• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1094-1104
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• 2018

The peel of astringent persimmon (Diospyros kaki Thunb. cv. Cheongdo-Bansi) is a by-product of dried persimmon (gotgam). We investigated if deastringent peel extracts of persimmon cv. Cheongdo-Bansi had antioxidative and neuroprotective properties. Two different extracts were prepared: thermally and nonthermally treated persimmon peel extracts (TPE and NTPE, respectively). Both TPE and NTPE were fractionated sequentially in n-hexane, chloroform, ethyl acetate, n-butanol, and water. The TPE and NTPE ethyl acetate fractions had the highest total phenolic and flavonoid contents as well as antioxidant capacities among all the fractions. Pretreatment of neuronal PC-12 and SH-SY5Y cells with the TPE and NTPE ethyl acetate fractions increased cell viability after exposure to oxidative stress. The ethyl acetate fraction of TPE attenuated oxidative stress inside both PC-12 and SH-SY5Y cells more effectively than that of NTPE. Furthermore, the TPE and NTPE ethyl acetate fractions inhibited acetylcholinesterase and butyrylcholinesterase. Analysis of ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry results revealed gallic acid, kaempferol, kaempferol-3-O-galactoside, kaempferol-3-O-glucoside, quercetin, quercetin3-O-galactoside, quercetin-3-O-galactoside-2'-O-gallate, and quercetin-3-O-glucoside as the major phenolics of the TPE and NTPE ethyl acetate fractions. Taken together, these results suggest that the ethyl acetate fraction of deastringent persimmon peel is rich in antioxidants and has potential as a functional food to reduce oxidative stress.

• 대한한방내과학회지
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• 제19권2호
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• pp.37-49
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• 1998

To make a objective diagnosis of the syndrome of cold-limbs, We investigated the 20 patients with cold limbs and GI trouble and 20 normal people as a control group. And we compared the thermal difference between Chon-jung(CV17) and Chung-wan(CV12 中脘), Chon-jung(CV17) and Ki-hae(氣海 CV6) and we compared the thermal differences of No-gung(PE8 勞宮) and Yong-chon(湧泉 KI1), too. The results were as follows. 1. All 20 patients had GI trouble and cold limbs. They had the symptom-Indigestion(16 cases-80%) with heart burn, tympanites, abdominal distention, hiccup, belching. Beside that symptom they also had constipation(6 cases-30%), diarrhea(3 cases-15%), headache & dizziness(6 cases-30%). And some had the menstrual syndrome, chronic fatigue, palpitation, insomnia, edema, arthralgia. 2. The thermal difference of the palms between the patients group and the control group were $25.70^{\circ}C,\;25.82^{\circ}C$, but they were not significant. 3. The thermal difference of the soles between the patients group and the control group were $23.58^{\circ}C,\;24.42^{\circ}C$ and the significancy was P=0.020 so it was significant(P<0.05). 4. The thermal difference of the palms and Chon-jung(CV17) between the patients group and the control group were $1.08^{\circ}C,\;0.76^{\circ}C$, but they were not significant. 5. The thermal difference of the sales and Chon-jung(CV17) between the patients group and the control group were $3.01^{\circ}C,\;1.90^{\circ}C$ and the significancy was P=0.003 so it was significant(P<0.05). 6. The thermal difference of Chon-jung(CV17) and Chung-wan(CV12 中脘) between the patients group and the control group was $0.30^{\circ}C,\;0.62^{\circ}C$ and the significancy was P=0.793 so it was significant(P<0.05). 7. The thermal difference of Chon-jung(CV17) and Ki-hae(CV6 氣海) between the patients group and the control group was $0.53^{\circ}C,\;0.68^{\circ}C$, but they were not significant. From the above, I could find the significance of D.I.T.I to diagnose the cold-feet not the cold-hands and the thermal difference between the trunk and limbs was more significant on cold-feet than on cold-hand, either.

