• 한국수산과학회지
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• 제34권4호
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• pp.384-388
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• 2001

바다방석고둥을 효율적으로 이용하기 위한 기초 연구로 바다방석고둥의 어획지별 (제주시, 서귀포시, 한림읍 및 성산읍 연안)위생적, 영양적, 맛 및 관능적 특성과 같은 식품성분 특성에 대하여 살펴보았다. 바다방석고둥은 수은, 납 및 카드뮴과 같은 중금속이 검출되지 않았거나 흔적량에 불과하여 식품소재로 사용하여도 중금속적인 면에서는 안전하다고 판단되었다. 바다방석고둥의 엑스분 질소함량은 $343.0\~385.4mg/100g$이었다. 구성아미노산함량은 $12.5\~13.2g/100g$이었고, 함량이 많은 것으로는 glutamic acid, proline 및 aspartic acid 등이었으며, 곡류 제한아미노산인 lysine의 함량도 많았다. 그리고, 무기질은 나트륨, 칼슘, 칼륨 및 마그네슘 등의 함량이 많았다. 바다방석고둥의 지방산조성은 필수지방산이거나 건강기능성이 인정되는 20:4n-6, 20:5n-3 및 22:5n-3 등과 같은 고도불포화산이 주요 지방산이었으나, 지질함량이 깎아 지방산에 의한 건강 기능성을 기대하기는 어렵다고 판단되었다. 이상의 결과로 미루어 보아 바다방석고둥은 어획지에 따른 차이가 거의 없어, 이들을 가공원료로 사용하는 경우 어획지역에 따른 가공품의 품질 차이는 없으리라고 판단되었다.

• 한국약용작물학회지
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• 제20권5호
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• pp.359-364
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• 2012

This study were carried out to find bolting response of cultivation in different regions and to isolate FLC (FLOWERING LOCUS C) homologs in Angelica gigas Nakai. The mean temperature of different regions, ordering in altitude, were as follows: 100 m > 350 m > 530 m > 700 m. The largest amount of rainfall was occurred in the region of 350 m while the longest time of sunshine was occurred in the region of 100 m. The content of soil chemical properties in regions showed pH 6.2 ~ 7.4, T-N 0.17 ~ 26, organic mater $1{\sim}32gkg^{-1}$, $P_2O_5$ ${151{\sim}664_{mgkg}}^{-1}$, exchangeable potassium and calcium and magnesium were 0.78 ~ 1.15, 3.9 ~ 10.0, ${0.7{\sim}3.2_{cmol}}^{+kg-1}$. L5 line of A. gigas was occurred in bolting at all regions, but the bolting ratio was 60.0% in 700 m region with non-mulching treatment. Manchu of A. gigas was not occurred in bolting at all regions. The accumulation bolting ratio of L5 line by non-mulching was higher than that of mulching as 90.4% and 72.8% in 100 m region. The MADS-box transcription factor FLC is one of the well-known examples as a strong floral repressor. We decided to isolate FLC homologs from A. gigas as a starting point of flowering mechanism research of this plant. We have isolated two RT-PCR products which showed very high amino acid sequence homology to Arabidopsis FLC.

• 한국광물학회지
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• 제17권3호
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• pp.277-288
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• 2004

