• 한국동물학회지
• /
• 제8권2호
• /
• pp.37-41
• /
• 1965

The frequency and pattern of sex chromatin in primary cultured cells of kidney cortex of cats and guinea-pigs, and muscle of chick embroys were examined and compared to those of in vivo condition, with special reference to the various cultured stages. 1. In cat, the frequencies of sex chromatin positive I of peripheral position were average 62.7% in female, and 15% in male, whereas those of non-peripherla position were 5.8% in female and 0.1% in male. The incident proportion between them showed a marked difference-approximately 10 times higher in female than male. These results failry indicated that a distinct nuclear dimorphism with regard to the sex chromatin positive I was established in cultured cells. The position of sex chormatin was usually peripheral location. The tendency of frequencies , with reference to the cultured stages, was low count in primary extracted and initial stage cells , but it showed a peaked frequency in 10-13 days after primary culture, and after that the frequencies were decreased gradually. Compared between I vitro and in vivo condition of the same tissues, the cells in vivo exhibited the sex chromatin in high frequency at the peak showed stage. 2. In guinea-pig , the frequencyies of peripheral positive I were 36.8% in female and 6.3% in male, while non-peripheral positions were 6.1% in female and 3.5% inmale. Its incident was a rate of nearly 4 times higher in female than male. The nuclear dimorphism was also established in cultrued cells of guinea-pig. The position and the incident frquency showed a similar pattern as in cat except the primary extracted cells. 3. In chick embryo, the frequencies of sex chromatin positive I of peripheral position were 38.2% in female, and 18.3% in male, non-peripheral position, however, was hardly to find. These results suggest that the definite sexual dimorphism was unable to find in chick embryo cultured cells. The position and the incident tendency were a similar pattern as in above mammals and the frequency was higher in vitro cells.

• 한국임상수의학회지
• /
• 제26권3호
• /
• pp.200-204
• /
• 2009

This study was performed to evaluate the effects of reduced L-glutathione on the oxidant/antioxidant status(superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), protein carbonyl and lipid hydroperoxide(LPO) concentration), renal function(blood urea nitrogen(BUN) and serum creatinine levels), and microscopy of renal tissues in pigs undergoing unilateral renal ischemia-reperfusion(I/R). Sixteen Landrace and Yorkshire mixed-breed pigs were divided randomly into two groups: untreated control group and reduced L-glutathione-treated group(4 mg/kg IV). Each group had 8 pigs. Pigs were unilaterally nephrectomized and the kidney was subject to 30 min of renal pedicle occlusion. Blood samples for biochemical assay were collected on days 1, 3, 5, 7, and 14 post nephrectomy. Renal I/R injury were evaluated histopathologically by the microscopic observation of renal tissue sections and biochemically by the measurement of the plasma creatinine and urea levels. Parameters of oxidative stress such as SOD, GPx, CAT, protein carbonyl and LPO were measured. The elevation of creatine and BUN levels was lower in the treated group, compared with the control group. The activities of antioxidant-enzyme were higher in the treated group, compared with the control group. In histological findings, the severity of damage in the reduced L-glutathione treated group was less when compared to the control group.

• Journal of Nutrition and Health
• /
• 제52권4호
• /
• pp.408-411
• /
• 2019

Purpose: This study investigated the effect of bioactive Yeonsan Ogye peptides (YOPs) intake on changes in the hepatic anti-oxidant indexes in male rats. Methods: Sprague-Dawley male rats were divided into 3 groups and given a casein-based AIN-93G diet and distilled water ad libitum without any added YOPs (control), distilled water with 250 mg of YOPs (Y250), or 500 mg of YOPs (Y500) per kg of body weight for 4 weeks. YOP dose was decided as referred to in the referenced study where toxicity did not occur. The hepatic anti-oxidant indexes were determined using a commercial kit. Statistical analysis was performed using SPSS version 23.0 and are expressed as $mean{\pm}standard$ error of mean. Differences among the groups were evaluated by one-way analysis of variance followed by post hoc Duncan's multiple comparisons test. Results: There were no differences in the body weights, weight gain, food intake, food efficiency ratio, or organ weight, including liver, kidney, spleen, thymus, and epididymal fat, among all of the groups. The hepatic nitric oxide (NO) level in the Y500 group was lower than that in the control and Y250 groups, and the hepatic malondialdehyde (MDA) level was lower in the Y500 group than in the Y250 group. The differences in hepatic superoxide dismutase (SOD) and catalase (CAT) activities were not statistically significant between the groups. From these results we speculated that YOPs may have anti-oxidative abilities to regulate NO and MDA production without affecting SOD and CAT activities. Conclusion: YOPs are presumed to act as anti-oxidants in the animal or human body.

