• Asian-Australasian Journal of Animal Sciences
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• v.6 no.2
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• pp.253-258
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• 1993

The study on the nutritive value and the potential use of two kinds (local vs. import) of sesame meal (SSM) in layer diets as substitute for soybean meal (SBM) at 25, 50, and 75% was carried out. Three hundred and thirty six layers were kept individually on a battery cage and fed isonitrogenous diets, 16% crude protein (CP), for 252 days. The local and the imported SSM contained on air dry basis 35.7 and 36.3% CP, 24.7 and 9.1% ether extract (EE), respectively. Their amino acid contents are all lower than those reported by NRC (1984). The local material contained remarkably lower lysine (0.44 vs. 0.94), methionine + cystine (1.06 vs. 1.75) and threonine (0.52 vs. 1.22) than the imported meal. Imported SSM could be substituted for SBM as high as 50% in layer ration without statistically adverse effect on egg production, feed intake, body weight gain, average egg weight and egg size, while local SSM was inferior to the control in all aspects. However, when production performance of the groups fed either kind of SSM was compared, no significant difference was found at the two lower substitution levels.

• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.4
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• pp.1-8
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• 2000

This study is conducted to evaluate the effects of root restriction on growth of maple(Acer palmatum Thumb.). Tow types of container such as clay pot(CP), root control bag(RCB) were used to restrict maple's root and each type of container was divided into 5 sizes. The containers with plants were buried just below the soil level and maples planted directly in the soil (nonrestricted root treatment) were included as comparison. Data were collected on dry weight of leaf, trunk, thick root, rootlet and soil water potential. We have analyzed, simple linear regression, Pearson's Correlation analysis, Duncan's multiple rang test, and Covariance Analysis using SAS statistical software. The results of analysis based on these data are as follows; 1. Total dry weight of maple in CP was significantly larger than in RCB. 2. Difference in growth reduction by the kinds of containers was induced by different hydraulic diffusion ratios between container and soil. Difference in growth reduction by the size of container was induced by the difference density of rootlet and soil moisture contents in the container. 4. Commercial products of root control of root control bag appeared not proper for countries in which fluctuation of rainfall is severe. Because maples in RCB were restricted by excess soil water in the rainy season, or by lack of soil water in the dray season.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.379-385
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• 2014

The purpose of this study was to compare the ability of an engineered Escherichia coli to degrade chlorpyrifos (Cp) using an organophosphorus hydrolase enzyme, encoded in both Flavobacterium sp. ATCC 27551 or Pseudomonas diminuta, by employing the Lpp-OmpA chimera and the N-terminal domain of the ice nucleation protein as anchoring motifs. Tracing of the expression location of the recombinant protein using SDS-PAGE showed the presentation of OPH by both anchors on the outer membrane. This is the first report on the presentation of OPH on the cell surface by Lpp-OmpA under the control of the T7 promoter. The results showed cell growth in the presence of Cp as the sole source of energy, without growth inhibition, and with higher whole-cell activity for both cells harboring plasmids pENVO and pELMO, at approximately 10,342.85 and 10,857.14 U/mg, respectively. Noticeably, the protein displayed by pELMO was lower than the protein displayed by pENVO. It can be concluded that Lpp-OmpA can display less protein, but more functional OPH protein. These results highlight the high potential, of both engineered bacteria, for use in the bioremediation of pesticide-contaminated sources in the environment.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.8
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• pp.1143-1150
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• 2003

This study was conducted to investigate in vitro methane production of feed ingredients and relationship between the content of crude nutrients and methane production. Feed ingredients (total 26) were grouped as grains (5 ingredients), brans and hulls (8), oil seed meals (9) roughages (3), and animal by-product (1) from their nutrient composition and their methane production protential were measured by in vitro gas test. Among the groups, the in vitro methane productions for both 6 and 24 h incubation were highest in grains, followed by brans and hulls, oil meals and roughages, animal byproducts. Within the group of grains, methane production from wheat flour was the highest, followed by wheat, corn, tapioca, and then oat. Within the brans and hulls, soybean hull showed the highest methane production and cotton seed hull, the lowest. Methane production from oil meals was lower compared with grains and brans and hulls, and in decreasing order production from canola meal was followed by soybean meal, coconut meal, and corn germ meal (p<0.01). Three ingredients were selected and the interactions among feed ingredients were evaluated for methane production. Correlation coefficient between measured and estimated values of the combinations were 0.91. Methane production from each feed ingredient was decreased with increasing amount of crude fiber (CF), protein (CP) and ether extract (EE), whereas positive relationship was noted with the concentrations of N-free extract (NFE). The multiple regression equation (n=134) for methane production and nutrient concentrations was as follows. Methane production (ml/0.2 g DM)=(0.032${\times}$CP)-(0.057${\times}$EE)-(0.012${\times}$CF)+(0.124${\times}$NFE) (p<0.01; $R^2$=0.929). Positive relationship was noted for CP and NFE and negative relationship for CF and EE. It seems possible to predict methane production potential from nutritional composition of the ingredients for their effective application on formulating less methane emitting rations.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.6
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• pp.844-850
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• 2002

