• 한국가축번식학회지
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• 제26권1호
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• pp.33-39
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• 2002

소 체세포 핵이식 시 융합액 내 $Ca^{2+}$ 농도에 따른 융합율, 핵형의 변화, 배 발달율을 검토한 결과는 다음과 같다. 1. 융합액 내 $Ca^{2+}$ 농도를 0.05, 0.1, 0.5 및 1.0 mM로 각기 다르게 처리한 결과, 0.5와 1.0 mM의 CaC$_2$에서 융합율이 80.5 와 84.3%로 나타나 0.05 mM CaCL$_2$에서의 융합율 56.6%에 비하여 유의적(P＜0.01)으로 높았다. 2. 융합액 내 $Ca^{2+}$ 농도에 따른 재구축배의 핵형을 검토한 결과, CaCL$_2$농도 0.05와 0.1 mM 에서는 88.4와 84.0%의 난자가 PCC이후 염색질괴를 형성한 반면, 0.5와 1.0 mM 에서는 54.5와 59.3%가 PCC 형태를 거치지 않고 직접 전핵을 형성하여, $Ca^{2+}$ 농도가 증가함에 따라 PCC 형태를 거치지 않고 직접 전핵을 형성하는 난자의 비율이 증가하였다. 3. 핵이식란의 체외 발육율을 검토한 결과, 1.0 mM CaCl$_2$에서는 배반포 발육율이 30.6%로 나타난 반면, 0.1 mM CaCl$_2$에서 는 20.0%로 나타나 유의적(P＜0.05)인 차이를 나타냈다. 본 연구의 결과는 융합액 내 $Ca^{2+}$농도의 증가가 소 체세포 핵이식란의 융합율 및 배반포 발육율을 향상시킬 수 있음을 시사한다.

• The Korean Journal of Physiology
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• 제28권2호
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• pp.143-150
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• 1994

The whole-cell mode of the patch clamp technique was used to study $Ca^{2+}-activated\;Cl^-\;current$ $(I_{Cl_{Ca}})$ in gastric antral myocytes. Extracellular application of caffeine evoked $Ca^{2+}-activated\;current$. In order to isolate the chloride current from background current, all known systems were blocked with specific blockers. The current-voltage relationship of caffeine-induced current showed outward rectification and it reversed at around $E_{Cl^-}$. The shift of reversal potential upon the alteration of external and internal chloride concentrations was well fitted with results which were calculated by the Nernst equation. Extracellular addition of N-phenylanthranilic acid and niflumic acid which are known anion channel blockers abolished the caffeine induced current. Intracellular application of a high concentration of EGTA also abolished this current. Application of c-AMP, c-GMP, heparin, or $AIF^-_4$ made no remarkable changes to this current. Sodium replacement with the impermeable cation N-methylglucamine or with $Cd^{2+}$ rarely affected this current. From the above results it is suggested that the caffeine induced current was a $Cl^-$ current and it was activated by intracellular $Ca^{2+}$.

• 한국포장학회:학술대회논문집
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• 한국포장학회 2006년도 정기총회 및 추계학술발표대회
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• pp.95-101
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• 2006

거창사과원예협동조합에서 구입한 사과 600상자를 5% $CaCl_2$ 용액에 각각 15분간 침지하는 방법으로 전처리 후 24시간 건조시키고 무포장구, $25{\mu}m$ 두께의 기능성 MA 필름으로 포장한 포장구와 무처리 무포장구인 대조구로 나누어 $0^{\circ}C$의 저온저장고로 옮겨 7개월 동안 저장하면서 3주 간격으로 품질을 분석하였다. 저장 기간이 증가할수록 중량 감소율은 Control 구보다 $CaCl_2$구와 $CaCl_2+MA$구의 중량 감소율이 월등히 낮았다. 경도변화는 저장 기간이 증가할수록 감소하는 경향을 보였으며, 그 중 $CaCl_2$구는 초기치 0.97 $kg_f$에서 저장 18주에는 0.95 $kg_f$로 초기치에 비해 2%의 가장 적은 변화를 보였다. 산도는 대조구보다 처리구의 산도가 높게 나타났으나 큰 차이는 나타나지 않았다. 가용성 고형분 함량은 저장 기간 모든 실험구에 따른 큰 차이가 없었다. 비타민 C 함량은 저장기간이 지날수록 모든 실험구에서 감소하는 경향을 보였으며, 기호도 조사에서 $CaCl_2$구와 $CaCl_2+MA$구가 대조구보다 유의적으로 높게 나타났다.

