Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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VEGF-RELATED AUTOCRINE GROWTH IN PERIOSTEAL-DERIVED CELLS

골막기원세포에서 발현되는 혈관내피세포성장인자 관련 자가성장

  • Park, Bong-Wook (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21)) ;
  • Lee, Seong-Gyun (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21)) ;
  • Hah, Young-Sool (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Kim, Deok-Ryong (Department of Biochemistry, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21)) ;
  • Cho, Yeong-Cheol (Department of Oral and Maxillofacial Surgery, College of Medicine, Ulsan University) ;
  • Sung, Iel-Yong (Department of Oral and Maxillofacial Surgery, College of Medicine, Ulsan University) ;
  • Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Jong-Ryoul (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Byun, June-Ho (Department of Oral and Maxillofacial Surgery, Gyeongsang National University School of Medicine and Institute of Health Sciences, Biomedical Center (BK21))
  • 박봉욱 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원, 의생명과학사업단 (BK21)) ;
  • 이성균 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원, 의생명과학사업단 (BK21)) ;
  • 하영술 (경상대학교병원 임상의학연구소) ;
  • 김덕룡 (경상대학교 의학전문대학원 생화학교실, 경상대학교 건강과학연구원, 의생명과학사업단 (BK21)) ;
  • 조영철 (울산대학교 의과대학 구강악안면외과학교실) ;
  • 성일용 (울산대학교 의과대학 구강악안면외과학교실) ;
  • 김욱규 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김종렬 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 변준호 (경상대학교 의학전문대학원 구강악안면외과학교실, 경상대학교 건강과학연구원, 의생명과학사업단 (BK21))
  • Published : 2009.10.30
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Abstract

Purpose: The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periosteal-derived cells. Materials and methods: Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and $\beta$-glycerophosphate. Results: The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells. Conclusion: These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.

Keywords

References

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