• Microbiology and Biotechnology Letters
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• v.1 no.2
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• pp.75-78
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• 1973

Effects of nitrogen sources, supplement of vitamins and minerals on the accumulation of 5'-inosinic acid by an adenineless mutant of Brevibacterium ammoniagenes were examined. Maximal yield of 5'-inosinic acid was attained by the use of yeast extract. as organic nitrogen source Casamino acid supplemented with $Mn^{＋＋}$, $Zn^{＋＋}$ Ca-D-pantothenate and thiamine HCl could be subsitute for it. Subsequent experiment using the defined medium showed that the concentration of these trace element in the medium affected inosinic acid accumulation markedly. And it was found that the simulataneous addition of $Mn^{＋＋}$ (20$\mu\textrm{g}$1). $Zn^{＋＋}$(10$\mu\textrm{g}$1). thiamine Hcl(10mg/1) and Ca-D-pantothenate (5mg/l) to the defined medium stimulated inosinic acid accumulation, from which 7mg/ml of inosinic acid was obtained.

• Korean Journal of Poultry Science
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• v.23 no.1
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• pp.27-37
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• 1996

This experiment was carried out to study the effects of feeding split diets for a.m. and p.m. on laying hen performance and feed cost with 480 ISA Brown layers for three periods during 32∼36, 52∼56 and 72∼76 wk of age, respectively. The control group(C) was fed a conventional single diet throughout the day and split diet groups (T$_1$, T$_2$, T$_3$, T$_4$ and T$\_$5/) were offered high energy-high protein-low Ca diets, and low energy-low protein-high Ca diets in a.m. and p.m., respectively. In split diet groups, feed intake, ME and CP consumption, and feed cost were significantly reduced(P<0.05) compared to the C, while the hen-day egg production was significantly improved. However, the average egg weight was not influenced by the feeding system. Due to the reduced daily feed, ME, and CP intakes, and the improved egg production, the conversions of feed, ME, CP, and feed cost required per kg egg mass were also significantly improved (P<0.05) in splits diet groups. Among split diet groups, the T$_2$ group fed the diet devoid of Ca supplement from the conventional laying hen diet in a.m., and the diet containing the Ca supplement 2 times the conventional diet in p.m., was superior in conversion of nutrients and feed cost per kg egg mass to the other split diet groups. It was concluded that daily feed and nutrients cousumption and daily feed cost could be reduced 5∼6％ while hen-day egg production could be improved about 4％, so that about 10％ of the feed and nutrients and feed cost per kg egg mass could be spared by introducing the split diet feeding for a.m. and p.m. based on T$_2$ treatment in laying hens.

• Journal of Environmental Science International
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• v.23 no.5
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• pp.787-792
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• 2014

The objective of this study was to investigate the effects of the Crataegus pinnatifida BUNGE extract supplementation of non esterified fatty acid (NEFA), concentrations of serum protein and electrolyte in sera on the hyperlipidemic rats. Concentrations of NEFA and globulin were remarkably lower in the Crataegus pinnatifida BUNGE extract group (HW group) than in the hyperlipidemic group (HD group), but no difference between control group (CO group) and extract of Crataegus pinnatifida BUNGE supplement in control group (NW group). However, concentrations of electrolyte K and A/G were higher in the HW group than HD group. Concentrations of total protein, albumin, electrolyte of total Ca, Pi, Na and Cl were no difference between HW group than HD group. The results indicate that Crataegus pinnatifida BUNGE extract was in the improvement of hyperlipidemic rats.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.217.1-217.1
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• 2003

Phytoestrogen has been used as supplement in order to treat osteoporosis. The representative phytoestrogen, isoflavones, are daidzein, genistein and formononetin which were present highly in legumes. We have studied the quantitative analysis of isoflavones in KUNBO by HPLC. KUNBO, a mixed herbal extract including Astragali Radix and Rhynchosiae nulubilis Semen (Leguminosae), etc., is a nutraceutical candidate for type I osteoporosis. olumn used in HPLC was LUNA 5${\mu}$ C18 (250 ${\times}$4.6mm) (Phenmenex Co.m Torroance, CA, U.S.A.). (omitted)

