• 한국태양에너지학회 논문집
• /
• 제32권6호
• /
• pp.141-148
• /
• 2012

Ceiling cassette type air conditioner has been a main stream as a heating/cooling system recently in school, Korea. In this study, indoor thermal environments made by ceiling cassette type air conditioner were investigated by CFD simulation. Concentrations of $CO_2$ were investigated by a field measurement. Indoor thermal environment with the velocity inlet angle of $45^{\circ}$ from the ceiling in heating season was very ununiform so that thermal area was divided into two parts those the one is window side which is cold, and the other is corridor side which is hot. In cooling season under the same condition, there are areas too hot or too cold. If the velocity inlet angle is set in $30^{\circ}$ from the ceiling, indoor thermal environments was improved greatly in cooling season and heating season, too. Also, from the field measurement of $CO_2$ concentrations, it was suggested to install ventilators with proper air volume considered the number of class students.

• Parasites, Hosts and Diseases
• /
• 제53권3호
• /
• pp.335-339
• /
• 2015

Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of $Ca^{2+}$, $Mg^{2+}$, $K^+$, and $HCO_3{^-}$ in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.

• Journal of Microbiology and Biotechnology
• /
• 제24권7호
• /
• pp.1008-1013
• /
• 2014

The use of antimicrobial agents for additives or therapeutics is strongly associated with a prevalence of antimicrobial resistance in commensal Enterobacteriaceae. We aimed to characterize integrons in Enterobacteriaceae isolates obtained from chicken cecums in Korea. Moreover, the correlation between integron gene cassettes and antimicrobial resistance was also investigated. A total of 90 isolates the belonged to Enterobacteriaceae were recovered from chickens grown at Gyeongsang and Chungcheong provinces in Korea. Antimicrobial susceptibility tests were performed by the disk diffusion method. PCR and DNA sequencing were also performed to characterize the gene cassette arrays of the integrons. Of the 90 Enterobacteriaceae isolates tested, 39 (43.3%) and 10 (11.1%) isolates carried class 1 and 2 integrons, respectively. Whereas the class 2 integron did not contain gene cassettes, the class 1 integrons carried seven different gene cassette arrays. The class 1 integrons harbored genes encoding resistant determinants to aminoglycosides (aadA1, aadA2, and aadA5), trimethoprim (dfrA1, dfrA12, dfrA17, and dfrA32), lincosamides (linF), and erythromycin (ereA). Moreover, the presence of a class 1 integron was significantly related to a high resistance rate of antimicrobial agents, such as spectinomycin and trimethoprim. We confirmed that diverse class 1 integrons were widely distributed in Enterobacteriaceae isolates from chickens and directly contributed to the resistance to diverse antimicrobial agents in Korea.

• 대한전자공학회논문지
• /
• 제12권3호
• /
• pp.1-7
• /
• 1975

이 논문은 음성카셋트 Deck을 이용하여 고밀도로 data를 기록 또는 재생하는 한 방법을 검토하였다. 기록에 있어서는 NRZ-TM의 혼합에 의한 한 방식으로서 data를 정 및 부 bit로 분리하여 두개의 트랙에 각각 기록한다. 재생방법은 피이크. 쎈스에 의하여 검출신호의 레벨변동에 의한 정형, 펄스의 시간편차를 제거하고 오차의 정정이 자동적으로 이루어 지며 크럭. 펄스가 검출신호 자체에서 자동적으로 발생한다. 이 방식은 음성카셋트 Deck의 내부회로는 변경하기 않고 외부에 새로운 부착만으로서 음성과 data를 병용할 수 있다. Data펄스 25Hz-4KHz의 주파수까지 기록이 가능하고 최고 전송속도는 787(bps)이다.

• 대한설비공학회:학술대회논문집
• /
• 대한설비공학회 2006년도 하계학술발표대회 논문집
• /
• pp.1180-1185
• /
• 2006

Modern people spend most of time at indoor space, such as office or classroom. Especially, occupants are exposed to the airtight indoor air quality (IAQ) for a long time, At present, many studies on the air-conditioning systems are more focused on the individual thermal comfort than the thermal efficiency due to increase of the concern of health. There are several factors which are influenced thermal comfort, such as temperature, humidity, convection and air movement, etc. Also, the individual factor, such as age, gender, Physical constitution and habit, should be considered. The 4-way cassette type air conditioner is known to bring out better performance about thermal comfort than the traditional one. This study is performed on the higher ceiling environment than the common buildings or classrooms. Also, this study analyzed on the Indoor thermal comfort by diffusing direction of 4-way cassette air conditioner with various discharge angles, $45^{\circ},\;50^{\circ},\;55^{\circ}$ and $60^{\circ}$. Using a commercial code, FLUENT, three-dimensional transient air thermal flow fields are calculated with appropriate wall boundary conditions and standard $k-{\epsilon}$ turbulence model. Results of velocity and temperature distributions are graphically depicted with various discharge angles.

