• Korean journal of applied entomology
• /
• v.53 no.4
• /
• pp.331-338
• /
• 2014

Cystatins (CSTs) are reversible and competitive inhibitors of C1A cysteine proteases, corresponding to papain-like cathepsins in plants and animals. A viral CST (CpBV-CST1) was identified from a polydnavirus, Cotesia plutellae bracovirus (CpBV). Our previous study indicated that a transient expression of CpBV-CST1 interfered with immune response and development of Plutella xylostella larvae. To directly demonstrate the protein function, this study produced a recombinant CpBV-CST1 protein (rCpBV-CST1) using bacterial expression system to determine its inhibitory activity against cysteine protease and to assess its physiological alteration in insect immune and development. The open reading frame of CpBV-CST1 encodes a polypeptide of 138 amino acids (${\approx}15kDa$). rCpBV-cystatin protein in BL21 STAR (DE3) competent cells containing a recombinant pGEX4T-3:CpBV-CST1 was over-expressed by 0.5 mM IPTG for 4 h. In biological activity assay, the purified rCpBV-CST1 showed a significant inhibition against papain activity. It inhibited a cellular immune response of hemocyte nodule formation in the beet armyworm, Spodoptera exigua. Moreover, its oral administration retarded larval development of the diamondback moth, Plutella xylostella in a dose-dependent manner. These results suggest that CpBV-CST1 may be applied to control insect pest populations.

• Journal of Oriental Medical Thermology
• /
• v.6 no.1
• /
• pp.69-75
• /
• 2008

Purpose: We investigated the effect of far-infrared radiating products on cold hypersensitivity of lower limbs using CST. Methods: 7 patients with cold hypersensitivity of lower limbs were investigated in this study. Exclusive criteria was skin diseases, spinal nervous disease and external wounds. They were asked to answer the VAS of cold hypersensitivity at baseline and after 3 weeks. We measured temperature of lower limbs with Spectrum 9000 MB (Dorex Inc, USA). We performed cold stress test (CST) by 3 thermographic observation using DITI : 1st was taken after 15 minutes resting at $25^{\circ}C$, the 2nd was immediately taken after 1 minute soak in $20^{\circ}C$ water, the 3rd was taken at 15 minutes after soak. We performed 3 times of CST : 1st CST was perfomed at baseline, 2nd CST was perfomred after 1 week and just observation, 3rd CST was performed after 1 week using far-infrared radiating products (lasner, UMT, Korea). Results: After using products, temperature of foot incresed more than thigh area, but there was no significance. There was no statistical difference of VAS, change of temperature and CST between before and after using far-infrared radiating products. Conclusion: There was no statistical effect of far-infrared radiating products on change of temperature of lower limbs.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.17 no.1
• /
• pp.474-484
• /
• 2016

This study was performed to identify the effects of the improvement of brain function by CST on the elderly with the 2nd or 3rd grade of long-term care insurance service. A quasi-experimental design using a nonequivalent control group, pre-post test was used. A total of 12 elders (6 in the experimental group and 6 in the control group) were recruited. Upledger CST(10-Step Protocol) was performed on each subject for 50 minutes per session, once a week, for a total of 8 treatments over an 8 week period from Jan to Feb 2015. The brain function quotient was measured before the 1st CST and after the last CST by portable EEG measurement device using a 2 Channel neuro-feedback system. The data was analyzed by SPSS (Ver. 18.0) program. After CST intervention, the attention quotient (AQ), level of tension, anti-stress quotient (ASQ), emotion quotient (EQ) and brain quotient (BQ) of the experimental group was significantly better than that of the control group. These results showed that the CST was effective in reducing the level of fatigue by the AQ, increasing the physical and psychological stress relief by the ASQ, emotional balance by the EQ, and improving the total brain function by the BQ. Therefore, CST can be used as an effective intervention for improving the health and brain function of the elderly in health facilities.

