• 무역상무연구
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• 제47권
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• pp.239-260
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• 2010

The purpose of this study would like to investigate the critical success factors of the e-procurement which means on-line purchase of goods and services for organizations. This action represents one of the largest expense items in a firm's cost structure. In order to draw CSFs of e-procurement system, we have surveyed questionnaire to the related firms. The basic variables used in this survey are taken from many precedent researches in relation to the e-procurement. On the other hand, the research data is collected from companies which had used the MRO e-marketplace. Through factor analysis of those data, four-factor resulted as follows: (1) factor one suggests the rationalization of the firm's management of its suppliers; (2) factor two calls for redesigning affected business processes and influencing end-user/employee procurement-related behaviors; (3) factor three refers to carefully orchestrating an e-procurement technology planning process with one's suppliers and using intelligence in designing the software and mining the data it produces; and (4) factor four relates to selecting an e-procurement solution and/or simultaneously participating in a number of electronic environments supporting e-procurement. In conclusion, these four factors are regarded as critical success factors for the application of e-procurement system and then suppliers should take care the effective planning of e-procurement structure.

• 디지털융복합연구
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• 제10권3호
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• pp.51-58
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• 2012

본 연구에서는 경영혁신전략의 하나로 구축 운용되어 왔던 공급사슬관리시스템(SCM)의 핵심적인 성공요인을 도출하였고, 이들 성공요인들 사이의 중요도를 기반으로 한 우선순위를 결정하기 위하여 계층적분석기법(AHP)을 실시하였다. 공급사슬관리시스템의 핵심성공요인의 도출을 위해서는 국내 상장기업 중 공급망관리시스템을 구축 운영하고 있는 기업을 대상으로 인터넷 설문조사를 실시하였고, 수집된 데이터로 실증분석을 하였다. 실증분석 결과 SCM의 주요성공요인은 경영조직요인, 정보기술요인, 외부환경요인 그리고 성과 및 변화관리요인으로 함축되었다. 이들 요인들을 AHP 기법을 통해 우선순위를 분석한 결과로 "IT기술인프라" 요인이 성공요인들 중 우선순위가 가장 높았다. 본 연구의 결과는 한국 제조업들의 공급사슬관리에 있어서 구축 및 관리 방향을 제시해 줄 것으로 판단된다.

• 한국문헌정보학회지
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• 제41권4호
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• pp.273-294
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• 2007

본 연구의 목적은 BSC을 이용한 학술정보 유통기관의 성과지표를 개발하고 전문가를 대상으로 성과지표별 중요도를 분석하는 것이다. 성과지표에 대한 국내외 사례연구를 바탕으로 4개의 관점을 추출한 후 각 관점별로 핵심성공요인과 핵심성과지표를 추출하였다 개발된 성과지표 모형은 정보자원관점, 정보서비스관점, 이용자관점, 경제적관점의 4개 영역으로 구분되고 핵심성공요인은 12항목, 핵심성과지표는 31항목으로 구성하였다. 성과지표별 중요도 분석을 위해 대학, 연구기관 등의 전문가를 대상으로 설문조사를 실시하였으며 분석방법으로 계층화분석법 (AHP)을 사용하였다. 개발된 성과지표의 4가지 관점별 중요도를 분석한 결과 이용자관점, 정보서비스관점, 정보자원관점, 경제적관점 순으로 중요도가 높은 것으로 분석되었다.

• 대한한방소아과학회지
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• 제28권3호
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• pp.31-46
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• 2014

Objectives Forsythiae Fructus treatment has been used for inflammatory and allergic diseases in Korean Medicine. Nevertheless, the mechanism of action and the cellular targets are not understood well. The pathogenesis of allergic diseases are associated with Th2 cytokines such as IL-13, MIP-$1{\alpha}$, IL-13, IL-5, GM-CSF, IL-4, TNF-${\alpha}$ and IL-6, which are secreted by the mast cells. This study was conducted to investigate the effects of Forsythiae Fructus extracts (FF) on Th2 cytokines expression and signal transduction in MC/9 mast cells. Methods In the study, MC/9 mast cells were stimulated with DNP-IgE for 24 hours and then treated separately with CsA $10{\mu}g/m{\ell}$ and varying doses of FF for one hour. MC/9 mast cells stimulated with DNP-IgE was the control group, a treatment with CsA was the positive control group and a treatment with varying doses FF was the experimental groups. The mRNA levels of IL-13, IL-5, GM-CSF, IL-4, TNF-${\alpha}$, IL-6 were analyzed with Real-time PCR. The levels of IL-13, MIP-$1{\alpha}$ were measured using enzyme-linked immunosorbent assays(ELISA). NFAT, AP-1 and NF-${\kappa}B$ p65 were examined by Western blot analysis. Results 1. FF were observed to suppress the mRNA expression of IL-13, IL-5, GM-CSF, IL-4, TNF-${\alpha}$, IL-6 in comparison to DNP-IgE control group. 2. FF also has inhibited the IL-13, MIP-$1{\alpha}$ production significantly in comparison to DNP-IgE control group. 3. Western blot analysis of transduction factors involving Th2 cytokines expression has revealed a prominent decrease of the mast cell specific transduction factors including NFAT-1, NFAT-2, c-Jun, and NF-${\kappa}B$ p65 but c-Fos. Conclusions In conclusion, the anti-allergenic activities of FF may be strongly related to the regulation of transcription factors NFAT-1, NFAT-2, c-Jun, and NF-${\kappa}B$ p65 causing inhibition of Th2 cytokines in mast cells.

