• Journal of Korean society of Dental Hygiene
• /
• v.1 no.2
• /
• pp.213-225
• /
• 2001

Objectives: This study was taken to investigate factors related to dental caries in deciduous teeth of preschool children. Methods : this study was completed by oral examination on 623 kindergarten pupils and questionnaire on their mothers in Sangju city from April 1 to May 20, 2001. Study design is Cross-sectional Study. Results: The mean values for decayed teeth indexed(dt index), filled teeth index(ft index), and decayed and filled teeth index(dft index) was 2.46, 1.85, and 4.30 respectively. 2.05 for girl was significantly lower than 2.77 of boy in gender (p<0.01). As child ren got older, values were higher(0.7 and 2.84 of three years old, 1.6 and 4.01 of four, 2.42 and 5.02 of five) in ft and dft index(p<0.001). Also, Mother age was almost same, that is, 4.12 dft value for 30~34 years was smaller than 6.17 for above forties(p<0.001). 2.07 and 4.68 for mothers who graduated from high school were higher than 1.36 and 3.34 for university in the ft and dft index(p<0.05), that is, the more they had a high educational level, the lower they got a values. Value 2.76 for children who had non cariogenic food was lower than 5.11 for those who had cariogenic food in dft index(p<0.001). In dft index, 3.82 of children who have learned the education on oral health from mother frequently have lower than 45 of children who have never learned (p<0.05). The ft value of children who had a snack food with mother(1.29) was a smaller than children had it at out of home(1.97)(p<0.05). The variables related 10 dft index were children and mothers age, educational level, and kinds of snack food (p<0.05). Conclusion : This study reveals that younger girl, younger mother, higher educational level, and non cariogenic foods showed lower dft index.

• Journal of the Korea Convergence Society
• /
• v.11 no.7
• /
• pp.273-280
• /
• 2020

Upon investigating the dental health of teeth and gum, and the cleanliness of the oral cavity of female college students in the early 20's, it was reported to be that the number of cariogenic bacteria was heavily related to the acidity of saliva or ammonia. Also, cariogenic bacteria and ammonia was found out to be related with proteins (p<0.01), and ammonia, the synthetic product of cariogenic bacteria was heavily related to blood and white blood cells (p<0.01). To conclude, the healthiness of teeth was related to the acidity of saliva and ammonia, the healthiness of gum was related to blood, proteins and white blood cells while the oral hygiene was most likely to be related to ammonia. This result matched to the seven oral disease cause analyses of the saliva analysis apparatus (Sill-Ha ST-4910), which implied further utilization of this data to clinical implications. However, this study is limited to young personnel who does not have any chronic or general diseases; therefore further study is required to generalize this study.

• Korean Journal of Pharmacognosy
• /
• v.47 no.1
• /
• pp.43-48
• /
• 2016

The aim of the study was performed to examine the anticariogenic potential of purified bee venom (Apis mellifera L., PBV) collected using bee venom collector from cariogenic bacteria, Streptococcus mutans, Streptococcus sanguis, Porphyromonas gingivalis, and Fusobacterium nucleatum. The anticariogenic effect of purified bee venom was evaluated by agar well diffusion method, minimum inhibitory concentraion (MIC), minimum bactericidal concentration (MBC), and postantibiotic effect (PAE). The human lower gingiva epithelial cell cytotoxicity of purified bee venom was also evaluated. Purified bee venom exhibited significant inhibition of bacterial growth of S. mutans, S. sanguis, P. gingivalis, and F. nucleatum with MIC value of 0.68, 0.85, 3.49, and $2.79{\mu}g/ml$, respectively. The MBC value of purified bee venom against S. mutans, S. sanguis, P. gingivalis, and F. nucleatum was 1.34, 1.67, 8.5, and $6.8{\mu}g/ml$. Furthermore, the results of PAE values against S. mutans, S. sanguis, P. gingivalis, and F. nucleatum showed the bacterial effect with 3.3, 3.45, 2.0, and 2.0. The concentration below 1 mg/ml of purified bee venom had no cytotoxicity in the human lower gingiva epithelial cell. These results suggested that purified bee venom have great potenial as anticariogenic agents.

