• KSBB Journal
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• 제16권1호
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• pp.66-70
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• 2001

순수한 초임계 이산화탄소를 이용하여 camptothecin(CPT)와 10-hydroxy camptothecin(HCPT) 추출 시험을 결과, 이들은 각각 유기용매 메탄올 추출에 비교하여 3%와 2% 밖에 추출되지 않았다. 보조용매로 메탄올과 에탄올을 이용하여 CPT와 HCPT를 추출하면 추출 효율이 상승됨을 확인할 수 있었다. 특히 에탄올 보다 메탄올이 효율이 좋았다. 따라서 보조용매 적용에 관련된 여러 조건들을 최적화 하였다. 메탄올을 보조용매로 사용하는 경우에 보조용매의 농도가 증가할수록 추출 효율도 증가하였고 16% 이상에서는 추출 효율이 완만하게 증가하였다. 또한 추출은 온도에 따라 크게 영향을 받지 않아으나 온도에 따라 약간 증가하는 경향을 보였다. 초임계 추출에서 압력의 영향을 살펴본 결과 250 bar까지 압력이 증가할수록 추출 효율은 증가하였다. Acetate ion을 공급하여 추출 실험을 수행하였으나 CPT나 HCPT 모두 ion pairing agent의 영향은 없었다. 본 시스템과 같은 solid/fluid 추출 시스템에서 추출 효율에 영향을 크게 미치는 3가지 요소 (용해도, 확산, 및 matrix)중 확산의 영향을 살펴보았다. 여러 기하학적 모델 중 matrix가 구형인 hot-ball model로 확산게수를 계산하였다. 본 시스템에서 보조용매를 포함한 초임계 유체의 $D/r^2$ 값은 0.0072 $min^{-1}$로서 순수한 메탄올 유기용매 추출보다 큰 값을 보였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제36권3호
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• pp.177-185
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• 2010

Introduction: Epidermal growth factor receptor (EGFR) is expressed in human epithelial tumors including salivary cancers, and known to be correlated with tumor progression and poor clinical courses in some epithelial tumors. In this study, we determined the therapeutic effect of the anti-EGFR monoclonal antibody Erbitux (C225, cetuximab) in combination with the DNA topoisomerase I inhibitor irinotecan (CPT-11) on human salivary adenoid cystic carcinoma (SACC) cells growing in nude mice. Materials and Methods: At first, immunocytochemical analysis for the expression of EGFR and phosphorylated EGFR (pEGFR) on a human salivary ACC cell line (ACC3). To determine the in vivo effects of Erbitux and CPT-11, nude mice with orthotopic parotid tumors were randomized to receive intraperitoneal Erbitux (1 mg) two times per week, intraperitoneal Irinotecan (50 mg/kg) once per week, Erbitux plus CPT-11, or placebo. (control) Tumor volume and weight were measured. And mechanisms of in vivo activity of Erbitux and/or CPT-11 were determined by immunohistochemical/ immunofluorescent analyses. Results: Immunocytochemical staining of ACC3 demonstrated that EGFR was expressed and phosphorylated. CPT-11 inhibited ACC tumor growth in nude mice. Tumors of mice treated with CPT-11 and CPT-11 plus Erbitux exhibited increased tumor cell apoptosis and decreased microvessel density, which correlated with a decrease in the tumor volume in nude mice. But, CPT-11 seems not to be synergistic with Erbitux in our ACC3 model system. Conclusion: These results suggest that anti-EGFR monoclonal antibody and the DNA topoisomerase I inhibitor will be effective in the treatment of recurred or metastatic lesions of salivary ACC.

• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2439-2445
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• 2014

Cryptotanshinone (CPT), is a quinoid diterpene isolated from the root of the Asian medicinal plant, Salvia miotiorrhiza bunge. Numerous researchers have found that it could work as a potent antitumor agent to inhibit tumor growth in vitro, buith there has been much less emphasis on its in vivo role against breast tumors. Using a mouse tumor model of MCF7 cells, we showed that CPT strongly inhibited MCF7 cell growth in vivo with polarization of immune reactions toward Th1-type responses, stimulation of naive CD4+ T cell proliferation, and also increased IFN-${\gamma}$ and perforin production of CD4+ T cells in response to tumor-activated splenocytes. Furthermore, data revealed that the cytotoxic activity of CD4+ T cells induced by CPT was markedly abrogated by concanamycin A(CMA), a perforin inhibitor, but not IFN-${\gamma}$ Ab. On the other hand, after depletion of CD4+ T cells or blocked perforin with CMA in a tumor-bearing model, CPT could not effectively suppress tumor growth, but this phenomenon could be reversed by injecting naive CD4+ T cells. Thus, our results suggested that CPT mainly inhibited breast tumor growth through inducing cytotoxic CD4+ T cells to secrete perforin. We further found that CPT enhanced perforin production of CD4+ T cells by up-regulating JAK2 and STAT4 phosphorylation. These findings suggest a novel potential therapeutic role for CPT in tumor therapy, and demonstrate that CPT performs its antitumor functions through cytotoxic CD4+ T cells.