• 대한한방소아과학회지
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• 제28권2호
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• pp.23-39
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• 2014

Objectives PRAL (Prunus armniaca Linne Var) is a herbal formula in Oriental Medicine, known for its anti-inflammatory and anti-allergenic properties. However, its mechanism of action and the cellular targets have not yet been found enough. The purpose of this study is to investigate the effects of PRAL on Th2 cytokines expression in MC/9 mast cells. Methods The effect of PRAL was analyzed by ELISA, Real-time PCR, Western blot in MC/9 mast cells. mRNA levels of GM-CSF, IL-4, IL-5, IL-6, IL-13, TNF-${\alpha}$ were analyzed with Real-time PCR. Levels of IL-13, MIP-$1{\alpha}$ were measured using enzyme-linked immunosorbent assays (ELISA). NFAT, AP-1 and NF-${\kappa}B$ p65 were examined by Western blot analysis. Results PRAL inhibited GM-CSF, IL-4, IL-5, IL-6, IL-13, TNF-${\alpha}$ mRNA expression in a dose dependent manner. GM-CSF, IL-4, IL-5 mRNA expression were inhibited significantly in comparison to DNP-IgE control group at concentration of 100 ${\mu}g/ml$ and IL-6, IL-13, TNF-${\alpha}$ mRNA expression were inhibited at concentration of 50 ${\mu}g/ml$, 100 ${\mu}g/ml$. PRAL also inhibited the IL-13, MIP-$1{\alpha}$ production significantly in comparison to DNP-IgE control group in a dose dependent manner. IL-13 production was inhibited at a concentration of 200 ${\mu}g/ml$, 400 ${\mu}g/ml$ and MIP-$1{\alpha}$ was inhibited at a concentration of 100 ${\mu}g/ml$, 200 ${\mu}g/ml$, 400 ${\mu}g/ml$. Western blot analysis of transcription factors involving Th2 cytokines expression revealed prominent decrease of the mast cell specific transcription factors including NFAT-1, c-Jun as well as NF-${\kappa}B$ p65 but not NFAT-2 and c-Fos. Conclusion These results indicate that PRAL has the effect of suppressing Th2 cytokines production in the MC/9 mast cells. These data represent that PRAL potentiates therapeutic activities to the allergic disease by regulating Th2 cytokines in the MC/9 mast cells.

• IMMUNE NETWORK
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• 제5권2호
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• pp.105-109
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• 2005

Background: Dendritic cells (DCs) are the most potent APCs (antigen-presenting cells) and playa critical role in immune responses. Galectin-3 is a biological lectin with a beta-galactoside binding affinity. Recently, proteomic analysis revealed the presence of galectin-3 in the exosome of mature DCs. However, the expression and function of galectin-3 in DCs remains unclear yet. Methods: We used bone marrow-derived DCs of mouse and showed the expression of galectin-3 in DCs by using flow cytometry analysis and Western blot analysis. Results: Galectin-3 was determined as single band of 35 kDa in Western blot analysis. Flow cytometry analysis showed the major growth factor for DCs, granulocyte-macrophage colony stimulating factor (GM-CSF) and maturing agents, anti-CD40 monoclonal antibody (mAb) and lipopolysaccharide (LPS) consistently increased the intracellular expression of galectin-3 in DCs compared to medium alone. In addition, DCs treated with maturing agents did marginally express galectin-3 on their surface. Conclusion: This study suggests that galectin-3 in DCs may be regulated by critical factors for DC function.

