• International Journal of Oral Biology
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• v.35 no.4
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• pp.209-214
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• 2010

Cells that have endogenous multipotent properties can be used as a starting source for the generation of induced pluripotent cells (iPSC). In addition, small molecules associated with epigenetic reprogramming are also widely used to enhance the multi- or pluripotency of such cells. Skinderived precursor cells (SKPs) are multipotent, sphereforming and embryonic neural crest-related precursor cells. These cells can be isolated from a juvenile or adult mammalian dermis. SKPs are also an efficient starting cell source for reprogramming and the generation of iPSCs because of the high expression levels of Sox2 and Klf4 in these cells as well as their endogenous multipotency. In this study, valproic acid (VPA), a histone deacetylase (HDAC) inhibitor, was tested in the generation of iPSCs as a potential enhancer of the reprogramming potential of SKPs. SKPs were isolated from the back skins of 5-6 week old C57BL/6 X DBA/2 F1 mice. After passage 3, the SKPs was treated with 2 mM of VPA and the quantitative real time RT-PCR was performed to quantify the expression of Oct4 and Klf4 (pluripotency specific genes), and Snai2 and Ngfr (neural crest specific genes). The results show that Oct4 and Klf4 expression was decreased by VPA treatment. However, there were no significant changes in neural crest specific gene expression following VPA treatment. Hence, although VPA is one of the most potent of the HDAC inhibitors, it does not enhance the reprogramming of multipotent skin precursor cells in mice.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.43 no.2
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• pp.100-105
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• 2017

Objectives: Localization of the mandibular canal (MC) and measurement of the height and width of the available alveolar bone at the proposed implant site in the posterior segment of the mandible using cone-beam computed tomography (CBCT) in patients with a single missing tooth. Materials and Methods: A cross-sectional study was performed where CBCT scans of the patients with a single missing tooth in the posterior segment of the mandible-premolar, I (1st) molar, and II (2nd) molar were used. The scans were assessed using OnDemand3D software (version 1.0; CyberMed Inc., Seoul, Korea) for localization of the MC asnd remaining alveolar bone both vertically (from the superior position of the MC to the crest of the alveolar ridge) and horizontally (buccolingual, 3 mm below the crest of the alveolar ridge). The findings were statistically analyzed using independent t-test. Results: A total of 120 mandibular sites (40 sites for each of the three missing premolar, I molar, and II molar) from 91 CBCT scans were analyzed. The average heights (from the alveolar crest to the superior margin of the MC) at the premolar, I molar, and II molar areas were $15.19{\pm}2.12mm$, $14.53{\pm}2.34mm$, and $14.21{\pm}2.23mm$, respectively. The average widths, measured 3 mm below the crest of the alveolar ridge, at the premolar, I molar, and II molar areas were $6.22{\pm}1.96mm$, $6.51{\pm}1.75mm$, and $7.60{\pm}2.08mm$, respectively. There was no statistically significant difference between males and females regarding the vertical and horizontal measurements of the alveolar ridges. Conclusion: In the study, the measurements were averaged separately for each of the single missing teeth (premolar, I molar, or II molar), giving more accurate information for dental implant placement.

• Journal of Periodontal and Implant Science
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• v.31 no.2
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• pp.477-488
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• 2001

