• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.123-123
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• 2003

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.122-122
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• 2003

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2006.10a
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• pp.108-108
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• 2006

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2006.10a
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• pp.111-112
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• 2006

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2004.10a
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• pp.83-84
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• 2004

• Journal of Embryo Transfer
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• v.21 no.1
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• pp.7-11
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• 2006

개 난자의 체외성숙과 체외수정을 통해 수정란을 획득하기 위하여 개 난소를 채취하여 난자의 형태, 난소의 채취시기 등이 체외성숙과 체외수정에 미치는 영향을 구명하기 위하여 수행하였다. 1. 난구세포 부착 및 미부착 난자를 48시간 배양했을 때 체외 성숙율은 $42.0{\pm}3.4%,\;24.4{\pm}4.1%$였다. 난구 세포 부착 난자의 체외 성숙율이 미부착 난자의 체외 성숙율보다 유의하게 높게 나타났다(p>0.05). 2. 난소를 휴지기, 난포기 및 황체기로 구분하여 난자를 회수하여 배양했을 때 체외 성숙율은 각각 35.6%, 50.0%, 31.1%였고 체외 발생율은 각각 2.2%, 20.0% 및 8.9%로서 난구 세포 부착 난자가 미부착 난자에 비해 유의한 체외 수정율을 나타냈다. 3. 난구세포 부착 및 미부착 난자를 이용하여 체외수정시켰을 때 체외 수정율과 체외 발생율은 각각 48.0%, 35.6% 및 22.5%, 13.3%로서 난구세포 부착 난자가 미부착 난자에 비해 유의하게 높게 나타났다.

• Journal of Embryo Transfer
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• v.21 no.1
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• pp.1-5
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• 2006

본 연구는 개 채취 정액의 동결후의 생존성과 신선 및 동결 정자의 capacitation, acrosome reaction과 생존성을 조사하고, 아울러 신선 및 동결 정액을 자연 발정 또는 발정 유기 암캐 에게 인공수정 후 임신율을 조사하였다. 개 채취 정액의 동결 융해 후의 생존성은 $64.5{\pm}2.30%$로서 신선 정액의 생존성에 비해 유의하게 낮게 나타났다(p<0.05). 신선 및 동결 정액의 capacitation, acrosome reaction 및 생존성은 각각 $52.5{\pm}4.5%,\;9.5{\pm}0.6%,\;68.8{\pm}4.5%$ 및 $16.2{\pm}3.2%,\;3.2{\pm}0.5%,\;24.5{\pm}2.5%$로 나타났다. 신선 및 동결 정액을 자연 발정 또는 발정을 유기한 암캐에 인공수정했을 때 임신율은 각각 50.0% 및 33.3%로서 동결 정액을 이용했을 때 임신율이 신선 정액에 비해 낮은 임신율을 나타냈다.

• Journal of Embryo Transfer
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• v.28 no.3
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• pp.237-241
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• 2013

It is generally accepted that chronic stress impairs female reproduction. It negatively affects ovarian function and the number of ovulated oocytes. Chronic stress lowers the number of retrieved oocytes. Ovarian follicular development is regulated by both pituitary-derived gonadotropins and intraovarian regulatory factors. The main corticosteroids are cortisol, cortisone, 11-deoxycortisol and corticosterone, cortisol being one of the most commonly used welfare and stress physiological indicator. In this study, we investigated the effect of cortisol level on progesterone patterns and ovulation in the dog. Cortisol and progesterone level of serum were analyzed by radioimmunoassay. The day of ovulation was considered as the day when serum progesterone concentration was 6.0~8.0 ng/ml. In vivo dog oocytes were collected by flushing oviducts of mixed-breed bitches at three days after ovulation. We classified dogs as having group 1 (cortisol level, 0 ${\leq}$ or < $2{\mu}g/dl$), group 2 (corisol level, 2 ${\leq}$ or < $4{\mu}g/dl$), group 3 (cortisol level, 4 ${\leq}$ or < $6{\mu}g/dl$) and group 4 (cortisol level, $6{\mu}g/dl$ ${\leq}$). The patterns of progesterone were not different in four cortisol groups. The average numbers of retrieved oocytes was not different in four cortisol groups. These results suggest that different cortisol levels on estrus dogs do not affect ovulation, number of ovulated oocytes and progesterone changes.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2004.10a
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• pp.49-50
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• 2004

• Journal of Embryo Transfer
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• v.17 no.2
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• pp.145-151
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• 2002

The aim of these experiments was to investigate in vitro nuclear maturation of canine oocyte collected from various stages of estrus cycles, Ovaries were obtained from 1 to 4 year-old mongrel bitch and minced for oocyte collection in phosphate buffered saline with 100 iu penicillin-G $m\ell$／sup -1／, 50 $\mu\textrm{g}$ streptomycin sulphate $m\ell$／sup -1／ and 0.1％ (w／v) polyvinyl alcohol. Cumulus-oocyte-complexes (COCs) were washed in HEPES buffered tissue culture medium (TCM)199 and in vitro matured in TCM-199 culture medium supplemented with sodium pyruvate 0.028mg/$m\ell$, L-glutamine 0.146mg／$m\ell$, penicillin G 10,000 IU／$m\ell$, streptomycin 0.031mg／$m\ell$ and 10％ (v／v) fatal calf serum. COCs were in vitro matured for 48～72 hrs at 39$^{\circ}C$ in humidified 5％ $CO_2$ in air atmosphere. In vitro matured oocytes were remove the cumulus cells using 0.2％ (v／v) hyaluronidase. After denuding, oocyte were placed in acetic acid : methanol : chlorform solusion (3:6 : 1.5 v／v) for 30 sec and acetic acid: ethanol(1:3 v／v) for 48hrs fixation. Nuclear maturation was classified to GV, GVBD, MI, MII and degenerate oocyte under microscopy after 1％ aceto-orcein stain. In vitro maturation rates at 48hrs were not significantly difference among the oocytes collected from different stage of estrus at 15.9％, 16.3％, 23.7％ and 18.2％ for anestrus, proestrus, estrus and diestrus. However, the oocytes maturation(36.6％) of collected from estrus ovaries were significantly different from oocytes derived from proesturs, diestrus and anestrus ovaries(30.8％, 17.5％ and 22.1％; p＜0.05). The overall in vitro maturation rates were significantly higher (p＜0.05) in 72hrs culture than 48hrs culture system. In summary, there was a tendency for higher in vitro maturation rates with the oocyte collected from estrus ovary than other stages of estrus. Also, for nuclear maturation, in vitro culture of oocyte for 72hrs was better than 48hrs culture.