• Journal of Pharmaceutical Investigation
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• 제36권4호
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• pp.253-262
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• 2006

This study was performed to investigate the effects of treatment for dermatitis using the herbal gel preparations. Scutellariae Radix(SR) and Zingiberis Rhizoma(ZR) were used for the purpose of herbal preparations. Baicalin, baicalein are the ingredients of SR, having biological effects like anti-inflammatory, anti-oxidative, anti-bacterial and antiallergic action. 6-Gingerol is one of the ingredients of ZR having biologicai effects like anti-inflammatory and analgesic action. The three types of hydrogels(SRE, SRH, SZH) were formulated with Carbopol 940, Labrasol, Triethanolamine etc. Baicalin was hydrolysed to baicalein by $\beta$-glucuronidase for the purpose to increase rate of skin permeation. Content of ingredients by HPLC determination, rate of skin permeation using Franz type diffusion cell, anti-oxidative activity for the free radical, hydroxyl radical, superoxide, anti-inflammatory by using carrageenan injection, efficacy on the dermatitis induced by 2,4-dinitro-chlorobenzene(DNCB) were experimented. Baicalein showed higher permeability than baicalin, so it is considered that baicalein was more suitable form than baicalin for transdermal absorption by its lipophilic property. In the anti-oxidative experiments, SZH gel was the most effective scavenging activity than the other gels in all experiments. In anti-inflammatory test, SRM and SZH gel more decreased edma rapidly than SRE gel. In case of using SZH gel, treatment period for the dermatitis was more declined than that of other gel groups. These results suggests that the SZH hydrogel could be suitable preparations for the treatment of dermatitis.

• Journal of Pharmaceutical Investigation
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• 제30권3호
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• pp.179-189
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• 2000

Clebopride malate(Cm) is a new benzamide drug which has a potent central antidopaminergic activity possessing antiemetic and anxiolytic properties. A purpose of this study was to assess the feasibility of formulating sustained release preparation by dispersing a drug in hydrophilic polymeric matrices and double layered tablets(DLT), using HPMC, carbopol, PEO, PVP/VA and other polymers as a rate controlling barrier. The matrix and DLT showed optimum dissolution pattern up to 8 hours and the contents of polymer were optimized at 30% level in this preparation. After an oral administration in beagle dog, Cm concentration was determined by use of GC-ECD and pharmacokinetic parameters were calculated by Vallner's method. The AUC of DLT showed similar results and the duration of action was increased 55% compared to that of regular release dosage form. The calculated absorption rate effectiveness(ARE) and controlled release effectiveness(CRE) for DLT increased 50% compared to that of matrix, the overall effectiveness(E) of this product appears to be about 70%. in vivo effectiveness test, DLT showed significant differences from control on antiemetic action of Cm. In consequence, it was possible to conclude that double layered tablet might be a good candidate for the sustained release dosage forms.

• Journal of Ginseng Research
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• 제42권4호
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• pp.512-523
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• 2018

Background: 20(S)-Protopanaxadiol (20S-PPD) is a fully deglycosylated ginsenoside metabolite and has potent dermal antiaging activity. However, because of its low aqueous solubility and large molecular size, a suitable formulation strategy is required to improve its solubility and skin permeability, thereby enhancing its skin deposition. Thus, we optimized microemulsion (ME)-based hydrogel (MEH) formulations for the topical delivery of 20S-PPD. Methods: MEs and MEHs were formulated and evaluated for their particle size distribution, morphology, drug loading capacity, and stability. Then, the deposition profiles of the selected 20S-PPD-loaded MEH formulation were studied using a hairless mouse skin model and Strat-M membrane as an artificial skin model. Results: A Carbopol-based MEH system of 20S-PPD was successfully prepared with a mean droplet size of 110 nm and narrow size distribution. The formulation was stable for 56 d, and its viscosity was high enough for its topical application. It significantly enhanced the in vitro and in vivo skin deposition of 20S-PPD with no influence on its systemic absorption in hairless mice. Notably, it was found that the Strat-M membrane provided skin deposition data well correlated to those obtained from the in vitro and in vivo mouse skin studies on 20S-PPD (correlation coefficient $r^2=0.929-0.947$). Conclusion: The MEH formulation developed in this study could serve as an effective topical delivery system for poorly soluble ginsenosides and their deglycosylated metabolites, including 20S-PPD.

