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http://dx.doi.org/10.5423/RPD.2012.18.3.240

Phytopathogenicity of Burkholderia gladioli pv. alliicola CH1 and Production of PGase Isozymes  

Lee, Chan-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Jong-Tae (Onion Research Institute, Gyeongnam Agricultural Research and Extension Services)
Kim, Yeong-Tae (Genomics Division, National Academy of Agricultural Science, Rural Development Administration)
Jhune, Chang-Sung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Cheong, Jong-Chun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Park, Wan (School of Life Sciences and Biotechnology, Kyungpook National University)
Publication Information
Research in Plant Disease / v.18, no.3, 2012 , pp. 240-244 More about this Journal
Abstract
Burkholderia gladioli pv. alliicola CH1 showed typical soft rot symptoms at higher than $20^{\circ}C$ but very weak soft rot symptoms at temperature under $10^{\circ}C$. Among the nine agro-chemicals, oxolinic acid WP, streptomycin + copper hydroxide WP and streptomycin WP were found to be effective for the inhibition of the pathogen in vitro. The results of scanning electron microscopic investigation showed that onion bulbs was macerated by infection of B. gladioli pv. alliicola CH1. B. gladioli pv. alliicola CH1 was able to produce polygalacturonase but did not produce pectin lyase and carboxymethylcellulase. In analysis of the polygalacturonase activity of the isolated pectin-degradation enzymes from B. gladioli pv. alliicola CH1 total protein, three activity bands 45 kDa, 35 kDa, and 29 kDa were detected by the direct (or in-gel) activity staining on SDS-PAGE.
Keywords
Burkholderia gladioli pv. alliicola; Polygaractronase; SDS-PAGE;
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