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Effect of Daekumeumja Herb-acupuncture on Alcohol-induced Suppressed Cell Proliferation and Expression of Nitric Oxide Synthase in Hippocampus of Rats (대금음자(對金飮子) 약침이 알코올 독성 흰쥐의 해마에서 신경세포생성과 NOS 발현에 미치는 영향)

  • Kim, Hyun-Joong;Kim, Ee-Hwa;Lee, Eun-Yong
    • Journal of Acupuncture Research
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    • v.23 no.5
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    • pp.187-198
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    • 2006
  • Objectives : The purpose of this study is to assess the effect of Daekumeumja herb-acupuncture on neural cell proliferation and nitric oxide synthase(NOS) expression in hippocampus of ethanol-intoxicated Sprague-Dawley(SD) rats. Methods : SD rats were randomly assigned into 5 groups ; the normal group, the alcohol-treated(control) group, the alcohol- and 1 mg/kg Daekumeumja- treated(sample A) group, the alcohol- and 5mg/kg Daekumeumja-treated (sample B) group, the alcohol- and 10 mg/kg Daekumeumja-treated (sample C) group(n = 6 in each group). Normal group were received with Saline, while control group were injected intraperitoneally with alcohol(2 g/kg) once per day for 5 days. Sample groups were treated Daekumeumja herb-acupuncture on Chungwan(CV12) for 5 consecutive days. Bromo-deoxyuridine(BrdU) was injected into all animals once per day for 5 days. For the detection of BrdU-positive cells and NADPH-d- positive cells in hippocampus, immunohistochemistry was performed. Results : 1. In the dentate gyrus area, the number of BrdU-positive cells in the sample $B(278.08{\pm}6.46)$, $C(331.33{\pm}16.68)$ groups was significantly(p<0.05) increased compared with the control group. 2. In the dentate gyrus area, the number of NADPH-d-positive cells in the sample $C(86.50{\pm}10.02)$ groups was significantly(p<0.05) increased compared with the control group. 3. In the CAI area, the number of NADPH-d-positive cells in the sample $A(63.90{\pm}2.69)$, $B(75.70{\pm}3.01)$, $C(97.70{\pm}4.06)$ groups was significantly(p<0.05) increased compared with the control group. 4. In the CA 2-3 area, the number of NADPH-d-positive cells in the sample $B(30.20{\pm}1.89)$, $C(62.70{\pm}2.08)$ groups was significantly(p<0.05) increased compared with the control group. Conclusion : These results indicate that, neural cell proliferation and NOS expression in hippocampus was reduced in ethanol-intoxicated group. Treatment of Daekumeumja herb-acupuncture increased this diminution. Daekumeumja could be able to effect on the prevention of the amnesia and learning disability in alcoholism.

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Effect of Moxibustion on Patients with Idiopathic Parkinson's Disease (특발성 파킨슨병 환자의 뜸치료 효과)

  • Park, Sang-min;Lee, Sang-hoon;Kang, Mi-kyuang;Jung, Ji-cheol;Park, Hi-joon;Lim, Sabina;Chang, Dae-il;Lee, Yun-ho
    • Journal of Acupuncture Research
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    • v.22 no.1
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    • pp.91-97
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    • 2005
  • Objective : This study was designed to evaluate the effect of moxibustion with various scales on symptoms of idiopathic Parkinson's disease. Methods : Subjects were voluntarily recruited by newspapers and internet. All the subjects are confirmed as idiopathic parkinson's disease by a neurologist. The moxibustion therapy was performed 5 times a week by patient's family at home and once a week by oriental medical doctor at hospital. Moxibustion points were GV2O, CV12, ST36, BL18, BL2O. Intensity was up to pain threshold according to patients not to get burned. The patient's symptoms were assessed before, after 4 weeks and after 8 weeks treatment by unified Parkinson's disease rating scale(UPDRS), modified Hoehn-Yahr(H-Y) stage, Schwab & England activity of daily living and freezing of gait questionnaire(FOGQ). Results : Total UPDRS scores were significantly improved after 4 weeks(p<0.01) and after 8 weeks(p<0.01) compared to the pre-treatment. There were significant changes in H-Y stage after 4 weeks(p<0.05), but there were no significant changes in H-Y stage after 8 weeks. The scores of ADL were not significantly improved after 4 weeks(p>0.05) and after 8 weeks(p>0.05). There were significant changes in FOGQ scale after 4 weeks(p=0.05) and but there were no significant changes in FOGQ scale after 8 weeks(p=0.13). Conclusion : This study suggests that moxibustion treatments can be applicable to improve symptoms in the patients with idiopathic Parkinson's disease.

