• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.2
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• pp.109-115
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• 1993

This study was designed to investigate the effects of dietary protein and calcium levels on Ca, Fe, Cu, Zn and Mg accumulation of the tissue of the Pb-administered rats. Male Sprague-Dawley rats were assigned to a 3$\times$3$\times$2 factorial design with 3 levels of protein (40%, 15%, 6%), 3 levels of Ca (1.2%, 0.6%, 0.12%) and 2 feeding periods (3 and 7 weeks). The control group was included separately. The rats were exposed to the drinking water containing 2,000ppm of lead. Calcium contents in serum, liver and femur were decreased with Pb administration. Calcium contents in serum and femur were reduced with dietary protein and Ca levels. Level of serum Fe showed no significant difference with Pb administration in the rats fed the high Ca diet. Iron content in liver was not affected by the lead when the rats fed the diet containing high protein and Ca. Level of serum Cu was lower in the Pb added groups than in the control group and tended to be reduced with decreasing dietary protein and Ca levels. Copper content in liver was not affected by the lead when the rats fed the high Ca diet. Level of serum Zn was decreased in the low protein-low Ca group. Magnesium content in serum was decreased with Pb administration when the rats fed the diet containing low protein and Ca. However, magnesium content in liver was reduced with Pb administration and affected only by dietary protein level.

• The Korean Journal of Physiology and Pharmacology
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• v.3 no.1
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• pp.101-117
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• 1999

Amyotrophic lateral sclerosis (ALS) is a degenerative neuromuscular disease of unknown etiology in which the upper and lower motor neurons are progressively destroyed. Recent evidences support the role of autoimmune mechanisms in the pathogenesis of ALS. This study investigated the effects of sera from ALS patients on neuromuscular transmission in phrenic nerve-hemidiaphragm preparations and on calcium currents of single isolated dorsal root ganglion (DRG) cells in mice. Mice were injected with either control sera from healthy adults or ALS sera from 18 patients with ALS of sporadic form, for three days. Miniature end plate potential (MEPP) and nerve-evoked end plate potential (EPP) were measured using intracellular recording technique and the quantal content was determined. Single isolated DRG cells were voltage-clamped with the whole-cell configuration and membrane currents were recorded. Sera from 14 of 18 ALS patients caused a significant increase in MEPP frequency in normal Ringer's solution $(4.62{\pm}0.14\;Hz)$ compared with the control $(2.18{\pm}0.15\;Hz).$ In a high $Mg^{2+}/low\;Ca^{2+}$ solution, sera from 13 of 18 ALS patients caused a significant increase in MEPP frequency, from $2.18{\pm}0.31$ Hz to $6.09{\pm}0.38$ Hz. Sera from 11 of 18 patients produced a significant increase of nerve-evoked EPP amplitude, from $0.92{\pm}0.05$ mV to $1.30{\pm}0.04$ mV, while the other seven ALS sera did not alter EPP amplitude. In the ALS group, EPP quantal content was also elevated by the sera of 14 patients (from $1.49{\pm}0.07$ to $2.35{\pm}0.07).$ MEPP frequency and amplitude in wobbler mouse were $4.03{\pm}0.53$ Hz and $1.37{\pm}0.18$ mV, respectively, which were significantly higher than those of wobbler controls (wobblers without the symptoms of wobbler). Sera from ALS patients significantly reduced HVA calcium currents of DRG cells to 42.7% at -10 mV. Furthermore, the inactivation curve shifted to more negative potentials with its half-inactivation potential changed by 6.98 mV. There were, however, significant changes neither in the reversal potential of $I_{Ca}$ nor in the I-V curve. From these results it was concluded that: 1) The serum factors of sporadic ALS patients increase neuromuscular transmission and can alter motor nerve terminal presynaptic function. This suggests that ALS serum factors may play an important role in the early stage of ALS, and 2) Calcium currents in DRG cells were reduced and rapidly inactivated by ALS sera, suggesting that in these cells, ALS serum factors may exert interaction with the calcium channel.

• The Journal of Natural Sciences
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• v.8 no.2
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• pp.175-179
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• 1996

The fruit soluble solids and acidity decreased during storage regardless of fruit size, fruit firmness decreased also, and the decreasing rate of the firmness in large fruit was higher than small. The ethylene production of fruit was increased during storage, and there was the peak of fruit respiration rate at 2 and 6 weeks after harvest. The calcium and magnesium content was the highest in peel, the lowest in flesh, and intermediate in core. Especially, the calcium content in the outer flesh was lower than in the inner flesh, and the magnesium content in the middle part of flesh was lower than in the other parts. The Ca/Mg ratio in the inner flesh and the K/Ca ratio in the outer flesh were higher than in the other parts.