알칼리-실리카반응은 시멘트 내의 알칼리와 화학적으로 불안정한 반응성 골재와의 화학반응으로서, 그 결과 콘크리트의 팽창과 균열을 발생하는 작용이다 본 연구에서는 우수한 포조란반응 특성을 나타내는 새로운 광물혼화재로 부각되고 있는 메타카오린의 알칼리-실리카반응 억제 효과에 대하여 연구하였다. 다양한 치환율로 메타카오린을 혼합한 모르타르 공시체를 제작하여, 알칼리-실리카 반응성 시험(ASTM C 1260), 압축강도 시험 및 유동성 시험을 실시하고, 시멘트 수화물에서의 구성성분 변화에 대한 XRD 정량분석을 실시하였다. 그 결과, 메타카오린의 혼합은 시멘트 페이스트내의 가용 포트랜다이트의 함량을 급속히 감소시키는 빠른 포조란반응 및 수화반응 특성을 나타내어, 알칼리-실리카 반응에 의한 팽창을 억제하고 우수한 압축강도를 발현하는 것으로 나타났다. 시멘트에 메타카오린의 혼합에 의한 알칼리-실리카 반응에 의한 팽창억제는 치환율 15% 이상, 즉 시멘트 페이스 내의 가용 포트랜다이트 함량이 약 10% 이하가 될 경우 효과적이다. 메타카오린의 혼합에 의한 알칼리-실리카 반응에 의한 팽창 억제는 유해성이 높은 알칼리-칼슘-실리카 겔의 형성이 억제된 결과와 포조란 효과에 의한 치밀하고 균질한 시멘트 페이스트 형성으로 인한 알칼리 용액의 침투가 억제된 결과에 의한 것으로 생각된다. 15% 이상의 메타카오린의 혼합은 보통의 모르타르보다 높은 초기강도를 발현하였으며, 후기강도는 전 치환율 범위에서 보통의 모르타르 이상의 아주 우수한 강도를 나타내었다. 강도발현 특징은 메타카오린에 의한 빠른 포조란반응 및 수화특성을 반영하고 있다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제31권4호
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• pp.287-293
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• 2009

Long-term treatment with glucocorticoid leads to the development of osteoporosis and osteonecrosis. In contrast to the marked inhibitory effect of pharmacological doses of glucocorticoids on bone formation, the relationship between physiological concentrations of glucocorticoids and osteoprogenitor cell proliferation and phenotypes has not been elucidated yet. In addition, the effects of dexamethasone treatment on the proliferation and osteoblastic differentiation of osteoprogenitor cells are also controversial. The purpose of this study was to examine the effects of dexamethasone on the proliferation and osteoblastic differentiation of periosteal-derived cells. Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the cells were further cultured for 21 days in the osteogenic induction medium with different dexamethasone concentrations of 0, 10, and 100 nM. The proliferation and osteoblastic phenotypes of periosteal-derived cells were promoted in dexamethasone-treated cells than in untreated cells. Among the dexamethasone-treated cells, cell proliferation was slightly greater in 10 nM dexamethasone-treated cells than in 100 nM dexamethasone-treated cells. Histochemical staining and the bioactivity of alkaline phosphatase (ALP) were higher in 100 nM dexamethasone-treated cells than in 10 nM dexamethasone-treated cells. Similarly, von Kossa-positive mineralization nodules and calcium content were also more evident in 100 nM dexamethasone-treated cells than in 10 nM dexamethasone-treated cells. These results suggest that dexamethasone enhances the in vitro osteoblastic differentiation of periosteal-derived cells. The present study also demonstrates that higher dexamethasone concentrations reduce the in vitro proliferation of periosteal-derived cells.

• 대한치과교정학회지
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• 제38권6호
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• pp.407-415
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• 2008

본 연구의 목적은 치간삭제 후 불소 및 수산화인회석 페이스트의 치면 도포에 대한 법랑질 표면의 결정구조 및 재광화를 평가하고자 하였다. 치간삭제 후 1.23% 인산화 불소 및 10% 수산화인회석 페이스트를 치면에 도포한 후 법랑질 표면의 결정구조 변화를 주사전자현미경으로 관찰하고, 법랑질 표면의 칼슘, 인, 불소 함량 등의 변화를 energy dispersive X-ray spectroscopy (ESD)로 분석하였다. 실험결과 10% 수산화인회석 페이스트 도포한 군에서 칼슘함량이 증가하였고 (p < 0.01), 치간 삭제 후 거칠었던 법랑질 표면이 매끄럽고 부드러워졌으며 법랑질 결정구조가 작고 치밀해짐을 관찰할 수 있었다. 이상의 결과는 치간삭제 후 불소 및 수산화인회식 도포가 치면 결정구조 및 재광화에 유리하다는 것을 의미한다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제35권5호
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• pp.294-298
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• 2009

Purpose: The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periosteal-derived cells. Materials and methods: Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and $\beta$-glycerophosphate. Results: The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells. Conclusion: These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.