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Effects of Acupuncture Treatment on the Premenstrual Syndrome: Controlled Clinical Trial (월경전기증후군(月經前期症候群)(Premenstrual Syndrome)에 대한 침치료(鍼治療)의 효과(效果))

  • Kim, Sung-chul;Kim, Sung-nam;Lim, Jeong-a;Choi, chang-min;Sim, Eun-ki;Koo, Sung-tae;Lim, Jeong-a;Ha, Joo-young;Shin, Kyung-rim;Sohn, In-chuI
    • Journal of Acupuncture Research
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    • v.22 no.1
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    • pp.41-60
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    • 2005
  • Objective : The purpose of the present study was to evaluate the effect of acupuncture therapy on the premenstrual syndrome. Methods : Volunteers of twenty subjects were employed using DRSP questionnaire. Subjects were divided into two groups including active-acupuncture treatment group, placebo acupuncture treatment group. In the control group, subjects were needled at $SI_5$, $ST_{40}$ points and in the acupuncture group, subjects were stimulated at two basic points, $SP_6$ and $CV_6$, and several points were inserted additionally depending the symptoms including $LR_2$, $LR_3$, $SP_10$, $LI_4$, and $ST_3$. A total of 13 acupuncture sessions were performed for each patient depending on the individual menstruation cycle over 8 weeks. Concentration of blood progesterone was examined and Digital Infrared Thermal Imaging (DITI) was taken before and after acupuncture treatment.

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A Study on Depression with Acupuncture & Moxibustion Traetment in Chinese Medical Literature (우울증(憂鬱症)의 침구치료(鍼灸治療)에 관(關)한 중의문헌(中醫文獻)의 고찰(考察))

  • Kim, Yeo-jin;Park, Dong-suk;Lee, Yun-ho
    • Journal of Acupuncture Research
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    • v.22 no.1
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    • pp.223-234
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    • 2005
  • Objective : The objective of this study was to research depression with acupuncture & moxibustion treatment. Methods : We searched Chinese Medical Literature(published from 1985~2002) related to depression and acupuncture. Results : 1) PC6(內關), HT7(神門), ST36(足三里), GV20(百會), LR3(太衝), SP6(三陰交) are much used for the treatment depression. 2. LR3(太衝), CV17(전중), GB34(陽陵泉), ST40(豊隆), PC6(內關) are much used for the excess type of depression and PC6(內關), SP6(三陰交), HT7(神門), BL15(心兪) are much used for deficiency type of depression. 3. ST36(足三里), SP6(三稜交), HT7(神門), PC6(內關), HT5(通里), KI1(通泉) are used for the treatment depression in electroacupuncture therapy. BL15(心兪), BL20(脾兪),ST36(足三里) are used for the treatment depression in acupoint injection therapy. 4. In dermal needle therapy, it is used to tap slightly until the skin becomes congested in the region of Bladder meridian and nape, back of Governor Vessel. 5. It seems that psychotherapy, music therapy and light therapy will be applied for the treatment of depression. Conclusion : We expect that acupuncture treatment of depression will be applied practically in clinical medicine due to further study on depression with acupuncture.