• KSBB Journal
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• 제9권3호
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• pp.294-298
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• 1994

$CaCl_2$, glucose, frutose, glutamine, glutamate 그리고 Ii pids와 같은 배지 첨가물들이 회분식 그리고 연속식 2단계 생물반응기 시스템에셔 재조합 ${\beta}$-galactosidase (${\beta}$-gal) 생산을 증진시키는가를 조사하였다. Sf 21 세포에 AcNPV의 감염시 $CaCl_2$, frutose, glutamate, cholesterol 및 tocoph eral과 같은 배지 첨가물을 첨가하였을 때 ${\beta}$-gal 생산이 증진되었다. 30mM $CaCl_2$, 2.2mM frutose, 4.lmM glutamine, 그리고 O.34mM cholesterol이 보강된 감염 배 지를 이용한 재조합${\beta}$-gal 생산은 약 40% 의 증가를 보였다.

• 동아시아식생활학회지
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• 제15권2호
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• pp.219-225
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• 2005

Effect of blanching and/or $CaCl_2$ addition on the quality characteristics of Oiji prepared by dry salting method, which has been used for industry, was investigated. Control$(15\%\;salt),\;CAO(15\%\;salt\;+\;0.04\%\;CaCl_2)$ and BCO $(15\%\;salt\;+\;0.04\%\;CaCl_2+blanching\;at\;60^{\circ}C\;for\;20\;min)$ for 165 days. Acidity was lower, but pH was higher in BCO than in the control or CAO. During the whole period of storage, greenness(-a) of BCO was maintained in Hunter color system, compared with the other groups. The numbers of total microbes, lactic acid bacteria and yeast were the lowest in BCO, while the highest in control. Moreover, texture profile analysis exhibited that fracturability and hardness maintained the highest in BCO during storage, compared with the other groups. Based on these results, combination of blanching and $CaCl_2$ addition is favorable to extend the shelf life and to maintain the good quality.

• 한국산학기술학회논문지
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• 제7권6호
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• pp.1292-1295
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• 2006

세 종류의 전해질 수용액(염화암모늄형, 염화아연형, 알칼리형)과 조해성재료($CaBr_2$ 또는 $CaCl_2$)를 filter paper 내부에 함침시켜 망간 일차전지용 전해질막을 제조하였다. 전해질막의 두께는 $250{\sim}300{\mu}m$ 이며 유연성 또한 매우 좋았다. 전해질막의 전기화학적 특성은 수분공급을 위해 첨가된 조해성 재료의 종류 및 함량에 크게 의존하였고, $CaCl_2$가 첨가된 계에서 상대적으로 높은 이온전도도 및 방전용량이 얻어졌다.

• Geomechanics and Engineering
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• 제25권3호
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• pp.245-251
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• 2021

The present study investigated the ageing effect on compressibility, permeability and shear strength behavior of kaolin and bentonite samples in the presence of NaCl and CaCl₂ solutions. The compressibility, permeability and shear strength parameters were determined on the 60, 190, and 250 days cured samples. The results have shown that, the kaolin sample becomes more compressible in the presence Ca²⁺ ions with ageing. Generally, the normalized compression index values of bentonite samples increased at the end of 60 days and 250 days curing time periods. The normalized permeability value of kaolin decreased by ageing in the presence of Na⁺ ions almost twofold. The permeability values of bentonite increased both in NaCl and CaCl₂ solutions during ageing. In the presence of Na⁺ ions kaolin had higher max. shear stress value than Ca²⁺ ions. When the max. shear stress values of 0, 60 and 190 days samples were compared, it was seen that NaCl solution had no significant effect on the shear strength of kaolin sample. However, the shear strength of kaolin increased in the CaCl₂ solution during ageing. In the presence of Ca²⁺ ions the max. shear stress value of bentonite was higher. The results of this study have shown that ageing has significant effects on the compressibility, permeability and shear strength of kaolinitic and bentonitic clayey soils.

