• Title/Summary/Keyword: Capsicum annuum L

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Plant Regeneration and Expression of Mouse Adenosine Deaminase Gene in Transgenic Hot Pepper (Capsicum annuum L.) Plants (형질전환된 고추( Capsicum annum L.) 식물체의 Mouse Adenosine Deaminas 유전자 발현)

  • 양덕춘;이계연;유영숙;최경화;임학태
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.1
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    • pp.37-41
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    • 1997

  • The in vitro regeneration and genetic transformation systems in hot pepper(Capsicum annuum L.) have not been routinely available, which has been a major limiting factor in the application of new genetic manipulations. An efficient procedure to regenerate whole pepper plants and to generate transgenic plants expressing a foreign gene was established. A relatively high frequency of plant regeneration was observed when hypocotyl and cotyledon explants were cultured on MS medium supplemented with NAA 0.1 mg/L plus zeatin 2.0 mg/L or IBA 10.0 mg/L plus BAP 1.0 mg/L. Addition of AgNO$_3$5 $\mu$M to these media improved the regeneration frequency up to 8%. For plant transformation, hypocotyl and cotyledon explants of hot pepper were precultured on shoot induction media without kanamycin added for 2 days, and then cocultured with Agrobacterium tumefaciens pDY183 for 2 days. Putative transformants were obtained from selection media containing 100 mg/L kanamycin sulfate and 500 mg/L carbenicillin. Putatively selected transformants were confirmed by amplification of selectable marker genes (ADA and NPT II) by polymerase chain reacion. Successful transcripts of ADA gene were detected by Northern blot analysis. Enzyme activity of ADA was also examined by spectrophotometric analysis, and expression of ADA gene in hot pepper suggests the potential application of ADA gene as a selectable marker in plants.

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Fruit Yield and Morphological Characters of Parental Cultivars and Intervarietal Hybrids of Capsicum annuum L. (고추의 친품종 및 품종간 잡종의 과실수량과 형태적 특성)

  • 조만현
    • Journal of Life Science
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    • v.6 no.4
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    • pp.219-226
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    • 1996

  • The fruit yield and morpholgical characters of F$_{1}$ hybrids between Capscium annuum L. 'California Wonder', four native Japanese cultivars, and three Korean F$_{1}$ cultuvars were evaluated and the results compared with parental cultivars. The F$_{1}$ hybrids obtained by crossing 'California Wonder' and native Japanese cultivars flowered 3 days earlier on average compared to parental cultivars. The yields of F$_{1}$ hydrids were higher than those of the parental cultivars, especially when one of the parents was 'California Wonder'. Also F$_{1}$ hybrid whose one parent was 'California Wonder' and 'Fushimi Amanaga' gave the highest fruit yield. The number of locules and seeds per fruit from of the 'California Wonder' hybrids whose one parent was 'California Wonder' was more than of the native Japanese cultivars. The plant height in F$_{1}$ hybrids obtained by crossing between 'California Wonder' and four native Japanese cultivars tended to be higher than that of the parental cultivars.

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Capsaicinoid Content in Fruits of Parental Cultivars and Intervarietal Hybrids of Capsicum annuum L. (고추의 친품종 및 품종간 잡종의 과실에 있어서 캡사이시노이드 함량)

  • 조만현
    • Journal of Life Science
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    • v.6 no.4
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    • pp.227-233
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    • 1996

  • The capsaicinoid content of F$_{1}$ hybrids between annuum L. 'California Wonder', four native Japanese cultivars, and three Korean F$_{1}$ cultivars were evaluated and the results compared with parental cultivars. The capsaicinoid content was higher in the placenta than in fruit flesh. The placenta of 'Yatsufusa', 'Taka no Tsume', 'Cheongyang', 'Sinhong', and Geoseong' were the most pungent cultivatrs, with capsaicinoid content of more than 1000mg/100g DW, while 'California Wonder', and 'Fushimi Amanaga' contained trace amounts. Capsaicinoid content in F$_{1}$ hybrids varied with reciprocal crossing. Capsaicinoid content also varied with fruit development, rapidly increasing in the fourth or fifth week after flowering, and thereafter slowly declined.

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