• Journal of Food Hygiene and Safety
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• v.13 no.3
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• pp.258-267
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• 1998

The acetaminophen (AP AP), an antipyretic and analgesic agent, induces the hepatotoxicity by increasing influx of calcium and destabilizing the cellular membrane which can be caused by N-acetyl-p-benzoquinoneimine generated by cytochrome P-450 (CYF-450) when it is overdosed. Diltiazem (DIL), a calcium channel blocking agent, has been known to suppress the CYF-450 activities. To study the effect of DIL in APAP treated rats, the serum biotransformational enzyme analyses and the liver histopathologic examination were conducted on the rats which had been administered DIL at 3, 6, 9 and 12 hours after the 3,000 mg/kg of APAP administration. Following a single dose of DIL administered 12 hours after AP AP administration, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, malondialdehyde and calcium contents of liver and microsome were significantly reduced. Glutathione S-transferase (GST) activity was significantly increased. Histopathologic studies showed that DIL had prevented the development of centrilobular necrosis induced by AP AP in liver tissue. Our results suggested that diltiazem could inhibit the formation of free radical and the influx of calcium and could increase GST activity. Therefore, diltiazem can be administered at the time of 12 hours after overdosed AP AP to diminish the liver damage.

• Korean Journal of Veterinary Research
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• v.33 no.3
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• pp.493-505
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• 1993

Present experiments were undertaken in order to clarify the clinico-pathological characteristics of lead poisoning in goats. Twenty goats were divided into three experimental groups(A, B and C) and a control(D). The three experimental groups received diets contaminated artificially with 10(A group), 200(B group) and $1,000(C group){\mu}g/g$ of lead for 70 days, respectively. The control group received normal diets. Hair samples collected one or two weeks interval were examed for the lead content. On day 70. all the animals were necropsied and various organs were examined pathoanatomically. In addition, various organs were analyzed for lead, zine, copper, iron and calcium content by atomic absorption spectrophotometry. From these experiments following results were obtained ; The lead content of hair was significantly increased from the $42^{nd}$, $21^{st}$ or $14^{th}$ day in all the three groups, which showed significant correlation with lead content of liver and spleen. The lead content of various organs including kidney, liver, spleen, ileum, bone and abomasum were significantly increased in accordance with the lead contamination. The increase was most prominent in abomasal wall. The zinc and copper content of liver, brain, ileum, skin, kidney, lung or muscle were increased in B and C groups whereas the iron and calcium content showed no significant change. Pathoanatomically, degeneration or necrosis of proximal convoluted tubules of kidney, peripheral necrosis of liver, fundic gland necrosis of abomasum and cerebral edema were observed in B and C groups.

• Proceedings of the Korean Geotechical Society Conference
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• 1999.03a
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• pp.505-512
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• 1999

Soft marine clay deposits pose several foundation problems. Generally, lime stabilization is used worldwide for solidifying of soft marine clay deposits. In this paper, a series of laboratory tests were conducted to verify clay-lime reaction. A clay was collected from Pusan, which was mixed with various quantities of quick lime and slaked lime. Various compounds produced by clay-lime reaction were identified by X-ray diffraction analysis. The physico-chemical properties of the clay were also investigated. Compounds such as calcium silicate hydrate (CSH), calcium aluminate hydrate (CAH), calcium aluminate (CA), hillebrandite, and gehlenite were identified. It is likely that such compounds were mainly produced by pozzolanic reaction. Based on the change of physico-chemical properties obtained by the reaction, the water content was considerably decreased when lime was added to the clay. In addition, unconfined strength was increased. In the other hand, quick lime was more effective than slaked lime in decreasing and increasing of the water content and unconfined strength, respectively. Fewer cracks were produced when the clay was mixed with quick lime. It is suggested that these beneficial changes produced by the mixing of the clay and lime depend on the properties of compounds obtained by chemical reaction.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.1
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• pp.49-55
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• 1999

