• Food Science and Preservation
• /
• v.30 no.3
• /
• pp.483-491
• /
• 2023

This study aimed to compare the bioactive compounds in Centella asiatica (C. asiatica) cultivated in a smart farm and a field and their effects on human keratinocyte cells. C. asiatica was collected in Jeju-do, Korea, and cultured in a smart farm and a field. The main bioactive compounds in the two differentially cultured C. asiatica were identified, and their activation in keratinocytes were assessed. Amplification and sequencing of the internal transcribed spacer (ITS) DNA in the nucleus and psbA-H DNA in the chloroplast were performed for species analysis. A comparison of DNA of plants reported in the NCBI GenBank was performed. The ITS DNA and psbA-H DNA sequences of C. asiatica cultivated in a smart farm and a field were consistent with No. MH768338.1 and No. JQ425422.1, respectively. Analysis of the triterpenes was performed using high performance liquid chromatography (HPLC) and as a result, C. asiatica cultured in a smart farm had more triterpenes than those cultured in a field. The effects of C. asiatica grown in a smart farm on cell proliferation and scratch recovery in HaCaT cells were greater than those grown in a field. These results suggest that C. asiatica cultivated in a smart farm can be effectively utilized as a health functional food.

• Journal of Environmental Health Sciences
• /
• v.24 no.1
• /
• pp.54-61
• /
• 1998

This study was conducted in order to evaluate the treatment efficiencies of anaerobic packed and fluidized-bed biofilm and to investigate applicability in treating livestock wastewater. Biocarbonate alkalinity(BA) and volatile fatty acid(VFA) were about 3,230-3,270 mg/l, 3,790-3,126 mg/l(as CaCO$_3$) and 224-402 mg/l, 141-387 mg/l(as CH$_3$COOH), and VFA/BA ratio was about 0.069~0.12, 0.045-0.12 in packed and fluidized-bed biofilm. When COD loading rate was 6.0 kg COD/$m^3\cdot$ day in packed and fluidized-bed biofilm, methane gas production were 3.23 l/day and 4.38 l/day, respectively. In the same COD loading rate, methane gas production volumes per kg COD removal were 0.25 m$^3$ CH$_4$/kg COD$_{rm}$ and $0.28 m^3 CH_4/kg COD^{rm}$, respectively. At this time, it could be estimated that fluidized-bed biofilm was more high. In case of HRT 0.94 day(6.0 kg COD/$m^3\cdot$ day) and 11 day(0.5 kg COD/$m^3\cdot$ day), packed-bed biofilm showed 59% and 81% COD removal efficiency, respectively. While fluidized-bed biofilm showed 72% and 85% removal efficiency, respectively. It was showed that fluidized-bed biofilm was more efficient. Packed-bed biofilm was higher than fluidized in treatment efficiencies of organic matters, but required continuous treatment using combined system, because it was very exceeded over an environmental standard solidified from '96 year. In operating fluidized-bed biofilm, if farm house consider high power cost according to high circulation ratio in an economic point of view, it would have an effect that farm house use packed-bed biofilm as combined system in treating livestock wastewater.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.40 no.1
• /
• pp.109-120
• /
• 2014

In this study, the extracts of Inula britannica var. chinensis (I. britannica) flower were extracted at three different temperatures (room temperature, $45^{\circ}C$, and $65^{\circ}C$) and their anti-aging effects were studied. Before investigating anti-aging effects of the extracts, their cytotoxicity was tested on B16F10, Hs683, and HaCaT cells. All extracts showed no cytotoxicity at the concentration less than 0.1% (v/v). Melanin synthesis inhibitory activities in B16F10 cells and reverse transcriptase polymerase chain reaction (RT-PCR) in Hs683 and HaCaT cells were used to see their anti-aging effects. The room temperature extract at 0.1% showed 24.5% melanin synthesis inhibition, which was better than the $45^{\circ}C$ and $65^{\circ}C$ extracts. In addition, expression rates of the room temperature extract at 0.1% on HAS-1, HAS-2, and HAS-3 related to hyaluronan synthase genes were 123.3%, 137.8%, 133.2%, respectively. which were higher than reference material of L-ascorbic acid. Expression rates of the $45^{\circ}C$ extract at 0.1% on TNF-${\alpha}$, COX-2, and IL-$1{\alpha}$, which are inflammatory related genes, was suppressed to 30.3%, 12.8%, 25.7%, respectively. It was better in anti-in flammatory effect than the room temperature and $65^{\circ}C$ extracts. As results, we showed that I. britannica var. chinensis flower extarcts decreased melanin production and expression of inflammatory related genes and increased the expression rate of hyaluronan synthase genes. Thus, it is believed that the extracts affect anti-aging effects of skin through whitening, moisturizing, and anti-inflammatory processes and could be applicable to cosmetics as a functional cosmetic ingredient.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.6
• /
• pp.744-751
• /
• 2011