• 방사선산업학회지
• /
• 제11권1호
• /
• pp.1-6
• /
• 2017

Reported some level of bacteria in areas that are well made contact in Radiology imaging room evaluate the importance of cleanliness in the hospital management of equipment to check for the presence of pathogenic bacteria. Gwang-ju and Jeol-la city and medium-sized hospitals in the material with a cotton swab and rub evenly Radiology selection cassette, a handle, Apron of the imaging apparatus having the most contact with patients from July 2016 to August 2016 as a target in place and special studios 6, and saline solution will placed in a test tube containing. The swab sample was diluted 1,000 times, you can see the bacteria and the intestinal bacterial selective medium Trypticase Soy Agar (TSA), Muller-Hinton Agar (MHA), Eosin-Methylene Blue (EMB), ENDO(BD, NJ, USA) then incubated smear to. In the incubator (incubator, SANYO, Japan) was observed after incubation of bacteria and counting the total number of bacteria also Colonies (colony) suspected intestinal bacteria were isolated and cultured on KIA medium (BD, NJ, USA). As a result, it was found that this came Gram positive Coccus A hospital handle the F hospital, from the C Gram positive Coccus cassette and handle the F hospital. The striking yellow coloring Staphylococcus aureus 110 agar (STA 110) in the medium sample, but it is suspected staphylococcal Coccus to the final identification in the laboratory is not a single specimen of the two samples from Gram positive Coccus biochemical identification Identification Kit is an API could not, it was thought to be non-Staphylococcus aureus was cultured on blood agar suggesting that (BAP) blood of dance. Dynamic tests were conducted biochemical API kit of the two samples were identified from Gram positive Coccus bacteria Escherichia coli (E. coli) is F hospital cassette was confirmed Eenterobacter cloaca in A hospital possession. Did not aggregate O-26, O-111, O-157 and the serum test was conducted in the laboratory from the E. coli F cassette hospital.

• 생명과학회지
• /
• 제28권11호
• /
• pp.1277-1284
• /
• 2018

본 연구에서는 효모염색체내에 다양한 유전자 발현 cassette를 도입하기 위해 Cre/loxP system을 가진 repeated yeast integrative plasmid (R-YIp)를 구축하였다. R-YIp는 반복적으로 형질전환체를 선별할 수 있는 selective marker (CgTRP1)와 loxP 서열, 그리고 integration을 위한 목적서열을 함유하고 있어 같은 염색체의 동일한 위치에 여러 개의 유전자 발현 cassette를 도입하는 것이 가능하다. 따라서 xylan/xylose 대사에 관련된 endoxylanase (XYLP), ${\beta}$-xylosidase (XYLB), xylose reductase (GRE3) 그리고xylitol dehydrogenase (XYL2)의 효모염색체내에 도입을 시도하였다. 먼저 XYLP, XYLB, GRE3그리고 XYL2 유전자의 효율적인 발현을 위한 promoter를 선별하기 위해 pGMF-GENE과 pAMF-GENE plasmid를 구축하였고, 각 유전자들의 발현에 GAL10 promoter가 적합함을 확인하였다. 다음으로 GAL10p-GENE-GAL7t cassette를 가진 pRS-GENE plasmid (R-YIp)를 구축하여, 반복적 integration 과정과 selective marker의 제거를 통해 각각의 R-YIps를 효모 7번염색체에 순차적으로 도입하였다. R-YIp system을 통해 효모염색체내에 도입된 유전자들은 모두 안정적으로 발현되었고, 활성형의 재조합효소를 생산함을 확인할 수 있었다. 따라서 다수의 외래유전자를 효모염색체내 도입함에 있어 selective marker와 숙주세포 선택의 한계를 R-YIp system을 통해 어느 정도 극복할 수 있을 것이라 기대한다.

• 월간포장계
• /
• 통권107호
• /
• pp.137-145
• /
• 2002

• 대한방사선과학회:학술대회논문집
• /
• 대한방사선과학회 1993년도 학술대회
• /
• pp.18-19
• /
• 1993

• 대한방사선과학회:학술대회논문집
• /
• 대한방사선과학회 1993년도 학술대회
• /
• pp.19-20
• /
• 1993