• Journal of Acupuncture Research
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• v.20 no.6
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• pp.89-102
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• 2003

Objective: The expression of CRF(corticotropin releasing factor), CRF-R(receptor) and CRF-BP(binding protein) in CNS neurons projecting to the stomach and ST36 using the pseudorabies virus in the rat was investigated. Methods: After survival times of 5 days following injection of PRV-Ba-Gal, The thirty rats were perfused, and their brain were frozen sectioned($30{\mu}m$). These sections were stained by PRV-Ba-Gal histochemical staining method and(or) CRF, CRF-R and CRF-BP immunohistochemical method. The common expressed areas of the brain projecting to the stomach and zusanli(ST36) following injection of PRV-Ba-Gal were observed with light microscope. Results: 1) The dense accumulation of CRF-immunoreactive terminals is seen in the area postrema, n. tractus solitarius, external zone of the median eminence, with some immunoreactive CRF also present in the internal zone. 2) Aggregates of CRF-R immunoreactive perikarya are found in area postrema, n. tractus solitarius, lateral reticular n., gigantocellular reticular n., locus coeruleues, paraventricular n. of hypothalamus, median eminence, preoptic n., arcuate n. and hind limb area of cerebral cortex. 3) Aggregates of CRF-BP immunoreactive perikarya are found in area postrema, n. tractus solitarius, lateral reticular nucleus, gigantocellular reticular n., locus coeruleues, paraventricular n. of hypothalamus, median eminence and arcuate n.. Conclusions : These results suggest that PRV-Ba-Gal labeled areas projecting to stomach and ST36 may be related to the central autonomic pathways. A part of CNS neurons projecting to the stomach and ST36 were related to expression of CRF, CRF-R and CRF-BP related to the stress in central autonomic center.

• Annual Conference on Human and Language Technology
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• 2013.10a
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• pp.9-12
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• 2013

본 논문에서는 한국어 형태소 분할 및 품사 태깅 방법을 위한 결합 모델로 Semi-CRF와 Linear-chain CRF에 대한 초기 비교 실험을 수행한다. Linear-chain방법은 출력 레이블을 형태소 분할 정보와 품사 태그를 조합함으로써 결합을 시도하는 방식이고, Semi-CRF는 출력의 구조가 분할과 태깅 정보를 동시에 포함하도록 표현함으로써, 디코딩 과정에서 분할과 태깅을 동시에 수행하는 방법이다. Sejong품사 부착말뭉치에서 비교결과 Linear-chain방법이 Semi-CRF방법보다 우수한 성능을 보여주었다.

• Annual Conference on Human and Language Technology
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• 2015.10a
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• pp.224-227
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• 2015

상호참조해결은 특정 개체에 대해 다르게 표현한 단어들을 서로 연관지어 주며, 이러한 개체에 대해 표현한 단어들을 멘션(mention)이라 하며, 이런 멘션을 찾아내는 것을 멘션탐지(mention detection)라 한다. 멘션은 명사나 명사구를 기반으로 정의되며, 명사구의 경우에는 수식어를 포함하기 때문에 멘션탐지를 순차 데이터 문제(sequence labeling problem)로 정의할 수 있다. 순차 데이터 문제에는 Recurrent Neural Network(RNN) 종류의 모델을 적용할 수 있으며, 모델들은 Long Short-Term Memory(LSTM) RNN, LSTM Recurrent CRF(LSTM-CRF), Bidirectional LSTM-CRF(Bi-LSTM-CRF) 등이 있다. LSTM-RNN은 기존 RNN의 그레디언트 소멸 문제(vanishing gradient problem)를 해결하였으며, LSTM-CRF는 출력 결과에 의존성을 부여하여 순차 데이터 문제에 더욱 최적화 하였다. Bi-LSTM-CRF는 과거입력자질과 미래입력자질을 함께 학습하는 방법으로 최근에 가장 좋은 성능을 보이고 있다. 이에 따라, 본 논문에서는 멘션탐지에 Bi-LSTM-CRF를 적용할 것을 제안하며, 각 딥 러닝 모델들에 대한 비교실험을 보인다.

• The Journal of the Korea Contents Association
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• v.12 no.12
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• pp.714-720
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• 2012

With the help of information technology, it is the most important to collect clinical data correctly in clinical trials. For this, recently, in many pharmaceutical companies and Clinical Research Organizations (CRO) have tried to adopt an Electronic Case Report Form (eCRF) system. The eCRF system is a computerized system designed for collecting and managing clinical data in clinical trials. Although the eCRF is recognized as a significant system for clinical trials, until now there are no widely useable, due to the huge effort and expense at the initiatory stage of adopting eCRF system. In this study, we inquired the use of eCRF system developed for clinical trial at Korea Institute of Oriental Medicine. Especially, we focused on the time required for entering clinical data into the eCRF, hours of use, and the number of queries according to the time flow.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.40 no.4
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• pp.413-421
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• 2014

Corticotropin-releasing factor (CRF) is involved in the stress response and there is increasing evidence that stress influences skin disease such as hair loss. In cultured human hair follicles, CRF inhibits hair shaft elongation, induces premature regression and promotes the apoptosis of hair matrix keratinocytes. We investigated whether CRF influences the dermal papilla cells (DPC) that play pivotal roles in hair growth and cycling. Human DPCs were treated with CRF, adrenocorticotropic hormone (ACTH) and cortisol, key stress hormones along the hypothalamic-pituitary -adrenal (HPA) axis for 1-24 h. Interestingly, CRF modulated the expression of cytokines related to hair growth (KGF, Wnt5a, $TGF{\beta}-2$, Nexin) and increased cAMP production in cultured DPCs. CRF receptors were down-regulated by negative feedback systems. Pretreatment of CRF receptor antagonists or protein kinase A (PKA) inhibitor prevented the CRF-induced modulation. Since the CRF induces proopiomelanocortin (POMC) expression through the cAMP/PKA pathway, we analyzed POMC mRNA. CRF stimulated POMC expression in cultured human DPCs, yet we were unable to detect ACTH levels by western blot. These results indicate that CRF operates within DPCs through CRF receptors along the classical CRF signaling pathway and CRF receptor antagonists could serve as potential therapeutic and cosmetic agents for stress-induced hair loss.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.11
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• pp.7575-7581
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• 2015