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.1
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• pp.26-33
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• 2002

In order to assess the effect of feeding raw or processed cotton (Gossypium) seed meal (CSM) on meat and wool production, 30 male crossbred lambs (3-4 months) of uniform body weight were assigned equally to five dietary treatments in a completely randomised design. The CSM was processed by three different methods i.e., cooking the meal at $100^{\circ}C$ for 45 minutes, treatment with 1% calcium hydroxide ($Ca(OH)_2$) for 24 h and iron treatment in the ratio of 1 part free gossypol (FG) to 0.3 parts of iron for 30 minutes. The lambs were fed isonitrogenous and isocaloric concentrate mixtures, containing 30% deoiled peanut meal (reference diet) and 40% of either raw, cooked, $Ca(OH)_2$ or iron treated CSM for 180 days. The raw and variously processed CSM replaced about 50% nitrogen of reference concentrate mixture. The concentrate mixtures were fed to meet 80% of the protein requirements (NRC, 1985) along with ad libitum chopped maize (Zea mays) hay. The slaughter weight, empty body weight and carcass weight was higher ($p{\leq}0.01$) in lambs fed cooked CSM incorporated diets, compared to diets containing deoiled peanut meal (DPNM). These parameters were not influenced by feeding diets containing either raw, $Ca(OH)_2$ or iron treated CSM in comparison DPNM diets. The carcass length, loin eye area and edible and inedible portion of carcass and the meat: bone ratio in whole carcass were also not affected by feeding CSM based diets. Among various primal cuts, the yield of legs was lower ($p{\leq}0.05$) from raw CSM fed lambs in comparison to DPNM fed lambs. The fat content in the Longissimus dorsi muscle was reduced ($p{\leq}0.05$) in lambs fed processed CSM based diets compared to those fed DPNM diet. Replacing DPNM with either raw or processed CSM based diets did not influence the sensory attributes and overall acceptability of meat. The wool yield was higher ($p{\leq}0.05$) in iron treated CSM fed lambs. The fibre length and fibre diameter were comparable among lambs on various dietary regimes. Among lambs fed variously processed CSM diets, the feed cost per kg of edible meat production was lower ($p{\leq}0.05$) on $Ca(OH)_2$ treated CSM, followed by cooked CSM diet and then on raw CSM based diets compared to DPNM diet. The CSM after 1% $Ca(OH)_2$ treatment or cooking for 45 minutes appears to be a satisfactory protein supplement in lamb diets for meat and wool production to replace at least 50% nitrogen of scarce and costly peanut meal.

• Korean Journal of Community Nutrition
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• v.4 no.2
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• pp.207-218
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• 1999

