• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1499-1506
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• 2016

Background: Crocus sativus and its major constituent crocin are well established to have anti-cancer properties in breast cancer cells (MCF-7). However the role of C. sativus extract (CSE) and crocin on caspase signaling mediated MCF-7 cell death at molecular level is remains unclear. In this study, we tried to unravel role of CSE and crocin on caspase mediated MCF-7 cells death and their in vivo preclinical toxicity profiling and immune stimulatory effect. Materials and Methods: CSE extract was fractionated by HPLC and crocin was isolated and characterized by NMR, IR, and MS. MCF-7 cells were treated with both CSE and crocin and expression of Bcl-2 and Bax was assessed after 24 and 36 hours. Furthermore, caspase 3, caspase 8 and caspase 9 expression was determined by Western blotting after 24 hours of treatment. DNA fragmentation analysis was performed for genotoxicity of CSE and crocin in MCF-7 cells. The in vivo toxicity profile of CSE (300 mg/kg of b.wt) was investigated in normal Swiss albino mice. In addition, peritoneal macrophages were collected from crocin (1, 1.5 and 2 mg/kg body weight) treated mice and analyzed for ex vivo yeast phagocytosis. Results: Immunoblot analysis revealed that there was time dependent decline in anti-apoptotic Bcl-2 with simultaneous upregulation of Bax in CSE and crocin treated MCF-7 cells. Further CSE and crocin treatment downregulated caspase 8 and 9 and cleaved the caspase 3 after 24 hours. Both CSE and crocin elicited considerable DNA damage in MCF-7 cells at each concentration tested. In vivo toxicity profile by histological studies revealed no observable histopathologic differences in the liver, kidney, spleen, lungs and heart in CSE treated and untreated groups. Crocin treatment elicited significant dose and time dependent ex vivo yeast phagocytosis by peritoneal macrophages. Conclusions: Our study delineated involvement of pro-apoptotic and caspase mediated MCF-7 cell death by CSE and crocin at the molecular level accompanied with extensive DNA damage. Further we found that normal swiss albino mice can tolerate the maximum dose of CSE. Crocin enhanced ex vivo macrophage yeast phagocytic ability.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2007년도 가을 학술발표논문집 Vol.34 No.2 (A)
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• pp.213-214
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• 2007

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2007년도 가을 학술발표논문집 Vol.34 No.2 (A)
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• pp.211-212
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• 2007

• Tuberculosis and Respiratory Diseases
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• 제81권2호
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• pp.138-147
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• 2018

Background: Recent studies show that mitophagy, the autophagy-dependent turnover of mitochondria, mediates pulmonary epithelial cell death in response to cigarette smoke extract (CSE) exposure and contributes to the development of emphysema in vivo during chronic cigarette smoke (CS) exposure, although the underlying mechanisms remain unclear. Methods: In this study, we investigated the role of mitophagy in the regulation of CSE-exposed lung bronchial epithelial cell (Beas-2B) death. We also investigated the role of a phosphodiesterase 4 inhibitor, roflumilast, in CSE-induced mitophagy-dependent cell death. Results: Our results demonstrated that CSE induces mitophagy in Beas-2B cells through mitochondrial dysfunction and increased the expression levels of the mitophagy regulator protein, PTEN-induced putative kinase-1 (PINK1), and the mitochondrial fission protein, dynamin-1-like protein (DRP1). CSE-induced epithelial cell death was significantly increased in Beas-2B cells exposed to CSE but was decreased by small interfering RNA-dependent knockdown of DRP1. Treatment with roflumilast in Beas-2B cells inhibited CSE-induced mitochondrial dysfunction and mitophagy by inhibiting the expression of phospho-DRP1 and -PINK1. Roflumilast protected against cell death and increased cell viability, as determined by the lactate dehydrogenase release test and the MTT assay, respectively, in Beas-2B cells exposed to CSE. Conclusion: These findings suggest that roflumilast plays a protective role in CS-induced mitophagy-dependent cell death.