• Journal of Korean Neurosurgical Society
• /
• v.55 no.5
• /
• pp.267-272
• /
• 2014

Objective : Differentiation of demyelination in white matter from axonal damage can be determined using diffusion tensor imaging (DTI). In this study using meningioma patients an attempt was made to evaluate the relationship between preoperative weakness and the changes of diffusion parameters in the corticospinal tract (CST) using DTI. Methods : Twenty-six patients with meningioma were enrolled in this study. Eleven of them suffered from objective motor weakness and were classified as Group 1. The remaining 15 patients did not present motor weakness and were classified as Group 2. Fiber tractography and CST diffusion parameters were obtained using DTIStudio. The ratios (lesion side mean value/contralateral side mean value) of CST diffusion parameters were compared with 1.0 as a test value using a one-sample t-test. Results : In Group 1, fractional anisotropy (FA), tensor trace (TT), and radial diffusivity (RD, ${\lambda}2$ and ${\lambda}3$) of the CST were significantly different between two hemispheres, but axial diffusivity (AD, ${\lambda}1$) of the CST was not significantly different between two hemispheres. In Group 2, FA and ${\lambda}3$ of CST did not differ significantly between the hemispheres. In Group 2, TT, ${\lambda}1$, and ${\lambda}2$ of CST in the ipsilateral hemisphere were significantly higher than those of the unaffected hemisphere. However, the differences were small. Conclusion : Motor weakness was related to a low FA and high TT resulting from increased RD of the CST fibers. CST diffusion changes in patients with weakness are similar to those for demyelination.

• Journal of Haehwa Medicine
• /
• v.20 no.2
• /
• pp.41-52
• /
• 2012

In vitro tests were performed using CST to investigate its role on oxidative damages and inflammatory cytokines. 90% or higher cell viability was observed in CST treated groups from 25 to 200 ${\mu}g/m{\ell}$ using Raw 264.7 cells. CST showed dose-dependent DPPH scavenging activity, with 91.3% and 92.2% scavenging activities at 400 and 800 ${\mu}g/m{\ell}$ concentrations, respectively. CST showed dose-dependent suppression activity of ROS production, especially at 200 ${\mu}g/m{\ell}$ of 41.3%. CST decreased NO production activity, with significant decrease of 16.2% and 33.5% at 100 and 200 ${\mu}g/m{\ell}$ concentrations, respectively. IL-$1{\beta}$, IL-6, MCP-1 production rate were significantly decreased by 30.0%, 27.2%, 22.1% when Raw 264.7 cells were treated with LPS and with CST of 200 ${\mu}g/m{\ell}$. Also, TNF-${\alpha}$ production rate was decreased by 28.6%. The results above indicated therapeutic effect of CST on the AD through anti-oxidative and immune modulatory effect. Various blending of drug substances with CST should be clinically tested.

• Journal of Forest and Environmental Science
• /
• v.30 no.1
• /
• pp.85-90
• /
• 2014

Dewaterability, one of the important properties of wastewater sludge, was investigated using a simple capillary suction time (CST) measurement method. CST and SRF have a very close co-relation. It was convinced that CST method was quite effective, and compensating the time-consuming SRF of conventional drainage measuring method. It turned out that one could use the results of CST to find optimum flocculants ratio to improve drainage in wastewater treatment for the tissue paper production at a mill. Since the optimum ratio of flocculants could be determined with the value of CST and COD removal efficiency could be improved with precise ratio of flocculants. Thus, using CST for determining the optimum ratio of flocculants could be economical by reducing the amount of flocculants. Dewaterability might be measured within several seconds using the values of CST in a precise way. The dewaterability could also be useful in investigating the optimum ratio of flocculants.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
• /
• v.33 no.1
• /
• pp.26-30
• /
• 2022

