• 대한기계학회논문집B
• /
• 제25권3호
• /
• pp.442-450
• /
• 2001

Experiments were carried out on agglomeration of bipolarly charged particles in an alternating current electric field. Laboratory-scale setup was built and experiments were conducted at atmospheric condition. DOS(Di-octyl Sebacate) particles with 100% purity were generated by an atomizer. The particles were branched into two, each of which passed through a wire-to-plate type charging section where a positive or a negative DC high voltage was applied and was charged positively or negatively. These bipolarly charged particles together passed through an agglomeration section where an $\pm$20kV AC power was applied between two plates. The resident time in the agglomeration section was adjusted as l sec. Particle sampling was made by a cascade impactor (MOUDI). The effect of agglomeration system on the reduction ratio of particles below l ㎛ was 42∼45%. Effect of AC frequency on the particle size distribution was found insignificant.

• Bulletin of the Korean Chemical Society
• /
• 제15권8호
• /
• pp.663-669
• /
• 1994

Decay kinetics of Ga(5s), Ga(5p) and Ga(4d) atoms in Ar were studied by laser induced fluorescence technique. Theground state gallium atoms in the gas phase were generated by pulsed dc discharge of trimethyl gallium and argon mixtures. Both pulsed discharge and YAG-DYE laser system were controlled by a dual channel pulse generator and the delay time between the end of discharge and laser pulses was set 3.0-6.0 ms. The Ga(5s) and Ga(4d) atoms were generated by single photon excitation from the ground state Ga atoms and radiative lifetimes as well as the total quenching rate constants in Ar were obtained from the pressure dependence of the fluorescence decay rates. The Ga(5p) atoms were populated by a two-photon excitation method and the cascade fluorescence from Ga(5s) atoms were analyzed to extract quenching rate constant of Ga(5p) atoms by Ar in addition to radiative lifetimes of Ga(5p) state. The magnitudes of the quenching rate constants by Ar for the low-lying excited states of Ga atoms are 1.6-3$ {\times}10^{-11}cm^3$ molecul$e^{-1}s^{-1}$, which are much larger than those for alkali, alkaline earth and Group 12 metals. Based on the measured rate constants, kinetic simulations were done to assign state-to-state rate constants.

• Parasites, Hosts and Diseases
• /
• 제61권4호
• /
• pp.428-438
• /
• 2023

Clonorchis sinensis is commonly found in East Asian countries. Clonorchiasis is prevalent in these countries and can lead to various clinical symptoms. In this study, we used overlap extension polymerase chain reaction (PCR) and the Xenopus laevis oocyte expression system to isolate a cDNA encoding the choline transporter of C. sinensis (CsChT). We subsequently characterized recombinant CsChT. Expression of CsChT in X. laevis oocytes enabled efficient transport of radiolabeled choline, with no detectable uptake of arginine, α-ketoglutarate, p-aminohippurate, taurocholate, and estrone sulfate. Influx and efflux experiments showed that CsChT-mediated choline uptake was time- and sodium-dependent, with no exchange properties. Concentration-dependent analyses of revealed saturable kinetics consistent with the Michaelis-Menten equation, while nonlinear regression analyses revealed a Km value of 8.3 µM and a Vmax of 61.0 pmol/oocyte/h. These findings contribute to widen our understanding of CsChT transport properties and the cascade of choline metabolisms within C. sinensis.

• Anatomy and Cell Biology
• /
• 제56권4호
• /
• pp.421-427
• /
• 2023

Bladder exstrophy is a rare congenital condition of the pelvis, bladder, and lower abdomen that opens the bladder against the abdominal wall, produces aberrant growth, short penis, upward curvature during erection, wide penis, and undescended testes. Exstrophy affects 1/30,000 newborns. The bladder opens against the abdominal wall in bladder exstrophy, a rare genitourinary condition. This study is vital to provide appropriate therapy choices as a basis to improve patient outcomes. This study may explain bladder exstrophy and provide treatment. Epispadias, secretory placenta, cloacal exstrophy, and other embryonic abnormalities comprise the exstrophy-spades complex. The mesenchymal layer does not migrate from the ectoderm and endoderm layers in the first trimester, affecting the cloacal membrane. Embryological problems define the exstrophy-aspidistra complex, which resembles epimedium, classic bladder, cloacal exstrophy, and other diseases. Urogenital ventral body wall anomalies expose the bladder mucosa, causing bladder exstrophy. Genetic mutations in the Hedgehog cascade pathway, Wnt signal, FGF, BMP4, Alx4, Gli3, and ISL1 cause ventral body wall closure and urinary bladder failure. External factors such as high maternal age, smoking moms, and high maternal body mass index have also been associated to bladder exstrophy. Valproic acid increases bladder exstrophy risk; chemicals and pollutants during pregnancy may increase bladder exstrophy risk. Bladder exstrophy has no identified cause despite these risk factors. Exstrophy reconstruction seals the bladder, improves bowel function, reconstructs the vaginal region, and restores urination.