The present study evaluated of regeneration effect of platelet rich plasma on the treatment of grade II furcation involvement, with coralline calcium phosphate bone in humans. 30 teeth with grade II furcation defect were selected and 15 teeth(control) were treated with coralline calcium phosphate bone, the others(test) were treated with coralline calcium phosphate bone and platelet rich plasma. Pocket depth, clinical attachment level, width of keratinized gingiva width were measured at baseline, postoperative 3, 6months. from cementoenamel junction to alveolar crest and fundus were measured at baseline, 6months(re-entry). Both groups were statistically analyzed by Wilcoxon signed Ranks Test & Mann-whitney Test using SPSS program(5% significance level). The results were as follows: 1. Pocket depth, clinical attachment level, keratinized gingva width, cementoenamel junction - alveolar crest, cementoenamel junction - fundus were not differ significantly in both groups at baseline 2. The change of pocket depth, clinical attachment level, keratinized gingva width, cementoenamel junction - alveolar crest, cementoenamel junction - fundus in both groups were decreased significantly at 3, 6months(p<0.05). 3. The change of pocket depth, clinical attachment level in test group decreased significantly than control at 3, 6months(p<0.05). 4. The change of keratinized gingiva width, cementoenamel junctional - alveolar crest, cementoenamel junction - fundus were not differ significantly in both groups at 3, 6months. 5. The pocket depth, clinical attachment level, keratinized gingiva width exhibited marked changes at 3 months in both groups. In conclusion, the results of this study suggest that platelet rich plasma have adjunctive clinical treatment effect to periodontal regeneration in grade II furcation defects.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.22 no.6
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• pp.397-404
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• 2010

Laboratory tests of wave overtopping rates for a rubble mound breakwaters armoured Tetrapod were carried out, with varying design waves, crest berm widths and crest freeboards. The objective of this study is to investigate overtopping performance and to examine the characteristics of the widely used overtopping prediction models through the results of laboratory tests. Laboratory tests show that structure slope and wave periods have a considerable influence on overtopping rates, but the difference of overtopping rates related to crest berm widths is slight. Owen(1980)'s prediction considerably overestimates compared to the measured valued. Prediction of Van der Meer et al.(1998) underestimates only for steep slope($cot{\alpha}$=1.5). Besley(1999)'s and Pedersen(1996)'s predictions have a relatively good agreement with the measured results for slopes with a broader crest berm width. In general, best agreement between measured and predicted overtopping rates is observed using modified Pedersen(1996)'s formula for all test conditions.

• Journal of The Korean Society of Agricultural Engineers
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• v.61 no.2
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• pp.13-23
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• 2019

In this study, an overtopping model experiments and three dimensional seepage characteristics at the deteriorated homogeneous reservoirs were performed to investigate the behavior of failure for embankment and spillway transitional zone due to overtopping. The failure pattern, pore water pressure, earth pressure and settlement by overtopping were compared and analyzed. The pattern of the failure by overtopping was gradually enlarged towards reservoirs crest from the spillway transition zone at initial stage. In the rapid stage and peak stage, the width and depth of failure gradually increased, and the pattern of the failure appeared irregular and several direction of the erosion. In the early stage, the pore water pressure at spillway transitional zone was more affected as its variation and failure width increased. In the peak stage, the pore water pressure was significantly increased in all locations due to the influence of seepage. The earth pressure increased gradually according to overtopping stage. The pore pressure by the numerical analysis was larger than the experimental value, and the analysis was more likely to increase steadily without any apparent variation. The horizontal and vertical displacements were the largest at the toe of slope and at the top of the dam crest, respectively. The results of this displacement distribution can be applied as a basis for determining the position of reinforcement at the downstream slope and the crest. The collapse in the overtopping stage began with erosion of the most vulnerable parts of the dam crest, and the embankment was completely collapsed as the overtopping stage increased.

• Journal of Korean Neurosurgical Society
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• v.66 no.6
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• pp.703-715
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• 2023

Objective : This retrospective study investigated the factors that affect cage obliquity angle despite orthogonal maneuvers performed during oblique lateral interbody fusion (OLIF) and assessed the relationship between cage obliquity angle and radiological outcomes post-surgery. Methods : Twenty-nine males who underwent L4-L5 OLIF for lumbar degenerative disease between 2019 and 2021 with a followup duration greater than 12 months were analyzed. Radiological parameters were measured including psoas muscle volume, total psoas area index (total psoas muscle area [cm²]/height squared [m²]), distance from the iliac artery to the origin of the psoas muscle (DIAPM), angle between the origin of the psoas muscle and the center of the vertebral disc (APCVD), iliac crest height, disc height, lumbar flexibility (lumbar flexion angle minus extension angle), cage location ratio, cage-induced segmental lumbar lordosis (LL) (postoperative index level segmental LL minus used cage angle), foraminal height changes, fusion grade. Results : DIAPM, APCVD, iliac crest height, postoperative index level segmental LL, and cage-induced segmental LL were significantly correlated with OLIF cage obliquity angle. However, other radiological parameters did not correlate with cage obliquity. Based on multiple regression analysis, the predictive equation for the OLIF cage obliquity angle was 13.062-0.318×DIAPM+0.325×1APCVD+0.174×iliac crest height. The greater the cage obliquity, the smaller the segmental LL compared to the cage angle used. Conclusion : At the L4-L5 level, OLIF cage obliquity was affected by DIAPM, APCVD, and iliac crest height, and as the cage obliquity angle increases, LL agnle achievable by the used cage could not be obtained.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.21 no.2
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• pp.377-392
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• 1991