• 보존과학회지
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• 제37권4호
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• pp.370-379
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• 2021

In the past, PVAc [poly(vinyl acetate)] was used as a fixative for the conservation of the murals in the murals of the Payathonzu temple located in Bagan, Myanmar. In this study, attention was paid to gel cleaning as a method for stably removing such PVAc fixatives. Based on the recent research trend related to the cleaning of murals in Bagan ruins, 3 types of gelling agents (Nevek^®, Laponite^®RD, Carbopol^®980) and 2 types of organic solvents (Acetone, Dimethyl carbonate) were selected. Six types of gel cleaners were prepared by mixing gelling agents and organic solvents, and the properties and fixative removability of these cleaners were compared. As a result of confirming the properties of the prepared gel cleaners, the pH of the cleaners was all in the weak acidic to weakly alkaline range, which was a stable condition for mural application. Also, there was no difference in the viscosity of the cleaners depending on the type of solvent, but there was a difference depending on the type of gelling agent used. Regarding the weight loss ratio of PVAc, which is an indicator of removability, the exposure conditions of the gel cleaners, the boiling point of the solvent used, and the viscosity of the gelling agent acted as factors affecting. As a result of comparing the removability of gel cleaners, it was confirmed that the solvent's fixative solubility, the volatility of the solvent itself, and the solvent release control properties of the gelling agent had a great effect on the removability of the gel cleaners. In Part 2, the stability and the running applicability of the gel cleaners will be investigated by making mockup samples reflecting the properties of the materials and techniques used to produce the mural paintings in the Payathonzu Temple.

• 대한소아치과학회지
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• 제36권2호
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• pp.199-208
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• 2009

본 연구는 기존의 $DIFOTI^{TM}$ 시스템의 문제점을 개선하고자 LED와 디지털 카메라를 결합한 발전된 형태의 prototype DIFOTI시스템을 만들고, 이를 기존 시스템과 비교 평가하기 위하여 인공 우식 용액으로 탈회시킨 법랑질 시편에 대하여 1일부터 7일까지 영상을 촬영하고 분석하여 다음과 같은 결과를 얻었다. 1. 실험 기간에 따라 두 시스템 모두 우식 법랑질의 광투과도(index of transillumination)가 감소하는 양상을 보여 주었다. 2. $DIFOTI^{TM}$ 시스템에 비해 prototype DIFOTI 시스템에서 건전 법랑질과 탈회 법랑질 간의 발광도(intensity of luminance) 차이가 더 적게 나타나는 양상을 보였다. 이상의 결과를 종합해보면 prototype DIFOTI 시스템은 전류 소모량이 적은 LED를 사용하여 배터리를 사용한 무선 시스템으로의 개발 가능성을 보여 주었고, 디지털 카메라는 디지털 영상 기술의 발달과 함께 무선 전송 시에도 매우 높은 화질을 기대할 수 있어 차후에 보다 작고 사용이 편리한 DIFOTI 시스템을 개발할 수 있는 가능성이 크다 할 수 있다.

• 대한치과교정학회지
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• 제40권4호
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• pp.212-226
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• 2010