• International Journal of Naval Architecture and Ocean Engineering
• /
• 제2권3호
• /
• pp.132-138
• /
• 2010

In the present study, the real-time monitoring system is developed based on the wireless sensor network (WSN) and power line communication (PLC) employed in the 3,000-ton-class training ship. The WSN consists of sensor nodes, router, gateway and middleware. The PLC is composed of power lines, modems, Ethernet gateway and phase-coupler. The basic tests show that the ship has rather good environments for the wired and wireless communications. The developed real-time monitoring system is applied to recognize the thermal environments of main-engine room and one cabin in the ship. The main-engine room has lots of heat sources and needs careful monitoring to satisfy safe operation condition or detect any human errors beforehand. The monitoring is performed in two regions near the turbocharger and cascade tank, considered as heat sources. The cabin on the second deck is selected to monitor the thermal environments because it is close to the heat source of main engine. The monitoring results of the cabin show the thermal environment is varied by the human activity. The real-time monitoring for the thermal environment would be useful for the planning of the ventilation strategy based on the traces of the human activity against inconvenient thermal environments as well as the recognizing the temperature itself in each cabin.

• 한국산업정보학회논문지
• /
• 제13권3호
• /
• pp.110-116
• /
• 2008

본 논문에서는 이진위상 컴퓨터형성홀로그램(binary phase computer generated hologram;BPCGH)과 다중 XOR 연산을 이용하여 영상의 암호화를 개선시키는 방법을 제안하고자 한다. 먼저 암호화를 위해 원영상을 재생할 수 있는 BPCGH를 반복 알고리듬을 이용하여 설계하며, 이를 암호화할 영상으로 간주하여 랜덤하게 발생시킨 위상 키 영상과의 XOR 연산을 통해 암호화한다. 암호화된 영상을 다시 XOR 연산을 통해 여러 개의 슬라이드 영상으로 나눔으로써 암호화를 개선시킨다. 홀로그램의 복호화 과정은 암호화된 슬라이드 영상과 암호화시에 사용된 무작위 위상 키 영상을 직렬 정합시킨 후, 기준파와의 간섭에 의해 수행된다. 그리고 복호화 된 홀로그램 영상은 위상 변조한 후, 역푸리에 변환하여 최종적으로 구한다. 그리고 슬라이드 영상의 패턴을 적절히 바꾸어 주면 다양한 형태의 복호화된 BPCGH 영상을 생성할 수 있다. 제안된 암호화 방법은 암호화시에 사용된 무작위 키 영상 정보가 없으면 원영상이 전혀 복원되지 않고, 암호화된 슬라이드 영상을 달리함에 따라 복원되는 홀로그램의 패턴을 다양하게 얻을 수 있으므로 차별화 된 인증 시스템에 활용할 수 있다.

• 한국산학기술학회논문지
• /
• 제13권10호
• /
• pp.4745-4750
• /
• 2012

본 논문에서는 저전압 저전력 필터 설계에 적합한 전류모드 방식을 이용하여 고속 전력선통신(PLC)모뎀 수 신단의 1MHz~30MHz 차단주파수를 갖는 6차 대역통과 필터를 설계하였다. 3차 바터워스 고역통과 필터와 3차 체비세프 저역통과 필터를 종속연결로 구성하여 대역통과 필터를 설계하였다. 필터를 구성하기 위한 핵심로써 기존의 전류미러형 적분기에 비하여 증가된 이득 및 차단주파수를 가지는 새로운 전류모드 적분기를 설계하였다. 설계된 전류모드 적분기의 이득과 차단주파수는 각각 32.2dB 및 247MHz이였다. 설계된 6차 대역통과 필터의 차단주파수는 제어전압에 따라서 200KHz에서 50MHz까지 조정이 될 수 있으며 소비전력은 공급전압 1.8V에서 2.85mW이였다. 설계된 대역통과 필터는 1.8V, $0.18{\mu}m$ CMOS 공정파라메터를 사용하여 검증되었다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제37권1호
• /
• pp.21-28
• /
• 2018

