• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.175-175
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• 2017

To understand molecular mechanisms underlying adaptation of plant cells to saline stress and stress memory, we developed Arabidopsis callus suspension-cultured cells adapted to high salt. Adapted cells to high salt exhibited enhanced tolerance compared to control cells. Moreover, the salt tolerance of adapted cells was stably maintained even after the stress is relieved, indicating that the acquired salt tolerance of adapted cells was memorized. In order to characterize metabolic responses of plant cells during adaptation to high salt stress as well as stress memory, we compared metabolic profiles of salt-adapted and stress-memorized cells with control cells by using NMR spectroscopy. A principle component analysis showed clear metabolic discrimination among control, salt-adapted and stress-memorized cells. Compared with control cells, metabolites related to shikimate metabolism such as tyrosine, and flavonol glycosides, which are related to protective mechanism of plant against stresses were largely up-regulated in adapted cell lines. Moreover, coniferin, a precursor of lignin, was more abundant in salt-adapted cells than control cells. Cell morphology analysis using transmission electron microscopy indicated that cell wall thickness of salt-adapted cells was significantly induced compared to control cells. Consistently, salt adapted cells contained more lignin in their cell walls compared to control cells. The results provide new insight into mechanisms of plant adaptation to saline stress as well as stress memory in metabolic level.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.152-152
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• 2017

Plants exposed to environmental stress for long durations often can adapt to stress conditions with improved tolerance. Moreover this acquired tolerance to stress can be retained even after reverting to destressed growth conditions, which is known to stress memory. In these adaptation and stress memory processes, epigenetic regulation, such as DNA methylation and histone modifications play a key role. Here, we showed that regenerated rice plants from embryogenic callus exposed to gradually increasing NaCl concentrations (up to 120 mM NaCl) acquired salt tolerance and their enhanced tolerance are inherited to subsequent generations. The rice plants (R0) regenerated from rice callus under saline conditions were transplanted into normal paddy field and R1 seeds were harvested. These R1 seeds displayed higher germination rate on MS medium containing 100mM NaCl than wild-type. The callus derived from R1 seeds showed better growth than control callus on high salinity medium. And the salt-adapted R1 plants exhibited higher chlorophyll contents and also higher $K^+/Na^+$ ratio than wild-type rice under saline conditions. The results indicated that rice plants successfully adapted to saline growth conditions during regeneration on high salt medium and moreover this acquired tolerance to salt stress was inherited subsequent generation.

• 한국자원식물학회지
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• 제37권4호
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• pp.431-438
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• 2024

홉은 맥주 생산에서 풍미와 쓴 맛을 제공하며 방부제의 역할을 한다. 최근 수제 맥주 호황에 의해 외국의 홉 품종을 도입하여 국내 재배 및 생산을 도모하고 있으나, 효율적인 무병묘 생산에 있어 어려움을 겪고 있다. 이에 따라 본 연구는 홉(Humulus lupulus L.) Cascade 품종을 대상으로 조직배양 기술을 통한 효율적인 기내 증식 방법과 우량묘 생산기술을 개발하고자 수행되었다. 실험에 사용된 식물 생장조절제는 MS 배지에 auxin 계열 IAA와 cytokinin 계열의 2iP, zeatin, BAP, TDZ을 사용하였다. 식물 재료는 신초를 대상으로 정단에서 3마디를 제거하고 사용하였다. 홉 기내배양 시 재분화율은 IAA만 첨가한 조건에서 가장 높았으며, cytokinin을 함께 사용했을 때 보다 약 21% 높았다. 하지만 IAA 0.1 mg/L + BAP 1 mg/L 조건에서 재분화율이 약 91%로 우세하였다. 초장은 IAA 0.1 mg/L + BAP 1 mg/L를 사용하는 것이 유리하다. TDZ가 첨가된 배지에서 다경 유도가 이루어졌으며, control이나 IAA만 첨가된 조건의 경우 callus 형성이 이루어지지 않고 지상부와 뿌리 생장이 진행되었다. Cytokinin을 첨가한 배지에서 callus가 형성되며 무게가 증가하였다. 홉 조직배양묘의 순화 시 생존율을 높이기 위한 실험 결과, 배양병에 멸균된 상토를 넣고 상대습도를 100%로 맞추어 준 다음점진적으로 기내의 상대습도를 낮추어 가며 21일 이후 야외로 나갔을 때 90%의 생존율을 확인하였다. 이러한 연구 결과는 다른 홉 품종에도 적용하여 각 품종별로 적합한 배지 조성을 찾거나 callus 및 다경 유도를 통한 무병묘 증식이나 육종 연구 등 사용자의 필요에 따라 적합한 조직배양 배지 조성을 찾는데 도움이 될 것으로 판단된다.

• Journal of Ecology and Environment
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• 제46권3호
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• pp.218-242
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• 2022

Background: Promising specific growth regulators are employed in the tissue cultures of various bamboo species. Specific natural hardening mixtures support the acclimatization and adaptation of bamboo under protected cultivation. Results: The growth regulators like 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Naphthaleneacetic Acid (NAA), Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin, Gelrite, Benzyl Adenine (BA), Indole Butyric Acid (IBA), Coumarin, Putrescine, Gibberellic acid (GA₃), Indole Acetic Acid (IAA) has been widely used for callus induction, root regeneration and imposing plant regeneration in various species of bamboo such as Bambusa spp. and Dendrocalamus spp. Different combinations of growth regulators and phytohormones have been used for regenerating some of the major bamboo species. Natural hardening materials such as cocopeat, vermicompost, perlite, cow dung, farmyard manure, compost, soil, garden soil, and humus soil have been recommended for the acclimatization and adaptation of bamboo species. Standard combinations of growth regulators and hardening mixtures have imposed tissue culture, acclimatization, and adaptation in major bamboo species. Conclusions: Bamboo contributes to soil fertility improvement and stabilization of the environment. Bamboo species are also involved in managing the biogeochemical cycle and have immense potential for carbon sequestration and human use. This paper aims to review the various growth regulators, natural mixtures, and defined media involved in regenerating major bamboo species through in vitro propagation. In addition, the ecological benefits of safeguarding the environment are also briefly discussed.

• 한국식품영양과학회지
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• 제27권4호
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• pp.698-704
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• 1998

This study was carried out to investigate the effects of diet of Korean safflower(Carthamus tinctorious L.) seed powder on bone tissue during the recovery of rib-fracture in rats. Male Sprague-Dawley rats of 10 weeks old, weighing 370$\pm$5g, were divided into two groups including the control group(C group, AIN-76 semipurified diet) and safflower seed group(S group, AIN-76 semipurified diet+10% safflower seed powder) and were fed experimental diets for 12 days after adaptation period. After this period, the 9th right rib was fractured surgically and sham-operation was also performed. Rats were fed with experimental diets for up to 30 more days after rib-fracture. The degree of bone repair was evaluated during the recovery period at the 8th, 11th, 16th, 21st, 30th days after the surgical operation by microscopic observation of the fractured rib tissue. In callus formation, the portion of hyaline cartilage was noticably higher in S group than C group. The intracatilagenous ossification was observed at the 8th day in S group, but at 11th day in C group. The intramembranous ossification in callus was widely found over the 8th day to the 11th day in S group, but it was shown over the 11th day to the 16th day in C group. Bone resorption was also occured more rapidly in S group as indicated by large numbers of osteoclasts observed. At the 30th day, most of trabecular bones were disappeared in S group, whereas still shwon in C group over wide ranges of fractured ribs. These results imply that the supplementation of Korean safflower seed powder influences in the recovery of bone fracture by accelerating the process of bone repair.