선형과 R=0.007+0.567W의 선형관계가 있었고, 연간섭식양 (C)과 연간동화양 (A) 및 연간리화양 간에는 각각 A=-7.026+0.061C와 R=-20.749+0.048C의 선형관계가 있었다.

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Dunaliella tertiolecta의 포도당산화와 산화효소계 (II) Cell-free Extracts를 사용한 Glycolytic 및 Pentose Phosphate Pathway의 존재확인 (Glucose Oxidation and It's Oxidative Enzyme Systems in Dunaliella tertiolecta. (II) Evidence for Glycolytic and Pentose Phosphate Pathways in Cell-free Extracts)

  • 권영명
    • Journal of Plant Biology
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    • 제12권2호
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    • pp.15-22
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    • 1969
  • By spectrophotometric assay method, the following enzymes could be detected in Dunaliella tertiolecta and Chlorella pyrenoidosa cell-free extracts: Hexokinase; Glucose-6-phosphate, 6-Phosphogluconate and Triosephosphate dehydrogenase; Transketolase; Phosphogluco and Ribosephosphate isomerase; Phosphoglucomutase; Phosphofructokinase; Fructosediphosphate aldorase and Ribulosephosphate 3-epimerase. Such enzymes are in accordance with the proposed pathway of glucose catabolism by D. tertiolecta as well as C. pyrenoidosa. Also, it could be estimated, under the presence of NADP, that pentose phosphate pathway were more active than glycolytic pathway in D. tertiolecta cell-free systems.

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Total synthesis of 1,4-Dideoxy-1,4-Imino-D-Arabinitol(DABl)

  • Kim, In-Su;Jung, Young-Hoon
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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    • pp.178.2-178.2
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    • 2003
  • Naturally occurring sugar mimics with a nitrogen in the ring are classified into five structural classes: polyhydroxylated pyrrolidines, piperidines, indolizidines. pyrrolizidine, and nortropanes. Glycosidase are involved in a wide range of important biological processes, such as intestinal digestion, post-translational processing of glycoproteins and the lysosomal catabolism of glycoconjugate. The realization that alkaloidal sugar mimics might have enormous therapeutic potential in many diseases such as viral infection, cancer and diabetes has led to increasing interest and demand for these compounds. (omitted)

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Amino Acid Imbalance-Biochemical Mechanism and Nutritional Aspects

  • Park, Byung-Chul
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권9호
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    • pp.1361-1368
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    • 2006
  • Amino acid imbalances refer to the deleterious effects that occur when a second-limiting amino acid or mixture of amino acid lacking a particular limiting amino acid is supplemented in diets marginal in one or more indispensable amino acids. In spite of variation in the conditions that have been used to induce amino acid imbalances, such as protein level in the diet, the extent of difference in total nitrogen content between basal and imbalanced diets, and kinds of amino acids used as imbalancing agents, the conspicuous common features of amino acid imbalances have been a decreased concentration of the limiting amino acid in blood, depression of feed intake and weight gain, and increased dietary content of the limiting amino acid needed to correct the imbalances. There is strong evidence that a decrease in the concentration of a limiting amino acid detected in the anterior prepyriform cortex of the brain is followed by behavioral effects, especially a decrease in feed intake. This might be due to the competition between the limiting amino acid and the amino acids in the imbalancing mixture for transport from blood into brain. One of the biochemical responses of animals fed amino acid imbalanced diets is a rapid decrease in the concentration of the limiting amino acid, which are due in part to an increase in catabolism of the limiting amino acid by the increased activities of enzymes involved in the catabolism of the amino acid. Practically, specific amino acid imbalances could be induced in swine and poultry diets that have been supplemented with lysine, methionine, tryptophan when threonine, isoleucine, valine, etc. are potentially third- or fourth-limiting in diets. In these cases supplementation of the limiting amino acid could be beneficial in preventing the decrease of feed intake that could otherwise occur as a result of amino acid imbalance.

A Putative Early Response of Antifungal Bacillus lentimorbus WJ5 Against the Plant Pathogenic Fungus, Colletotrichum gloeosporioides, Analyzed by a DNA Microarray

  • Lee Young-Keun;Jang Yu-Sin;Chang Hwa-Hyoung;Hyung Seok Won;Chung Hye-Young
    • Journal of Microbiology
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    • 제43권3호
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    • pp.308-312
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    • 2005
  • The global RNA transcription profiles of Bacillus lentimorbus WJ5 under an in vitro co-culture with Colletotrichum gloeosporioides were analyzed in order to study the antagonistic bacteria-fungi interactions. Using a filter membrane system, B. lentimorhus WJ5 was exposed to the spores of C. gloeosporioides at the late exponential stage. The transcription profiles of the B. lentimorhus WJ5, both with and without a challenge from C. gloeosporioides, were analyzed using custom DNA chips containing 2,000 genome fragments. A total of 337 genes were expressed, with 87 and 47 up- and down-regulated, respectively. Of these, 12 genes, which were involved in central carbon metabolisms, and 7 from minor catabolism were relatively highly up-regulated (> 10 fold) and down-regulated (< 0.2 fold), respectively. Nine genes, which were thought to be related to the antifungal activity, were also up-regulated, but their levels were not so high (2.0 - 9.7 folds). From the results, during the early stage of the co-culture of B. lentimorbus WJ5 and C. gloeosporioides, nutrient competition seemed to occur; therefore, the genes from central carbon metabolisms could be up-regulated, while those from minor catabolism could be down-regulated.

