• 한국진공학회:학술대회논문집
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• 한국진공학회 1998년도 제16회 학술발표회 논문개요집
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• pp.87-87
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• 1998

• 한국동물위생학회지
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• 제37권4호
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• pp.253-261
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• 2014

Porcine circovirus disease (PCVD) is a major problem of swine industry worldwide, and diagnosis of PCV2, causal agent of PCVD, has been doing in clinical laboratories of pig disease by polymerase chain reaction (PCR) methods. But the PCR analyses have a serious problem of misdiagnosis by contamination of DNA, in particular, from carryover contamination with previously amplified DNA or extracted DNA from field samples. In this study, an uracil DNA glycosylase (UNG)-based direct PCR (udPCR) without DNA extraction process and DNA carryover contamination was developed and evaluated on PCV2 culture and field pig samples. The sensitivity of the udPCR combined with dPCR and uPCR was same or better than that of the commercial PCR (cPCR) kit (Median diagnostics, Korea) on PCV2-positive serum, lymph node and lung samples of the pigs. In addition, the udPCR method confirmed to have a preventing ability of mis-amplification by contamination of pre-amplified PCV2 DNA from previous udPCR. In clinical application, 170 pig samples (86 tissues and 84 serum) were analysed by cPCR kit and resulted in 37% (63/170) of positive reaction, while the udPCR was able to detect the PCV2 DNA in 45.3% (77/170) with higher sensitivity than cPCR. In conclusion, the udPCR developed in the study is a time, labor and cost saving method for the detection of PCV2 and providing a preventing effect for DNA carryover contamination that can occurred in PCR process. Therefore, the udPCR assay could be an useful alternative method for the diagnosis of PCV2 in the swine disease diagnostic laboratories.

• 한국초지조사료학회지
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• 제37권3호
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• pp.208-215
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• 2017

This study was designed to investigate the carryover effects of high-forage to concentrate (F: C) diet in bred heifers on feed intake, feed efficiency (FE) and milk production of primiparous lactating Holstein cows. The experiment was conducted for 589 days (d) from onset of pregnancy through to the end of first lactation. Twenty-four bred heifers (Body weight: $BW=345.8{\pm}45.4kg$ and $15{\pm}1.2mon$ of age) randomly assigned to two groups of 3 pens containing 4 heifers each and fed high forage (HF) diet with F: C ratio of 91.7: 8.3% and low forage (LF) diet with F: C ratio of 77.8: 22.2% throughout the pregnancy period. After calving, lactating cows were fed total mixed ration (TMR) based diet. No differences (p > 0.05) were observed in dry matter intake (DMI) of bred heifers and primiparous lactating cows in both HF and LF groups. The FE of mid-to-late lactation period was higher (p< 0.05) in HF than LF group. However, the HF group showed higher (p < 0.05) milk yield, 4 % fat corrected milk (FCM) and energy corrected milk (ECM) than LF group during the 305 d lactation. The LF group showed higher (p < 0.05) milk fat, crude protein (CP), milk urea nitrogen (MUN), solid not fat (SNF) and somatic cell count (SCC) than HF group. It is concluded that restriction of F: C ratio to 91.7: 8.3% to bred heifers has the potential carryover effects to maintain higher milk yield and FE with no adverse effect on feed intake and milk composition of primiparous lactating Holstein cows.

• 한국융합학회논문지
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• 제13권4호
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• pp.373-381
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• 2022

2011년 3월 동일본 대지진으로 인한 후쿠시마 원전수 유출 사고는 방사능에 오염된 수산물 섭취를 통한 방사능 피폭에 대한 두려움을 높였다. 이에 따라 우리나라 정부는 후쿠시마 인근 8개 현의 농수산물 수입을 금지하였으나 일본과의 WTO 분쟁 등으로 관련 내용이 지속적으로 보도되었고 이는 수산물에 대한 소비자의 불안을 가중시킨 것으로 보인다. 본 연구는 3년간(2018년~2020년)의 수산물 일별 구매 자료를 수집하여 일본 방사능과 관련한 보도가 국내 소비자의 수산물 구매액에 미치는 영향을 다항시차분포모형을 사용하여 추정하였다. 분석 결과 방사능 관련 기사가 언론을 통해 소비자에게 노출된 후 5일째와 6일째 이틀간 통계적으로 유의하게 수산물 구매에 부정적 영향을 미친 것으로 나타났다. 관련 기사 노출 후 소비자의 인지가 수산물 구매에 반영되는 이월효과를 보여준다.