치간 삭제 후 재광화 및 치아우식 예방법을 찾기 위한 노력의 일환으로 1.23% 인산화불소 젤 또는 5%, 10% 수산화인회석함유 페이스트를 치간 삭제된 법랑질 표면에 도포하였고, 그 후 젖산탈회용액으로 처리하였다. 법랑질 표면의 재광화 및 탈회 후 결정구조 변화를 주사전자현미경으로 관찰하고 칼슘, 인, 불소 함량 등의 변화를 energy dispersive X-ray spectroscopy로 분석해서 비교하였다. 치간 삭제 후 5%, 10% 수산화인회석함유 페이스트를 도포한 군에서 칼슘과 인 함량이 증가하였고, 1.23% 인산화불소 젤 도포 군에서는 불소 함량이 증가하였다. 또한 모든 재광화 제제에서 법랑질 표면이 매끄럽고 부드러워졌으며 결정구조가 작고 치밀해짐을 관찰할 수 있었다. 10% 수산화인회석함유 페이스트로 재광화한 군은 탈회 후에도 칼슘과 인 함량이 증가된 채로 유지되었고, 5% 수산화인회석함유 페이스트로 재광화한 군은 탈회 후 인 함량이 증가된 채로 유지되었다. 치간 삭제 후 탈회 처리 시 대조군의 법랑질은 파괴양상이 심해 거친 굴곡으로 이루어진 불규칙한 표면 상태를 나타내었으나 불소 및 수산화인회석으로 재광화한 군에서는 법랑질의 파괴양상이 적어 작은 결정입자들과 미세공극이 유지되었다.

• 한국치위생학회지
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• 제12권2호
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• pp.335-343
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• 2012

Objectives : The aim of this investigation was to evaluate the remineralization effect of Bamboo salt and NaF+Bamboo salt solutions on bovine enamel formed incipient artificial enamel caries by microcomputed tomography (micro CT). Methods : Experimental solutions were distilled water(negative control), 2% sodium fluoride solution (2% NaF group), 3% bamboo salt solution (3% BS group) and the solution mixed 2% sodium fluoride solution and 3% bamboo salt solution (2% NaF+3% BS group). Specimens were prepared from extracted bovine teeth and divided into 4 groups of 10 specimens each by randomized blocks according to density. Then the specimens surface were divided equally into three parts to observe sound enamel area, incipient enamel carious area and remineralized enamel area. Only one-third of specimen surface was coated with nail varnish and these were exposed to a lactate carbopol buffer system for 72 hours. Then one-half of the demineralized enamel area was coated with nail varnish. The specimens were carried out under pH cycling model for 14 days as follows; samples were immersed in each experimental solution for 2 mins 3 times per day, demineralized for 4 hours and in mixed saliva for the remaining hours. After pH cycling, density was measured using micro CT. Results : All experimental groups showed remineralization effects except for negative control group(p<0.05). The differences of density after experimental solution treatment were statistically significant difference among 4 groups (p<0.01). The density difference values of groups were $0.04{\pm}0.01$ in negative control group, $0.19{\pm}0.01$ in 2% NaF group, $0.14{\pm}0.01$ in 3% BS group, and $0.21{\pm}0.01$ in 2% NaF+3% BS group. Conclusions : The bamboo salt solution showed remineralization effects on incipient artificial enamel caries and the solution mixed sodium fluoride solution and bamboo salt solution showed more remineralization effects than the bamboo salt solution. Thus, it is suggested that bamboo salt can be used as remineralization agent in incipient enamel caries lesion.

• The Korean Journal of Physiology and Pharmacology
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• 제19권6호
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• pp.543-547
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• 2015

We investigated the combined moisturizing effect of liposomal serine and a cosmeceutical base selected in this study. Serine is a major amino acid consisting of natural moisturizing factors and keratin, and the hydroxyl group of serine can actively interact with water molecules. Therefore, we hypothesized that serine efficiently delivered to the stratum corneum (SC) of the skin would enhance the moisturizing capability of the skin. We prepared four different cosmeceutical bases (hydrogel, oil-in-water (O/W) essence, O/W cream, and water-in-oil (W/O) cream); their moisturizing abilities were then assessed using a $Corneometer^{(R)}$. The hydrogel was selected as the optimum base for skin moisturization based on the area under the moisture content change-time curves (AUMCC) values used as a parameter for the water hold capacity of the skin. Liposomal serine prepared by a reverse-phase evaporation method was then incorporated in the hydrogel. The liposomal serine-incorporated hydrogel (serine level=1%) showed an approximately 1.62~1.77 times greater moisturizing effect on the skin than those of hydrogel, hydrogel with serine (1%), and hydrogel with blank liposome. However, the AUMCC values were not dependent on the level of serine in liposomal serine-loaded hydrogels. Together, the delivery of serine to the SC of the skin is a promising strategy for moisturizing the skin. This study is expected to be an important step in developing highly effective moisturizing cosmeceutical products.