Meat production and quality traits in beef cattle are largely affected by genetic factors. Acetyl-Coenzyme A carboxylase-${\alpha}$ (ACACA) plays a key role in the regulation and metabolism of fatty acid biosynthesis in mammalian animals. The gene encoding ACACA enzyme was chosen as a candidate gene for carcass and meat traits. In this study, we investigated effects of single nucleotide polymorphisms (SNPs) in the ACACA gene on beef carcass and meat traits in Hanwoo (Korean cattle) populations. We have sequenced a fragment of intron I region of the Hanwoo ACACA gene and identified two SNPs. Genotyping of the two SNP markers (g.2344T>C and g.2447C>A) was carried out using PCR-SSCP analysis in 309 Hanwoo steers to evaluate their association with carcass and meat production traits. The g.2344C SNP marker showed a significant increasing effect on LW (p = 0.009) and CW (p = 0.017). Animals with the CC genotype had higher CW and LW compared with TT and TC genotypes (p<0.05). The g.2447A SNP marker was associated with higher MC (p = 0.019). Animals with the AA genotype had higher MC than animals with CC and CA genotypes (p<0.05). Although the degree of linkage disequilibrium (LD) was not strong between g.2344T>C and g.2447C>A in the LD analysis, four major haplotype classes were formed with two SNP information within the ACACA gene. We constructed haplotypes using the HaploView software package program and analyzed association between haplotypes and carcass traits. The haplotype of ACACA gene significantly affected the LW (p = 0.027), CW (p = 0.041) and MC (p = 0.036). The effect of h1 haplotype on LW and CW was larger than that of h3 haplotype. Animals with the h1 haplotype also had greater MC than did animals with h2 haplotype. Consequently, the ACACA gene could be useful as a DNA marker for meat production traits such as carcass yield and meat contents in Hanwoo.

• Archives of Plastic Surgery
• /
• v.38 no.1
• /
• pp.1-6
• /
• 2011

Purpose: Currently, soft tissue filler products based on hyaluronic acid are widely used. They are safe, effective, and convenient to use compared with bovine collagens. However, all commercially available hyaluronic acid based fillers in Korea are imported ones. The purpose of this study is to evaluate efficacy of a new hyaluronic acid filler (HyaFilia; CHA bio&Diostech Co., Seoul), which has been recently developed in Korea. Methods: Three kinds of soft tissue fillers, including Restylane (Q-Med, Uppsala, Sweden), HyaFilia (CHA bio & Diostech Co., Seoul), Juvederm (Allergan, CA, USA), were injected subdermally into the back of hairless mice at six sites. The 2 cephalic sites composed Restylane groups, the 2 middle sites HyaFilia groups, and the 2 caudal sites Juvederm groups. Six hairless mice were included in the study, therefore, a total of 12 injections per group were performed. After 16 weeks after injection, the nodular swellings that resulted from the injections were excised wide enough to include skin beyond the swelling points down to the panniculus carnosus layer using 8mm punches. Volumes and weights were measured using a stereoimage optical topometer system and a weighting machine. Histologic comparisons were also carried out. Results: The mean volumes of the Restylane, HyaFilia and Juvederm groups were $3698{\times}103$, $4820{\times}103$, and $1435{\times}103$ PI, respectively. The mean weights of the Restylane, HyaFilia and Juvederm groups were 36.08, 37.83, and 24.66 mg, respectively. Histologic examination between the 3 groups showed no significant difference in tissue compositions and inflammatory reactions. Conclusion: The results of this study shows that HyaFilia is superior to Restylane and Juvederm in longavity of filling effect. Therefore, HyaFilia may be an effective replacement for the imported materials including Restylane and Juvederm.

