• 한국임상수의학회지
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• 제28권3호
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• pp.307-309
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• 2011

1년령 암컷 요크셔테리어가 설사와 활력저하로 충북대학교 동물의료센터에 내원하였다. 혈액, 혈청화학 및 방사선 검사 결과로 환자는 복막염을 동반한 자궁축농증으로 진단하였다. 설사 원인 진단을 위한 분변 검사에서는 다수의 효모 증식이 관찰되었으며, 진균 배양 및 분자생물학적 동정으로 C. parapsilosis를 최종적으로 확인하였다. 이 보고는 개에서 C. albicans외에 C. parapsilosis도 장관 질환을 야기할 수 있음을 보여준다.

• KSBB Journal
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• 제13권1호
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• pp.6-12
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• 1998

In order to produce xylitol from hemicellulose hydrolysate which is widely used as a substrate, the development of strain such as catabolite derepressed mutant is required. After treatment of Candida sp. with EMS, GM-17 and PM-34 as catabolite derepressed mutant were isolated from Candida guilliermondii and Candida parapsilosis, respectively. Mutant GM-17 and PM-34 simultaneously assimilated xylose and glucose during the fermentation. The specific xylose reductase and xylitol dehydrogenase activities of mutant strains were also higher than those of wild strains in glucose medium and mixed medium of glucose and xylose. The xylitol productivity and yield of mutant GM-17 and PM-34 were improved as compared to the wild types in the mixed medium. The xylitol productivity and yield of mutant GM-17 were 0.09 g/L·hr and 0.56 g-xylitol/g-xylose, and those of mutant PM-34 were 0.21 g/L·hr and 0.52 g-xylitol/g-xylose in the mixed medium, respectively.

• Journal of Korean Neurosurgical Society
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• 제47권4호
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• pp.295-297
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• 2010

Candida is a relatively rare cause of spinal infections that commonly affects immunocompromised patients. A 70-year-old woman, who underwent a lumbar discectomy on L5-S1 two months earlier, was admitted to our department complaining of persistent back and leg pain. Magnetic resonance imaging showed irregular enhancing mass lesion in L5-S1 intervertebral space, suggest of pyogenic discitis with epidural abscess. The surgery was performed via retroperitoneal approach and the infected material at L5-S1 intervertebral space was removed. The histological examination of the specimen revealed chronic inflammation involving the bone and soft tissue, and a culture of the excised material was positive for Candida parapsilosis. The patient received intravenous fluconazole for 4 weeks after surgery and oral fluconazole 400 mg/day for 3 months after surgery. The patient made a full recovery with no symptoms 6 months after surgery. We present a rare case of spondylodiscitis after a lumbar discectomy due to Candida parapsilosis and discuss treatment option with a review of the literatures.

• 대한화학회지
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• 제55권2호
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• pp.256-261
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• 2011

Bis acetylated hybrid pyrazoles 을 합성하여 이들 화합물에 대해 녹는점, 원소분석, MS, FT-IR, one-dimensional $^1H,-$ 및 $^{13}C$-NMR로 분석하였다. 합성한 화합물들에 대해 in vitro 항균활성을 Candida sp. namely Candida albicans, Candida glabrata, Candida parapsilosis, Candida dubliniensis 및 Candida tropicali 균에 대해 수행하였다. Pyrazoles의 페닐고리에 작용기($-CH_3$, $-OCH_3$, -F, -Cl, 및 Br)가 있는 화합물은 Candida species에 대해서 강한 활성을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제19권1호
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• pp.65-71
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• 2009

Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols. However, when reused in multi-batch reaction, the catalytic efficiency and sustainability of non-growing cells usually decreased because of continuous consumption of required cofactors during the reaction process. A novel method for NADPH regeneration in cells was proposed by using pentose metabolism in microorganisms. Addition of D-xylose, L-arabinose, or D-ribose to the reaction significantly improved the conversion efficiency of deracemization of racemic 1-phenyl-1,2-ethanediol to (S)-isomer by Candida parapsilosis cells already used once, which afforded the product with high optical purity over 97%e.e. in high yield over 85% under an increased substrate concentration of 15 g/l. Compared with reactions without xylose, xylose added to multi-batch reactions had no influence on the activity of the enzyme catalyzing the key step in deracemization, but performed a promoting effect on the recovery of the metabolic activity of the non-growing cells with its consumption in each batch. The detection of activities of xylose reductase and xylitol dehydrogenase from cell-free extract of C. parapsilosis made xylose metabolism feasible in cells, and the depression of the pentose phosphate pathway inhibitor to this reaction further indicated that xylose facilitated the NADPH-required deracemization through the pentose phosphate pathway in C. parapsilosis. moreover, by investigating the cofactor pool, the xylose addition in reaction batches giving more NADPH, compared with those without xylose, suggested that the higher catalytic efficiency and sustainability of C. parapsilosis non-growing cells had resulted from xylose metabolism recycling NADPH for the deracemization.

• 한국미생물·생명공학회지
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• 제13권4호
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• pp.371-376
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• 1985

인삼추출물에서 분리한 Candide parapsilosis의 생육특성을 비교 검토한 결과 생육최적 pH는 6.0이었고 pH 4-7에서 정상적 생육을 보였다. 최적 Aw는 0.89이었고 배양조건에 따라 차이가 있어 전배양시에 Aw가 낮을 경우 최적 Aw도 낮아졌으며 이때 내삼투압성은 증가되었다. 일반적으로 배지의 Aw 0.995이상은 3$0^{\circ}C$, 0.98, 0.95, 0.92에서는 32$^{\circ}C$, 0.90이상에서는 34$^{\circ}C$에서 비증식속도가 가장 높았다. C. parapsilosis의 무기염류에 의한 생육저해는 양이온의 경우 L $i^{+}$>C $a^{2+}$>M $g^{2+}$>$Na^{+}$> $K^{+}$의 순이었고 음이온의 경우는 S $O_4$$^{2-}$ >N $O_3$$^{-}$>Cl의 순으로 나타났다.

• Journal of Mycology and Infection
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• 제23권4호
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• pp.105-110
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• 2018

Background: Otomycosis is a fungal infection that comprises 7~10% of outer ear infections. Although the occurrence is higher in humid climates, relatively few studies have investigated otomycosis occurrences in humid environments. While recurrent chronic otitis media discharge in the ear creates a milieu in which otomycosis is likely to occur, investigations of otomycosis co-occurring with chronic otitis media have been rare. Objective: To examine the characteristics of patients with otomycosis co-occurring with chronic otitis media and identify causative fungi. Methods: The study included 60 patients with chronic otitis media who presented typical otomycosis findings in the outer ear canal and the presence of fungi. Patients were treated in the department of otolaryngology, Daegu Catholic University Medical Center, between July 2011 and June 2018. Results: The mean patient age was 57.77 years, and our study included 20 men and 40 women (p=0.010). The lesion was on the right in 39 patients and on the left in 21 (p=0.020). Ear discharge was the most common chief complaint at diagnosis. Of the 54 patients over age 19, 10 had diabetes (18.5%). Aspergillus was causative in 29 patients and Candida in 31. Aspergillus niger was identified in 15 patients, Aspergillus sp. in 14, Candida parapsilosis in 12, Candida sp. in six, and Candida albicans in five. Conclusion: Otomycosis and chronic otitis media co-occurrences increase with age. The Aspergillus and Candida genera were similar in proportion. A. niger was the most common Aspergillus species, while C. parapsilosis was the most common Candida.

