• Title/Summary/Keyword: Camellia japonica L. seed cotyledons

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Adventitious Bud Induction and Plant Regeneration from Cotyledon Explants of Camellia japonica L. (동백 자엽절편으로부터 부정아 형성을 통한 식물체의 재분화)

  • Kim, Kwang-Soo;Hwang, Sung-Jin;Pyo, Byoung-Sik;Kim, Sun-Min
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.3
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    • pp.105-108
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    • 2005
  • Culture conditions for plant regeneration of Camellia japonica were achieved by organogenesis in explants of cotyledon. Seed cotyledons were cultured on MS medium containing various auxin, 2,4-D or NAA and cytokinins BA. The adventitious shoot buds were efficiently formed without embryogenesis on the basal region of cotyledon cultured on MS medium supplemented with $0.1\;{\mu}g/m{\ell}$ 2.4-D and $1\;{\mu}g/m{\ell}$ BA. Seed cotyledons could be used as a source of explants in experiments of genetic transformation of the genotypes evaluated for improving the efficiency of production of transgenic Camellia plants.