• 대한한방내과학회지
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• 제35권1호
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• pp.24-36
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• 2014

Objectives : This study was performed to evaluate the effects of moxibustion on cerebral blood flow, peripheral blood flow and blood pressure. Methods : We designed a randomized, cross-over study that included 20 healthy, male volunteers who were randomly assigned to one of two groups (the moxibustion group or the control group). The initial trial was followed by a washout period of one week before the groups were switched and the trial repeated. The cerebral blood flow and peripheral blood flow of the volunteers in both groups were measured by mean velocity (Vm), peak systolic velocity (Vs), pulsatility index (PI), perfusion units (PU) and mean blood pressure (BP) before the intervention (baseline), 30 minutes after the intervention, and again 60 minutes after the intervention. Results : 1. The Vm increased significantly in the moxibustion group. The Vs also constantly increased in moxibustion group, but there was no significant difference. 2. The PI decreased significantly in the control group at 0-30 minutes and in the moxibustion group at 30-60 minutes. 3. The PU constantly decreased in moxibustion group. In control group, the PU decreased at 30 minutes and increased slightly at 60 minutes. 4. The mean blood pressure did not show any significant difference between the two groups. Conclusions : This study shows that moxibustion at CV12 increased the cerebral blood flow. Based on this result, we can suggest that moxibustion at CV12 might be helpful in improving the functional recovery of stroke patients. However, further research needs to be conducted on a larger numbers of subjects in order to confirm these effects.

• Journal of Acupuncture Research
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• 제21권6호
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• pp.63-84
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• 2004

Objective & Methods : To study the effects of anti-cancer, anti-metastasis and immune response improvement of herbal-acupuncture with Eclipta prostrata diffusae herba infusion solution(ELP-HAS), we injected ELP-HAS into Chung-wan(CV12) of C57BL/6 mice implanted intravenously with B16-F10 melanoma. We have reached the following conclusion through the effect on the percentage of $CD25^+/CD4^+$, $CD8^+/CD3e^+$, $CD69^+/B220^+$, $NK1.1^+/CD3e^+$cells in mouse PBMCs, the effect on the pulmonary colony formation number, and the effect on MST(Median Survival Time) and ILS(Increase of Life Span) of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results : The results were obtained as follows : 1. In the experiment groups treated with ELP(Ecliptae Herba) Herbal acupuncture, the spleen cell proliferation in BALB/c mouse was significantly increased compared with control group. 2. In the experiment groups treated with ELP(Ecliptae Herba) Herbal acupuncture, the percentage of $CD25^+/CD4^+$, $CD8^+/CD3e^+$, $CD69^+/B220^+$, $NK1.1^+/CD3e^+$cells in C57BL/6 mouse PBMCs was increased compared with control group. 3. In the experiment groups treated with ELP(Ecliptae Herba) Herbal acupuncture, the pulmonary colony formation number of C57BL/6 mice implanted intravenously with B16-F10 melanoma was decreased significantly compared with control group. 4. In the experiment groups treated with ELP(Ecliptae Herba) Herbal acupuncture, MST(Median Survival Time) and ILS(Increase of Life Span) of C57BL/6 mice implanted intravenously with B16-F10 melanoma were increased significantly compared with control group. Conclusions : ELP Herbal acupuncture injection into Chung-wan(CV12) of C57BL/6 mice is considered to be effective in anti-cancer, anti-metastasis and immune response improvement.

• Korean journal of health promotion
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• 제17권2호
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• pp.109-118
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• 2017

