• 한국지반공학회논문집
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• 제36권9호
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• pp.5-20
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• 2020

2017년 발생한 포항지진에서는 계기지진 이래 처음으로 액상화 현상이 목격되었다. 본 연구에서는 액상화 현상이 목격된 포항시 (1) 송림공원, (2) 흥해읍 망천리, (3) 흥안리를 대상으로 다양한 지반조사 및 실내실험을 수행하였다. 액상화 발생위치의 동적특성을 파악하기 위해 지반조사 항목으로는 표준관입시험(SPT), 콘관입시험(CPT), 표면파탐사(MASW), 밀도검층, 다운홀테스트, 전기비저항탐사를 수행하였고, 현장에서 채취한 시료를 바탕으로 진동삼축압축 시험기를 통해 액상화실험을 수행하였다. 그 결과 세 곳 모두 지하수위가 높고 얕은 심도에 연약한 모래층이 존재하며, 송림공원에서 채취한 모래는 주문진 표준사, Toyoura 모래, Ottawa 모래에 비해 액상화 저항강도가 작은 것을 확인하였다.

• The Korean Journal of Physiology and Pharmacology
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• 제2권6호
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• pp.733-742
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• 1998

Background: We have previously reported that not only cGMP but also 8-Br-cGMP or 8-pCPT-cGMP, specific and potent stimulators of cGMP-dependent protein kinase (cGMP-PK), increased basal L-type calcium current $(I_{Ca})$ in rabbit ventricular myocytes. Our findings in rabbit ventricular myocytes were entirely different from the earlier findings in different species, suggesting that the activation of cGMP-PK is involved in the facilitation of $I_{Ca}}$ by cGMP. However, there is no direct evidence that cGMP-PK can stimulate $I_{Ca}}$ in rabbit ventricular myocytes. In this report, we focused on the direct effect of cGMP-PK on $I_{Ca}}$ in rabbit ventricular myocytes. Methods and Results: We isolated single ventricular myocytes of rabbit hearts by using enzymatic dissociation. Regulation of $I_{Ca}}$ by cGMP-PK was investigated in rabbit ventricular myocytes using whole-cell voltage clamp method. $I_{Ca}}$ was elicited by a depolarizing pulse to +10 mV from a holding potential of -40 mV. Extracellular 8-(4-Chlorophenylthio)-guanosine-3',5'-cyclic monophosphate (8-pCPT-cGMP), potent stimulator of cGMP-dependent protein kinase (cGMP-PK), increased basal $I_{Ca}}$. cGMP-PK also increased basal $I_{Ca}}$. The stimulation of basal $I_{Ca}}$ by cGMP-PK required both 8-Br-cGMP in low concentration and intracellular ATP to be present. The stimulation of basal $I_{Ca}}$ by cGMP-PK was blocked by heat inactivation of the cGMP-PK and by bath application of 8-(4-chlorophenylthio)-guanosine-3',5'-cyclic monophosphate, Rp-isomer (Rp-pCPT-cGMP), a phosphodiesterase-resistant cGMP-PK inhibitor. When $I_{Ca}}$ was increased by internal application of cGMP-PK, IBMX resulted in an additional stimulation of $I_{Ca}}$. In the presence of cGMP-PK, already increased $I_{Ca}}$ was potentiated by bath application of isoprenaline or forskolin or intracellular application of cAMP. Conclusions: We present evidence that cGMP-PK stimulated basal $I_{Ca}}$ by a direct phosphorylation of L-type calcium channel or associated regulatory protein in rabbit ventricular myocytes.