• Pediatric Infection and Vaccine
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• 제21권3호
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• pp.219-224
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• 2014

Streptococcus bovis에 의한 신생아 침습감염을 보고한 사례는 많지 않으며, 지금까지 보고된 증례는 대부분 Streptococcus pasteurianus가 원인이었다. 저자들은 생후 28일에 열이 나서 온 환자의 혈액과 뇌척수액에서 분리된 균주를 16S rRNA와 tuf 유전자 염기서열분석을 통해 Streptococcus lutetiensis로 동정할 수 있었다. 환자의 혈액에서 배양된 균주는 자동화 장비(VITEK 2)에서 Streptococcus infantarius로 동정되었고 뇌척수액에서 자란 균주는 동정되지 않았다. 환자는 항균제 투여 2일째부터 열이 떨어지고 전신상태가 호전되었으며, 14일간 항균제 사용 후 신경학적 합병증 없이 퇴원하였다. 저자들은 S. bovis군에 의한 침습 감염 환자에서 정확한 균주 동정을 위해 분자유전학적 검사기법이 도움이 될 수 있음을 확인하였고 본 증례의 원인 균주가 신생아 침습감염의 원인으로 알려진 사례가 없어 보고하는 바이다.

• 한국시스템다이내믹스연구
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• 제2권2호
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• pp.119-134
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• 2001

Since 1998, government-led Korean venture business boom now proceeds into the stage of market-oriented venture business growth. At such a moment, this research is to clarify the relations between the success factors of Korean venture businesses, based on domestic and foreign documentary surveys and inquiry surveys over domestic venture businesses. This research starts from the necessity to overcome the limits of the existing researches by uni-dimensional probing research into success factors of Korean venture businesses and to manifest the multi-dimensional relations between the success factors from the various viewpoints. And this research adopts System-Dynamics methodology to manifest and utilize relations among those factors, avoiding the existing metrical approaches. This research can be called a new approach to the current ecosystems of Korean venture in which whether venture businesses success in Korea is considered to depend on the list on the stock market. For this, this research implemented verification analysis through the simulations of each factor at various levels to build causality map which clarifies the causality of success factors of venture businesses through the System-Dynamics methodology and to utilize it as a way of supporting tool for decision-making of venture businesses. This research will be able to suggest the reactions depending on various internal and external situations. This research tried to manifest the causality map of each factor on the basis of inquiry surveys and documentary surveys to verify feedback among each factor by the SD methodology. This research will be a basis bolster up the still fragile substructure of venture businesses through an efficient analysis framework using the verification of SD methodology and resulting outcomes from this research.

• 한국시스템다이내믹스학회:학술대회논문집
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• 한국시스템다이내믹스학회 2001년도 추계학술대회발표논문집
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• pp.3-14
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• 2001

Since 1998, government-led Korean venture business boom now proceeds into the stage of market-oriented venture business growth. At such a moment, this research is to clarify the relations between the success factors of Korean venture businesses, based on domestic and foreign documentary surveys and inquiry surveys over domestic venture businesses. This research starts from the necessity to overcome the limits of the existing researches by uni-dimensional probing research into success factors of Korean venture businesses and to manifest the multi-dimensional relations between the success factors from the various viewpoints. And this research adopts System-Dynamics methodology to manifest and utilize relations among those factors, avoiding the existing metrical approaches. This research can be called a new approach to the current ecosystems of Korean venture in which whether venture businesses success in Korea is considered to depend on the list on the stock market. For this, this research implemented verification analysis through the simulations of each factor at various levels to build causality map which clarifies the causality of success factors of venture businesses through the System-Dynamics methodology and to utilize it as a way of supporting tool for decision-making of venture businesses. This research will be able to suggest the reactions depending on various internal and external situations. This research tried to manifest the causality map of each factor on the basis of inquiry surveys and documentary surveys to verify feedback among each factor by the SD methodology. This research will be a basis bolster up the still fragile substructure of venture businesses through an efficient analysis framework using the verification of SD methodology and resulting outcomes from this research.

• 경영과학
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• 제13권3호
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• pp.49-73
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• 1996

Common problems have been observed in the existing Information Requirements Analysis (IRA) methodologies such as BSP(IBM), CSF(Rockart), or ISP(Information Engineering), etc. These methodologies require too much time and human resources for the IRA process, involves high complexity, and their IRA outcomes are frequently biased by the subjective views of the people involved. In this paper, we introduce a new methodology for the enterprise wide information requirements analysis utilizing templates and nine closed-ended questions. Extending the BIAIT(Business Information Analysis and Integration Technique) method, this methodology presents nine question about each firm's order type(7Q's for customer side and 2Q's for supplier side) to derive information processing characteristics of the firms. As the central component of this methodology, data and process templates for the manufacturing industry were established. To validate the methodology, suitability of the data and process templates were examined through six real world business cases.