연구배경: 아동기 인구 집단에서 허리둘레/신장 비율을 기준으로 한 복부비만이 체질량지수를 기준으로 한 전체비만보다 심혈관건강과 더 강력한 부적관련성을 보고하고 있으나, 아직 그 근거가 부족하다. 이에 본 연구는 아동기 인구 집단에서 체질량지수는 물론이고 '허리둘레/신장 비율'과 심혈관건강 간의 관련성을 규명하고자 한다. 방법: 본 연구는 제5기 국민건강영양조사 원시자료(2010-2012년)를 활용한 횡단적 역학연구설계에 의해, 10-18세인 2,363명을 대상으로 성별(소년/소녀 집단)과 연령별 집단(10-12세/13-18세)으로 구분하여 분석하였다. 전체비만은 체질량지수 백분위수에 의해 비과체중군(<85), 과체중군(${\geq}85$)과 비만군(${\geq}95$)으로, '허리둘레/신장 비율'을 기초로 정상군(<0.5)과 복부비만군(${\geq}0.5$)으로 구분하였다. 심혈관건강은 7개 하부지표와 통합지표인 심혈관건강점수(표준화점수, z-score)로 분석하였다. 아동의 인구사회학적 특성과 부모의 사회경제적 특성을 보정한 후 복합표본 일반선형회귀분석을 수행하였다. 결과: 체질량지수를 기준으로, 비만군은 비과체중군에 비해 7개 심혈관개별지표와 심혈관건강점수 모두에서 통계적으로 유의하게 심혈관건강이 악화되어 있었다(P<0.05). '허리둘레/신장 비율'을 기준으로, 그 관련성 또한 위와 유사한 패턴을 보였으나(P<0.05), 이는 체질량지수 비만도를 보정한 후에도 여전히 13-18세 인구 집단에서는 심혈관건강점수가 비만군에서 비과체중군에 비해 통계적으로 유의하게 악화되어 있었으며, 이 패턴은 특히 남학생에서 유의하게 나타났다(P<0.01). 결론: 국내 13-18세의 소년 집단에서 '허리둘레/신장 비율' 복부비만 지표는 체질량지수를 보정한 후에도 심혈관 건강과 밀접한 관련성이 있었다. 이에 늦은 청소년 집단에서 '허리둘레/신장 비율'은 체질량지수보다 강력한 심혈관 건강을 반영할 수 있으므로, 학령기 청소년 집단을 위한 심혈관질환 조기 예방과 건강증진 차원에서 복부비만 지표는 간호사정의 지표로 고려될 필요가 있겠다.

• 대한약침학회지
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• 제15권1호
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• pp.12-17
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• 2012

Objectives: This study was designed to investigate the protective effects of Atractylodis Rhizoma pharmacopuncture (ARP) against acute gastric mucosal lesions induced by compound 48/80 in rats. Methods: The ARP was injected in Joksamni (ST36) and Jungwan (CV12) 1 hr before treatment with compound 48/80. The animals were sacrificed under anesthesia 3 hrs after treatment with compound 48/80. The stomachs were removed, and the amounts of gastric adherent mucus, gastric mucosal hexosamine, thiobarbituric acid reactive substances (TBARS), xanthine oxidase (XO), and superoxide dismutase (SOD) were measured. Also, histological examination were performed. Results: Gastric adherent mucus, gastric mucosal hexosamine and histological defects of gastric mucosa declined significantly after ARP treatment. Changes in gastric mucosal TBARS were also reduced by ARP treatment, but this result was not statistically significant. ARP treatment did not change the XO and the SOD activities. Conclusions: ARP showed protective effects for acute gastric mucosal lesions induced by compound 48/80 in rats. These results suggest that ARP may have protective effects for gastritis.

• 농업과학연구
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• 제12권2호
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• pp.183-190
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• 1985