CRISPR/Cas9 gene editing system is an efficient method to mutation in a sequence specific manner. Here we report the direct transfection of the Cas9 nuclease and gene specific guide RNA can be used in BM-N cell line derived from Bombyx mori ovarian tissue to enfeeble function of endogenous gene in vitro. We have used gene editing system to negative regulation components of major signaling cascade, the Toll pathway, which controls B. mori resistance to microbe infections, such as fungi and gram positive bacteria. We demonstrate that the $I{\kappa}B-like$ protein Cactus may controls the activation of transcription factors such as Rel A and Rel B. The direct transfection of Cas9 nuclease and Cactus-specific guide-RNA complex may be used in BM-N cells to disrupt the function of endogenous genes in vitro. A mutation frequency of 30-40% was observed in the transfected cells, and various mutations caused the target region. Moreover, RT-PCR analysis revealed that Cactus gene was down regulated after these mutations. More importantly, mutation of BmCactus stimulated expression of lysozyme, moricin, and lebocin genes. These results suggest that the CRISPR/Cas9 systems are expected to efficiently induce site-specific mutations and it was possible to produce antimicrobial peptide through the gene editing.

• 한국대기환경학회지
• /
• 제8권1호
• /
• pp.20-28
• /
• 1992

The x-ray fluorescence(XRF) is one of the most convenient and widely used techniques for analyzing trace elements in ambient particulate matters. The objects of the study were to estimate the optimum exposure time using the XRF, to investigate the distributions of heavy metal levels in particulate matters, and finally to study seasonal variation for the concentrations of total suspended particulate matters(TSP) and size fractionated particulate matters. The suspended particulate matters had been collected by a cascade impactor having 9 size fragnated stages for 3 years(Dec. 1988 to Nov. 1991) in Kyung Hee University-Suwon Campus. The particulate matters were then collected on each stage by membrane filters. The weight concentrations were determined by the XRF system. Thus, seasonal variations and relationship between concentration and particle size could be investigated. Resulting distribution was bimodal with the coarse and the fine particle groups minimum occurring around 2.1 to 3.3 $\mu$m as an aerodynamic diameter. To determine optimum exposure time of the XRF for various trace inorganic elements, membrane filters and the NIST standard filters were extensively studied. Using a statistical technique, optimum exposure time was estimated for each trace element and overall elements. The time was then determined as 20 seconds for the XRF system. The concentration of TSP was 123.9$\mum/m^3$ on an arithmatic average. The levels of each inorganic metal were Si 2420.0ng/$m^3$, Fe 977.1ng/$m^3$, and so on. The Pb. Zn, and Cu abounded in the fine mode group, while Ca, Fe, Si, Al, and K in the coarse group. Marked seasonal variation of TSP and metal concentrations was observed. The concentration of heavy metals in the fine mode was highest in winter : on the other hand, that in the coarse mode was highest in spring.

• 응용통계연구
• /
• 제31권5호
• /
• pp.539-554
• /
• 2018

최근 등장하는 다양한 사물인터넷 기기 혹은 상황인식 기반의 인공지능에서는 사용자와 기기의 상호작용이 중요시 된다. 특히 인간을 대상으로 상황에 맞는 대응을 하기 위해서는 인간의 표정을 실시간으로 인식하여 빠르고 정확한 판단을 내리는 것이 필요하다. 따라서, 보다 빠르고 정확하게 표정을 인식하는 시스템을 구축하기 위해 얼굴 이미지 분석에 대한 많은 연구들이 선행되어 왔다. 본 연구에서는 웹사이트 Kaggle에서 제공한 48*48 8-bit grayscale 이미지 데이터셋을 사용하여 얼굴인식과 표정분류로 구분된 두 단계를 거치는 얼굴표정 자동 인식 시스템을 구축하였고, 이를 기존의 연구와 비교하여 자료 및 방법론의 특징을 고찰하였다. 분석 결과, Face landmark 정보에 주성분분석을 적용하여 단 30개의 주성분만으로도 빠르고 효율적인 예측모형을 얻을 수 있음이 밝혀졌다. LDA, Random forest, SVM, Bagging 중 SVM방법을 적용했을 때 가장 높은 정확도를 보이며, LDA방법을 적용하는 경우는 SVM 다음으로 높은 정확도를 보이며, 매우 빠르게 적합하고 예측하는 것이 가능하다.