• 한국응용곤충학회지
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• 제53권4호
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• pp.331-338
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• 2014

시스타틴(cystatin: CST)은 C1A류 시스테인 단백질분해효소에 대한 경쟁적 가역억제자로서 동식물류에서 파파인과 같은 캐셉신을 억제대상으로 작용하게 된다. 바이러스 유래 CST (CpBV-CST1)이 폴리드나바이러스의 일종인 CpBV (Cotesia plutellae bracovirus)에서 동정되었다. 기존 연구는 이 유전자의 과발현이 배추좀나방(Plutella xylostella) 유충의 면역 및 발육을 교란한다는 것을 보여 주었다. 본 연구는 이 유전자의 단백질 기능을 분석하기 위해 세균발현시스템을 이용하여 재조합단백질(rCpBV-CST1)을 형성하여 단백질분해효소에 대한 활성억제효과를 결정하고, 곤충의 면역과 발육에 대한 생리적 억제효과를 분석했다. 이 유전자 번역부위는 138 개 아미노산으로 약 15 kDa 크기의 단백질로 추정되었다. CpBV-CST1이 먼저 pGEX 발현벡터에 재조합되고, BL21 STAR (DE3) competent cells에 형질전환된 후 0.5 mM IPTG로 4 시간동안 과발현되었다. 분리된 재조합단백질은 파파인에 대한 뚜렷한 억제효과를 나타냈다. 이 재조합단백질은 파밤나방(Spodoptera exigua)에 대해서 혈구소낭형성의 세포성 면역반응을 억제하고, 경구로 처리할 때 배추좀나방의 유충발육을 처리 농도에 비례하여 제한시켰다. 이상의 결과는 CpBV-CST1이 해충 밀도 억제에 응용될 수 있음을 제시하고 있다.

• 대한한방체열의학회지
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• 제6권1호
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• pp.69-75
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• 2008

Purpose: We investigated the effect of far-infrared radiating products on cold hypersensitivity of lower limbs using CST. Methods: 7 patients with cold hypersensitivity of lower limbs were investigated in this study. Exclusive criteria was skin diseases, spinal nervous disease and external wounds. They were asked to answer the VAS of cold hypersensitivity at baseline and after 3 weeks. We measured temperature of lower limbs with Spectrum 9000 MB (Dorex Inc, USA). We performed cold stress test (CST) by 3 thermographic observation using DITI : 1st was taken after 15 minutes resting at $25^{\circ}C$, the 2nd was immediately taken after 1 minute soak in $20^{\circ}C$ water, the 3rd was taken at 15 minutes after soak. We performed 3 times of CST : 1st CST was perfomed at baseline, 2nd CST was perfomred after 1 week and just observation, 3rd CST was performed after 1 week using far-infrared radiating products (lasner, UMT, Korea). Results: After using products, temperature of foot incresed more than thigh area, but there was no significance. There was no statistical difference of VAS, change of temperature and CST between before and after using far-infrared radiating products. Conclusion: There was no statistical effect of far-infrared radiating products on change of temperature of lower limbs.

• 한국산학기술학회논문지
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• 제17권1호
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• pp.474-484
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• 2016

최근 CST 효과에 대한 많은 과학적 근거들이 활발히 제시되고 있으며, CST를 실제 임상에서 적극적으로 활용하고 있다. 이에 본 연구는 CST가 장기요양시설노인의 뇌기능 향상에 효과가 있는지를 확인하기 위한 비동등성 대조군 전후 설계의 유사 실험연구이다. 2015년 1월 3일~2015년 2월 28일까지 장기요양시설노인 12명(실험군 6명, 대조군 6명)을 대상으로 Upledger가 개발한 10단계(10-Step Protocol) CST를 1회 50분, 주 1회, 8주간, 총 8회 적용하였다. 뇌기능 측정은 2 Channel neuro-feedback system 이동식 뇌파 측정기를 이용하여, CST 시술 시작 전과 마지막 시술 후에 뇌파를 측정하였으며, 한국정신과학연구소에서 개발한 뇌기능 분석 프로그램인 BQ test로 뇌기능지수를 분석하였다. 집단 간 자료의 분석은 SPSS Windows(Version 18.0)프로그램의 Mann-Whitney U-test를 사용하였다. 연구결과 뇌기능지수 중 주의지수(좌)(우), 주의지수의 하위지수인 긴장도(좌)(우), 항스트레스지수(좌)(우), 정서지수, 브레인지수에서 유의한 변화가 나타났고, 주의지수의 하위 지수인 주의비율에서만 유의하지 않았다. 이상의 결과로 CST가 시설노인의 뇌기능지수를 증가시켜 뇌기능을 향상시켰음을 확인할 수 있었으며, CST를 노인요양시설에서 건강과 뇌기능 향상을 위한 효과적인 중재방법으로 활용할 것을 제안한다.