식물생장억제제(植物生長抑制劑)인 Paclobutrazol이 수도(水稻)의 도복(倒伏)에 미치는 제형질(諸形質)과 수량(收量)에 미치는 영향(影響)을 조사(調査)하고자 낙동벼와 섬진벼를 공시하여 질소(窒素) 12,20kg/10a을 이용(利用)한 조건(條件)에서 Paclobutrazol 12,18g ai/10a을 출수전(出穗前) 20,15,10 일에 토양처리(土壤處理)하였다. 1. Paclobutrazol 18g ai/10a을 출수전(出穗前) 20일과 15일에 처리(處理)하면 수도(水稻)의 간장(稈長)은 전반적(全般的)으로 짧아졌으며, 특히 제(第) 3 절간(節間)에서 그 경향(傾向)이 현저하였다. 간장(稈長)은 Paclobutrazol 12,18g ai/10a을 출수전(出穗前) 20, 15일에 처리(處理)할 때 낙동벼에서 다소 짧아진 경향(傾向)이었다. 2. Paclobutrezol 처리(處理)에 의하여 간단면적(稈斷面積), linear density 및 줄기의 휨 강도 (EI)는 증가(增加)되었고 줄기가 만곡(彎曲)되는 경향(傾向)은 적었다. 3. 휨강도와 간단면적(稈斷面積), 간단축직경(稈短軸直徑) 및 linear density는 정(正)의 상관(相關), 줄기의 만곡각도(彎曲角度)와 간단면적(稈斷面積) 및 간단축직경(稈短軸直徑)과는 부(負)의 상관관계(相關關係)가 있었다. 4. Paclobutrazol 12g ai/10a 을 출수전(出穗前) 10, 15일에 처리(處理)하면 수량(收量)은 감소(減少)되지 않았으나 Paclobutrazol 18g ai/10a을 출수전(出穗前) 20일에 처리(處理)하면 수량(收量)은 약간 감소(減少)되는 경향(傾向)이었다.

• Journal of Acupuncture Research
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• 제29권4호
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• pp.43-53
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• 2012

Objectives : This study was accomplished to investigate the effects of moxibustion at Jeonjung($CV_{17}$) on the heart rate variability(HRV) in healthy adults. And based on that, we tried to find out how moxibustion at Jeonjung($CV_{17}$) effects on the activity and balance of the autonomic nervous system Methods : We investigated on 50 healthy volunteers consisted of 24 subjects in moxibustion group and 24 subjects in control group. On the other hand, two volunteers were ruled out by standard of elimination. The study was established by randomized trial. Moxibustion was applied at Jeonjung($CV_{17}$) and rested during 10 minutes in moxibustion group. No treatment was executed in control group. We measured HRV 3 times : baseline measurement and after treatment in moxibustion group(after rest in control group) twice. The SPSS 12.0 for windows was used to analyze the data by paired t-test, Wilcoxon signed ranks test(in group) and Independent t-test, Mann-Whitney U-test (between the groups). Results : 1. In moxibustion group, mean HRT decreased significantly. Ln(VLF) increased significantly. Ln(TP) decreased significantly at $2^{nd}$ time and increased significantly at $3^{rd}$ time. SDNN, RMSSD, PSI, Ln(LF), Ln(HF), Normalized LF, Normalized HF and LF/HF ratio were not affected. 2. In control group, PSI and normalized HF decreased significantly. SDNN, Ln(TP), Ln(VLF), normalized LF and LF/HF ratio increased significantly. Mean HRT, RMSSD, Ln(LF) and Ln(HF) were not affected. Conclusions : This study suggest that moxibustion at Jeonjung($CV_{17}$) makes no effects on the balance of the autonomic nervous system.

• Journal of Acupuncture Research
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• 제30권4호
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• pp.15-24
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• 2013

Objectives : This study was accomplished to investigate the effects of venesection with cupping therapy at Jeonjung($CV_{17}$) on the heart rate variability(HRV) in healthy adults. And based on that, we tried to find out how venesection with cupping therapy at Jeonjung($CV_{17}$) affects the activity and the balance of the autonomic nervous system. Methods : We investigated on 60 healthy volunteers consisted of 30 subjects in venesection with cupping therapy group and 30 subjects in control group. The study was established by randomized trial. venesection with cupping therapy was applied at Jeonjung($CV_{17}$) for 10 minutes in venesection with cupping therapy group. No treatment was executed in control group. We measured HRV 3 times : First, baseline measurement and two more times after treatment in venesection with cupping therapy group(after rest in control group). The SPSS 12.0 for Windows was used to analyze the data by paired t-test(in both group), independent-test(between the groups) and Chi-square test(between the sexs). Results : 1. In venesection with cupping therapy group, SDNN, Ln(TP), Ln(LF), Normalized LF, and LF/HF ratio increased significantly. Normalized HF decreased significantly. Mean HRT, RMSSD, PSI, Ln(HF), and Ln(VLF) were not affected. 2. In control group, Ln(HF) decreased significantly. Mean HRT, SDNN, RMSSD, PSI, Ln(TP), Ln(LF), Ln(VLF), Normalized LF, Normalized HF, and LF/HF ratio were not affected. Conclusions : This study suggests that venesection with cupping therapy at Jeonjung($CV_{17}$) increases the activity of the autonomic nervous system and has effects on the balance of the autonomic nervous system.