• Asian-Australasian Journal of Animal Sciences
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• 제28권3호
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• pp.411-419
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• 2015

We previously demonstrated that bovine subcutaneous preadipocytes promote adipogenic gene expression in muscle satellite cells in a co-culture system. Herein we hypothesize that saturated fatty acids would promote adipogenic/lipogenic gene expression, whereas mono- and polyunsaturated fatty acids would have the opposite effect. Bovine semimembranosus satellite cells (BSC) and intramuscular preadipocytes (IPA) were isolated from crossbred steers and cultured with 10% fetal bovine serum (FBS)/Dulbecco's Modified Eagle Medium (DMEM) and 1% antibiotics during the 3-d proliferation period. After proliferation, cells were treated for 3 d with 3% horse serum/DMEM (BSC) or 5% FBS/DMEM (IPA) with antibiotics. Media also contained $10{\mu}g/mL$ insulin and $10{\mu}g/mL$ pioglitazone. Subsequently, differentiating BSC and IPA were cultured in their respective media with $40{\mu}M$ palmitic, stearic, oleic, or linoleic acid for 4 d. Finally, BSC and IPA were single- or co-cultured for an additional 2 h. All fatty acid treatments increased (p = 0.001) carnitine palmitoyltransferase-1 beta ($CPT1{\beta}$) gene expression, but the increase in $CPT1{\beta}$ gene expression was especially pronounced in IPA incubated with palmitic and stearic acid (6- to 17-fold increases). Oleic and linoleic acid decreased (p = 0.001) stearoyl-CoA desaturase (SCD) gene expression over 80% in both BSC and IPA. Conversely, palmitic and stearic acid increased SCD gene expression three fold in co-cultured in IPA, and stearic acid increased $AMPK{\alpha}$ gene expression in single- and co-cultured BSC and IPA. Consistent with our hypothesis, saturated fatty acids, especially stearic acid, promoted adipogenic and lipogenic gene expression, whereas unsaturated fatty acids decreased expression of those genes associated with fatty acid metabolism.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제7권2호
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• pp.190-202
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• 1996

본 논문은 주의산만이나 과잉운동을 주소로 대학병원 정신과에 내원한 5세에서 13세 사이의 환아들을 대상으로 인구학적 특성과 주진단 및 공존질병에 대해서 조사하였고, 그 중에서 주의력결핍 과잉운동장애(ADHD)로 진단 받은 환아들의 인구학적 및 임상적 특성을 조사하였으며, 공존질병이 있는 집단과 ADHD만 있는 집단을 비교 분석하였다. 또한 ADHD군과 외향적장애군 및 내향적장애군 등 세집단간의 임상특성과 심리검사상의 차이를 알아보았다. 주진단은 ADHD가 가장 많았고. 불안장애, 정신지체, 우울증, 반항장애, 발달성언어장애 등의 순이었다. 두 가지 이상의 진단이 내려진 경우가 48.9% 이었고, 부진단으로는 유뇨증, 품행장애, 발달성언어장애 등이 있었다. 주진단을 ADHD로 받은 환아들에서 공존질병이 있는 경우가 55.3%에 달했고. 공존질병은 특수발달장애, 품행장애, 반항장애, 불안장애 등의 순이었다. 순수 ADHD군에 비해서 공존질병 수반군에서는 동작성 지능검사와 연속과제수행검사에서 저조한 수행을 보였다. 외향적장애군은 ADHD군에 비해 지능검사의 상식 소검사 및 같은 그림찾기 검사와 연속과제수행검사에서 수행의 저하를 보였다. 내향적장애군은 ADHD군에 비해 교사 평정척도 점수가 낮았고, 아동행동조사표의 소통불능요인이 높았으며 연속과제수행검사에서는 카드 오류수가 낮았다. 본 연구의 결과를 볼 때 주의산만이나 과잉운동이 주증상으로 나타날 경우 각종 평가 및 신경심리검사도구를 광범위하게 사용하여 ADHD 외에도 외향적 및 내향적장애군 등 다양한 질환을 감별하여야 하며, ADHD 진단을 내리는 경우에도 수반된 공존질병의 유무를 확인하여 이를 치료계획에 반영하여야 할 것으로 생각된다.