The increased number of elderly in Korea presents great challenges for community nutrition programs. Strategies to enhance elderly nutrition are needed, as well as dietary advice that are reality-based and tailored to the need of the elderly. The purpose of this study was to evaluate the overall effectiveness of an elderly nutrition improvement program(ENIP), by assessing changes in nutrition knowledge, attitudes, and behavior after program completion. ENIP was conducted in Suwon for 4 months in 1998 at 5 centers for the elderly. The program provided individual nutrition counseling and brief biweekly group education sessions. To stimulate participation and motivation, the elderly in two centers were served free milk packs(200ml) three time a week during the program(milk＋education group), and the elderly in 3 other centers were served yoghurt on the program day(education group). Altogether, 102 subjects finished the program(milk＋education group 67 ; education group 35). Data about nutrition knowledge, dietary attitudes, diet records(24 hour recall) and dietary habit was collected before(baseline) and after the program(follow-up). The Drop-out rate for the milk＋education group(2.9%) was significantly lower than that for the education group(30.0%)(p<.001). The Nutrition Knowledge fo females milk＋education group increased significantly after the program, but no significant differences were observed in other groups. Over 60% of all the elderly showed positive responses to the healthy eating attitudes. Energy, protein, vitamin A, riboflavin, Calcium and Potassium intakes improved in the male milk＋education group. The Vitamin C intake improved in the male education group. Energy and vitamin A intakes improved in the female milk＋education group and energy, vitamin A, thiamin, riboflavin, niacin, Ca nad P in the female education group. A stepwise multiple regression analysis was performed to examine the effects of food intake changes on overall nutrient quality. For all elderly, the fish intake explained 12.6% of the variance, followed by candy intake and vegetable intake(model $R^2$=19.6%). Dietary habits such as meal time regularity and salty food reduction were markedly improved in both groups. Sixty-one percent of the milk＋education subjects stated their participation was voluntary and active, while 51% of education subjects did. Sixty four percent of the subjects stated their interest on diet increased remarkably through program participation. Considering the results, it is conjectured that ENIP had a great impact on nutrient intake, dietary attitudes and habits. Milk supplement showed no direct effects on elderly nutrition improvement, but it encouraged the program by reducing drop-out rates and inducing voluntary participation. Therefore milk supplement could enhance the community nutrition education program. Moreover it is strongly suggested that the ENIP be expanded to other communities.

• Journal of Nutrition and Health
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• v.37 no.9
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• pp.809-816
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• 2004

The purpose of this study was to evaluate the effect of maternal nutritional status and health behaviors on the concentrations of minerals (Zn, Fe, Ca) and the immunological substances (lactoferrin, sIgA, Iysozyme) in breast milk. Breast milk was collected from 193 healthy Korean women from obstetric clinics and postpartum care centers in Seoul. : 99 colostrum (1 - 5 days postpartum), 33 transitional milk (6 - 10 days postpartum), 61 mature milk (11 - 50 days postpartum). The concentrations of minerals and immunological substance were highest in colostrum and decreased with lactational period. Concentrations of Zn and Fe reduced significantly from colostrum to mature milk, however, Ca concentration stayed constant throughout the lactational period. Contents of lactoferrin, sIgA, and lysozyme were significantly lower in mature milk than in colostrum. Mother's nutritional status, assessed by prepregnancy BMI, had an effect only on colostrum, but not on transition and mature milk. Fe concentration of colostrum was significantly lower in underweight (prepregnancy BMI < 18.5) than in overweight mothers (prepregnancy BMI $\geq$ 23.0). Also lower tendency was observed for sIgA and lysozyme contents, even though the difference was not statistically significant. Pregnancy weight gain had no effect on the breast milk component. Since nutritional factors had some effect on colostrum, the health behaviors of mothers providing colostrum were assessed. The mother's behavior of smoking, drinking, morning sickness, parity, disease, nutrient supplement use had no significant effect on the breast milk component, however, Zn, sIgA, and lysozyme were the somewhat affected components by maternal health behavior.

• Journal of Animal Science and Technology
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• v.58 no.12
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• pp.42.1-42.8
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• 2016