Corticotropin-releasing factor(CRF), one of the stress driven neuropeptides, was widely proposed to influence hair loss and re-growth. For the development of receptor antagonists, the screening system based on intracellular calcium signal process was developed and optimized. The aequorin parental cells were transfected with CRF1 receptor and alpha 16 promiscuous G protein cDNA to establish HEK293a16/hCRF1, a stable cell line for the human CRF1 receptor. In HEK293a16/hCRF1 cells, the range of sauvagine dose response was 12-fold higher($EC_{50}:15.21{\pm}1.83nM$) than in the transiently expressed cells, hence essential conditions for the antagonist screening experiments such as the robust signals and high solvent tolerance were secured. The standard antagonists for the CRF1 receptor, antalarmin and CP154526, resulted $IC_{50}$ values of $414.1{\pm}5.5$ and $290.7{\pm}1.9nM$, respectively. Similar results were presented with frozen HEK293a16/hCRF1 cells. Finally, our HEK293a16/hCRF1 cells with the aequorin based cellular functional assay can be a model system for the development of functional cosmetics and modulators that can have a clinical efficacy on hair re-growth.

• Animal Bioscience
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• v.35 no.12
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• pp.1904-1910
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• 2022

Objective: This study aimed to investigate the effects of corticotropin-releasing factor (CRF) on the feed intake of broiler chickens and explore its influencing mechanism. Methods: The study included two trials. In trial 1, 32 male broiler chickens (Arbor Acres, Gallus gallus domesticus) were given ventricle buried tubes, and they were allowed to recover for 3 days. At 8:00 AM, intracerebroventricular (ICV) injection with CRF or normal saline was performed in 10-day-old broiler chickens, which were divided into the 5, 10, and 20 ㎍ and control (normal saline) groups according to the dose of CRF injection. In trial 2, chickens were divided into the 10 ㎍ and control group (physiological saline) to repeat trial 1. Results: Results of trial 1 showed that the cumulative amount of feed intake in the 10 or 20 ㎍ groups was considerably lower than that of the control group after ICV injection with CRF. The lowest amount of feed intake was obtained with the addition of 10 ㎍ of CRF. In trial 2, the expression of ghrelin in the hypothalamus injected with 10 ㎍ of CRF increased significantly, but the expression of ghrelin in various sections of the small intestine considerably decreased. The expression of CRF receptor subtypes 1 (CRFR1) in the hypothalamus and some parts of the small intestine remarkably increased, and the expression of CRF receptor subtypes 2 (CRFR2) increased only in the duodenum, whereas the expression of growth hormone secretagogue receptor (GHSR-1α) in the jejunum and ileum increased considerably after ICV injection of 10 ㎍ of CRF. Conclusion: The CRF at 10 ㎍ increased ghrelin expression in the hypothalamus and CRFR1 expression in the small intestine, and this phenomenon was related to the suppressed feed intake of broiler chickens.

• Proceedings of the KAIS Fall Conference
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• 2010.11b
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• pp.673-676
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• 2010

본 연구에서는 임상시험 데이터를 수집하는 CRF를 웹의 표준인 XML 기반으로 설계하고, 임상시험데이터 표준인 SDTM으로 변환된 eCRF를 생성하는 XML 기반 프로세스을 개발했다. 데이터구조와 폼이 분리되는 XML 기반 개발 프로세스는 XML Schema로 eCRF를 개발하고, SDTM을 적용한 XML 문서를 생성한다. 생성된 XML 문서에서 SDTM 규약에 맞는 도메인별로 변환방법을 제시한다. 이에 eCRF 분석과 데이터베이스 설계 및 구현이 동시에 이루어진다.

• Korean Journal of Clinical Pharmacy
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• v.16 no.1
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• pp.40-45
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• 2006

In clinical trials, it is important to have well designed case report forms (CRF) in order to obtain good quality of data. If CRF design at the first stage of your study goes wrong, after all efforts have been made, you may find practical difficulties in data analysis at the final stage of your study because of incomplete or wrong information. In this paper, the following rules fundamental to CRF design are introduced: rules in general, rules of format or style, categorized responses, forms with multiple records per subject, and international studies. Investigators are well aware of research goals, however sometimes they do not know how to express it on CRF. If they understand the rules fundamental to CRF design, time for CRF development will be saved and CRF completeness will be enhanced.

• 한국어정보학회:학술대회논문집
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• 2017.10a
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• pp.338-339
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• 2017

본 논문은 개체명 인식을 위해 CRF 모델을 이용해 분류를 수행했다. 개체명 후보를 개체명으로 식별에서 중의성 문제가 필요하다. 본 논문에서는 이러한 중의성 문제 해결을 위해 학습 셋으로부터 패턴과 형태적 특성을 고려해 개체명 후보를 최대로 선택하고 선택된 개체명 후보의 중의성과 정확도를 높이기 위해 주변의 문맥 자질과 분별 확률 모델인 CRF를 이용해 중의성 문제를 해결한다.