• Journal of Oral Medicine and Pain
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• 제33권1호
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• pp.97-103
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• 2008

본 연구는 부산대학병원 구강내과로 내원하여 삼차신경통으로 진단되어 사전에 실험에 대한 동의를 한 환자 20명(여자 13명, 남자 7명, $42{\sim}77$세, 평균$58.20{\pm}16.99$세)을 연구대상으로 저출력레이저 조사전,후 전류인지역치검사를 하여 다음과 같은 결과를 얻었다. 1. 레이저 조사전,후 이환측과 비이환측의 CPT값의 차이는 없었다. 2. 레이저 조사후 이환측과 비이환측의 CPT값 변화에 유의성은 없었다. 3. 하지만 레이저 조사후 이환측과 비이환측의 $A{\beta}$섬유의 경우 CPT값이 증가하는 경향을 보였다.

• 한국식품과학회지
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• 제48권5호
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• pp.486-491
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• 2016

DBD 방식의 콜드 플라즈마 처리는 양파 분말에 접종된 E. coli O157:H7, S. Enteritidis, L. monocytogenes를 효과적으로 저해시켰다. 콜드 플라즈마 처리에 의한 식중독균 억제 효과는 콜드 플라즈마 전압 증가에 따른 유의적인 차이를 보이지 않았지만, 콜드플라즈마 처리 시간에 따라 억제 효과가 유의적으로 증가함을 알 수 있었다. 또한, 양파 분말의 수분활성도가 높은 것과 입자 크기가 큰 것이 콜드 플라즈마를 이용하여 미생물을 억제 시키는데 유리하다는 것을 규명하였다. 본 연구에서 사용된 처리 조건에서의 DBD-CPT는 처리 시간과 관계없이 양파 분말의 비타민C 함량, 쿼세틴 함량, 그리고 색도에 영향을 주지 않았다. 따라서 DBD-CPT는 양파 분말의 이화학적 특성에 영향을 미치지 않으면서 CP 처리 시간, 양파 분말의 수분활성도와 크기 조절을 통해 식중독균을 효과적으로 억제 시킬 수 있음을 알 수 있었고 이로써 DBD-CPT가 양파 분말 식중독균 저감화에 사용 될 수 있는 비가열 살균 기술임을 확인 할 수 있었다.

• 한국초전도ㆍ저온공학회논문지
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• 제15권1호
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• pp.40-44
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• 2013

The contactless power transfer (CPT) systems have been recently gaining popularity widely since it is an available option to realize the power delivery and storage with connector-free devices across a large air gap. Especially, the CPT with electromagnetic resonance coupling method is possible to exchange energy within 2 m efficiently. However, the power transfer efficiency of CPT in commercialized products has been limited because the impedance matching of coupled coils is sensitive. As a reasonable approach, we combined the CPT system with HTS wire technology and called as, superconducting contactless power transfer (SUCPT) system. Since the superconducting coils have an enough current density, the superconducting antenna and receiver coils at CPT system have a merit to deliver and receive a mass amount of electric energy. In this paper, we present the feasibility of the SUCPT system and examine the transmission properties of SUCPT phenomenon between room temperature and very low temperature at 77 K as long as the receiver is within 1.0 m distance.

• 한국산학기술학회논문지
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• 제16권1호
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• pp.445-452
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• 2015