Heat shock protein의 기능과 면역 반응 (Function of heat shock protein and Immune response)

  • 김세진
    • 미생물과산업
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    • 제25권1호
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    • pp.2-9
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    • 1999
  • A study was made on enzymes of carbohydrate metabolism in T. concretivorus grown with and without glucose. The present results show that T. concretivorus possesses high activities of pentose shunt pathway and related enzymes, glucokinase, G-6-P dehydrogenase, 6-PG dehydrogenase, and phosphoglucoisomerase, but low activities of enzymes unique to EMP(fructose-1,6-diphosphate aldolase). Although the synthesis of the latter enzymes remains largely unaffected by the growth enviroment, that of the former is stimulated by glucose. And the failure to detect ED pathway enzymes in cells grown in thiosulate or thiosulfate-glucose medium eliminates the ED pathway as a significant route of glucose catabolism in T.concretivorus. These results suggest that pentose shunt pathway performs an energetic role in glucose metabolism by T.concretivorus with EMP as a subway. The absence of ED pathway and the presence of pentose shunt pathway which is the major route of catabolism in T.concretivorus are similar to those of other obligately chemolitho-trophic thiobacilli. The G-6-P and 6-PG dehydrogenase are both NAD and NADP specific, but MAD predominant. However, the 3-PGAL dehydrogenase is only NAD specific. Since the specific activity of 3-PGAL generated from glucose is converted mainly into pyruvate which is channeled into the TCA cycle. All enzymes of the TCA cycle tested and NADH oxidase are detected in the cells of T.concretivorus grown in thiosulfate. The specific activities of fumarase and isocitrate dehydrogenase are high and others are low. The presence of two isocitrate dehydrogenase (NAD-and NADP-linked) may have important regulatory function for this organism. The activity of NAD-oxidase, which is implicated in the energy generating metabolism, was very high in the crude cell-free extract of T.concretivorus, recording 55.11 m.mu. mole/min/mg protein. This well coincides with the fact that activities of NAD-linked G-6-P dehydrogenase, 6-PG dehydrogenase and 3-PGAL dehydrogenase were high.

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Thiobacillus concretivorus의 대사경로에 관한 효소학적 연구 (The enzymatic Studies on Metabolic Pathways in Thiobacillus conctetivorus)

  • 하영칠
    • 미생물학회지
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    • 제11권1호
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    • pp.1-18
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    • 1973
  • A study was made on enzymes of carbohydrate metabolism in T. concretivorus grown with and without glucose. The present results show that T. concretivorus possesses high activities of pentose shunt pathway and related enzymes, glucokinase, G-6-P dehydrogenase, 6-PG dehydrogenase, and phosphoglucoisomerase, but low activities of enzymes unique to EMP(fructose-1, 6-diphosphate aldolase). Although the synthesis of the latter enzymes remains largely unaffected by the growth enviroment, that of the former is stimulated by glucose. And the failure to detect ED pathway enzymes in cells grown in thiosulate or thiosulfate-glucose medium eliminates the ED pathway as a significant route of glucose catabolism in T.concretivorus. These results suggest that pentose shunt pathway performs an energetic role in glucose metabolism by T.concretivorus with EMP as a subway. The absence of ED pathway and the presence of pentose shunt pathway which is the major route of catabolism in T.concretivorus are similar to those of other obligately chemolitho-trophic thiobacilli. The G-6-P and 6-PG dehydrogenase are both NAD and NADP specific, but MAD predominant. However, the 3-PGAL dehydrogenase is only NAD specific. Since the specific activity of 3-PGAL generated from glucose is converted mainly into pyruvate which is channeled into the TCA cycle. All enzymes of the TCA cycle tested and NADH oxidase are detected in the cells of T.concretivorus grown in thiosulfate. The specific activities of fumarase and isocitrate dehydrogenase are high and others are low. The presence of two isocitrate dehydrogenase (NAD-and NADP-linked) may have important regulatory function for this organism. The activity of NAD-oxidase, which is implicated in the energy generating metabolism, was very high in the crude cell-free extract of T.concretivorus, recording 55.11 m$\mu$ mole/min/mg protein. This well coincides with the fact that activities of NAD-linked G-6-P dehydrogenase, 6-PG dehydrogenase and 3-PGAL dehydrogenase were high.

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