• Title/Summary/Keyword: CPF complex

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Spectrophotometric Investigation of Germanium Complex Solution with o-Chlorophenylfluorone and Determination of Trace Amounts of Germanium

  • Hong-Wen Gao;Wei-Guo Liu
    • Bulletin of the Korean Chemical Society
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    • v.21 no.11
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    • pp.1090-1094
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    • 2000
  • A reaction between germanium (Ge) and the ligand, o-chlorophenylfluorone (o-CPF) has been carried out. The reaction sensitive at pH 4.5 in the presence of triton x-100 was selective in the presence of EDTA. The spectral correction technique was ap plied to the analysis of the reaction instead of single wavelength spectrophotometry because the absorption of excess of o-CPF was not negligible. An updated determination of the properties of the Ge(IV)-o-CPF complex is given, which involved the complex ratio, stepwise absorptivity and stability constant of the complex. In present work, the results show that the complex $Ge(o-CPF)_3was$ formed and its cumulative stability constant was 1.09 ${\times}$1016 . For sample analysis, the detection limit of germanium was 0.01 mg/L, and the recoveries were between 96.4% and 102%, with relative standard deviations of less than 6.5%.

Spectrophotometric Investigation of Cu and Fe Complexes with o-Chlorophenylfluorone and Selective Competition Coordination Determination of Iron Traces

  • Gao, Hong-Wen
    • Bulletin of the Korean Chemical Society
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    • v.23 no.7
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    • pp.943-947
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    • 2002
  • The two reactions between iron ion (Fe3+ ) and o-chlorophenylfluorone (o-CPF) and copper ion (Cu2+ ) and o-CPF are sensitive at pH 6 in the presence of Triton X-100. We have determined the formation constants of the complexes by the spectral correction technique. Because of the poor selectivity of o-CPF to metals, the competition coordination of only the iron ion from the Cu-o-CPF complex was found and applied to the selective detection of iron traces by the Selective Competition Coordination Determination (SCCD) approach.The analysis of several samples shows that the relative standard deviations are less than 5.0% and the recovery of iron ions between 94.5% and 106%.

The effect of Swd2's binding to Set1 on the dual functions of Swd2 in Saccharomyces cerevisiae (Saccharomyces cerevisiae의 Swd2와 Set1의 결합이 Swd2의 이중적인 기능에 미치는 영향)

  • Park, Shinae;Lee, Jung-Shin
    • Korean Journal of Microbiology
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    • v.53 no.4
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    • pp.286-291
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    • 2017
  • In eukaryotic cells, histone modification is an important mechanism to regulate the chromatin structure. The methylation of the fourth lysine on histone H3 (H3K4) by Set1 complex is one of the various well-known histone modifications. Set1 complex has seven subunits including Swd2, which is known to be important for H2B ubiquitination dependent on H3K4 methylation. Swd2 was reported to regulate Set1's methyltransferase activity by binding to near RNA recognition motif (RRM) domain of Set1 and to act as a component of CPF (Cleavage and Polyadenylation Factors) complex involved in RNA 3' end processing. According to the recent reports, two functions of Swd2 work independently of each other and the lethality of Swd2 knockout strain was known to be caused by its function as a component of CPF complex. In this study, we found that Swd2 could influence the Set1's stability as well as histone methyltransferase activity through the association with RRM domain of Set1. Also, we found that ${\Delta}swd2$ mutant bearing truncated-Set1, which cannot interact with Swd2, lost its lethality and grew normally. These results suggest that the dual functions of Swd2 in H3K4 methylation and RNA 3' end processing are not independent in Saccharomyces cerevisiae.

A design of Adaptive Decision-feedback Equalizer Module using Redundant Binary Complex Filter (Redundant Binary 복소수 필터를 이용한 적응 결정귀환 등화기 모듈 설계)

  • 김호하;안병규신경욱
    • Proceedings of the IEEK Conference
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    • 1998.10a
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    • pp.1125-1128
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    • 1998
  • A new architecture for high-speed implementation of adaptive decision-feedback equalizer (ADFE) applicable to wide-band digital wireless modems is described. Rather than using conventional two's complement arithmetic, a novel complex-valued filter structure is devised, which is based on redundant binary (RB) arithmetic. The proposed RB complex-valued filter reduces the critical path delay of ADFE, as well as leads to a more compact implementation than conventional methods. Also, the carry-propagation free (CPF) operation of the RB arithmetic enhances its speed. To demonstrate the proposed method, a prototype chip set is designed. They are designed to contain two complexvalued filter taps along with their coefficient updating circuits, and can be cascaded to implement loger filter taps for high bit-rate applications.

