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Virus free Healthy plant production through Meristem culture in carnation (Dianthus caryophillus)  

정재훈 (남도대학 산림과학원 생물공학과)
김영선 (남도대학 약용자원원예개발과)
은종선 (전북대학 원예학과)
Publication Information
Korean Journal of Plant Resources / v.17, no.3, 2004 , pp. 331-338 More about this Journal
Abstract
This study was conducted to obtain the virus free plants through meristem culture of carnation (Dianthus caryophillus). Four cultivars (Roland, Desio, Casha, Giant Gipsy) were collected for materials. The apical meristem 0.3-0.5mm in size was cultured on MS medium containing 3% sucrose, 0.9% agar at pH 5.8 with various plant growth regulators for 7 weeks. Among the cultivars, Giant Gipsy had a better response than other cultivars in shoot formation and reduced vitrification. Callus induction and shoot formation from the meristem culture were influenced by the various kinds of cytokine. Kinetin supplement was the most effective for shoot formation and NAA addition was good for callus induction among the treatments. Total 115 plantlets derived from apical meristem culture were checked for CarMV and CarRSV infection by ELISA test. Among them, 40 plantlets (34.8%) were infected with CarMV but not detected for CarRSV.
Keywords
CarMV; CarRSV; Dianthus caryophillus; ELISA; meristem culture;
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