Glucose Oxidation and It's Oxidative Enzyme Systems in Dunaliella tertiolecta. (II) Evidence for Glycolytic and Pentose Phosphate Pathways in Cell-free Extracts

Dunaliella tertiolecta의 포도당산화와 산화효소계 (II) Cell-free Extracts를 사용한 Glycolytic 및 Pentose Phosphate Pathway의 존재확인

By spectrophotometric assay method, the following enzymes could be detected in Dunaliella tertiolecta and Chlorella pyrenoidosa cell-free extracts: Hexokinase; Glucose-6-phosphate, 6-Phosphogluconate and Triosephosphate dehydrogenase; Transketolase; Phosphogluco and Ribosephosphate isomerase; Phosphoglucomutase; Phosphofructokinase; Fructosediphosphate aldorase and Ribulosephosphate 3-epimerase. Such enzymes are in accordance with the proposed pathway of glucose catabolism by D. tertiolecta as well as C. pyrenoidosa. Also, it could be estimated, under the presence of NADP, that pentose phosphate pathway were more active than glycolytic pathway in D. tertiolecta cell-free systems.