• 농업과학연구
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• 제6권2호
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• pp.185-191
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• 1979

시멘트의 응결(凝結) 경화촉진제(硬化促進劑)로 사용(使用)되는 염화(鹽化)칼슘 첨가량(添加量)이 mortar의 강도(强度)에 미치는 영향(影響)을 연구(硏究)하기 위하여, 보통 mortar를 경화(硬化)시킨 경우와 $CaCl_2$을 1.0%. 2.0%, 3.0% 첨가(添加)하여 경화(硬化)시킨 경우의 압축강도(壓縮强度)와 인장강도(引張强度)를 배합비별(配合比別), 재영별(材令別)로 구분(區分)하여 비교시험(比較試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 압축강도(壓縮强度)는 염화(鹽化)칼슘을 첨가(添加)한 몰타르가 보통(普通) 몰타르보다 크게 나타났으며, 2.0% 첨가(添加)했을 때 최대강도(最大强度)를 나타냈다. 2.인장강도(引張强度)는 7일(日) 강도(强度)에서 염화(鹽化)칼슘을 첨가(添加)한 몰타르가 보통(普通)몰타르보다 높은 강도(强度)를 나타냈으나, 28일(日) 강도(强度)에서는 1.0% 첨가(添加)한 몰타르가 최대강도(最大强度)를 나타냈고, 2.0% 이상(以上) 첨가(添加)한 경우는 보통(普通)몰타르보다 적게 나타났다. 3. 염화(鹽化)칼슘 첨가량(添加量)이 3.0%이상(以上)일 때는 급결현상(急結現象)이 나타났으며 이로 인(因)한 균열 때문에 강도(强度)는 저하(低下)되는 것으로 사료(思料)된다. 4. 부배합(富配合)에서는 압축(壓縮) 및 인장강도(引張强度)가 재령(材令) 7일(日) 이전(以前)에는 강도증진(强度增進)에 효과(效果)가 컸고 재령(材令) 7일(日) 이후(以後)에는 큰 차이가 없었으므로, 염화(鹽化)칼슘을 1.0%~2.0% 첨가(添加)하여 사용(使用)하면 동기공사(冬期工事)에서 초기강도증진(增初期强度進)을 기(期) 할 수 있을 것으로 생각된다.

• 한국세라믹학회지
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• 제39권5호
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• pp.438-445
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• 2002

본 연구에서는 $PbO-TiO_2-SiO_2-BaO-ZnO-Al_2O_3-CaO-B_2O_3-Bi_2O_3-MgO$계의 유리를 $1,400{\circ}C$에서 용융시킨 후, 급랭, 분쇄하여 glass frit을 제조하였다. Glass frit 분말을 일축가압성형한 후 $750~1,000{\circ}C$의 온도범위에서 2시간 동안 소성 및 결정화 하였다. Glass frit의 결정화는 $750{\circ}$ 전후의 온도에서 시작되었고, 저온에서의 주된 결정상은 $Al_2O_3$와 hexagonal celsian($BaAl_2Si_2O_8$)이었다. 소성온도가 높아지면서 monoclinic celsian, $ZnAl_2O_4,\;Zn_2SiO_4,\;CaTi(SiO_4)O,\;TiO_24 등이 주된 결정상으로 나타났고, 특히 celsian은 hexagonal에서 monoclinic으로 상전이가 발생하였다. 그리고 15wt%의 PbO를 첨가한 glass frit에만 $PbTiO_3-CaTiO_3$ 고용체가 나타났다. 1MHz 대역에서 유전특성을 살펴본 결과 유전상수는 11~16이었고, 유전손실은 0.020 미만이었다. 그러나 15wt%의 PbO를 첨가한 glass frit의 경우는 $PbTiO_3-CaTiO_3$ 고용체 결정상의 존재로 유전상수가 17~26으로 높았고, 유전손실은 0.010~0.015로서 다른 조성들에 비하여 우수한 특성을 나타내었다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제31권6호
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• pp.478-484
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• 2009