• 인간식물환경학회지
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• 제23권4호
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• pp.445-453
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• 2020

Background and objective: Calcium chloride (CaCl₂) and sodium chloride (NaCl) are commonly used as a deicing agent in South Korea and penetrate the soil on the roadside, causing damage to plants. This study was conducted to investigate the salinity reduction effect of Pennisetum alopecuroides and the chemical characteristics of soil leachate. Methods: The plants were treated with five different concentrations of CaCl₂ (0, 1, 2, 5, and 10g·L^-1) and were grouped into the Cont., C1, C2, C5, and C10 groups. CaCl₂ of 200 m·L^-1 was sprayed to each plant once every two weeks. The growth of P. alopecuroides (plant height, leaf length, leaf width and the number of leaves) was measured. The level of EC and pH, and exchangeable cations (K⁺, Ca²⁺, Na⁺, and Mg²⁺) in the leachate of soil was monitored. Results: The pH of soil leachate decreased as the CaCl₂ concentration increased, and the EC increased significantly. The content of K⁺ did not change significantly until the concentration of CaCl₂ reached 5 g·L^-1, but the content of Ca²⁺, Na⁺, and Mg²⁺ significantly increased. The plant height, leaf length, and leaf width of P. alopecuroides showed the highest value in CaCl₂ 1 g·L^-1 followed by CaCl₂ 2 g·L^-1 and the control group. Root fresh weight was the highest in CaCl₂ 2 g·L^-1. On the other hand, there was no change in the shoot fresh weight, dry weight and root dry weight, and P. alopecuroides growth inhibition at the concentration of 5 g·L^-1 or higher in the plant height and leaf length. Conclusion: P. alopecuroides is relatively highly salt-tolerant and can improve the salt damaged soil by lowering the content of the salt-based exchangeable K⁺ ions.

• Clinical and Experimental Reproductive Medicine
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• 제28권1호
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• pp.13-24
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• 2001

Objective: In muscle and neuronal cells, calcium channels have been classified by electrophysiological and pharmacological properties into (1) voltage-dependent $Ca^{2+}$-channel (1) P/Q-type $Ca^{2+}$-channel (2) N-type $Ca^{2+}$-channel (3) L-type $Ca^{2+}$-channel (4) T-type $Ca^{2+}$-channel (5) R-type $Ca^{2+}$-channel. The present study was done in order to investigate whether there is any difference in $Ca^{2+}$-channel distribution between activated and normally fertilized embryos. Methods: The immunocytochemical method was used to identify the existence of voltage-dependent $Ca^{2+}$-channels in parthenogenetically activated 2-cell embryos by ethanol and $SrCl_2$ treatment. These 2-cell embryos were obtained by exposure to 6% ethanol for 6 min and to 10 mM $SrCl_2$ for 2h. Results: P/Q-type $Ca^{2+}$-channels and L-type $Ca^{2+}$-channels have been identified. Whereas, three type of $Ca^{2+}$-channel P/Q-type, N-type, L-type have been identified in 2-cell embryos fertilized in vivo. Conclusion: Activation by ethanol was faster than those by $SrCl_2$. However, there was difference in DAB staining of the embryos between ethanol and $SrCl_2$ treatment (87.7% and 54.1 %). Intensity of staining was also different between ethanol- and $SrCl_2$-treated group. However, it has not been known why there was some difference in DAB staining and staining intensity in the present study.

• 한국공간구조학회논문집
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• 제6권4호
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• pp.73-80
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• 2006

본 논문은 지하매설 철 구조물의 전기적 부식방지를 위해 Mg 희생양극을 사용하는 부식방지 기술에 대한 연구 또한 활발히 진행되고 있다. Mg 희생양극은 지하에 매설되는 철 구조물(파이프, 탱크, 파일, 고정 앵커 등)을 부식으로부터 보호하기 위하여 사용되는 것이다. 본 연구에서는 종래의 산화 소화용 표면 보호재로 이용되고 있는 비교적 값이 싼 CaCl2 염화물을 이용하여 마그네슘 합금제조 시 CaCl2 염화물의 표면보호 효과 및 제조된 Mg-Ca 합금들의 전기화학적 특성을 조사하였다 금속 Ca가 아닌 산화방지 및 소화 용제로 이용되고 있는 염화물(CaCl2)을 이용하여 자연부식 전위 값이 -1.695VSCE 이하, 사용효율도 59% 이상인 Mg-Mn-Ca 희생양극제의 제조기술을 확립하였다.