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Coolant Leak Effect on Polymer Electrolyte Membrane Fuel Cell (고분자전해질연료전지의 냉각수 누설에 대한 연구)

  • Song, Hyun-Do;Kang, Jung-Tak;Kim, Jun-Bom
    • Journal of the Korean Electrochemical Society
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    • v.10 no.4
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    • pp.301-305
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    • 2007
  • The performance of polymer electrolyte membrane fuel cell could be decreased due to coolant leaked from connection part. Micro pump was used to put small amount of coolant and investigate the effect on fuel cell. The stoichiometric ratio of hydrogen/air was 1.5/2.0, both side of gas was fully humidified, and current density of $400mA/cm^2$ was used as standard condition in this experiment. Constant current method was used to check performance recovery from coolant effect in 3 cell stack. The performance was recovered when coolant was injected in cathode side. On the other hand, the performance was not recovered when coolant was injected in anode side. Ethylene glycol could be converted to CO in oxidation process and cause poisoning effect on platinum catalyst or be adhered on GDL and cause gas diffusion block effect resulting performance decrease. Water with nitrogen gas was supplied in anode side to check performance recovery. Polarization curve, cyclic voltammetry, electrochemical impedance spectroscopy was used to check performance, and gas chromatography was used to check coolant concentration. Constant current method was not enough in full recovery of performance. However, water injection method was proved good method in full recovery of performance.

The Effects of Gamma Irradiation on the Microbiological, Physicochemical and Sensory Quality of Peach (Prunus persica L. Batsch cv Dangeumdo) (감마선 조사에 의한 복숭아의 미생물학적, 이화학적 품질 및 관능적 품질 변화)

  • Kim, Mi-Seon;Kim, Kyoung-Hee;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.3
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    • pp.364-371
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    • 2009
  • The effect of gamma irradiation ($0.5{\sim}2$ kGy) on the microbiological, physicochemical and sensory properties of peaches was investigated during 6 day storage at $20{\pm}3^{\circ}C$. Total aerobic bacteria, yeasts and molds significantly decreased with increasing dose level. In Hunter's color values, L-values decreased and a- and b-values increased with increment of irradiation dose level. Hardness significantly decreased with increment of irradiation dose level whereas pH, soluble solid, and total polyphenol contents increased with increment of irradiation dose level. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity of irradiated peach was higher than that of control and its activity increased with increment of irradiation dose level. Vitamin C content was not affected by irradiation. In sensory test, overall acceptabilities of irradiated samples were higher than those of control. These results suggest that gamma irradiation on peach was effective for microbiological safety while improving the antioxidant activity, but not good on color and texture of peach.

Antioxidant Activity from Different Root Parts of 6-year-old Panax ginseng C.A. Meyer (Yun-poong) (6년생 인삼(연풍)의 뿌리부위별 항산화 활성)

  • Jo, Ji-Eun;Kim, Kyoung-Hee;Kim, Mi-Seon;Choi, Jae-Eul;Byun, Myung-Woo;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.4
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    • pp.493-499
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    • 2011
  • The objective of this study was to evaluate the contents of reducing sugar and total polyphenol and the antioxidant activity of freeze-dried ginseng (Panax ginseng C.A. meyer cv Yun-poong). Ginseng root consists of the main root (MR), lateral root (LR) and fine root (FR). These roots were sorted into 3~4 groups (MR 1~4 groups, LR 1~4 groups, FR 1~3 groups) depending on the diameter of center region. The reducing sugar content has no influence on the diameter, but the reducing sugar content was decreased by subdivision. Total polyphenol contents of ginseng were the highest in FR-3 among all others. The antioxidant activity was measured using DPPH radical scavenging activity and ABTS radical scavenging activity. The $IC_{50}$ (50% inhibitory concentration) value of the hydrogen donating activity was the highest in FR-3 as 7.03 mg/mL and the lowest in MR-2 as 37.02 mg/mL. Overall, hydrogen donating activity of FR was higher than the main root and lateral root significantly (p<0.05). The ABTS radical scavenging activity in FR-3 showed the highest radical scavenging activity as 25.49%. This study's findings suggest that the total polyphenol contents and antioxidant activity of ginseng were the highest in FR-3, and that polyphenol contents and antioxidant activity of ginseng were related to root area and diameter.