Three-dimensional porous scaffolds play an important role in tissue engineering strategies. They provide a void volume in which vascularization, new tissue formation, and remodeling can occur. Like any grafted materials, the ideal scaffold for bone tissue engineering should be biocompatible without causing an inflammatory response. It should also possess biodegradability, which provides a suitable three-dimensional environment for the cell function together with the capacity for gradual resorption and replacement by host bone tissue. Various scaffolds have already been developed for bone tissue engineering applications, including naturally derived materials, bioceramics, and synthetic polymers. The advantages of biodegradable synthetic polymers include the ability to tailor specific functions. The purpose of this study was to examine the osteogenic activity of periosteal-derived cells in a polydioxanone/pluronic F127 scaffold. Periosteal-derived cells were successfully differentiated into osteoblasts in the polydioxanone/pluronic F127 scaffold. ALP activity showed its peak level at 2 weeks of culture, followed by decreased activity during the culture period. Similar to biochemical data, the level of ALP mRNA in the periosteal-derived cells was also largely elevated at 2 weeks of culture. The level of osteocalcin mRNA was gradually increased during entire culture period. Calcium content was detactable at 1 week and increased in a time-dependent manner up to the entire duration of culture. Our results suggest that polydioxanone/pluronic F127 could be a suitable scaffold of periosteal-derived cells for bone tissue engineering.

• Asian-Australasian Journal of Animal Sciences
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• 제14권4호
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• pp.515-524
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• 2001

Two experiments using growing and finishing pigs (Landrace$\times$Duroc$\times$Yorkshire) were conducted to determine the effects of different levels of vitamins and trace minerals (VTM) on growth performance, nutrient digestibility, carcass characteristics and meat quality of growing-finishing pigs, and to evaluate the suitability of vitamin-mineral levels commonly used in the swine industry in Korea. A total of 120 three crossbred (Landrace$\times$Duroc$\times$Yorkshire) growing (Experiment I: 20.90 0.44 kg average initial body weight) and finishing (Experiment II: 53.55 0.97 kg average initial body weight) were used in 6 and 9 weeks feeding trials, respectively. Pigs were allotted on the basis of sex and weight to 5 treatments with 6 replications per treatment with 4 pigs per pen in a completely randomized block design. Treatments were: 1) control, 2) fat soluble vitamin 200% vs. control, 3) water soluble vitamin 200% vs. control, 4) trace mineral 50% vs. control, 5) trace mineral 200% vs. control. In experiment I, during the overall experimental period (0 to 6 weeks, 21 to 54 kg body weight), ADG of the 200% trace mineral supplemented group was significantly higher than that of the 50% trace mineral supplemented group. There were no significant differences among other the treatments. Digestibility of crude protein was lowest in mineral 50% supplemented group (p<0.05). Calcium digestibility was significantly higher in the 200% fat soluble vitamin supplemented group than in the other treatments (p<0.05). In experiment II, during the overall experimental period (0 to 9 weeks, 54 to 106 kg body weight), growth performance was not significantly affected by dietary vitamin and trace mineral levels. However, increasing level of water soluble vitamins at the level of 200% compared to control had a tendency to improve the overall growth performance. Overall carcass characteristics except for carcass length did not differ among pigs fed the dietary treatments. Loin eye area, pH, drip loss and shear force of meat were not affected by dietary vitamin and trace mineral levels. There was a trend for less fat content (%) in pork when the level of vitamin and trace mineral was increased, but the difference was not significant. Flavor score was the lowest in control and highest in the 200% fat soluble vitamin supplemented group (p<0.05). Juiciness of muscle was lower in the 200% fat soluble vitamin supplemented group than other dietary treatments, except for trace mineral 50% supplemented group (p<0.05). Based on these results, it is suggested that "typical" commercial levels of vitamin and trace minerals used by feed companies in Korea are sufficient to meet requirement for the maximum growth of growing-finishing pigs. Our results suggests that a reduction in trace mineral levels in commercial diets could be considered to reduce feed cost and nutrient excretion with economic and environmental benefits.