• Korean Journal of Food Science and Technology
• /
• v.14 no.4
• /
• pp.388-393
• /
• 1982

Lipids extracted from two garlic samples (Allium sativum Linnaeus) were studied. Total lipid content of the samples ranged from 310 to 342mg/100g of fresh garlic. These lipids were shown to consist of $36.4{\sim}43.5%$ neutral lipids, $20.3{\sim}24.3%$ glycolipids, and $36.2{\sim}39.3%$ phospholipids. Among the neutral lipids, triglycerides were predominant $(80.5{\sim}83.6%)$ with smaller amounts of free sterols, free fatty acids, and sterol esters being present. 1, 2-Diacylglycerol acetates, 1, 3-diacylglycerol acetates and cholesterol acetates were also tentatively identified. Esterified steryl glycosides and steryl glycosides were major sugar-containing lipids, but monogalactosyl diglycerides, cerebrosides, digalactosyl diglycerides and sulfolipids were relatively minor components. Of the phospholipids, phosphatidyl cholines and serines, and phosphatidyl ethanolamines were the major components, comprising over 85% of this class. Phosphatidic acid and phosphatidyl inositols were also present. The major fatty acids in the total and three lipid classes were linoleic, palmitic, oleic and linolenic acid.

• Clean Technology
• /
• v.24 no.3
• /
• pp.212-220
• /
• 2018

Oxy-fuel combustion is considered as a promising greenhouse gas reduction technology in power plant. In this study, the behaviors of NO and $SO_2$ were investigated under the condition that in-furnace $deNO_x$ and $deSO_x$ methods are applied in oxy-fuel circulating fluidized bed combustion condition. In addition, the generation trends of $SO_3$, $NH_3$ and $N_2O$ were observed. For the purpose, limestone and urea solution were directly injected into the circulating fluidized bed combustor. The in-furnace $deSO_x$ method using limestone could reduce the $SO_2$ concentration in exhaust gas from ~403 to ~41 ppm. At the same experimental condition, the $SO_3$ concentration in exhaust gas was also reduced from ~3.9 to ~1.4 ppm. This trend is mainly due to the reduction of $SO_2$. The $SO_2$ is the main source of the formation of $SO_3$. The negative effect of $CaCO_3$ in limestone, however, was also appeared that it promotes the NO generation. The NO concentration in exhaust gas reduced to ~26 - 34 ppm by appling selective non-catalytic reduction method using urea solution. The $NH_3$ concentration in exhaust gas was appeared up to ~1.8 ppm during injection of urea solution. At the same time, the $N_2O$ generation also increased with increase of urea solution injection. It seems that the HNCO generated from pyrolysis of urea converted into $N_2O$ in combustion atmosphere. From the results in this study, the generation of other pollutants should be checked as the in-furnace $deNO_x$ and $deSO_x$ methods are applied.

• Clean Technology
• /
• v.29 no.2
• /
• pp.95-101
• /
• 2023

An experiment was conducted to produce vaterite-type precipitated calcium carbonate from waste oyster shells in order to use them as recyclable resources. Calcined oyster shells containing calcium oxide as their main component were prepared at a temperature of 800℃ for 24 h. The oyster shells were dissolved in nitric acid or hydrochloric acid solution to make 0.1 M calcium nitrate or calcium chloride aqueous solution, and a carbonation reaction was performed using a 0.1 M sodium carbonate aqueous solution under various experimental conditions, which included varying the amount of aspatic acid additive, the amount of NH₄OH added, the reaction time, the reaction temperature, the stirring speed, and the type of dissolved acid. The XRD, SEM, and size distributions were analyzed and the vaterite content was calculated. Spherical precipitated calcium carbonate with a vaterite content of 95.9% was synthesized by adding 0.1 mol aspatic acid/1 mol CaO and 2 cm³ of NH₄OH, and reacting for 1 h at 25℃ while stirring at 600 rpm. The average particle diameter was found to be 12.11 ㎛. Calcium carbonate contatining high vaterite is used as high value added calcium carbonate for medical, food, inke additiver, etc.