• Journal of Korean Neurosurgical Society
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• 제55권5호
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• pp.267-272
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• 2014

Objective : Differentiation of demyelination in white matter from axonal damage can be determined using diffusion tensor imaging (DTI). In this study using meningioma patients an attempt was made to evaluate the relationship between preoperative weakness and the changes of diffusion parameters in the corticospinal tract (CST) using DTI. Methods : Twenty-six patients with meningioma were enrolled in this study. Eleven of them suffered from objective motor weakness and were classified as Group 1. The remaining 15 patients did not present motor weakness and were classified as Group 2. Fiber tractography and CST diffusion parameters were obtained using DTIStudio. The ratios (lesion side mean value/contralateral side mean value) of CST diffusion parameters were compared with 1.0 as a test value using a one-sample t-test. Results : In Group 1, fractional anisotropy (FA), tensor trace (TT), and radial diffusivity (RD, ${\lambda}2$ and ${\lambda}3$) of the CST were significantly different between two hemispheres, but axial diffusivity (AD, ${\lambda}1$) of the CST was not significantly different between two hemispheres. In Group 2, FA and ${\lambda}3$ of CST did not differ significantly between the hemispheres. In Group 2, TT, ${\lambda}1$, and ${\lambda}2$ of CST in the ipsilateral hemisphere were significantly higher than those of the unaffected hemisphere. However, the differences were small. Conclusion : Motor weakness was related to a low FA and high TT resulting from increased RD of the CST fibers. CST diffusion changes in patients with weakness are similar to those for demyelination.

• 혜화의학회지
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• 제20권2호
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• pp.41-52
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• 2012

In vitro tests were performed using CST to investigate its role on oxidative damages and inflammatory cytokines. 90% or higher cell viability was observed in CST treated groups from 25 to 200 ${\mu}g/m{\ell}$ using Raw 264.7 cells. CST showed dose-dependent DPPH scavenging activity, with 91.3% and 92.2% scavenging activities at 400 and 800 ${\mu}g/m{\ell}$ concentrations, respectively. CST showed dose-dependent suppression activity of ROS production, especially at 200 ${\mu}g/m{\ell}$ of 41.3%. CST decreased NO production activity, with significant decrease of 16.2% and 33.5% at 100 and 200 ${\mu}g/m{\ell}$ concentrations, respectively. IL-$1{\beta}$, IL-6, MCP-1 production rate were significantly decreased by 30.0%, 27.2%, 22.1% when Raw 264.7 cells were treated with LPS and with CST of 200 ${\mu}g/m{\ell}$. Also, TNF-${\alpha}$ production rate was decreased by 28.6%. The results above indicated therapeutic effect of CST on the AD through anti-oxidative and immune modulatory effect. Various blending of drug substances with CST should be clinically tested.

• Journal of Forest and Environmental Science
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• 제30권1호
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• pp.85-90
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• 2014

Dewaterability, one of the important properties of wastewater sludge, was investigated using a simple capillary suction time (CST) measurement method. CST and SRF have a very close co-relation. It was convinced that CST method was quite effective, and compensating the time-consuming SRF of conventional drainage measuring method. It turned out that one could use the results of CST to find optimum flocculants ratio to improve drainage in wastewater treatment for the tissue paper production at a mill. Since the optimum ratio of flocculants could be determined with the value of CST and COD removal efficiency could be improved with precise ratio of flocculants. Thus, using CST for determining the optimum ratio of flocculants could be economical by reducing the amount of flocculants. Dewaterability might be measured within several seconds using the values of CST in a precise way. The dewaterability could also be useful in investigating the optimum ratio of flocculants.