The potential nutritive value was studied on leaves of seven fodder trees in Central Sudan during dry season at two distinct periods, the early dry and the late. The chemical composition, mineral concentration, in vitro organic matter digestibility (IVOMD), in situ OM or nitrogen degradability and estimated metabolizable energy showed a wide variation among fodder tree species and between different periods of the dry season. Crude protein (CP) ranged from 285 to 197 g/kg DM at early dry season, with a significant reduction in late dry season. Ziziphus spina-christi and Balanites aegyptiaca showed the least reduction in CP content. The NDF, ADF and lignin were about 200, 160 and 19 g/kg DM, respectively at the early period, and significantly increased at the late period of the dry season, except for lignin of Z. spina-christi. For mineral concentration, all fodder tree leaves were rich in calcium but poor in phosphorus. In situ OM degradability significantly decreased at the late period of dry season, but values remained as high as over 600 g/kg OM. At both periods, Z. spina-christi showed the highest value, while the lowest was recorded in Acacia seyal. The IVOMD showed a similar trend to those of in situ OM degradability, except for A. seyal. The nitrogen degradability was highest in B. aegyptiaca and lowest in Z. spina-christi at both periods. A significant and positive correlation had existed between CP and IVOMD or in situ OM degradability (r=0.68, p<0.05; r=0.77, p<0.05, respectively). Also, a significant but negative correlation was found between condensed tannins and nitrogen degradability (r=-0.87, p<0.01). Results demonstrated that Z. spina-christi potentially has a good nutritive value as dry season feed or supplement, while A. seyal tends to be less promising. A. nubica and B. aegyptiaca may be a useful source for degradable protein, even though it may have a limited supply of energy to animals. A. tortilis, A. mellifera and A. ehrenbergiana may have potential value for a supplementation of energy or protein, if they were harvested in the early dry season or in wet season as preserved feed. It is highly recommended to supplement with an appropriate amount of phosphorus when these fodder trees were used.

• Korean Journal of Environmental Agriculture
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• v.36 no.1
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• pp.29-35
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• 2017

BACKGROUND: This study was carried out to assess a biochemical methane potential of giant miscanthus (Miscanthus sacchariflorus) which was a promising candidate energy crop due to a high biomass productivity, in order to utilize as a feedstock for the biogas production. METHODSANDRESULTS: Giant miscanthus was sampled the elapsing drying time of 6 months after harvesting. TS (Total Solid) and VS (Volatile Solid) contents were 94.7 and 90.8%. And CP (Crude Protein), EE (Ether Extracts), and CF (Crude Fiber) contents of giant miscanthus were 1.4, 0.46, and 46.12%, respectively. In the organic composition of giant miscanthus, the NDF (Neutral Detergent Fiber) representing cellulose, lignin, and hemicellulose contents showed 86.88%, and the ADF (Acid Detergent Fiber) representing cellulose and lignin contents was 62.91%. Elemental composition of giant miscanthus showed 47.75%, 6.44%, 41.00%, and 0.28% for C, H, O, and N, respectively, and then, theoretical methane potential was obtained to $0.502Nm^3kg^{-1}-VS_{added}$. Biochemical methane potential was assessed as the range of $0.154{\sim}0.241Nm^3kg^{-1}-VS_{added}$ resulting the lower organic biodegradability of 30.7~48.0%. CONCLUSION: Therefore the development of pretreatment technology of the giant miscanthus was needed for the improvement of anaerobic digestability.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.11
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• pp.1721-1728
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• 2007