The effect of fat supplementation of high calcium (Ca) diets on the performance, intestinal pH, body composition and size and weight of organs in growing chickens were investigated in two experiments. Growing chickens tolerated a high dietary level of Ca (22.5 vs 12.1 g/kg) in the presence of 6.3 g/kg of available phosphorus without any significant effect on performance. Intestinal pH was significantly increased by the addition of excess Ca and fat which probably created the right pH for the formation of insoluble Ca soaps. Excess dietary Ca increased carcass linoleic acid concentration at the expense of palmitic and stearic acid contents, whilst the addition of sunflower oil (80 g/kg diet) to the diet increased carcass linoleic acid concentration at the expense of palmitic acid content of the carcass. Intestinal and visceral organ size and weight were not influenced by excess Ca or fat. However, there was a non significant increase in the intestinal dry weight per unit of length caused by excess dietary Ca. It was concluded that excess dietary Ca of 22.5 g/kg did not significantly influence the performance of meat chickens. However, excess Ca increased intestinal pH and altered carcass fatty acid composition. Fat supplementation did not alter intestinal pH with high Ca diets. Excess dietary fat altered carcass fatty acid composition and reduced protein content. Intestinal and visceral organ size and weights were not influenced by excess dietary levels of Ca of fat.

• Journal of Nutrition and Health
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• v.22 no.3
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• pp.149-158
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• 1989

To investigate the nutritional quality of packaged meals produced by packaged meal manufactures, a nutritional evaluation was conduced. Samples examined in this study were 95 Products which composed of 86 Doraks, 7 Kimplabs and 2 Yubuchopabs Produced in packaged meal manufactures in Seoul City and Kungki-do province The distribution of food groups showed that amounts of animal foods were abound, but amounts of green vegetables, limited in Dosirak comparing to one meal RDA levels. Amounts of meats, fishes and shells are increased more than amounts of egged and legumina with increase of price of Dosirak. The total kinds of food and the amounts of certain food in Kimpab and Yubuchopab were lower than Dosirak. The energy and protein contents of Dosirak were found to be higher than one meal RDA levels, with exception of protein content in Dosirak of the price 1000 won. The contents of energy and protein in Kimpab and Yubuchopab are less than one meal RDA levels. Calcium and iron contents in Dosirak, and calcium content in Yubuchopab were present in excess compared to the one meal RDA. However, In Kimpab, calcium and iron contents were lower than the one meal RDA. On the whole, vitamin contents of Dosirak were sufficient whereas vitamin contents of Kimpab and Yubuchopab were lower than the one meal RDA. Number of side dishes used in Dosirak were 6-23 items depending on prices. Guidelines were suggested for effective nutritional quality control of packaged meals.

• Journal of Nutrition and Health
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• v.26 no.2
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• pp.154-154
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• 1993

A factorial experiment was conducted to determine the influence of phytate(0 or 10g/kg diet) and calcium (Ca)(3 or 10g/kg diet) intakes on Ca, P and Zn metabolism by growing female rats. Food intake and weight were similar for the all groups, however, phytate ingestion for six weeks depressed femur growth. The low Ca plus phytate group showed the lowest Ca content of total femur and this was related to a significant decrease of Ca retention. Phytate intake depressed zinc(Zn) absorption in the first metabolic collection. This inhibitory effect of phytate on Zn absorption was improved in the low Ca plus phytate group after several weeks. Impared Zn absorption however remained in the high Ca plus phytate group which was reflected in the lowest Zn content of femur, phytate intake with high Ca also depressed phosphorous(P) absorption and serum and urinary P. These adverse effects of phytate on Zn and P absorption when the dietary Ca was high could explain reduced femur weight despite the highest concentration of femur Ca(mg/g ash) in this group. Results suggest that phytate can adversely affect not only Ca metabolism but Zn and P utilization. Thus, for the normal bone growth when phytate intake is high, the ingesion of Ca, P, Zn and other minerals should be enhanced.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.898-911
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• 2016

Sugars play many important roles in plant metabolism and directly influence fruit quality. The effects of two edible coatings, 2% calcium chloride and 1% pullulan, on sugar metabolism in postharvest Huangguan pear fruit were investigated during cold storage. The respiration rate, sugar content and composition, sucrose metabolism enzyme activities [acid invertase (AIV), neutral invertase (NI), sucrose synthase (SS), and sucrose phosphate synthase (SPS)] and expression of the AIV gene were analyzed during storage at $0^{\circ}C$ for 210 days. Coating treatments slowed the decrease of sucrose and hexose, the respiration rate, and the activities of AIV, NI, SS and SPS, thus maintaining high total soluble solids (TSS) and titratable acid (TA) contents in the fruit. There were no significant differences in AIV expression or activity between the treated and control groups of fruits. Both of the coatings could inhibit the activities of sucrose-cleaving enzymes, thus slowing the decrease of sugar content and maintaining high fruit quality during cold storage.