• International Journal of Oral Biology
• /
• v.42 no.1
• /
• pp.25-31
• /
• 2017

Dental caries is the most common chronic disease in the dental field. Streptococcus mutans (S. mutans) is the most important bacteria in the formation of dental plaque and dental caries. In a previous study, we confirmed that the essential oil of Chrysanthemum boreale has antibacterial activity against S. mutans. Alpha-pinene is one of the major chemical components of Chrysanthemum boreale essential oil. In the present study, we investigated the inhibitory effects of ${\alpha}-pinene$ on cariogenic properties such as growth, acid production, biofilm formation, and bactericidal activity on S. mutans. Alpha-pinene at a concentration range of 0.25-0.5 mg/mL significantly inhibited the growth of S. mutans and acid production of S. mutans. Biofilm formation was significantly inhibited at > 0.0625 mg/mL ${\alpha}-pinene$, similar to the data from scanning electronic microscopy. Under confocal laser scanning microscopy, the bacterial viability was decreased by ${\alpha}-pinene$ in a dose-dependent manner. These results suggested that ${\alpha}-pinene$ may be a useful agent for inhibiting the cariogenic properties of S. mutans.

• Journal of dental hygiene science
• /
• v.12 no.2
• /
• pp.79-84
• /
• 2012

The purpose of this study was to utilize it as useful basic data for improving oral health and for developing educational guidelines, by surveying the intake frequency in cariogenic food and the dietary intake status in detergent food through dietary life diary of some female undergraduates, and by researching into relationship between dietary intake factor and dental caries. It allowed dietary life diary for 5 days to be recorded targeting female undergraduates for the Dept. of Dental Hygiene, selected dietary life diary of 66 people without omission of record, and used it in this study. The following conclusions were obtained. 1. As a result of surveying the food intake status, it was indicated to be 12.82 for cariogenic food intake frequency, 256.36 for caries incidence available time and 8.32 for detergent food intake frequency. 2. As a result of surveying in order to examine relationship between cariogenic food intake frequency, detergent food intake frequency & cariogenic light-meal intake frequency and dental caries by dividing group with caries and group with non-caries, the group with caries was indicated to be a little higher than the group with non-caries. However, statistically significant difference wasn't indicated. 3. In the correlation between dietary intake status and dental caries, it was indicated that the more rise in cariogenic food intake frequency leads to the more rise in detergent food intake frequency and even in total light-meal intake frequency, but wasn't shown correlation with dental caries. In light of the above results, many limitations were indicated in finding and analyzing the dental caries incidence level and cause just with a method through surveying dietary intake. However, given the more in cariogenic food intake frequency and detergent food intake frequency, the possibility of dental caries incidence could be predicted.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.29 no.2
• /
• pp.189-194
• /
• 2015

Streptococcus mutans (S. mutans) is one of the most important bacteria in the formation of dental plaque and dental caries. S. mutans adheres to an acquired pellicle formed on the tooth surface, and aggregates with many oral bacteria, and initiates plaque formation by synthesizing glucan from sucrose, which is catalyzed by glucosyltransferases. S. mutans metabolizes the dietary sugar to the organic acids. The organic acids demineralize tooth surface and result in dental caries. Galla Chinensis have been traditionally used for stopping bleeding of gingiva, removing edema and halitosis, drainage, fixing the teeth and as an antiphlogistic agent. In previous reports, antibacterial effects of Galla Chinensis have been investigated whereas anti-cariogenic effects is still not examined enough. Therefore we tested effects of ethanol extracts of Galla Chinensis on the cariogenic properties such as the growth, acid production, adhesion, and insoluble glucan synthesis of S. mutans. In the result, ethanol extracts of Galla Chinensis showed the inhibition of S. mutans growth and organic acids production over 0.031 mg/ml concentrations. The adhesion of S. mutans to Saliva-coated Hydroxyapatite beads S-HAs has decreased with the increase of concentration of ethanol extracts of Galla Chinensis. And it seems to have adhesion inhibitory effect in concentration of over 0.25 mg/ml. It gives us the result that Galla Chinensis have anti-caries effects. But ethanol extract of Galla Chinensis didn't have inhibitory effect on insoluble glucan synthesis. Preliminary phytochemical analysis of the ethanol extract of Galla Chinensis showed strong phenolic compounds, medium steroids & terpenoids and glycosides, and weak organic acids and peptides. These results suggest that the ethanol extracts of Galla Chinensis may have anti-cariogenic properties, which may be able to be related with strong phenolic compounds.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.43 no.4
• /
• pp.391-400
• /
• 2016

The purpose of this study was to evaluate the difference of remineralization effects of various anti-cariogenic toothpastes on artificial carious lesions in primary and permanent teeth using quantitative light-induced fluorescence-digital (QLF-D) system. Sound human primary (n = 48) and permanent teeth (n = 48) were randomly divided into following groups : control group (Group 1), fluoride toothpaste (Group 2), functionalized tricalcium phosphate (fTCP) + fluoride toothpaste (Group 3), and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) toothpaste (Group 4). Specimens were prepared by exposure in a demineralizing solution and then treated using the different toothpastes twice daily during 14 days. All specimens were analyzed with the QLF-D system. QLF data analysis indicated three different toothpastes showed significant remineralizing effects compared to Group 1 in both primary and permanent teeth. Also, the remineralizing effects in Group 3 and 4 were significantly higher than in Group 2. This study suggested that the toothpastes containing fTCP + fluoride and CPP-ACP have the significant anti-cariogenic effects on enamel demineralization in both primary and permanent teeth, and QLF-D is an useful device to assess the incipient carious lesion and remineralization effects of the anti-cariogenic materials quantitatively. Therefore, clinicians can consider the QLF-D system for the evaluation of demineralization and remineralization in primary and permanent teeth.