Background and Objectives Cough suppression therapy (CST) is a physiotherapy that can be used for patients with chronic refractory cough (CRC). We aimed to investigate the efficacy of CST for CRC. Materials and Method A prospective randomized controlled trial was conducted in 27 patients with CRC. Participants were randomized to receive either standard mucolytic medications for CRC combined with supplemental CST (CST group) or standard medications alone (control group). CST consists of laryngeal hygiene management, humidification, cough suppression technique, breathing method, and counseling. We assessed the symptoms change at baseline and week 4 with the Leicester Cough Questionnaire (LCQ). Secondary efficacy outcomes included the degree of cough Visual Analog Scale (VAS) score (0 to 100 scale). Results From 2019 to 2021, 14 CST group patients and 13 control group patients were included. The improvement was significantly greater in the CST group than in the control group for cough VAS score (36.67 to 13.33 vs. 74.29 to 16.43, p<0.001). Patients in the CST group had a significant improvement in total (70.14 to 107.71, p=0.005), physical (31.42 to 43.86, p=0.015), psychological (23.57 to 40.14, p=0.003), and social (15.14 to 23.71, p=0.005) LCQ scores. However, there was not a significant pre- to post-treatment LCQ score improvement in control group. Conclusion CST might be an effective supplemental intervention for CRC.

• Journal of Korea Technical Association of The Pulp and Paper Industry
• /
• v.40 no.1
• /
• pp.41-46
• /
• 2008

In this study, dewaterability, one of the important properties of wastewater sludge, was investigated using a simple capillary suction time (CST) measurement method. It turned out that one could use the results of CST to find optimum flocculants ratio to improve drainage in wastewater treatment for the printing paper, tissue paper, and newsprint paper mill. Since the optimum ratio of flocculants could be determined with the value of CST, COD removal efficiency could be improved and optimum floc strength could be achieved with precise ratio of flocculants. It was thus that using CST for determining the optimum ratio of flocculants could be economical by reducing the amount of flocculants. Dewaterability could be measured within several seconds using the values of CST in a precise way. The dewaterability could also be useful in investigating the optimum ratio of flocculants.

• Food Science of Animal Resources
• /
• v.43 no.5
• /
• pp.877-888
• /
• 2023

We studied the proteolysis and conducted a sensory evaluation of fermented sausages using strains derived from Kimchi [Pediococcus pentosaceus-SMFM2021-GK1 (GK1); P. pentosaceus-SMFM2021-NK3 (NK3)], Doenjang [Debaryomyces hansenii-SMFM2021-D1 (D1)], and spontaneous fermented sausage [Penicillium nalgiovense-SMFM2021-S6 (S6)]. Fermented sausages were classified as commercial starter culture (CST), mixed with GK1, D1, and S6 (GKDS), and mixed with NK3, D1, and S6 (NKDS). The protein content and pH of GKDS and NKDS were significantly higher than those of CST on days 3 and 31, respectively (p<0.05). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the NKDS had higher molecular weight proteins than the GKDS and CST. The myofibrillar protein solubility of the GKDS and NKDS was significantly higher than that of the CST on day 31 (p<0.05). The GKDS displayed significantly higher pepsin and trypsin digestion than the NKDS on day 31 (p<0.05). The hardness, chewiness, gumminess, and cohesiveness of the GKDS were not significantly different from those of the CST. The GKDS exhibited the highest values for flavor, tenderness, texture, and overall acceptability. According to this study, sausages fermented using lactic acid bacteria (GK1), yeast (D1), and mold (S6) derived from Korean fermented foods displayed high proteolysis and excellent sensory evaluation results.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2010.09c
• /
• pp.140-145
• /
• 2010

Consolidation in cohesive soils mainly focuses on compressibility of soils, but it affects solute transport in some cases. The consolidation process takes on particular significance for fine grained soils at high water content, such as dredged sediments, but has also been shown to be important for compacted clay liners during waste filling operation. Numerical investigation using CST1 and CST2 was reviewed on consolidation-induced solute transport in this paper, especially with the development of CST2 model, verification by comparing experimental results with numerical simulations, and cases studies regarding transport in a confined disposal facility (CDF) and during in-situ capping. The importance of the consolidation process on solute transport is accessed based on simulated concentration or mass breakthrough curves. Results indicate that neglecting transient consolidation effects may lead to significant errors in transport analyses, especially with soft contaminated cohesive soils undergoing large volume change.