• 예술인문사회 융합 멀티미디어 논문지
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• 제6권3호
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• pp.41-49
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• 2016

본 논문에서는 현재 전 세계적으로 많은 관심이 집중되어있고, 많은 연구와 개발이 진행되고 있는 사물인터넷 시스템을 개발하였는데, 다양한 표준화 단체들 중에서 가장 많은 연구가 진행된 oneM2M 통신 프로토콜을 사용하였다. oneM2M 통신 프로토콜을 기반으로 하는 본 연구의 사물인터넷 시스템은 크게 ADN-AE 모듈과 CSE 모듈로 나눌 수 있다. AE 모듈은 다양한 서비스의 어플리케이션을 제공하거나 CSE를 관리할 수도 있다. CSE 모듈은 사물인터넷의 다양한 AE들에게 공통적으로 제공해야하는 서비스 기능들로 이루어진 플랫폼이다. CSE 모듈은 Network Manager, Message Handler, Resource Manager 모듈 등으로 구성된다. Network Manager 모듈은 oneM2M 기반 통신의 전반적인 관리와 시스템 전체의 흐름을 관장한다. Message Handler 모듈은 송수신한 모든 메시지들의 관리 및 분석을 담당하고, Resource Manager 모듈은 리소스 트리에 관한 모든 관리를 담당하도록 하였다. 리소스 트리는 관리되어야 할 오브젝트에 관한 정보를 저장한다. 각 모듈에서의 자료 흐름 및 프로토콜 매핑 등에 관해서도 설명하였다.

• 여성건강간호학회지
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• 제22권3호
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• pp.151-161
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• 2016

Purpose: The purpose of this study was to develop and test the validity and reliability of the Korean version of the Contraceptive Self-efficacy (K-CSE) scale. Methods: The K-CSE scale was developed through forward-backward translation methods. Construct and criterion validity and internal consistency reliability were calculated using SPSS/WIN 21.0. Data were collected from 257 women students from two universities in G city, South Korea. Results: The factor structure of the K-CSE scale showed the cumulative variance as 62.9% in the factor analysis. Factor loading of 13 items on four subscales ranged from .47 to .88. Factors were named as 'initiative of contraceptive use', 'sexual assertiveness', 'refusal of sexual intercourse', and 'autonomic sexual behavior'. Criterion validity compared to the Sexual Autonomy Measurement (SAM) showed significant correlation. Cronbach's ${\alpha}$ for the reliability of each subscale were .50~.82 and .76 for the total scale. Conclusion: The findings of this study demonstrate that the K-CSE scale is a satisfactory valid and reliable scale. Therefore, the CSE scale can be useful in measuring contraceptive self-efficacy among women university students in Korea.

• 대한물리의학회지
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• 제17권4호
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• pp.1-13
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• 2022

PURPOSE: This study examined the effects of the craniovertebral angle, proprioception (joint error test), and the upper trapezius on the muscle tone when comparing cervical stabilization and scapula stabilization exercises and when two exercises were performed together. METHODS: The participants in this study agreed in advance, and this study was carried out by recruiting 27 university students in their twenties with mild frontal posture. The subjects were assigned randomly to three groups that performed cervical stabilization exercises, scapular stabilization exercises, and both cervical and scapular stabilization exercises. One-way repeated ANOVA was used to analyze the evaluation values of the 1^st, 3^rd, and 6^th weeks of exercise intervention within the group, and one-way ANOVA was used to compare the difference in the effects of exercise intervention among the three groups. RESULTS: Proprioception was significantly different in the cervical stabilization exercises (CSE) group and the cervical stabilization exercises + Scapular stabilization exercises (CSE+SSE) groups at three weeks, and there was a significant difference between the scapular stabilization exercises (SSE) group and the CSE+SSE group (p < .05). At six weeks, there was a significant difference between the CSE group and the CSE+SSE group, and there was a significant difference between the SSE group and the CSE+SSE group (p < .05). There was a significant difference between three and six weeks in the CSE group (p < .05). In the SSE group, there was a significant difference between pre and six weeks, and between three and six weeks (p < .05). In the CSE+SSE group, there was a significant difference between pre and three weeks, and between pre and six weeks (p < .05). On the other hand, there were no significant differences between CVA and muscular tone in all three groups (P > .05). CONCLUSION: In all groups, the proprioception (joint error test) showed significant improvement, and the CSE+SSE group showed greater improvement than the other groups. As a result, the appropriate combination of neck stabilization exercise and scapular stabilization exercise effectively improved proprioception in the presence of forward head posture (FHP).