Effects of Electrolyte Concentration on Growth of Dendritic Zinc in Aqueous Solutions (수용액중 아연 덴드라이트의 성장 반응에 미치는 전해질 농도의 영향)

  • Shin, Kyung-Hee;Jung, Kyu-Nam;Yoon, Su-Keun;Yeon, Sun-Hwa;Shim, Joon-Mok;Joen, Jae-Deok;Jin, Chang-Soo;Kim, Yang-Soo;Park, Kyoung-Soo;Jeong, Soon-Ki
    • Transactions of the Korean hydrogen and new energy society
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    • v.23 no.4
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    • pp.390-396
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    • 2012
  • In order to understand the nature of dendritic zinc growth, electrochemical zinc redox reaction on nickel plate was investigated in aqueous solutions containing different concentrations, 0.2, 0.1 and 0.02 $mol{\cdot}dm^{-3}$ (M), of zinc sulfate ($ZnSO_4$) or zinc chloride ($ZnCl_2$). Zinc ion was efficiently reduced and oxidized on nickel in the high-concentration (0.2 M) solution, whereas relatively poor efficiency was obtained from the other low-concentration solutions (0,1 and 0.02 M). Cyclic voltammetry (CV) analysis revealed that the 0.2 M electrolyte solution decomposes at more positive potentials than the 0.1 and the 0.02 M solutions. These results suggested that the concentration of electrolyte solution and anion would be an important factor that suppresses the reaction of the zinc dendrite formation. Scanning Electron Microscopy (SEM) data revealed that the shape of dendritic zinc and its growing behavior were also influenced by electrolyte concentration.

Effect of Dikegulac and Ancymidol on Growth and Fowering of Salvia splendens 'Bonfire' (Salvia의 생장(生長)과 개화(開花)에 미치는 Dikegulac과 Ancymidol의 효과(效果))

  • Lee, Jong Suk
    • Korean Journal of Agricultural Science
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    • v.6 no.1
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    • pp.21-26
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    • 1979
  • To compare the effect of dikegulac (sodium 2,3 : 4, 6-di-O-isopropylidene-2-keto-L-gulonate) and ancymidol (${\alpha}$-cyclopropyl-${\alpha}$-(4-methoxyphenyl)-5-pyrimidinemethanol) on the growth and flowering responses of Salvia splendens cv. Bonfire, foliar spray of dikegulac at rates of 0.06 and 0.12% and ancymidol at rates of 50 and 100 ppm, and soil drench of ancymidol at rates of 0.1 and 0.5 mg/pot were treated. Dikegulac reduced the plant height and induced the lateral shoot development by disrupting apical dominance, and thus increased the number of inflorescences and retarded flowering date. Dikegulac treatment greatly increased chlorophyll content of leaf, especially chlorophyll a. Ancymidol reduced the plant height without disrupting apical dominance and retarding flower ing date, but did not increase inflorescence number except soil drench at rate of 0.1 mg/pct. Ancymidol treatment significantly increased chlorophyll content of leaf, especially chlorophyll a. Dikegulac can be effectively used as a pinching agent and branching agent for salvia.

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Comparison of Labour and Growth Characters of Grape cv. 'Campbell Early' between Wakeman and Modified-T Trellis Training Systems (포도 '캠벨얼리'의 웨이크만과 개량일자형 수형에서의 노동력과 생장특성 비교)

  • Park, Seo-Jun;Cho, Eun-Kyung;Kim, Su-Jin;Hur, Youn-Young;Nam, Jong-Chul;Park, Jeong-Kwan;Hwang, Hae-Sung;Jung, Sung-Min
    • Journal of Bio-Environment Control
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    • v.25 no.3
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    • pp.206-211
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    • 2016
  • Modified T trellis (MT) and Wakeman trellis (WT) were widely used Korean vineyard because they had an advantage for spur pruning type cultivar such as Campbell Early. In this experiment, we compared labor time and intensity for bunch management between MT and WT trellis systems in 'Campbell Early' grapes. As a result, berry thinning was required 17.3 hours (10a) on the WT trellis but was required 12.3 hours (10a) on the MT trellis. In like manner, bagging was required 10.1 hours (10a) on the WT trellis but was required 8.2 hours (10a) on the MT trellis. On the other hand, labor intensity measured on berry thinning and bagging practices using REBA (Rapid Entire Body Assessment) index, then WT trellis was scored 13.0, but MT trellis was scored 8.6. Meanwhile, MT trellis reduced vigorous growing of internodes length and width on grapevine shoots. Consequently, MT trellis is more convenience trellis for working ergonomically in Korea vineyard.