• Journal of Nutrition and Health
• /
• v.34 no.1
• /
• pp.48-53
• /
• 2001

As the severity of environmental pollution increases, the foodstuffs are contaminated more the ever. There are 40 kinds of heavy metals that we are able to consume through the heavy metal contaminated-foodstuffs. Arsenic, lead, cadmium, and chromium out or them have been know to be a potential cause for a alzheimers disease, kidney diseases, and cancer. However, research data on the daily intakes of such heavy metals are limited. This study was performed to evaluate the daily intakes of nutrients and the dietary heavy metals of the college women living in Choongchung-NamDo Area, where had been reported to have high contents of heavy metals for the seafoods. We also investigated the blood levels of the heavy metals. The mean age, height, weight, BMI and percent ideal body weight(PIBW) of the subjects were 20$\pm$1.0yr, 158.4$\pm$0.7cm, 55.1$\pm$1.4kg, 22.4$\pm$.04, and 103.3$\pm$2.5 %, resp-ectivly. The mean of daily energy intake was 1,717.03$\pm$55.99kacl/day(86% of RDA for women). The ratio to energy from carbohydrate, fat and protein was 60: 24: 16. Daily intakes of Vit A, Vit B$_2$, CA, and Fe were under the RDA for those nutrients. The mean adequate ratio of the subjects was 0.92 . The daily intakes of heavy metal, such as As, Pb, Co, Cr and Mn , were 1.80$\pm$0.27mg, 75.21$\pm$4.12$\mu\textrm{g}$, 21.12$\pm$12.34$\mu\textrm{g}$, 60.07$\pm$6.24$\mu\textrm{g}$ and 6.23 $\pm$0.12mg respectively. the blood levels of As, Pb, Co, Cr and Mn were 16.10$\pm$2.10$\mu\textrm{g}$/dl, 4.32$\pm$0.58$\mu\textrm{g}$/dl, 0.02$\pm$0.01$\mu\textrm{g}$/dl, 4.23$\pm$0.41$\mu\textrm{g}$/dl, and 4.40$\pm$0.21$\mu\textrm{g}$/dl, respectively. In conclusion, daily intake of heavy metals for the college women living in Choongchung-NamDo area was lower than that of WHOs re-commendation, however, the blood levels of each heavy metals were higher than those of Japanese, american, and italian, There were no correlations between the dietary intakes and blood levels of each heavy metals. (Korean J Nutrition 34(1):48-53, 2001)

• Restorative Dentistry and Endodontics
• /
• v.33 no.6
• /
• pp.499-506
• /
• 2008

The purpose of this study was to compare the push-out strength of a fiber post cemented with various resin cements. Newly extracted 36 human mandibular premolars which had single root canal were selected and their crown portions were removed. The root canal was instrumented using $PROTAPER^{TM}$ system and obturated using continuous wave technique. In each root, a 9-mm deep post space was prepared. #2 translucent fiber post (DT Light post, Bisco Inc., Schaumburg, IL, U.S.A.) was cemented using injection technique with Uni-dose needle tip (Bisco) and six different resin cements. The tested resin cements were Duo-Link (Bisco Inc., Schaumburg, IL, U.S.A.), Variolink II (Ivoclar-Vivadent AG, Schann, Liechtenstein), Panavia F (Kuraray Medical Inc., Okayama, Japan), Multilink Automix (Ivoclar-Vivadent AG, Schann, Liechtenstein), RelyX Unicem (3M ESPE Dental Products, St. Paul, MN, U.S.A.), and Maxcem (Kerr Co., CA, U.S.A.). After storage in distilled water for 24 hours, each root was transversally sectioned into approximately 1-mm thick sections. This procedure resulted in 6 serial sections per root. Push-out test wasperformed using a universal testing machine (EZ Test, Shimadzu Co.) with a crosshead speed of 1 mm/min. The data were analyzed with one-way ANOVA and Tukey HSD (p=0.05). The push-out strength of the groups which cemented fiber post with Panavia F and Multilink Automix were lower than those of the other groups. But, there were no statistically significant difference among groups at a probability level of 0.05.