• 대한물리치료과학회지
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• 제10권2호
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• pp.62-72
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• 2003

The purpose of this study is to prove the efficacy of the Kaltenborn-Evjenth Orthopedic Manipulative Therapy and the Conventional Physical Therapy that influence changes in pain and range of motion when those therapies are applied to patients with temporomandibular pint movement restriction. The subjects of the study were 30 randomly selected patients who had been diagnosed with temporomandibular pint movement restriction and had endured pain for more than two weeks. These patients had visited one of three hospitals in Pyongchon 00 Hospital. The subjects were divided into two groups with 15 patients each. The Kaltenborn-Evjenth Orthopedic Manipulative Therapy was applied to one group and the Conventional Physical Therapy was applied to the other group, once a day for three days. Then, the pain perception degree was measured by using the Visual Analogue Scale(VAS) and a digital device(Absolute Digimatic) measured the range of motion for each group. The average and standard errors were calculated for each measured items and a paired t-test was used for identifying significance in the differences in the pain perception degree and the range of motion between the two groups according to therapy. The significant level was set as ${\alpha}=0.05$. The changes in the pain perception degree were statistically significant in both groups; however, the group that received Kaltenborn-Evjenth Orthopedic Manipulative Therapy(KE-Group) showed more significant changes in a decrease in the pain perception degree than did the group that received the Conventional Physical Therapy(CPT-Group). Both groups showed significant results regarding changes in the range of motion: however, the KE-Group showed more of a significant difference in the average of the range of motion than did the CPT-Group(p<0.001). Comparing the changes in the range of motion between the two groups, the KE-Group showed a significant result which means that the KE-Group had a higher therapy effect than did the CPT-Group(p<0.05). Based on the results of this study, we found that the Kaltenborn-Evjenth Orthopedic Manipulative Therapy decreased pain and increased the range of motion. With such findings, we expect that the Kartenborn-Evjenth Orthopedic Manipulative Therapy can be used as an effective treatment method for patients with tempomrnandibular pint movement restriction and that the treatment period can be reduced with this therapy as well.

• The Korean Journal of Physiology and Pharmacology
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• 제8권2호
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• pp.95-100
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• 2004

Ischemic preconditioning (IPC) has been accepted as a heart protection phenomenon against ischemia and reperfusion (I/R) injury. The activation of ATP-sensitive potassium $(K_{ATP})$ channels and the release of myocardial nitric oxide (NO) induced by IPC were demonstrated as the triggers or mediators of IPC. A common action mechanism of NO is a direct or indirect increase in tissue cGMP content. Furthermore, cGMP has also been shown to contribute cardiac protective effect to reduce heart I/R-induced infarction. The present investigation tested the hypothesis that $K_{ATP}$ channels attenuate DNA strand breaks and oxidative damage in an in vitro model of I/R utilizing rat ventricular myocytes. We estimated DNA strand breaks and oxidative damage by mean of single cell gel electrophoresis with endonuclease III cutting sites (comet assay). In the I/R model, the level of DNA damage increased massively. Preconditioning with a single 5-min anoxia, diazoxide $(100\;{\mu}M)$, SNAP $(300\;{\mu}M)$ and 8-(4-Chlorophenylthio)-guanosine-3',5'-cyclic monophosphate (8-pCPT-cGMP) $(100\;{\mu}M)$ followed by 15 min reoxygenation reduced DNA damage level against subsequent 30 min anoxia and 60 min reoxygenation. These protective effects were blocked by the concomitant presence of glibenclamide $(50\;{\mu}M)$, 5-hydroxydecanoate (5-HD) $(100\;{\mu}M)$ and 8-(4-Chlorophenylthio)-guanosine-3',5'-cyclic monophosphate, Rp-isomer (Rp-8-pCPT-cGMP) $(100\;{\mu}M)$. These results suggest that NO-cGMP-protein kinase G (PKG) pathway contributes to cardioprotective effect of $K_{ATP}$ channels in rat ventricular myocytes.