An in vitro and a feeding trial were conducted to investigate the effect of xylanase supplementation on the feeding value of growing pig diets containing high proportions of Chinese double-low rapeseed meals (DLRM). Seven diets were formulated to meet NRC (1998) nutrient requirements. Diet 1 based on corn-soybean meal was used as positive control 1, and diet 2, a practical diet which incorporated a conventional level of Chinese DLRM (60 g/kg diet), as positive control 2. Diet 3 contained a higher level of DLRM (100 g/kg diet) as the negative control. Diet 3 plus xylanase at 0.10, 0.25, 0.50 and 0.70 g/kg diet created diets 4, 5, 6 and 7, respectively. The seven diets were incubated in triplicate with the in vitro two-stage enzyme incubation method to predict responses of diets to xylanase in terms of digestibility of dry matter (DM), crude protein (CP) and neutral detergent fibre (NDF). In vitro, the negative control had the lowest CP and NDF digestibility. Both DM and CP digestibility were increased (p<0.05) owing to xylanase supplementation either at 0.50 or 0.70 g/kg diet, and NDF digestibility was improved following xylanase addition at all of the test levels. There was a high linear correlation ($r^2>90$, p<0.05) between the activity concentration of the enzyme when transformed into its logarithmic value and in vitro digestibility coefficients of DM, CP or NDF. In the feeding trial, 112 crossbred pigs were randomly assigned to seven dietary treatments with 16 replicate pens of one pig each. An obvious dose effect on growth rate was observed ($r^2=0.79$, p<0.05) within the inclusion levels of xylanase. Compared with the negative control, xylanase addition at 0.70 g/kg diet resulted in significantly increased ADG (878 g/d vs. 828 g/d, p<0.05), and a tendency towards improved growth rate (868 g/d vs. 828 g/d, p = 0.10) was also observed following the inclusion of xylanase at 0.50 g/kg diet. It would appear that the nutrient utilization of corn and Chinese DLRM diets by pigs could be enhanced by an appropriate amount of xylanase addition. The in vitro and in vivo results suggested that the in vitro incubation method is feasible for predicting responses of pigs to exogenous enzymes and identifying those preparations that possess potential for improvement of the nutritive values of feedstuffs.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.6
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• pp.799-807
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• 2004

The objective of this field experiment was to investigate the growth, hay yield and chemical composition of cassava and stylo 184 grown under intercropping. The experiment was arranged in a Randomized Complete Block (RCB) design with 5 treatments and 4 replications. The treatments were: sole crop cassava (C); sole crop stylo 184 (S) and three intercropping treatments comprising an additive series of one (SC), two (SSC) and three (SSSC) rows of stylo 184 to one row of cassava. The results showed that leaf area per plant (LA) of cassava was significantly higher (p<0.05) in the sole crop relative to the intercropping treatments. Both total hay yield and CPDM yield were significantly higher (p<0.05) for C treatment and lower (p<0.05) for SSSC treatment. The total hay yield and CPDM yield were significantly greater (p<0.05) in the sole crop relative to the intercropping treatments. At the first and second harvests, CP content was similar among treatments; while at third and fourth harvests, CP contents were significantly greater (p<0.05) for the intercropping treatment relative to the sole crop. At the first and second harvest, NDF contents were significantly greater (p<0.05) in the sole crop relative to the intercropping treatments, whereas NDF contents were similar among intercropping treatments. Leaf area of stylo 184 at first and second harvest were significantly greater (p<0.05) for C, SC and SSC as compared with the SSSC treatments. At each harvesting, there were no significant differences in ash, CP, NDF, ADF and ADL contents of stylo 184 hay between the sole crop and intercropping treatments, except for the first harvest. ADF contents were significantly greater (p<0.05) in S, SC treatments relative to SSC and SSSC treatments. Both collective hay yield and CPDM yield of cassava and stylo 184 were significantly greater (p<0.05) for the SSC treatment and significantly lower (p<0.05) for the S treatment. Collective hay yield and CPDM yield were significantly greater (p<0.05) for the intercropping treatments relative to the sole crop. Based on this research, it was concluded that stylo 184 showed potential for intercropping with cassava. Intercropping cassava with stylo 184 has beneficial effects and can improve foliage biomass yield and soil fertility, which would be a more sustainable system than growing the cassava as a pure stand. In terms of hay yield and CP production, two rows of stylo 184 to one row of cassava could be the optimal pattern for this intercropping system.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.7
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• pp.1096-1103
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• 1999

