• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.150.2-150
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• 2003

Carnation mottle carmovirus(CarMV) was isolated from Lilium spp. in Korea. This isolate, CarMV, was done bioassay, which plants were Dianthus caryophyllus, Gomphrena globosa, Chenopodium amaranticolor, Dianths chinensis. CarMV was propagated on the leaves of Chenopodium amaranticolor with the crude-sap inoculation method and purified by Mossops method(1976). We produced antiserum against CarMV and analyzed the antiserum specificity with ELISA, Gel diffusion method, and Rapid Immunofilter Paper Assay (RIPA). From these results of the assay, RIPA method was simple and rapid for CarMV detection. We have established successfully the CarMV detection system. CarMV coat protein gene was amplified by RT-PCR with specific primers and sequencing analysis was done.

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.331-338
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• 2004

This study was conducted to obtain the virus free plants through meristem culture of carnation (Dianthus caryophillus). Four cultivars (Roland, Desio, Casha, Giant Gipsy) were collected for materials. The apical meristem 0.3-0.5mm in size was cultured on MS medium containing 3% sucrose, 0.9% agar at pH 5.8 with various plant growth regulators for 7 weeks. Among the cultivars, Giant Gipsy had a better response than other cultivars in shoot formation and reduced vitrification. Callus induction and shoot formation from the meristem culture were influenced by the various kinds of cytokine. Kinetin supplement was the most effective for shoot formation and NAA addition was good for callus induction among the treatments. Total 115 plantlets derived from apical meristem culture were checked for CarMV and CarRSV infection by ELISA test. Among them, 40 plantlets (34.8%) were infected with CarMV but not detected for CarRSV.

• Management & Information Systems Review
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• v.25
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• pp.83-114
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• 2008

We examine whether spinoffs improve long-run stock returns and analyze the factors of long run stock returns. The measures of long run stock returns are CAR(Cumulative Abnormal Returns) and BHAR(Buy and Hold Abnormal Returns). The expected factors of abnormal returns are methods of spinoffs, size, BV/MV, administrative costs, cashflow and Herfindahl index. We find that long-run returns of the case such as carve-out methods, small size, high BV/MV, low administrative costs, low cashflow and low Herfindahl index are larger than those of other cases. We show positive relationship between spinoffs and long-run stock returns(CAR and BHAR). The results supports spinoffs, as the methods of focusing on core business, are very usefulness of corporate restructuring.

• Korean journal of applied entomology
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• v.17 no.3 s.36
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• pp.131-138
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• 1978

A virus named Cymbidium mild mosaic virus(Cy MMV), was mechanically transmitted to Chenopodium amaranticolor from the leaves of Cymbidium with mild mosaic symptoms. The virus was cultured in C. amaranticolor, in which it produced local chlorotic and ring spots, followed by systemic vein clearing with distortion. CyMMV infected 7 out of 35 species of plants. In C. amaranticolor juice infectivity was lost by heating at $90^{\circ}C$ for 10 miuntes, and by aging at$20^{\circ}C$ for 60 days, and by diluting at $10^{-6}$ when bioassayed on C. amaranticolor. CyMMV was not transmitted by Myzus persicae. The virus was purified after clarification of homogenized C. amaranticolor leaf tissues with chloroform, by differential centrifugation followed by sucrose density gradient centrifugation. Electron microscopic examination of purified preparation showed spherical particles of 28nm in diameter. The UV absorption spectrum of purified preparation was typical of u nucleoprotein (max. at 261nm. min. at 243nm), and showed 260/280=1.72 and max/min=1.26. The value of the sedimentation coefficient of the virus was S20.w=126. In gel-diffusion tests, CyMMV antiserum reacted with CarMV, but not with any of four other viruses (BBWV, CRSV, CMV, TBRV) having similar particles and properties in vitro. In ultra-thin sections of CyMMV infected tissues, a large number of virus particles were found in the cytoplasm of mesophyll cells and in xylem vessels.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.16 no.4
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• pp.107-121
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• 2017

This study conducted a 3-stage survey and simulation experiment to identify the impact of vehicle platoons on car-following behavior of manually-driven vehicles. Vehicle maneuvering data obtained from driving simulations was statistically analyzed based on three measures including average speed, acceleration noise, and offset to represent the deviation of lateral movements. Results indicate that MV drivers tended to have psychological burden while driving in automated vehicle platooning environments, which resulted in different vehicle maneuvers. It is expected that the outcome of this study would be useful fundamentals in developing various traffic operations strategies for managing mixed traffic stream consisting of MVs and autonomous vehicles.

• The Plant Pathology Journal
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• v.18 no.5
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• pp.237-243
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• 2002

A survey of virus infection in garlic plants cultivated in Korea was conducted for three years. Most virus-infected garlic plants (Allium sativum) showed typical symptoms on the leaves such as yellow mosaic, stripes, and distortion. Through immunosorbent electron micro-scopy and RT-PCR analysis, the complex mixtures of viruses including garlic viruses of the genus Allerivirus, gaylic strain of Leek yellow stripe virus of the genus Potyvirus, and Garlic latent virus of the genus Carlavirus were identified in the virus-infected garlic plants. Among these viruses, Allexivirus was the most frequently detect-ed in the regions surveyed. Using sets of differential primers for Allexivirus genomes, two members of the genus were amplified and sequenced from the purified viruses. The deduced amino acid sequences for the coat proteins and the nucleic acid binding proteins of two viruses showed high homologies to Garlic virus A (CarV-A) and Garlic virus D (GarV-D) of Allekivirus. This is the first report of GarV-A and GarV-D in Korea. This suggests that Allexivirus in gavlic plants in Korea was mixed and varied. Phylogenetic analyses showed that the genus Allexivirus was diversi(ied by the processes of accumulation and evolution of viruses in garlic plants due to the long period of repeated vegetative propagation.