• 자원ㆍ환경경제연구
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• 제32권3호
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• pp.149-166
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• 2023

온실가스 저감을 위해 도입된 배출권거래제가 2차 계획기간(2018~2020) 중 배출권 가격 급등에 따라 원활한 배출권 수급에 대한 수요는 증가한 반면 향후 배출권 부족을 예상한 공급자들의 거래참여 부진이 발생하였다. 이로 인해 2019년 당국은 시장 안정화 조치의 일환으로 배출권의 이월량이 거래량에 비례하도록 지침을 일시적으로 개정한 바 있다. 본고는 복수참여자 동태적 비선형 수리모형을 활용한 최적화 과정을 통해 이월정책에 대한 정부의 개입이 온실가스 저감 및 배출권 시장 가격 등에 미친 영향을 분석하였다. 시뮬레이션 분석 결과, 이월제한이라는 규제가 미리 예견되는 모형의 제약에도 불구하고 규제가 실시되는 기간 중에는 확실한 매물 출하 효과로 인해 가격인하가 발생하였음을 보여주고 있다.

• 한국동물위생학회지
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• 제37권4호
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• pp.263-271
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• 2014

In this study, we developed a cost and time saving one-step multiplex RT-PCR for the simultaneous detection and differentiation of swine influenza viruses (SIV) and 2009 pandemic influenza H1N1 virus (pH1N1). The one-step multiplex RT-PCR using four sets of primer was confirmed to be capable of detection of all SIV subtypes and differential diagnosis of major SIV subtype H1, H3 and pH1N1 on individual or mixed viral culture samples. The sensitivity of the multiplex RT-PCR was determined to be at least $2^{-6}$ $HA/25{\mu}L$ of the presented SIVs, providing sufficient efficacy for a routine SIV monitoring in diagnostic laboratories. In addition, compared with the conventional RT-PCR methods that cannot avoid the carryover DNA contamination, the developed RT-PCR applied with the uracil DNA glycosylase (UNG) system was proven to prevent a false positive reaction by carryover contamination of the pre-amplified DNA. In conclusion, the one-step RT-PCR with UNG system could be applicable to detect and differentiate of SIV from the viral cultures without worry of carryover DNA contamination in clinical laboratories.

• 한국정밀공학회지
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• 제28권5호
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• pp.614-622
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• 2011

An internal lobe pump is suitable for oil hydraulics of machine tools, automotive engines, compressors, constructions and other various applications. In particular, the pump is an essential machine element of an automotive engine to feed lubricant oil. The subject of this paper is the theoretical analysis of internal lobe pump whose the main components are the rotors: usually the outer one is characterized by lobe with multiple profile(ellipse1, involute and ellipse2) shapes, while the inner rotor profile is determined as conjugate to the other. Also, the design of outer rotor depends on new applications with removing carryover phenomenon. The system generates new lobe profile and calculates automatically the flow rate and flow rate irregularity according to the lobe profile generated. In order to obtain rotor shapes in performance and to find optimize the design parameters, a Taguchi method is proposed in this paper. Results obtained from the analysis enable the designer and manufacturer of oil pump to be more efficient in this field.

• Communications for Statistical Applications and Methods
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• 제6권2호
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• pp.601-610
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• 1999

Crossover design is officially except for special occasions recommended by KFDA and FDA for assessing Bioequivalence between two drugs one for reference and the other for innovator. Such design is regarded as a special case of latin square split-polt or repeated measurement design and its main difference with other designing methods is that each subject in an experiment is exposed two drugs in sequence. Therefore general statistical analysis is not suitable since the model for this experiment includes carryover effect in addition to period and sequence effect. in this paper analysis for crossover model with two drugs and its interpretation are mainly discussed and an example is given for illustration.

• Communications for Statistical Applications and Methods
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• 제10권1호
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• pp.115-123
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• 2003

In this paper, we discuss the usefulness of AB/BA/AA/BB crossover design sense of sample size. The result is that the AB/BA/AA/BB crossover design is more economical than AB/BA one if large carryover effect exists and so is it in the comparison with completely randomized design within-subject correlation is large.

• Journal of Pharmaceutical Investigation
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• 제34권5호
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• pp.379-383
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• 2004

Statistical interpretations in a bioequivalence trial are considered and studied when the missing observations occurred in $2\;{\times}\;2$ crossover experiment. Patel (1985) suggested the approximate test procedures for carryover effect and drug effect in $2\;{\times}\;2$ crossover design when some of data are missing in the second period. A modified Patel method is newly proposed to the bioequivalence trial and it is compared with the current method through the simulation study.