경두개직류전류자극(tDCS)은 낮은 직류 전류 강도를 사용하여 대뇌피질의 자발적인 신경학적 활동의 흥분성을 증가 또는 감소시키는 신경조절 기법이다. 본 연구의 목적은 tDCS를 적용한 후 다양한 감각 기능의 변화를 측정하는데 있다. tDCS의 효과를 측정하기 위해 CPT 검사를 50명의 건강한 대상자에게 단일 기간(8월4일에서 8월29일), 단일 공간, 단일 맹검법으로 무작위 배정하였다. 신경전도검사는 우세 손의 말초신경 병변을 구별하기 위해 정중 감각과 운동신경을 측정하였다. 대상자들은 각 25명 씩 대뇌피질의 DLPFC의 tDCS 자극군과 대뇌피질의 DLPFC의 tDCS 위자극군으로 2개의 다른 조건 아래서 1 mA의 전류강도로 15분씩 양극 tDCS로 적용하였다. 촉각, 빠른 통증과 느린 통증을 각각 평가하기 위해 우세한 제 2수지에 2000, 250, 그리고 5 Hz의 주파수로 CPT 검사인 Neurometer$^{(R)}$를 이용하여 수치들을 기록하였다. DLPFC의 양극 tDCS 자극군의 CPT 수치들에서는 250과 5 Hz에서 통계적으로 유의한 증가를 보였다. 양극 tDCS 위자극군의 모든 CPT 수치들은 감소하였다. 이러한 결과는 DLPFC의 양극 tDCS가 건강한 대상자들의 감각 지각과 통증 역치들을 조절할 수 있다는 것을 보여준다. 따라서 본 연구는 재활과 통증 치료 분야에서 유용한 치료 방법 중 하나로 제시할 수 있을 것으로 생각한다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제7권2호
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• pp.405-424
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• 2013

In publishing applications, it is advantageous to embed data in halftone images. The CPT scheme (Chen-Pan-Tseng, 2000) is a steganographic data hiding scheme that was proposed for binary images, e.g., facsimiles. The CPT scheme uses a secret key and weight matrix to protect the hidden data and can hide as many as $r={\lfloor}{\log}_2(m{\times}n+1){\rfloor}$ bits of data in the image by changing at most 2 bits in the image. Our proposed scheme also uses a secret key to protect it from being compromised and a weight matrix to increase the data hiding rate. Our scheme improves the performance of the CPT scheme by using the simple principle of splitting each block into two parts. Our proposed scheme is called improved CPT (ICPT) and has a very high embedding capacity compared to previous schemes. Experimental results demonstrate that our proposed scheme generally has higher performance than previous schemes.

• Geomechanics and Engineering
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• 제31권1호
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• pp.113-127
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• 2022

The undrained shear strength of soil is considered one of the engineering parameters of utmost significance in geotechnical design methods. In-situ experiments like cone penetration tests (CPT) have been used in the last several years to estimate the undrained shear strength depending on the characteristics of the soil. Nevertheless, the majority of these techniques rely on correlation presumptions, which may lead to uneven accuracy. This research's general aim is to extend a new united soft computing model, which is a combination of random forest (RF) with grasshopper optimization algorithm (GOA) to the pile set-up parameters' better approximation from CPT, based on two different types of data as inputs. Data type 1 contains pile parameters, and data type 2 consists of soil properties. The contribution of this article is that hybrid GOA - RF for the first time, was suggested to forecast the pile set-up parameter from CPT. In order to do this, CPT data and related bore log data were gathered from 70 various locations across Louisiana. With an R² greater than 0.9098, which denotes the permissible relationship between measured and anticipated values, the results demonstrated that both models perform well in forecasting the set-up parameter. It is comprehensible that, in the training and testing step, the model with data type 2 has finer capability than the model using data type 1, with R² and RMSE are 0.9272 and 0.0305 for the training step and 0.9182 and 0.0415 for the testing step. All in all, the models' results depict that the A parameter could be forecasted with adequate precision from the CPT data with the usage of hybrid GOA - RF models. However, the RF model with soil features as input parameters results in a finer commentary of pile set-up parameters.

• 대한구순구개열학회지
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• 제4권1호
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• pp.39-43
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• 2001

The design of osteotomy plane in orthognathic surgery has been developed to diminish the nerve injury. Intraoral Vertico-Sagittal Ramus Osteotomy (IVSRO) is the one of the best way to minimize untoward results, which is designed not to expose the lingula. We evaluated the nerve damage before and after with current perception threshold (CPT) test which is modem and numerically expressible way of nerve damages. Sixty patients underwent IVSRO since 1998 were evaluated. They were divided into 2 groups; one group underwent IVSRO only, and the other underwent IVSRO plus genioplasty. The both groups were evaluated with CPT test 1 week before surgery, and 1, 3 and 6 months after surgery. The CPT test was performed on A-beta, A-delta and C fiber respectively. 111e result showed that the recovery of sensory function of damaged nerve fibers was observed at the period of three to six months after surgery. There was no impairment of nerve function after only the IVSRO . But there were sensory disturbances in cases of additional genioplasty group. We thought that one of major factors on nerve damages were exposure of nerve and traction injury during genioplasty.