Background: Deficiency of macro and micro-minerals in the ration of dairy cows adversely affects growth, milk production and reproduction efficiency. It is essential to examine mineral concentrations in feeds offered to dairy cows in practical farms. Methods: Two villages from each taluka were selected at random for taking representative samples of feeds, forages and hair. Within the village, help was sought from village milk producers and district animal husbandry officer for identification of 4 to 5 farmers and collection of representative samples. All the samples were processed and analyzed for chemical composition as well as major macro and micro-minerals, using Inductively Coupled Plasma-Optical Emission Spectrometer. Results: Ca content in wheat straw (0.29%), crushed maize (0.02%) and wheat bran (0.12%) was found to be below the critical level (0.30%). The P content in concentrate ingredients was high (0.26-0.96%), but low in dry roughages (0.06-0.12%). Cereal straws (0.14%) and grains (0.12%) were deficient in Mg. Feeds and forages were found to be adequate in K (1.50%). Cereals straws were found to be deficient in S (0.11%). Greens were good source of Cu (12.02 ppm). Wheat straw was found to be low in Zn (18 ppm), but high in Mn (225 ppm) and Fe (509 ppm). Local grasses and azolla green were found to be rich source of Co (>1.00 ppm). Se (0.63 ppm) was present in appreciable quantities in most of the feedstuffs. Conclusions: From the present study, it was apparent that the feeds and forages available in the state of Jharkhand may not meet the requirements for Ca, P, Mg, Cu, Zn and Co in order to sustain a milk production of ~10 kg/day. Therefore, it is necessary to supplement these deficient minerals through area specific mineral mixture in the ration of dairy cows for improving productivity and reproduction efficiency.

• Journal of Nutrition and Health
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• v.37 no.6
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• pp.423-430
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• 2004

This study was performed to evaluate the effect of red-yeast-rice on bone metabolism in overiectomized (OVX) rats. Forty female Sprague-Dawley rats (body weight 210 $\pm$ 5 g, 9 weeks old age) were divided into two groups. One group were OVX, and the other group received sham operation (SHAM), and received either control diet (20％ casein) or a red-yeast-rice power supplemented diet (0.1％) for 9 weeks. And then each rat group was further divided into control diet (casein 20％) and red-yeast-rice powder supplemented (0.1％) diet group. All rats were fed on experimental diet and deionized water ad libitum for 9 weeks. Bone mineral density (BMD) and bone mineral content (BMC) were measured using PIXImus (GE Lunar Co, Wisconsin, USA) in spine and femur on 5, 9 weeks after feeding. The serum and urine concentrations of Ca and P were determined. Bone formation was measured by serum osteocalcin and alkaline phosphatase (ALP) concentrations. And bone resorption rate was measured by deoxypyridinoline (DPD) crosslinks immunoassay and corrected for creatinine. Serum osteocalcin, growth hormone, IGF-l and calcitonin were analyzed using radioimmunoassay kits. Urinary Ca and P excretion were not significantly different among the groups. Within the OVX group, the red-yeast-rice group had a lower crosslinks value than the casein group. Therefore the red-yeast-rice supplemented groups had a lower bone resorption ratio than the casein group in the ovariectomized rats. And, the red-yeast-rice group had significantly higher IGF-l hormone than casein group in ovariectomized rats. The red-yeast-rice group had higher spine bone mineral content than those of control group within the OVX groups. This study was an important first step in establishing that the observed beneficial effects of red-yeast-rice on bone, and this study also established the need for a study on the long-term effect of this supplement in a human.

• Journal of Life Science
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• v.17 no.11
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• pp.1539-1546
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• 2007

We have investigated whether the supplement of magnesium to cold blood cardioplegia improves myocardial protection. Sixty patients scheduled for elective valvular heart surgery were randomly assigned to a control group (n=30) which received conventional cold blood cardioplegia and an Mg group (n=30) which received cold blood cardioplegia supplemented with 2 g of magnesium sulfate. Electrolytes levels including $Mg^{++}$, hematological and biochemical variables, cytokines, myocardial marker levels, and postoperative outcomes were compared between two groups before, during or idler operation. $Mg^{++}\;and\;Ca^{++}$ levels in the Mg group were higher than those of the control group after surgery. The total WBC counts, CK-MB, troponin-I and Interleukin-6 levels in the Mg group were lower than those of the control group after surgery. Postoperative incidence of atrial fibrillation was lower in the Mg group compared with the control group. These results showed that $Mg^{++}$ attenuated inflammatory reaction, myocardial damage, and hypomagnesemia during valvular surgery and reduced postoperative arrhythmia incidence without side effects.