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Calculate Transmission Reliability Margin using Probabilistic Load Flow (확률론적 조류계산을 이용한 송전 신뢰도 여유 계산)

  • Shin, Dong-Joon;Kim, Jin-O;Kim, Kyu-Ho
    • Proceedings of the KIEE Conference
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    • 2003.07a
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    • pp.569-571
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    • 2003
  • As a definition of NERC, Available Transfer Capability(ATC) is a measure of the transfer capability remaining in the physical transmission network for the future commercial activity. To calculate ATC, accurate and defensible TTC, CBM and TRM should be calculated in advance. In this paper, we propose a method to quantify TRM using probabilistic load flow based on the method of moment. Generation output, bus voltages, loads, and line outages are considered as complex random variables (CRV) to take into account for uncertainties related to the transmission network conditions. Probability Density Function (PDF) of line flow at the most limiting line is used to quantify TRM with the desired probabilistic margin. Suggested method is compared with the results from conventional CPF method and verified using 24 bus MRTS, and the suggested method based on PLF shows efficiency and flexibility for the quantification of TRM compared with the conventional method.

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Isolation, Purification and Characterization of Polysaccharides that induce in vitro Immuno-Stimulation of Macrophases derived from Liquid Culture of Cordyceps militaris

  • Kwon, Jeong-Seok;An, Hyo-Sil;Hong, Eock-Kee
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.287-292
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    • 2005
  • The crude polysaccharides(C-CPF, C-CPM, C-CPB) derived from fruiting body, mycelia and mycelia free broth of cordyceps militaris were obtained by ethanol precipitation of hot water extracts. After a batch fermentation of C. militaris was carried out in a 5 L jar vessel, endo-polysaccharide and exo-polysaccharide were obtained. They were demonstrated as the hetero polysaccharides which were composed of glucsose, galactose and mannose by performed with HPAEC(high pH anion exchange chromatography) and conformation of random coil by its complex forming ability with congo red reagent. They were purified by ion exchange (DEAE-cellulose) and gel filtration chromatography. They were monitered by phenol-sulfuric acid method and Bradford method. The NO induction activities of crude polysaccharides and purified polysaccharides derived from mycelia free broth were enhanced rather than LPS(lipo polysaccharide) which was used as a general NO inducer. These effects presumably contibute to the antitumor activities. The homogenieties and molecular weights of polysaccharides were determined by using Sepharose CL-6B. The yield, molecular weights and NO induction activities of C-CPFN Fr.III, C-CPMN Fr.III, C-CPBN Fr.II were 0.387, 0.408 and 0.153, 127 K 210 K and 36 K, 40.79%, 88.72%, and 104.17%, respectively.

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Estimation of Contribution by Pollutant Source of VOCs in Industrial Complexes of Gwangju Using Receptor Model (PMF) (수용모델(PMF)을 이용한 광주산업단지 VOCs의 오염원별 기여도 추정)

  • Park, Jin-Hwan;Park, Byoung-Hoon;Kim, Seung-Ho;Yang, Yoon-Cheol;Lee, Ki-Won;Bae, Seok-Jin;Song, Hyeong-Myeong
    • Journal of Environmental Science International
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    • v.30 no.3
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    • pp.219-234
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    • 2021
  • Industrial emissions, mainly from industrial complexes, are important sources of ambient Volatile Organic Compounds (VOCs). Identification of the significant VOC sources from industrial complexes has practical significance for emission reduction. VOC samples were collected from July 2019 to June 2020. A Positive Matrix Factorization (PMF) receptor model was used to evaluate the VOC sources in the area. Four sources were identified by PMF analysis, including coating-1, coating-2, printing, and vehicle exhaust. The coating-1 source was revealed to have the highest contribution (41.5%), followed by coating-2 (23.9%), printing (23.1%), and vehicle exhaust (11.6%). The source showing the highest contribution was coating emissions, originating from the northwest to southwest of the sample site. It also relates to facilities that produce auto parts. The major components of VOC emissions from the coating facilities were toluene, m,p-xylene, ethylbenzene, o-xylene, and butyl acetate. Industrial emissions should be the top priority to meet the relevant control criteria, followed by vehicular emissions. This study provides a strategy for VOC source apportionment from an industrial complex, which is helpful in the development of targeted control strategies.