• Journal of Microbiology and Biotechnology
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• v.3 no.2
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• pp.86-90
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• 1993

CMCase positive clones were screened from Cellulomonas sp. YE-5 and named pCE1, pCE2 and pCE3. Among the positive clones pCE1 was used for this study, because it has the smallest insert and the highest CMCase activity among the 3 clones, and its nucleotide sequence was determined. The CMCase gene in pCE1 was composed of 1071 bp of nucleotides coding 357 amino acids. Computer analysis showed that the pCE1 has 65% sequence homology with the endoglucanase from Cellulomonas fimi.

• Journal of the Korean Society of Tobacco Science
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• v.12 no.1
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• pp.9-18
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• 1990

To study the determinants which are involved in the loss of pathogenicity in wilt-inducing Pseudomoms solamcewum, several physlologica I functions were compared in a virulent P. solanacearum strain and an avirulent, spontaneously derived mutant strain. The polyacrylamide gel electrophoresis showed the distinction between two strains in the patterns and the relative intensity of proteins produced intracellularly or extracellularly. Enzyme assays showed that the level of polygalacturonase activity in the culture filtrate of the avirulent mutant was markedly reduced, while carboxymethylcellulase(rondoglucanase) activity in both strains were nearly negligible. These results suggest that the loss of pathogenicity in mutant strain is attributed in part to the reduced production of polygalacturonase. In audit ion, comparative analyses by agarose gel electrophoresis of DNA molecules isolated from both strains show that the pathogenicity genes of p. solanaceerum are not located on plasmid but are on chromosome.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.525.3-526
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• 1986

For the analysis of fermentation characteristics and productivity of plasmid coded product, car-boxymethylcellulase in a recombinant DNA cell fermentation system, batch and continuous fermentations were carried out using a Bacillus megaterium ATCC 14945 transformed with a plasmid, pCK 108 haboring carboxymethyl cellulase gene. The effects of carbon and nitrogen sources and of temperature and pH on cell growth, product yield, plasmid stability, specific plasmid contents of cell, and gene expression efficiency were carefully studied. These experimental results will be discussed in some details.

• Microbiology and Biotechnology Letters
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• v.25 no.2
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• pp.115-120
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• 1997

Penicillium sp.-L4, a causative fungus of rot in citrus fruits, was isolated and its mode of hydrolytic enzyme production was investigated. Carboxymethylcellulase (CMCase), polygalacturonase(PGase), extra- & intra-cellular $\beta$-glucosidase and cellobiase were produced drastically by addition of substrates in minimal media. Production of the hydrolytic enzymes were induced efficiently by cellobiose and cellooligosaccharides which were the products of cellulose hydrolysis, but repressed by addition of mono-saccharide such as glucose, raffinose, galacturonic acid. The relative activity of p-nitrophenyl-$\beta$-D-glucopyranoside(PNPG) hydrolysis was higher than that of cellobiose hydrolysis in extracellular enzymes, and reverse is true in intracellular enzymes. Intact enzyme production of P. sp.-L4 on lemon peel lesion was sequential. $\beta$-Glucosidase and CMCase were produced first and followed by PGase. The enzyme productivities and pH in lesions were coincident with optimal pH of each enzyme activities.

• Korean Journal of Microbiology
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• v.18 no.3
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• pp.133-147
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• 1980

Washed mycelia of Aspergillus nidulans FGSC159 were incubated in CMC minimal liquid medium and the culture filtrate which contained induced extracellular cellulase was fractionated by a three-step procedure including chromatography on Bio-Gel P-150, chromatography on DEAE-Sephadex A-50 and chromatography on Sephadex G-100. Three CMCase components ; F-I-Ia, F-I-Ib and F-II-Ia were prepared. No enzyme activity toward avicel could be detected in these components. Similarly, there was no ${\beta}-glucosidase$ activity. pH-optima of the three components were all 5.0 in acetate buffer. Temperature-optima for the activities of F-I-Ia, F-Ib and F-II-Ia were $45^{\circ}C,\;40^{\circ}C\;and\;50^{\circ}C$, respectively. F-II-Ia was shown to be more thermostable than the other two components. F-II-Ia was proved to have quite a different substrate specificity and action property and action property from those of F-I-Ia and F-I-Ib by product analysis on liquid chromatography.

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.113.1-113.1
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• 2007

See Full Text

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.114.1-114.1
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• 2007

See Full Text

• Journal of Life Science
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• v.23 no.6
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• pp.757-769
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• 2013

A microorganism producing carboxymethylcellulase (CMCase) was isolated from seawater, identified as Bacillus velezensis by analyses of 16S rDNA and partial sequences of the gyrA, and designated as B. velezensis A-68. The optimal conditions for production of CMCase by B. velezensis A-68 were established using response surface methodology (RSM). The optimal concentrations of rice hulls and yeast extract, and initial pH of the medium for cell growth were 60.2 g/l, 7.38 g/l, and 7.18, respectively, whereas those for production of CMCase were 50.0 g/l, 5.00 g/l, and 7.30. The analysis of variance (ANOVA) implied that the most significant factor for cell growth as well as production of CMCase was yeast extract. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}7H_2O$, and $(NH_4)_2SO_4$ in the medium for cell growth were 7.50, 1.00, 0.10, and 0.80 g/l, respectively, which were the same as those for production of CMCase. The optimal temperatures for cell growth and production of CMCase were 30 and $35^{\circ}C$, respectively. The maximal production of CMCase under optimized conditions was 83.8 U/ml, which was 3.3 times higher than that before optimization. In this study, rice hulls, agro-byproduct, were developed as a substrate for production of CMCase and time for production of CMCase was reduced to 3 days using a newly isolated marine bacterium.

• Research in Plant Disease
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• v.18 no.3
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• pp.240-244
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• 2012

Burkholderia gladioli pv. alliicola CH1 showed typical soft rot symptoms at higher than $20^{\circ}C$ but very weak soft rot symptoms at temperature under $10^{\circ}C$. Among the nine agro-chemicals, oxolinic acid WP, streptomycin + copper hydroxide WP and streptomycin WP were found to be effective for the inhibition of the pathogen in vitro. The results of scanning electron microscopic investigation showed that onion bulbs was macerated by infection of B. gladioli pv. alliicola CH1. B. gladioli pv. alliicola CH1 was able to produce polygalacturonase but did not produce pectin lyase and carboxymethylcellulase. In analysis of the polygalacturonase activity of the isolated pectin-degradation enzymes from B. gladioli pv. alliicola CH1 total protein, three activity bands 45 kDa, 35 kDa, and 29 kDa were detected by the direct (or in-gel) activity staining on SDS-PAGE.

• The Korean Journal of Mycology
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• v.42 no.1
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• pp.40-49
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• 2014

The mycelial growths of wood-decaying mushroom strains collected from Korean forests were investigated on solid media under different culture media and temperatures. Most of strains showed the higher mycelial growth on potato dextrose agar (PDA) than malt extract agar (MEA) or sabouraud dextrose agar (SDA) plates. Except for a few strains, they grew well on PDA at $25^{\circ}C$ and showed a poor growth at low temperature ($10^{\circ}C$) than high temperature ($30^{\circ}C$). All strains showed the carboxymethylcellulase (CM-cellulase) and laccase activities on solid media containing the specific substrates for two different enzymes.