• 한국자원식물학회지
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• 제8권2호
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• pp.195-199
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• 1995

대두(大豆) 수량(收量)이 우수한 은하(銀河), 방사(放射), 팔달(八達) 재배품종(栽培品種)을 광합성활성폭사선(光合成活性幅射線) ((PAR) 범위(範圍)에서 5본엽기(本葉期)($V_5$)에 엽온(葉溫)을 달리하여 광합성속도(光合成速度)와 호흡속도(呼吸速度)의 변화를 측정하였고, 품종별(品種別) 엽(葉)의 특성인 비엽중(比葉重)(SLW), 엽녹소함양(葉綠素含量)과 광합성(光合成)과의 관계를 조사한 결과(結果)는 다음과 같다. 1. $25^{\circ}C$에서 순광합성속도(純光合成速度)(Net Photosynthesis)는 은하(銀河) $21.5mgdm^{-2}h^{-1}$, 방사(放射) $20.2mgdm^{-2}h^h{-1}$, 팔달(八達) $18.5mgdm^{-2}h^{-1}$이었다. 2. 전품종(全品種) 모두 엽온(葉溫) $25^{\circ}C$에서 광합성속도(光合成速度)가 가장 높았고 은하(銀河) 품종(品種)이 우수(優秀)했으며, 엽형(葉型)은 은하(銀河) (Long) > 방사(放射)(Oval) > 팔달(八達)(Round) 순서였다. 3. 엽(葉)의 호흡연도(呼吸連度)는 은하(銀河), 방사(放射), 팔달(八達)에서 $15^{\circ}$ $0.56mgdm^{-2}h^{-1}$, $20^{\circ}C$ $0.79mgdm^{-2}h^{-1}$, $25^{\circ}$ $1,15mgdm^{-2}h^{-1}$, $30^{\circ}C$ $1.37mgdm^{-2}h^{-1}$였다. 4. 비엽중(比葉重)은 방사(放射)가 $3.1mg/cm^2$이었고, 은하(銀河)와 팔달(八達)은 $3.5mg/cm^2$이었으며 비엽중(比葉重)과 광합성(光合成)과는 유의성(有意性)이 인정되지 않았다. 5. 엽녹소(葉綠素) 함량(含量)은 은하(銀河)가 $2.48{\mu}g/gF.W.$으로 가장 높았고, 방사(放射)가 $2.19{\mu}g/gF.W.$이었고 팔달(八達)이 $1.67{\mu}g/gF.W.$로 가장 낮았으며 엽록소(葉綠素) 함량(含量)과 광합성(光合成)과는 유의성(有意性)이 인정되었다. 6. 광보상점(光補償點)은 $15^{\circ}C$에서는 $10{\mu}Em^{-2}s^{-1}$로 모두 같았으나, $20^{\circ}C$에서 은하(銀河)는 $12{\mu}Em^{-2}s^{-1}$이었고, 방사(放射)와 팔달(八達)은 $13{\mu}Em^{-2}s^{-1}$이었다. $25^{\circ}C$에서는 은하(銀河)와 방사(放射)가 $16{\mu}Em^{-2}s^{-1}$이었지만 팔달은 $18{\mu}$m^{-2}s^{-1}$이었고, $30^{\circ}C$에서는 은하(銀河)와 방사(放射)가 $22{\mu}Em^{-2}s^{-1}$이었지만, 팔달은 $23{\mu}Em^{-2}s^{-1}$이었다.