A study was conducted to clarify the impact of recombinant porcine somatotropin (pST) on the efficiency of absorbed nitrogen use for protein deposition in growing pigs. Three levels of dietary crude protein (9.0, 11.5, 14.0% CP) were used. Each had either a sub-optimum or near optimum lysine: CP concentration (Low-lysine, 3.8 g/100 g CP and High-lysine, 5.5 g/100g CP) in order to achieve different metabolic efficiencies for nitrogen deposition (ca. 45 vs. 60%). Twelve crossbred female pigs $(59{\pm}4kg\;BW)$ were placed in metabolism cages and fitted with bladder catheters. Each pig received an excipient injection daily for the first 10-d, a pST (5 mg/d) injection for the second 10-d, and then excipient for the last 10-d. Pigs were randomly assigned to one of six dietary treatments (2 pigs/diet) and fed 4 times per d at $92g/kg\;BW^{0.75}$ $(3{\times}maintenance)$. Means for the excipient period were compared to means for the pST period. Urinary nitrogen (N) output declined in pST-treated pigs (p<0.01) irrespective of dietary protein content or lysine level. Nitrogen retention increased by an average of 11% (p<0.01) with pST treatment (726 vs. $803mg\;N/kg^{0.75}\;BW/d$). Forty-eight percent of the absorbed N was retained with Low-lysine diets, but this increased to 53% with pST injection (+11%, p<0.01). Pigs fed High-lysine diets retained 62% of absorbed N which increased to 69% with pST (+11% p<0.01). the addition of lysine improved N use by 27% (High vs. Low, p<0.01), but the effect of lysine and pST was additive (+40%). Therefore, pST improves N retention and the efficiency of apparently absorbed N use in growing pigs (>60kg). It does so with diets having the potential for either low or high efficiencies of N use (48% and 62%). More work is needed to determine if the partial efficiency of N use improves in direct proportion to pST dose since the improvement in protein deposition is a function of pST dose.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.73-73
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• 2017

To set an optimal harvest time of main 7 whole crop silage rice cultivars, Nokyang(Ny), Mogwoo(Mw), Mogyang(My), Jungmo1029(Jm1029), Jungmo1038(Jm1038), Nokwoo(Nw) and Yeongwoo(Yw), based on feed value, we analyzed their feed values such as percent crude protein(CP), crude fat(CF), crude ash(CA), neutral detergent fiber(NDF), acid detergent fiber(ADF) and lignin. It was evaluated every 10 days from heading to 40 days after heading(DAH). Total digestible nutrient(TDN) and relative feed value(RFV) was also calculated from ADF and NDF. As results, CP was generally in decline as increasing DAH in Ny, My, Jm1029, Jm1038 and Yw and decreased to 20 DAH in Mw, decreased after increasing to 30 days in Nw. The CP content of Ny was relatively the highest ranged from 5.3% to 10.1% and Mw the lowest 4.5% to 5.2%, compared to others. CF content tend to decrease as DAH increase in Ny, My and Nw and decreased after increasing to 30 days Jm1029, increased after decreasing to 20 days Jm1038 but was not shown distinct trend of increase or decrease in Mw. Especially, that of Yw's CF gradually increased as harvest time late but relative content the lowest from 1.46% to 2.29% among 7 cultivars. The CA content of Ny, My, Jm1029 and Jm1038 approximately decreased as DAH increased and that of Mw was similar to others after heading, Nw decreased after 10 days and Yw increased after flowering. In all 7 cultivars, NDF and ADF had a tendency to decrease as days accumulated, in particular, Yw was the lowest on 30 DAH and so the content of Yw's TDN the highest(71.5%), while the lowest(67.2%) in Nw. For lignin, particularly, Mw tend to be in decline as DAH increase and was the lowest ranged from 1.34% to 1.87%. ADF analyses allows for the evaluation of in vivo digestible dry matter(DDM) and energy availability and NDF analyses provides the best indication of dry matter intake(DMI). Ultimately, the two factors can be combined to derive RFV for forage. RFV in general increased as DAH increased in all cultivars and was in order, Yw>My>Ny> Jm1038>Mw>Jm1029>Nw on 30 DAH. Taken together when these results, despite a slight increase of TDN after 30 DAH except Yw, considering forage yield potential, digestibility of grains and gradual decrease of CP and CF, the yellow ripe stage, about 30 DAH, was appropriate to harvest whole crop rice for silage use.