• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.1
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• pp.15-21
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• 2006

This study was conducted to understand the regulation mechanism of carbohydrate allocation and partitioning of one-year-old seedlings of Betula schmidtii with Cd treatment, and to assess tolerance against Cd toxicity among five half-sib families on the basis of carbon allocation and partitioning. Seedlings were treated with $CdSO_4$ solution of 0, 0.2, 0.4, and 0.6 mM for two months. After harvesting, carbohydrate concentrations were analyzed for leaves, stems and roots of seedlings. Carbohydrate concentration for Cd-treated seedlings decreased in comparison with control plants, even though Cd effects were significantly different among five families. Meanwhile, Cd treatment decreased carbohydrate allocation in leaves and increased allocation in roots. In addition, partitioning of glucose in leaves was increased by Cd treatment, but partitioning of sucrose and starch in leaves decreased. In Cd-treated roots, partitioning of glucose, sucrose and starch increased. On the basis of carbohydrate allocation patterns, 'Family No. 7' (of the five families studied) was considered as the most sensitive family to Cd toxicity because the decrease of carbohydrate concentration and the change of carbohydrate allocation pattern after Cd treatment were relatively greater.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.9-21
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• 2005

To study the biochemical and physiological role of the plastidic glucose transporter (pGlcT) in carbohydrate metabolism, we characterized transgenic plants with mutations in the pGlcT gene (GT), gt-1 and gt-2, as well double mutants of GT and the maltose transporter (MEX1) and GT and the triose phosphate/phosphate translocator (TPT), GT and the cytosolic fructose-1,6-bisphosphatase gene (cFBP), and MEX1 and TPT, gt-1/mex2, gt-1/tpt-2, gt-1/cfbp-1, mex1-1/tpt-2, respectively. Compared to the wild type, all mutants except the gt-1/cfbp-1 mutant lines displayed higher starch accumulation and higher levels of maltose. Starch accumulation is due to a decrease in starch turnover, leading to an imbalance between the rates of synthesis and degradation. Sucrose levels of gt alleles were higher than those in wild-type plants during the light period, suggesting possible nightly supplementation via the maltose transport pathway to maintain proper carbohydrate partitioning in the plant leaves. The gt plants displayed less growth retardation than mex1-1 mutant and gt-1/mex2 double mutant displayed accumulativesevere growth retardation as compared to individual gt-1 and mex1-1 mutants, implying that the maltose transporter-mediated pathway is a major route for carbohydrate partitioning at night. The gt-1/tpt-2, mex1-1/tpt-2 and gt-1/cfbp-1 double mutants had retarded growth and low chlorophyll content to differing degrees, indicating that photosynthetic capacity had diminished. Interestingly, the gt-1/tpt-2 line displayed a glucose-insensitive phenotype and higher germination rates than wild type, suggesting its involvement not only in carbon partitioning, but also in the sugar signaling network of the pGlcT and TPT.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.563-570
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• 2014

The shortage or surplus of minerals directly affects overall physiological metabolism of plants; especially, it strongly influences carbohydrate metabolism as a primary response. We have studied mineral uptake, synthesis and partitioning of soluble carbohydrates, and the relationship between them in N, P or K-excessive tomato plants, and examined the interaction between soluble carbohydrates and mineral elements. Four-weeks-old tomato plants were grown in a hydroponic growth container adjusted with excessive N ($20.0mmol\;L^{-1}$ $Ca(NO_3)2{\cdot}4H_2O$ and $20.0mmol\;L^{-1}$ $KNO_3$), P ($2.0mmol\;L^{-1}$ $KH_2PO_4$), and K ($20.0mmol\;L^{-1}$ $KNO_3$), respectively, for 30 days. Shoot growth rates were significantly influenced by excessive N or K, but not by excessive P. The concentrations of water soluble N (nitrate and ammonium), P and K were clearly different with each tissue of tomato plants as well as the mineral conditions. The NPK accumulation in all treatments was as follows; fully expanded leaves (48%) > stem (19%) = roots (16%) = petioles (15%) > emerging leaves (1). K-excessive condition extremely contributed to a remarkable increase in the ratio, which ranged from 2.79 to 10.34, and particularly potassium was dominantly accumulated in petioles, stem and roots. Fresh weight-based soluble sugar concentration was the greatest in NPK-sufficient condition ($154.8mg\;g^{-1}$) and followed by K-excessive (141.6), N-excessive (129.2) and P-excessive (127.7); whereas starch was the highest in K-excessive ($167.0mg\;g^{-1}$) and followed by P-excessive (146.1), NPK-sufficient (138.2) and N-excessive (109.7). Soluble sugar showed positive correlation with dry weight-based total N content (p<0.01) whereas was negatively correlated with soluble P (p<0.01) and dry weight-based total P (p<0.01). On the other hand, starch production was negatively influenced by total N (p<0.001), but, it showed positive relation with total K concentration (p<0.05). This study shows that uptake pattern of NPK and production and partitioning of soluble carbohydrate were substantially different from each mineral, and the relationship between water soluble- and dry weight-based-mineral was positive.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.1
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• pp.26-32
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• 1995