• Journal of Ginseng Research
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• 제13권1호
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• pp.66-70
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• 1989

홍미삼엑기스의 일부 성분이 내삼투압성 효모인 C. parapsilosis의 생육에 미치는 영향을 조사하기 위하여 홍미삼엑기스로부터 유기용매로 이행되는 일부 성분군을 분리 추출하여 그 조성을 조사하고 각 성분군이 C. parapsilosis의 증식에 미치는 영향을 조사하였다. 균증식은 헥산 가용성 물질(Fr.A)에 의하여 현저한 억제작용을 받았고 Fr.A와 조사포닌이 동시에 혼재하면 다소 회복되기는 하였으나 PGS(대조구) 배지에서 보다는 낮았다. 에텔 가용성 물질(Fr.B) 및 에틸아세테이트 가용성 물질(Fr.C)은 현저한 균증식 촉진효과가 있었고 Fr.B 혹은 Fr.c와 조사포닌이 동시에 혼재하면 세포 증식속도가 더욱 촉진되어 Fr.B와 조사포닌 그리고 Fr.C와 조사포닌은 상승적 촉진작용을 하였으며 셀룰로오스 투석막 외액(Fr.E)도 생육을 촉진하였으며 조사포닌과 함께 상승적 촉진작용을 하였다. 조사포닌은 단독으로 존재할 때 균생육에 큰 영향을 미치지 않았으나 다른 성분과 함께 상승적 촉진작용을 하는 특성이 있어 단일 ginenoside 수준에서의 균생육에 미치는 영향이 검토되어야 할 것으로 생각된다.

• 한국미생물·생명공학회지
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• 제24권4호
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• pp.507-511
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• 1996

Medium optimization for xylitol production from xylose by Candida parapsilosis ATCC 22019 mutant was performed. Effect of various nitrogen sources on xylitol production was investigatied. Of inorganic nitrogenous compounds, ammonium sulfate was effective for xylitol production and yeast extract was the most suitable orangic nitrogen nutrient for enhancement of xylitol production. Effect of inorganic salts such as KH$_{2}$PO$_{4}$ and MgSO$_{4}$-7H$_{2}$0 on xylitol production was also studied. Optimal medium was selected as xylose of 50 g/l, yeast extract of 5 g/l, (NH4$_{4}$)$_{2}$SO$_{4}$ of 5 g/l, KH$_{2}$PO$_{4}$ of 5 g/l, MgSO$_{4}$-7H$_{2}$O of 0.2 g/l. In a fermentor by using the optimal medium, a final xylitol concentration of 37 g/l could be obtained from 50 g/l of xylose with a xylitol yield of 74% and a xylitol productivity of 0.58 g/1-hr. At 300 g/l xylose, fermentation was also carried out and then a final xylitol concentration of 242 g/l was obtained at 272 hours. It was corresponding to xylitol yield of 80.7% and xylitol productivity of 0.58 g/1-hr.

• 한국식품과학회지
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• 제28권4호
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• pp.756-761
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• 1996

Candida parapsilosis KFCC 10875를 사용하여 acetic acid가 xylitol 생산에 미치는 영향을 조사하였다. 초기 acetic acid농도가 1.0 g/l까지는 acetic acid가 발효과정 중에 모두 소모되나, 3.0 g/l 이상의 농도에서는 일부만 소모되고 배지중의 존재하여 xylitol 발효에 영향을 주었다. 균체의 성장, xylose소비와 xylitol 생산은 acetic acid농도가 증가함에 따라서 감소하였다. 비 균체 증식속도와 비 기질 소비속도도 acetic acid가 증가할수록 감소하였다. 그러나, xylose에서 xylitol로의 전환수율과 비 생산속도는 acetic acid 농도가 1.0 g/l일 때까지는 최대값을 보여주었다. 그 이상의 acetic acid 농도에서는 xylitol의 전환수율과 비 생산속도가 감소하였다. Xylose로부터 xylitol 발효시 acetic acid의 저해정도는 pH에 영향을 받아 낮은 pH에서 균체의 증식, xylose의 소비 및 xylitol의 생성이 더 저해되었다.