The aim of this study was to investigate the thickness of alveolar lamina dura and alveolar bone height by the change of periodontal status. The periapical radiograms of the 853 cases were taken by paralleling technique. The conclusions from this study were as follows: 1. In the normal periodontal status, the alveolar lamina dura was the thickest at alveolar crest, followed by apex, midroot, and the average thickness of the posterior teeth was thicker than that of the anterior teeth. 2. In Russel's Periodontal Index 0 and 1, no significant differences of thickness were found to be 3 areas. In Russel's Periodontal Index 2 and 6, the thickness of alveolar lamina dura at alveolar crest was decreased, only. 3. In Russel's Periodontal Index 0 and 1, distance from the cemento-enamel junction to the alveolar crest was within 1.5㎜. In Russel's Periodontal Index 2 and 6, the distance was 3. increased over 1.5㎜.

• IMMUNE NETWORK
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• v.8 no.1
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• pp.1-6
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• 2008

Apoptosis signal-regulating kinase 1 (ASK1), a mitogen- activated protein kinase kinase kinase, plays pivotal roles in stress responses. In addition, ASK1 has emerged as a key regulator of immune responses elicited by pathogen-associated molecular patterns (PAMPs) and endogenous danger signals. Recent studies have demonstrated that reactive oxygen species (ROS)-dependent activation of ASK1 is required for LPS-stimulated cytokine production as well as extracellular ATP-induced apoptosis in immune cells. The mechanism of ROS-dependent regulation of ASK1 activity by thioredoxin and TRAFs has been well characterized. In this review, we focus on the molecular details of the activation of ASK1 and its involvement in innate immunity.

• Journal of Korea Water Resources Association
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• v.36 no.3 s.134
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• pp.437-445
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• 2003

Since many islands are located in the South Sea, unexpected damage by tsunamis could be caused by mutual interferences between adjacent islands. In this study, the variation of run-up heights is investigated by using different crest lengths of incident waves and different distances between two adjacent islands. The run-up height sharply increases when the crest length of Incident waves is greater than the distance bewteen outer boundaries of two islands. The run-up height also increases as the distance between two adjacent islands decreases.

• International Journal of Oral Biology
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• v.37 no.4
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• pp.175-180
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• 2012

Skin-derived precursor cells (SKPs) are multipotent, sphere-forming and embryonic neural crest-related precursor cells that can be isolated from dermis. It is known that the properties of porcine SKPs can be enhanced by leukemia inhibitory factor (LIF) which is an essential factor for the generation of embryonic stem cells in mice. In our present study, to enhance or maintain the properties of murine SKPs, LIF was added to the culture medium. SKPs were treated with 1,000 IU LIF for 72 hours after passage 3. Quantitative real time RT-PCR was then performed to quantify the expression of the pluripotent stem cell specific genes Oct4, Nanog, Klf4 and c-Myc, and the neural crest specific genes Snai2 and Ngfr. The results show that the expression of Oct4 is increased in murine SKPs by LIF treatment whereas the level of Ngfr is decreased under these conditions. Interestingly, LIF treatment reduced Nanog expression which is also important for cell proliferation in adult stem cells and for osteogenic induction in mesenchymal stem cells. These findings implicate LIF in the maintenance of stemness in SKPs through the suppression of lineage differentiation and in part through the control of cell proliferation.