• Applied Biological Chemistry
• /
• v.18 no.2
• /
• pp.56-60
• /
• 1975

A rapid method for the measurement of the absolute activity of carbon-14 in cotyledons and root of etiolated pea seedlings has been developed. Fresh tissue was frozen in liquid air, ground and suspended in gel phosphor and subjected to measurement for its radioactivity by liquid scintillation counter. Apparent activity of the suspended tissue sample calculated by counting efficiency value obtained by internal standardisation, was found to be related to absolute activity of the tissue, determined by flask combustion technique, by a constant factor. Once this factor is determined experimentally, analysis of C-14 lebelled tissue involves only fairly simple suspension counting by liquid scintillation counter. Present method appears to be applicable to other tissues tagged with C-14.

• Tuberculosis and Respiratory Diseases
• /
• v.38 no.1
• /
• pp.45-52
• /
• 1991

Clinical analysis was performed on 306 patients with cervical lymphadenopathy who were diagnosed histologically by fine needle aspiration biopsy cytology (FNABC) and/or excisional biopsy from Jan 1986 to Jan 1990 at Hanyang University hospital. The results obtained were as follows: 1) Of 306 patients with cervical lymphadenopathy, 216 (70.6%) were inflammatory lesions, and 90 (29.4%) malignant lesions. Tuberculous lymphadenitis of inflammatory lesions was most common (134 cass: 62%). Of malignant lesions, metastatic cancer was more frequent (75 cases: 83.3%). 2) The sex ratio were as follows: inflammatory lesion; M:F=1 : 1.8 (tuberculous lymphadenitis;M : F=1:2.3) malignant lesion; M : F=1.5 : 1 (metastatic cancer; M : F=2.6 : 1) 3) The peak age of inflammatory lymphadenopathy was 20-29 years old (38.9%), and that of malignant lesion 50-59 years old (46.7%). 4) In more than half of tuberculous lymphadenitis and metastatic cancer, the location of enlarged lymph nodes was one side of the neck and the number was more than one. 5) The common primary sites of metastatic cancer were lung and stomach. In 11 cases (14.7%), the primary site could not be found. 6) The sensitivity and the specificity of fine needle aspiration biopsy cytology (FNABC) was 0.83 & 1.0 in metastatic cancer respectively.

• Proceedings of the KSME Conference
• /
• 2007.05a
• /
• pp.17-22
• /
• 2007

Low-cycle environmental fatigue tests of cast austenitic stainless steel CF8M at the condition of fatigue strain rate 0.04%/sec were conducted at the pressure and temperature, 15MPa, $315^{\circ}C$ of a operating pressurized water reactor. The used test rig was limited to install an extensometer at the gauge length of the cylindrical fatigue specimen inside the small autoclave. So the magnet type LVDT's were used to measure the fatigue displacement at the specimen shoulders inside the high temperature and high pressure water autoclave. However, the displacement and strain measured at the specimen shoulders is different from the one at the gauge length for the geometry and the cyclic strain hardening effect. FEM calculated the displacement and the strain of the gauge length from the data measured at the shoulders. Tensile test properties in elastic and plastic behavior of CF8M material were used in the FEM analysis. A series of low cycle fatigue tests simulating the cyclic strain hardening effect verified that the FEM calculation was well agreed with the simulated tests. The process and method developed in this study would be so useful to produce reliable environmental fatigue curves of CF8M stainless steel in pressurized water reactors.

• The Plant Pathology Journal
• /
• v.28 no.2
• /
• pp.149-155
• /
• 2012

A field trial was conducted in 2009/2010 and 2010/2011 growing seasons at Giza Agricultural Research Station to examine correlations between some biochemical componets and powdery mildews ($PM_s$) resistance in flax cultivars. Nine flax cultivars could be divided into five distinct groups, i.e., highly susceptible (Cortland and C.I. 2008), moderately susceptible (Giza 7, and Marshall), moderately resistant (Cass), resistant (Koto, Dakota and Wilden), and highly resistant (Ottowa 770B). The cultivars showed considerable variation in PM severity ranged from 8.05 on Ottowa 770B to 97.02% on Cortland. Total soluble proteins, total phenols, antioxidant enzymes (peroxidase and polyphenoloxidase), ascorbic acid, tocopherol, and malondialdehyde (MDA), were determined in uninfected leaves of the tested cultivars. Pearson's correlation coefficient was calculated to measure the degree of association between PM severity and each component. All components showed significant (P < 0.05) or highly significant (P < 0.01) negative correlation with PM severity except MDA, which showed positive correlation (P < 0.01). Linear regression analysis was used to evaluate the causal relationship between the biochemical components (independent variables) and PM severity (dependent variable). Coefficient of determination ($R^2$) values of the generated models ranged from 48.76 to 77.15%. Tocopherol, MDA, and proteins were the most important contributors to the total variation in PM severity as the $R^2$ values of their models were 71.78, 75.28, and 77.15%, respectively. The results of the present study suggest that tocopherol, MDA, and proteins in uninfected leaves can be used as biochemical markers to predict PM resistance in flax.