• 대한수의학회지
• /
• 제62권1호
• /
• pp.10.1-10.9
• /
• 2022

Feline panleukopenia virus (FPV) causes fatal leukopenia and severe hemorrhagic diarrhea in cats. Although FPV isolates have been reported worldwide from several animals, the biological and genetic features of South Korean FPVs remain unclear. We characterized molecularly South Korean FPV isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate FPV from 60 organ homogenates. The isolates were confirmed to be FPVs via analyses of cytopathic effects, immunofluorescence studies, electron microscopy, and polymerase chain reaction. Viral genetic analyses used the full VP2 sequences. Eight isolates propagated in CRFK cells were confirmed to be FPVs. All isolates yielded viral titers ranging from 10^4.5 to 10^6.0 TCID₅₀/mL 5 days after inoculation into CRFK cells and exhibited hemagglutination titers ranging from 2⁷ to 2¹² (using pig erythrocytes). The Korean FPV isolates grew well in cat cells such as CRFK and Fcwf-4 cells. The FPV isolates were most similar to the KS42 strain isolated from a Korean cat in 2008. The FPV isolates will serve as useful antigens in future sero-epidemiological studies and will aid in the development of diagnostic tools.

• BMB Reports
• /
• 제40권3호
• /
• pp.382-395
• /
• 2007

The herb, Cajanus indicus L, is well known for its hepatoprotective action. A 43 kD protein has been isolated, purified and partially sequenced from the leaves of this herb. A number of in vivo and in vitro studies carried out in our laboratory suggest that this protein might be a major component responsible for the hepatoprotective action of the herb. Our successive studies have been designed to evaluate the potential efficacy of this protein in protecting the hepatic as well as renal tissues from the sodium fluoride (NaF) induced oxidative stress. The experimental groups of mice were exposed to NaF at a dose of 600 ppm through drinking water for one week. This exposure significantly altered the activities of the antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione reductase (GR) and the cellular metabolites such as reduced glutathione (GSH), oxidized glutathione (GSSG), total thiols, lipid peroxidation end products in liver and kidney compared to the normal mice. Intraperitoneal administration of the protein at a dose of 2 mg/kg body weight for seven days followed by NaF treatment (600 ppm for next seven days) normalized the activities of the hepato-renal antioxidant enzymes, the level of cellular metabolites and lipid peroxidation end products. Post treatment with the protein for four days showed that it could help recovering the damages after NaF administration. Time-course study suggests that the protein could stimulate the recovery of both the organs faster than natural process. Effects of a known antioxidant, vitamin E, and a non-relevant protein, bovine serum albumin (BSA) have been included in the study to validate the experimental data. Combining all, result suggests that NaF could induce severe oxidative stress both in the liver and kidney tissues in mice and the protein possessed the ability to attenuate that hepato-renal toxic effect of NaF probably via its antioxidant activity.

• Parasites, Hosts and Diseases
• /
• 제41권2호
• /
• pp.107-111
• /
• 2003

The surface ultrastructure of Acanthotrema felis (Trematoda: Heterophyidae) adults, recovered from a kitten experimentally infected with the metacercariae, was observed using a scanning electron microscope. The worm was leaf-like, ventrally concave and covered with scale-like multi-pointed tegumental spines. The spines on the anterior surface were short but broad, and had 10-12 pointed tips. The cytoplasmic processes protruded around the spines, like pockets for the spines. The ventrogenital opening was crescent, or kidney-shaped, and had protuberances with minute spines on its surrounding tegument. The spines on the posterior surface were long, but narrow, with 6-8 pointed tips. The cytoplasmic processes on this tegument were ridge-like, and elevated along the row of the spines. The surface ultrastructure of A. felis is generally similar to that of other heterophyid flukes, but some features are characteristic, and may be of taxonomic and bio-ecological significance.

• Advances in Traditional Medicine
• /
• 제4권1호
• /
• pp.22-27
• /
• 2004

Cisplatin is a highly effective and extensively used anticancer drug. Higher doses of cisplatin manifest acute nephrotoxicity and this is one of the limiting factors of this drug in cancer chemotherapy. The effect of the oyster mushroom extract to ameliorate cisplatin ( cis platinum (II) diammine dichloride) induced nephrotoxicity and restoration of antioxidant defence system in mice was investigated. The investigations showed that prior administration of methanolic extract of Pleurotus florida at a dose of 500 and 1000mg/Kg body weight significantly reduced elevated serum creatinine and urea levels and increased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in the kidney, consequent to cisplatin treatment, in a dose dependent manner. The extract restored the decreased reduced glutathione (GSH) activity and increased malondialdehyde (MDA) level due to cisplatin administration. The results thus indicated that oyster mushroom extract rendered significant protection against cisplatin induced nephrotoxicity and depletion of antioxidant defence system in a dose dependent manner. Since oyster mushrooms are excellently edible and non-toxic, the finding reported here is of significant use in cancer chemotherapy.