Weak persistence of white clover （Trifolium repens L.） under continuous grazing has been limited its availability in the mixture with grasses. The experiment was done to determine the effect of defoliation interval on $N_2$ fixation. nitrogen and carbohydrate partitioning of contrasting white clover cultivars. Individual plants of Osceola（large leaf）, Grasslands Huia （me-dium leaf）, and Aberystwyth S184 （small leaf） were grown in 15cm plastic pot containing a 1：2：1 soil：sand：Promix mixture for 55 days, and then clipped to remove all fully expanded leaves every 7, 14 or 28 days. To measure the cultivar response, plants were sampled immediately before final harvest （0） and on 1, 3, 7, 14, and 28 days after the final harvest, and then seperated leaves and petioles, stolons and roots for chemical analysis. Total nonstructural carbohydrate （TNC） concentrations of stolons and roots, and nitrogen con-centration of all the fractions inclined with increased defoliation interval. Those of Osceola, large leaved, were greater than the other cultivars showing different partitioning patterns between stolons and roots. Concentration of TNC was less in roots than in the other fractions while that of nitrogen declined in the order of leaves and petioles, roots and stolons. $N_2$ fixation rate of larger leaved cultivar, Osceola, was higher than that of smaller leaved cultivar. TNC and nitrogen concentrations of all the fractions and $N_2$ fixation rate were reduced as defoliation was imposed, defoliation interval declined or regrowing period become shorter. The partitioning patterns of TNC and nitrogen among the fractions were modified by defoliation interval and cultivars.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.11a
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• pp.137-138
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• 2000

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.273-278
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• 1998

Carbohydrate metabolism and partitioning are dependent on relationships between sources and sinks which can be affected by rates of photosynthesis and respiration. Fructan, the major form of stored carbohydrate in tall fescue (festuca arundineacea Schreb.), changes in concentration during growth and in response to the environment. Objectives of this study were i) to examine the content and the composition of carbohydrates in five tissues (mature leaf blade, immature leaf blade, leaf elongation zone, terminal meristem, and root tips) of two tall fescue genotypes, one with high yield per tiller (HYT) and one with low yield per tiller (LYT), and ii) to compare the reserved and utilized carbohydrates among above five different tissues, particularly between the leaf elongation zone and root tips. The established vegetative tillers of the HYT and LYT genotypes were grown in a controlled-environment growth chamber. Water-soluble carbohydrate (WSC) in the leaf elongation zone was about 22% of dry weight in the HYT and about 19% in the LYT genotype. The root tip also had high WSC, about 12% of dry weight in the HYT and 6% in the LYT genotype. Hexoses and sucrose were the major components of total WSC in all tissues except the leaf elongation zone. The growing tissues (sinks), i.e., the leaf elongation zone and root tip, had a high proportion of low degree of polymerization fructan, i.e., 3 to 8 hexose units.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.157-163
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• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survial or regrowth yield. Dry matter, nitrogen and non-structural carbohydrate content of plants grown under $5^{\circ}C$ or $20^{\circ}C$ of culture temperature during 25 days were investigated. The dry matter content of leaves and roots were significantly reduced under $5^{\circ}C$ compared with $20^{\circ}C$culture condition. Comparing with the dry matter per plant under $20^{\circ}C$, those in leaves and roots under $5^{\circ}C$ decreased to 25% and 10%, respectively, after 25 days of temperature treatment. Total nitrogen content in leaves under $20^{\circ}C$ and $5^{\circ}C$ increased to 68% and 39% compared to the initial lenel(day O), respectively, during 25 days after temperature treatment, Nitrogen content in roots highly increased under 5 C while there was a little change under $20^{\circ}C$ condition. The nitrogen contents in roots under $5^{\circ}C$ and $20^{\circ}C$ were 39.0 and 30.8mgJg DM, respectively, after 25 days of temperature treatment. Total contents of soluble carbohydrate in both leaves and roots under $5^{\circ}C$ were higher than those under $20^{\circ}C$ condition. After 25 days of temperature treatment under$5^{\circ}C$ , their contents in leaves and roots were 1.4 and 2.0 times higher than those of under $20^{\circ}C$ condition. Stach atent in roots under $20^{\circ}C$ was less changed, while thatof under $5^{\circ}C$ greatly increased from 64.8 to 178.7mglg DM duling 25 days. 'Ihese results clearly showed that an accumulation of both nitrogen and non-structural carbohydrate in the plants occured under low temperature condition.e condition.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.9
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• pp.1431-1441
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• 2018