• 대한후두음성언어의학회지
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• 제33권1호
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• pp.26-30
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• 2022

Background and Objectives Cough suppression therapy (CST) is a physiotherapy that can be used for patients with chronic refractory cough (CRC). We aimed to investigate the efficacy of CST for CRC. Materials and Method A prospective randomized controlled trial was conducted in 27 patients with CRC. Participants were randomized to receive either standard mucolytic medications for CRC combined with supplemental CST (CST group) or standard medications alone (control group). CST consists of laryngeal hygiene management, humidification, cough suppression technique, breathing method, and counseling. We assessed the symptoms change at baseline and week 4 with the Leicester Cough Questionnaire (LCQ). Secondary efficacy outcomes included the degree of cough Visual Analog Scale (VAS) score (0 to 100 scale). Results From 2019 to 2021, 14 CST group patients and 13 control group patients were included. The improvement was significantly greater in the CST group than in the control group for cough VAS score (36.67 to 13.33 vs. 74.29 to 16.43, p<0.001). Patients in the CST group had a significant improvement in total (70.14 to 107.71, p=0.005), physical (31.42 to 43.86, p=0.015), psychological (23.57 to 40.14, p=0.003), and social (15.14 to 23.71, p=0.005) LCQ scores. However, there was not a significant pre- to post-treatment LCQ score improvement in control group. Conclusion CST might be an effective supplemental intervention for CRC.

• 펄프종이기술
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• 제40권1호
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• pp.41-46
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• 2008

In this study, dewaterability, one of the important properties of wastewater sludge, was investigated using a simple capillary suction time (CST) measurement method. It turned out that one could use the results of CST to find optimum flocculants ratio to improve drainage in wastewater treatment for the printing paper, tissue paper, and newsprint paper mill. Since the optimum ratio of flocculants could be determined with the value of CST, COD removal efficiency could be improved and optimum floc strength could be achieved with precise ratio of flocculants. It was thus that using CST for determining the optimum ratio of flocculants could be economical by reducing the amount of flocculants. Dewaterability could be measured within several seconds using the values of CST in a precise way. The dewaterability could also be useful in investigating the optimum ratio of flocculants.

• 한국축산식품학회지
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• 제43권5호
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• pp.877-888
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• 2023

We studied the proteolysis and conducted a sensory evaluation of fermented sausages using strains derived from Kimchi [Pediococcus pentosaceus-SMFM2021-GK1 (GK1); P. pentosaceus-SMFM2021-NK3 (NK3)], Doenjang [Debaryomyces hansenii-SMFM2021-D1 (D1)], and spontaneous fermented sausage [Penicillium nalgiovense-SMFM2021-S6 (S6)]. Fermented sausages were classified as commercial starter culture (CST), mixed with GK1, D1, and S6 (GKDS), and mixed with NK3, D1, and S6 (NKDS). The protein content and pH of GKDS and NKDS were significantly higher than those of CST on days 3 and 31, respectively (p<0.05). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the NKDS had higher molecular weight proteins than the GKDS and CST. The myofibrillar protein solubility of the GKDS and NKDS was significantly higher than that of the CST on day 31 (p<0.05). The GKDS displayed significantly higher pepsin and trypsin digestion than the NKDS on day 31 (p<0.05). The hardness, chewiness, gumminess, and cohesiveness of the GKDS were not significantly different from those of the CST. The GKDS exhibited the highest values for flavor, tenderness, texture, and overall acceptability. According to this study, sausages fermented using lactic acid bacteria (GK1), yeast (D1), and mold (S6) derived from Korean fermented foods displayed high proteolysis and excellent sensory evaluation results.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2010년도 추계 학술발표회 3차
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• pp.140-145
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• 2010

Consolidation in cohesive soils mainly focuses on compressibility of soils, but it affects solute transport in some cases. The consolidation process takes on particular significance for fine grained soils at high water content, such as dredged sediments, but has also been shown to be important for compacted clay liners during waste filling operation. Numerical investigation using CST1 and CST2 was reviewed on consolidation-induced solute transport in this paper, especially with the development of CST2 model, verification by comparing experimental results with numerical simulations, and cases studies regarding transport in a confined disposal facility (CDF) and during in-situ capping. The importance of the consolidation process on solute transport is accessed based on simulated concentration or mass breakthrough curves. Results indicate that neglecting transient consolidation effects may lead to significant errors in transport analyses, especially with soft contaminated cohesive soils undergoing large volume change.