• 생명과학회지
• /
• 제26권7호
• /
• pp.772-781
• /
• 2016

쇠똥구리는 동물의 분을 이용하여 토질을 개선시켜 지구 생태계에 매우 중요한 역할을 담당하는 곤충이다. 이 연구에서 수컷 SD rat는 5군으로 분리되어 PBS, 쇠똥구리 에탄올 추출물, 쇠똥구리 아세톤 추출물, 귀뚜라미 아세톤 추출물, 양성대조군으로 새우기름 그룹으로 나누었다. 13주령 쥐에 고지방식이로 비만을 7주간 유도하고, 20주령부터 시험물질을 고지방 식이를 하면서 한 달간 투여하였다. 쇠똥구리 추출물의 투여는 체중과 복부지방과 부고환지방 중량의 감소로 이어졌다. 지질의 산화적 스트레스는 간에서 MDA를 측정하여 평가하였으나, 군간에 유 의성이 없었다. 단백질의 산화적 스트레스는 혈액에서 단백질 카르보닐 양으로 측정되었는데 쇠똥구리 에탄올 추출물과 쇠똥구리 아세톤 추출물에서 유의성있게 산화적 스트레스가 감소되었다. 반면에 간에서의 단백질 카르보닐 양은 군간에 유의성이 없었다. 당뇨 혈관내피세포를 효소면역분석법을 사용하여 세포부착단백질인 laminin 과 fibronectin 수준을 측정하였더니 쇠똥구리 추출물에서 유의성이 있었다. 사이토카인 IL-10, IL-1β, VEGF, eNOS를 측정하였는데 IL-10에서 쇠똥구리 에탄올 추출물과 쇠똥구리 아세톤 추출물에서 유의성이 있었다. 항산 화효소인 SOD, GSH-Px 활성은 군간에 유의성은 없었지만, 증가하는 경향이 있었고, CAT활성은 쇠똥구리 추출물에서 유의성있게 증가되었다. 지방조직 내의 포화지방산 비율이 감소하는 경향이 나타났으며, 불포화지방산과 다 가불포화지방산은 증가하였다. 쇠똥구리 추출물의 지방조직 감소 및 여러 지질 수치의 향상효과는 쇠똥구리 추출물을 기능성 곤충 소재로 이용할 수 있는 가능성을 제시한다.

• 한국임상수의학회지
• /
• 제41권2호
• /
• pp.71-78
• /
• 2024

Changes in elimination behavior, including urination and defecation, are common clinical signs of numerous disorders in cats. Therefore, this study attempted to automatically measure the elimination behavior of cats using the litter box and develop an early warning system for the guardian in case of abnormalities. To construct an early warning system for abnormal changes through cat elimination behavior, it consisted of a litter box, an automatic sensor for data collection and data wifi transmission, a server for data analysis, and a mobile phone app for result transmission and early warning. To establish the reference interval (RI), the elimination behavior was monitored for more than 2 weeks using a motion sensor within a litter box in 37 healthy cats and 19 diseased cats. The data were expressed as daily total visits, daily total stay duration, average stay duration per elimination, weekly total visits, and weekly total stay duration. Healthy cats showed median daily total visits of 3 times/day (RI 1.0-7.0) and daily total stay duration of 192 s/day (RI 8.0-452.0). For weekly data, the median total visits were 20 times/week (RI 3.0-35.25) and the median total stay duration was 1,147 s/week (RI 80.0-2,249.5). The average stay duration per elimination was 59 s/elimination (RI 4.67-132.0). Diseased cats showed more frequent elimination behavior than healthy cats (p < 0.001). Otherwise, for each elimination, diseased cats had shorter stay durations than healthy cats (p < 0.001). This study established the RIs of elimination behavior parameters (frequency and duration) in healthy cats. The present study might help guardians and veterinarians detect changes in elimination behaviors in diseased cats at an early stage.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권5호
• /
• pp.686-690
• /
• 2015

The adrenergic receptor beta 2 (ADRB2) plays a role in various physiological responses of the muscle to exercise, such as contraction and relaxation. Given its important role in muscle function, we investigated the structure of the horse ADRB2 gene and its expression pattern after exercise to determine if it can serve as a putative biomarker for recovery. Evolutionary analyses using synonymous and non-synonymous mutation ratios, were compared with other species (human, chimpanzee, mouse, rat, cow, pig, chicken, dog, and cat), and revealed the occurrence of positive selection in the horse ADRB2 gene. In addition, expression analyses by quantitative polymerase chain reaction exhibited ubiquitous distribution of horse ADRB2 in various tissues including lung, skeletal muscle, kidney, thyroid, appendix, colon, spinal cord and heart, with the highest expression observed in the lung. The expression of ADRB2 in skeletal muscle was significantly up-regulated about four folds 30 minutes post-exercise compared to pre-exercise. The expression level of ADRB2 in leukocytes, which could be collected with convenience compared with other tissues in horse, increased until 60 min after exercise but decreased afterward until 120 min, suggesting the ADRB2 expression levels in leukocytes could be a useful biomarker to check the early recovery status of horse after exercise. In conclusion, we identified horse ADRB2 gene and analyzed expression profiles in various tissues. Additionally, analysis of ADBR2 gene expression in leukocytes could be a useful biomarker useful for evaluation of early recovery status after exercise in racing horses.