Objective: This study was conducted to assess the effects of replacing rice straw with different proportions of cassava pulp on growth performance, feed intake, digestibility, rumen microbial population, energy partitioning and efficiency of metabolizable energy utilization in beef cattle. Methods: Eighteen yearling Thai native beef cattle (Bos indicus) with an average initial body weight (BW) of $98.3{\pm}12.8kg$ were allocated to one of three dietary treatments and fed ad libitum for 149 days in a randomized complete block design. Three dietary treatments using different proportions of cassava pulp (100, 300, and 500 g/kg dry matter basis) instead of rice straw as a base in a fermented total mixed ration were applied. Animals were placed in a metabolic pen equipped with a ventilated head box respiration system to determine total digestibility and energy balance. Results: The average daily weight gain, digestible intake and apparent digestibility of dry matter, organic matter and non-fiber carbohydrate, total protozoa, energy intake, energy retention and energy efficiency increased linearly (p<0.05) with an increasing proportion of cassava pulp in the diet, whereas the three main types of fibrolytic bacteria and energy excretion in the urine (p<0.05) decreased. The metabolizable energy requirement for the maintenance of yearling Thai native cattle, determined by a linear regression analysis, was $399kJ/kg\;BW^{0.75}$, with an efficiency of metabolizable energy utilization for growth of 0.86. Conclusion: Our results demonstrated that increasing the proportion of cassava pulp up to 500 g/kg of dry matter as a base in a fermented total mixed ration is an effective strategy for improving productivity in zebu cattle.

• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.291-298
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• 2014

Plastic film houses are directly associated with increases in plant growth and yield of vegetable crops through a year round cultivation, however, at the same time temperature stresses are one of fates which are difficult to avoid during crop growth. The objective of this study was to examine the translocation and distribution of minerals (N, P, K) and carbohydrates as well as seasonal fluctuation of mineral uptake and carbohydrate production in cucumber plant grown under moderately high temperature. The temperature treatments consisted of 2-layers film houses (optimal temp.) and 3-layers (high temp.). Shoot growth of cucumber plants were linearly increased until 14 weeks after transplanting (WAT) without any significant difference between both temperatures, and the slowdown was observed from 16 WAT. The level of soluble sugar and starch was slightly greater in optimal temperature compared to the high. Cumulative accumulation of soluble sugar was significantly different before and after 12 WAT in both treatments, whereas starch level represented a constant increase. Monthly production of soluble sugar reached the peak between 12 to 16 WAT, and starch peaked between 4 to 8 WAT and 12 to 16 WAT. Total uptake of N, P and K in optimal and high temperature conditions was $18.4g\;plant^{-1}$ and 17.6 for N, 4.7 and 5.1 for P, and 37.7 and 36.2 for K, respectively, and the pattern of monthly N uptake between optimal and high temperatures was greater in early growth stage, whereas was greater in mid growth stage in both P and K. Thus, this study suggests that moderately high temperature influences much greater to photosynthesis and carbohydrate production than plant biomass and mineral uptake. On the basis of the present result, it is required to indentify analysis of respiration rates from plant and soil by constantly increasing temperature conditions and field studies where elevated temperatures are monitored and manipulated.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.4
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• pp.238-244
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• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survial or regrowth yield. Forage rape(Brassica napus L.) was sown on Sep. 1, 1994. Experimental plots were divided into three replicates under 6 different cutting dates(l0 days interval from Oct. 15 to Dec. 4). Field-grown palnts were sampled on the each cutting date and on the wintering period (Jan. 16) to analyze the nitrogen and non-structural cahohydrate reserves. The rate of winter survival and regrowth yield were also measured in the spring of next year. On the before wintering, dry matter yields were 152, 274, 500, 718, 776 and 981 kg/lOa, respectively, from the cutting date on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. Cmde protein yield significantly increased as cutting date was later until Nov. 14, thereafter a significant increase did not occured. Nitrogen and starch contents per plant significnatly increased as the cutting date was later. The increasing rate of starch was greatly higher than that of nitrogen. On the wintering period, nitrogen reserves in mts were 85.3, 68.8, 47.6, 28.3, 44.3, and 55.3 mglplant, and starch reserve were 11 1.3, 75.3, 39.3, 19.6, 26.4 and 34.6 mglplant, respectively, in the plots cut on Oct. 15, Oct. 25, Nov. 4, Nov. 14, Nov. 24, and Dec. 4. It showed that carbohydrate reserves were much highly utilized than nitrogen reserves during wintering period. The rates of winter survival were 91, 83, 46, 22, 35 and 43% and regrowth yields were 692, 545, 316, 84, 127 and 140 kgD.M/lOa, respectively, in each plots. The highly significant correlation (p<0.01) between the level of organic reserves and the rate of winter survival or regrowth yield were obtained.