• The korean journal of orthodontics
• /
• v.35 no.4 s.111
• /
• pp.312-319
• /
• 2005

Streptococcus mutans and Streptococcus sobrinus are major etiological agents in enamel demineralization around orthodontic appliances. This study was designed to examine the prevalence of these streptococci on orthodontic brackets in vivo using polymerase chain reaction. Four incisor brackets in the upper and lower arches were removed and collected from 80 patients at the time of debonding. The genomic DMA of adhered bacteria was extracted and each dextranase gene of S. mutans and S. sobrinus was amplified using the specific oligonucleotide primers. The results showed that the maxillary incisor brackets were colonized by both cariogenic streptococci to a somewhat higher degree than that taken from the mandible. The prevalence of S. mutans was $50.0\%$ on the maxillary incisor brackets and $33.8\%$ on the mandibular incisor brackets, and that of S. sobrinus was $17.5\%$ and $15.0\%$, respectively. Both species were detected on the maxillary incisor brackets of 7 patients $(8.8\%)$ and the mandibular incisor brackets of 5 patients $(6.3\%)$. These results suggest that cariogenic streptococci can adhere to the incisor brackets and may be resident species on the incisor brackets.

• Journal of Korean society of Dental Hygiene
• /
• v.1 no.1
• /
• pp.39-51
• /
• 2001

This study was performed to collect basic data for the development of effective oral health education program of lower graders in elementary school. The subject in this study were 704 first-year, second-year and third-year in Hyoje elementary school. The authors collected the tissue where dental caries involved. the possibilities of dental caries prevention. cariogenic food and noncariogenic food. time and frequency of toothbrushing. size of using toothbrush, intake frequency of cariogenic food and the experience with visiting dental clinics, the purpose of visiting dental clinics and fear for dental treatment. The collected data were analyzed. The finding of this study were as follows: 1. Twenty-four percent of students had not correct understanding about the tissue which dental caries are involved. Eleven percent of students thought that it was impossible to prevent dental caries. 2. Sixty-four percent of student brushed their teeth before going to bed, and 63.0% of responded students brushed their teeth twice or more a day. Seventy-eight percent of students used small toothbrush. and there was a Significant difference of size of using toothbrush among the first, second and third graders(P<0.01). Sixty-nine percent of students intake cariogenic food once or more a day. 3. Eighty-six percent of students had experienced with visiting denial clinics. sixty-two percent of students visited dental clinics when they had a toothache. and there was a significant difference of the purpose of visiting dental clinics among the first. second and third graders(P<0.01). Seventeen percent of students responded that dental treatment is fearful there was a significant difference of fear for denial treatment among the first, second and third graders(P<0.05). 4. It was recommended that effective oral health education programs should be developed to increase toothbrushing after breakfast, to decrease intake frequency of cariogenic food, to increase frequency of visiting dental clinics regularly, and to reduced fear for dental treatment.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.47 no.4
• /
• pp.397-405
• /
• 2020

The aim of this study is to compare cariogenic characteristics of fluoride-sensitive Streptococcus mutans [fluoride-sensitive (FS) S. mutans ] and fluoride-resistant Streptococcus mutans [fluoride-resistant (FR) S. mutans] in the presence of sucrose, and to evaluate its effect on cariogenic biofilm formation. S. mutans ATCC 25175 was continuously cultured in trypticase soy broth (TSB) containing NaF (70 ppm) for 40 days to generate FR S. mutans. FS and FR S. mutans were inoculated in TSB with or without 2% sucrose, and optical density and pH were measured every hour. An oral biofilm was formed using saliva bacteria and analyzed through confocal laser scanning microscopy and CFU count. Finally, the expression of glucosyltransferases genes of both S. mutans was investigated through RT-PCR. FR S. mutans exhibited slower growth and lower acidogenicity in the presence of sucrose compared to FS S. mutans . Both cariogenic and single species biofilm formation was lower in the presence of FR S. mutans, along with reduced number of bacteria. FR S. mutans showed significantly low levels of gtfB, gtfC, and gtfD expression compared to FS S. mutans . On the basis of results, FR S. mutans may be less virulent in the induction of dental caries.