• 한국식품과학회지
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• 제52권2호
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• pp.144-148
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• 2020

Corn silk (Okmi-su) was anciently adopted as a material for tea or beverage. Corn silk extracts (CSE) contain bioactive phytochemicals such as phenolic acid, flavonoids, ascorbic acid, tannins, and glycosides. Under the impact of these functional components, CSE has benefits for antioxidation, diuresis, anti-diabetes, and dyslipidemia recovery. Nonetheless, its role in whole-body adiposity was not investigated; therefore, the effects of CSE on obesity were evaluated in high-fat diet-induced obese mice. Mice were assigned to either group (n=12); 1) normal diet (18% kcal from fat), 2) high-fat diet (45% kcal from fat, the control), 3) high-fat diet with CSE (800 mg/kg diet), and 4) high-fat diet with orlistat (500 mg/kg diet, a comparable control for weight loss). Our results showed that body weight, adiposity, and energy expenditure in obese mice were not altered by CSE. Lean body mass tended to decrease by CSE, which can be explained by stimulation of diuresis (p=0.06). In conclusion, our results suggest that dietary consumption of CSE does not influence the adiposity and underlying substrate utilization in high-fat diet-induced obese mice.

• Nutrition Research and Practice
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• 제11권5호
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• pp.373-380
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• 2017

BACKGROUND/OBJECTIVES: This study was conducted to investigate the effect of a corn silk extract on improving benign prostatic hyperplasia (BPH). MATERIALS/METHODS: The experimental animals, 6-week-old male Wistar rats, were divided into sham-operated control (Sham) and experimental groups. The experimental group, which underwent orchiectomy and received subcutaneous injection of 10 mg/kg of testosterone propionate to induce BPH, was divided into a Testo Only group that received only testosterone, a Testo+Fina group that received testosterone and 5 mg/kg finasteride, a Testo+CSE10 group that received testosterone and 10 mg/kg of corn silk extract, and a Testo+CSE100 group that received testosterone and 100 mg/kg of corn silk extract. Prostate weight and concentrations of dihydrotestosterone (DHT), $5{\alpha}$- reductase $2(5{\alpha}-R2)$, and prostate specific antigen (PSA) in serum or prostate tissue were determined. The mRNA expressions of $(5{\alpha}-R2)$ and proliferating cell nuclear antigen (PCNA) in prostate tissue were also measured. RESULTS: Compared to the Sham group, prostate weight was significantly higher in the Testo Only group and decreased significantly in the Testo+Fina, Testo+CSE10, and Testo+CSE100 groups (P < 0.05), results that were consistent with those for serum DHT concentrations. The concentrations of $(5{\alpha}-R2)$ in serum and prostate as well as the mRNA expression of $(5{\alpha}-R2)$ in prostate were significantly lower in the Testo+Fina, Testo+CSE10, and Testo+CSE100 groups than that in the Testo Only group (P < 0.05). Similarly, the concentrations of PSA in serum and prostate were significantly lower in the Testo+Fina, Testo+CSE10, and Testo+CSE100 groups (P < 0.05) than in the Testo Only group. The mRNA expression of PCNA in prostate dose-independently decreased in the Testo+CSE-treated groups (P < 0.05). CONCLUSIONS: BPH was induced through injection of testosterone, and corn silk extract treatment improved BPH symptoms by inhibiting the mRNA expression of $(5{\alpha}-R2)$ and decreasing the amount of $(5{\alpha}-R2)$, DHT, and PSA in serum and prostate tissue.

• 한국식품영양과학회지
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• 제38권12호
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• pp.1672-1678
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• 2009

유자씨 종피와 배유부분에 대한 n-hexane 및 ethanol 추출물에 대한 항산화활성, ACE 저해활성, $\alpha$-glucosidase 억제활성 및 항암활성을 살펴보았다. 항산화활성은 종피 nhexane 추출물(CSH1), 배유 n-hexane 추출물(CSH2), 종피 70% ethanol 추출물(CSE1) 및 배유 70% ethanol 추출물(CSE2) 가운데 CSE1이 가장 높은 활성을 나타내었으며, CSE1의 용매분획물 가운데 ethylacetate 층이 2 mg/mL의 농도에서 라디칼소거능이 73.81% 그리고 총항산화력이 56.64 mg AA eq/g으로 우수한 항산화활성을 나타내었다. 또한 ACE 저해활성과 $\alpha$-glucosidase 억제활성도 CSE1이 각각 31.63% 및 45.17%로 가장 높은 활성을 나타내었다. 각각의 추출물에 대한 유방암, 간암, 폐암, 대장암 및 전립선암 세포에 대한 증식억제효과는 모든 암세포에 대하여 5mg/mL의 농도에서 종피 ethanol 추출물(CSE1)이 가장 높은 암세포 증식억제효과를 보였다.