• Journal of dental hygiene science
• /
• v.21 no.4
• /
• pp.251-259
• /
• 2021

Background: The purpose of this study was to investigate the effects of commercially available calamansi soju and other alcoholic beverages on the microhardness and erosion of resin restorations. Methods: In this study, we evaluated the effects of Calamansi soju, Chamisul fresh, Cass fresh, and Gancia Moscato D'asti on resin restorations. Jeju Samdasoo and Coca-Cola were used as negative and positive controls, respectively. Specimens to be immersed in the beverages were manufactured using composite resin according to the product instructions. In each group, the surface microhardness was measured using a surface microhardness instrument before and after immersion for 5, 15, 30, and 60 minutes. The pattern of change in the surface of the composite resin was observed under a scanning electron microscope (SEM). Paired t-tests, one-way ANOVA, and repeated measures ANOVA were performed to compare the surface microhardness of the specimens, and the Tukey test was used as a post hoc test. Results: The pH of all beverages except Jeju Samdasoo was <5.5, which is the critical pH that can induce erosion. The difference in surface microhardness of the composite resin before and after immersion for 60 minutes was significant in all groups. In particular, the largest change in surface microhardness was observed in the calamansi soju group. In the SEM analysis, loss of composite resin was observed in all groups except the Jeju Samdasoo group, and rough surfaces with pores of various sizes were observed. Conclusion: In this study, all beverages except Jeju Samdasoo decreased the microhardness of the composite resin surface, and it was confirmed that calamansi soju had the greatest change.

• BMB Reports
• /
• v.39 no.1
• /
• pp.68-75
• /
• 2006

In higher plants, P450s participate in the biosynthesis of many important secondary metabolites. Here we reported for the first time the isolation of a new cytochrome P450 cDNA that expressed in a stem-specific manner from Camptotheca acuminata (designated as CaSS), a native medicinal plant species in China, using RACE-PCR. The full-length cDNA of CaSS was 1735 bp long containing a 1530 bp open reading frame (ORF) encoding a polypeptide of 509 amino acids. Bioinformatic analysis revealed that CASS contained a heme-binding domain PFGXGRRXCX and showed homology to other plant cytochrome P450 monooxygenases and hydroxylases. Southern blotting analysis revealed that there was only one copy of the CaSS present in the genome of Camptotheca acuminata. Northern blotting analysis revealed that CaSS expressed, in a tissue-specific manner, highly in stem and lowly in root, leaf and flower. Our study suggests that CaSS is likely to be involved in the phenylpropanoid pathway.

• The Journal of the Korea Contents Association
• /
• v.21 no.5
• /
• pp.276-284
• /
• 2021

The purpose of this study is to analyze differences in satisfaction with remote learning after the outbreak of COVID-19 between two-year college students and four-year university students. The analysis results regarding differences in satisfaction according to the types of remote learning were as follows: first, male students had greater satisfaction with the hybrid type of in-person and non-contact lecturers than female students; secondly, two-year college students had a higher level of satisfaction with all the types of remote learning than four-year university students; and thirdly, there were significant differences in students' satisfaction with the hybrid type of in-person and non-contact lectures according to the grades in a four-year university. The study then analyzed differences in satisfaction with the management and content of remote learning, interactions with professors, and exams and found that two-year college students exhibited a greater level of satisfaction than four-year university students. No significant differences were found in satisfaction with remote learning according to the grades both in two-year college students and four-year university students. The findings of the study demonstrate significant differences in overall satisfaction with remote-learning according to college and university types. That is, two-year college students had greater satisfaction with remote learning than four-year university students. It is critical to take into consideration the characteristics of students according to college and university types in the development process in order to raise the effects of remote learning based on these analysis results.