• 대한소아치과학회지
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• 제31권3호
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• pp.506-515
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• 2004

치아 우식증을 조기에 발견하여 기존의 외과적 와동 형성을 피하고 건전한 치질을 최대한 보존하고자하는 예방적 시술 개념에 대한 관심과 연구가 최근 유럽을 중심으로 확산되고 있다. 치아 우식증의 임상적 조기 진단을 위해 새로운 장비들이 개발되고 있으며, 이미 실험실 연구를 통해 성능의 우수성이 입증된 바 있다. 본 연구의 목적은 치아 우식증의 조기 진단을 목적으로 새로 개발된 quantitative light induced fluorescence analysis (QLF) 시스템을 이용하여 우치, 사람의 유치 및 영구치의 법랑질 탈회 속도를 비교 평가하고자 하였다. 각 군당 25개씩 배정된 실험 대상 치아들은 표면을 약 $50{\sim}100{\mu}m$정도 제거한 후 활택하여 탈회 용액(0.1mol/L 젖산, 0.2% 카보폴 907 그리고 50%로 농축된 수산화 인회석으로 제조)에서 1, 2, 4 그리고 8일 동안 탈회시켰다. 모든 시편들은 QLF분석을 시행하였으며 실험 일정에 따라 5개의 시편들을 선택하여 gold standard로 사용하기 위해 Transverse Microradiography analysis (TMR)를 시행하였다. TMR 분석을 통하여 무기질의 탈회량(IML)과 병소 깊이(LD)를 측정하였다. QLF 분석에서 측정된 정상 법랑질의 fluorescence radiance (FRs) 값에 대한 탈회 법랑질의 fluorescence radiance $(FR_D)$의 비율인 FR ratio $(FR_D/FR_s)$를 산출하였다. 우치에서는 FR Ratio와 병소 깊이 간에 통계적 유의성이 있었으며(p<0.05), 유치에서는 FR Ratio와 광물질 소실 및 병소 깊이 간에는 유의성이 없었다(p>0.05). 영구치에서는 FR Ratio와 광물질 소실 및 병소 깊이 간에 통계적 유의성이 있었다(p<0.05). 탈회 시간에 대한 FR Ratio의 회귀 분석 결과에서 얻은 회귀 계수 값은 우치에서는 -4.643(p<0.05), 유치에서는 -5.421(p<0,05), 영구치에서 -4.435(p<0.05)로 나타났다.

• Journal of Pharmaceutical Investigation
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• 제40권5호
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• pp.305-311
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• 2010

The present study was aimed at preparing microemulsion-based hydrogel (MBH) for the skin delivery of itraconazole. Microemulsion prepared with Transcutol as a surfactant, benzyl alcohol as an oil and the mixture of ethanol and phasphatidyl choline (3:2) as a cosurfactant were characterized by solubility, phase diagram, particle size. MBHs were prepared using 0.7 % of xanthan gum (F1-1) or carbopol 940 (F1-2) as gelling agents and characterized by viscosity studies. The in vitro permeation data obtained by using the Franz diffusion cells and hairless mouse skin showed that the optimized microemulsion (F1) consisting of itraconazole (1% w/w), benzyl alcohol (10% w/w), Transcutol (10% w/w) and the mixture of ethanol and phospahtidylcholine (3:2) (10% w/w) and water (49% w/w) showed significant difference in the flux (${\sim}1{\mu}g/cm^2/h$) with their corresponding MBHs (0.25-0.64 ${\mu}g/cm^2/h$). However, the in vitro skin drug content showed no significant difference between F1 and F1-1, while F1-2 showed significantly low skin drug content. The effect of the amount of drug loading (0.02, 1 and 1.5% w/w) on the optimized MBH (F1-2) showed that the permeation and skin drug content increased with higher drug loading (1.5%). The in vivo study of the optimized MBH (F1-2 with1.5% w/w drug loading) showed that this formulation could be used as a potential topical formulation for itraconazole.