• Smart Structures and Systems
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• 제26권3호
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• pp.331-343
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• 2020

In this paper, based on the CPT, motion equations for a sandwich plate containing a core and two integrated face-sheets have derived. The structure rests on the Visco-Pasternak foundation, which includes normal and shear modules. The piezo-magnetic core is made of CoFe₂O₄ and also is subjected to 3D magnetic potential. Two face sheets at top and bottom of the core are under electrical fields. Also, in order to obtain more accuracy, the effect of flexoelectricity has took into account at face sheets' relations in this work. Flexoelectricity is a property of all insulators whereby they polarize when subject to an inhomogeneous deformation. This property plays a crucial role in small-scale rather than macro scale. Employing CPT, Hamilton's principle, flexoelectricity considerations, the governing equations are derived and then solved analytically. By present work a detailed numerical study is obtained based on Piezoelectricity, Flexoelectricity and modified couple stress theories to indicate the significant effect of length scale parameter, shear correction factor, aspect and thickness ratios and boundary conditions on natural frequency of sandwich plates. Also, the figures show that there is an excellent agreement between present study and previous researches. These finding can be used for automotive industries, aircrafts, marine vessels and building industries.

• 생약학회지
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• 제27권1호
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• pp.42-46
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• 1996

The medicinal formula, Kilkyungtang (KKT) and two modified Kilkyungtangs (KKT-1 and KKT-2), which were supplemented by the additional crude drug, Houttuyniae herba (KKT-1), and Oldenlandiae diffusae herba (KKT-2) to KKT, had been applied widely as decoctions for the treatment of malignant tumors. Cytotoxic activities against two tumor cell lines, A549 and $B16-F_0$ were investigated. However, none of them were found to exhibit significant cytotoxicity upon tested tumor cells below the concentration of $1000{\mu}g/ml$. However, cytotoxic activities of three reputed antitumor agents, i.e., mitomycin C (MMC), cisplatin (CPT) and 5-fluorouracil (5-FU) was significantly potentiated by the combined treatment of them with KKT, KKT-1 and KKT-2 respectively, especially against A549 (human non small cell lung adenocarcinoma), in vitro.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 춘계학술대회
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• pp.31-35
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• 1998

A series of 2-oxiranecarboxylate derivatives was prepared as carnitine palmitoyl transferase I (CPT- -I) inhibitors for the development of new antidiabetic agents. The syntheses and biological activities were reported. The most promising derivative, 15b showed 2.5 times more hypoglycemic activity and 2 times lower acute toxicity compared to Etomoxir.

• Elastomers and Composites
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• 제13권3호
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• pp.197-206
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• 1978

반응속도면(反應速度面)에서의 동력학적(動力學的) 연구결과(硏究結果)에 의(依)하면 2-메르캅토벤쪼티아졸(MBT)은 N-t-부틸-벤쪼티아졸썰펜아미드(BBTS) 또는 2-(4-몰폴리노티오) 벤쪼티아졸(OBTS)과 같은 가황촉진제(加黃促進劑)들 보다는 N-(싸이클로헥실티오)프탈이미드(CPT)가 훨신 더 빨리 반응(反應)한다는 사실(事實)을 탐지(探知)하였다. 또한 우수(優秀)한 가황지연제(加黃遲延劑)는 MBT와 급속(急速)히 반응(反應)하여야 하지마는 실소(室素)와 황(黃)과의 화학결합(化學結合)이 너무 약(弱)하여 내열안정성(耐熱安定性)이 없음으로 아무리 MBT와 빠른 속도(速度)로 반응(反應)하여도 좋은 가황(加黃)지연제가 될 수는 없다. 예를들면 N-(싸이클로헥실티오-O-벤쪼의 썰펜이미드(CTBS))는 CTP보다 더 빨리 MBT와 반응(反應)하지만 열안정성(熱安定性)이 부족(不足)하여 가황(加黃)지연제로서는 CTB 보다 좋지 못하다.