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Identification of Secretion Signals of Target Proteins in Salmonella enterica serovar Typhimurium and Construction of Secretion Vector using this Signal (Salmonella enterica serovar Typhimurium에서 Type III 분비장치의 표적단백질들의 분비신호 확인 및 Type III 분비장치를 이용한 Secretion Vector의 개발)

  • Choi, Hyuk-Jin;Eom, Joon-Ho;Cho, Jung-Ah;Lee, Sun;Lee, Kyoung-Mi;Lee, In-Soo;Park, Yong-Keun
    • Korean Journal of Microbiology
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    • v.36 no.4
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    • pp.254-258
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    • 2000
  • Invasion process of bacterial cell into intestinal epithelium is important in Salmonella infection. The invasion is induced by the proteins secreted by type III secretion appratus of Salmonella. It has been known that the proteins do not have N-terminal signal peptide existing in general secreted proteins. Recent studies on Yersinia reported that secretion signal of type III appratus may lie on 5'end secondary structure of mRNA of secreted protein. In this study, we constructed translational fusion of ompR and sopE, encoding type III secretion protein of Salmonella, and observed secretion of the fusion protein for investigating the secretion signal of Salmonella type III appratus. The sopE DNA fragments of the translational fusion contain the region of promoter and from start code to tenth or to fifth code. These translational fusions indicate that type III secretion signal of Salmonella is located on 5'end of mRNA encoding secreted protein. We constructed prototype of secretion vector using this signal to produce useful foreign protein.

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Experimental and numerical simulating of the crack separation on the tensile strength of concrete

  • Sarfarazi, Vahab;Haeri, Hadi;Shemirani, Alireza Bagher;Zhu, Zheming;Marji, Mohammad Fatehi
    • Structural Engineering and Mechanics
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    • v.66 no.5
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    • pp.569-582
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    • 2018
  • Effects of crack separation, bridge area, on the tensile behaviour of concrete are studied experimentally and numerically through the Brazilian tensile test. The physical data obtained from the Brazilian tests are used to calibrate the two-dimensional particle flow code based on discrete element method (DEM). Then some specially designed Brazilian disc specimens containing two parallel cracks are used to perform the physical tests in the laboratory and numerically simulated to make the suitable numerical models to be tested. The experimental and numerical results of the Brazilian disc specimens are compared to conclude the validity and applicability of these models used in this research. Validation of the simulated models can be easily checked with the results of Brazilian tests performed on non-persistent cracked physical models. The Brazilian discs used in this work have a diameter of 54 mm and contain two parallel centred cracks ($90^{\circ}$ to the horizontal) loaded indirectly under the compressive line loading. The lengths of cracks are considered as; 10 mm, 20 mm, 30 mm and 40 mm, respectively. The visually observed failure process gained through numerical Brazilian tests are found to be very similar to those obtained through the experimental tests. The fracture patterns demonstrated by DEM simulations are mostly affected by the crack separation but the tensile strength of bridge area is related to the fracture pattern and failure mechanism of the testing samples. It has also been shown that when the crack lengths are less than 30 mm, the tensile cracks may initiate from the cracks tips and propagate parallel to loading direction till coalesce with the other cracks tips while when the cracks lengths are more than 30 mm, these tensile cracks may propagate through the intact concrete itself rather than that of the bridge area.

Characteristics of Temperature in Reformer Tube and Chemical Reaction for Steam Methane Ratio (수증기-메탄 혼합비에 따른 개질 튜브 내 온도 및 화학반응 특성)

  • Han, Jun Hee;Kim, Ji Yoon;Lee, Seong Hyuk
    • Journal of the Korean Institute of Gas
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    • v.20 no.5
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    • pp.27-33
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    • 2016
  • The aim of numerical study is the investigation of the solid and fluid temperatures in a reformer tube and chemical reaction characteristics of different steam-carbon ratio. We considered conjugate heat transfer contain radiation, convection and conductive heat transfers. This is because steam reforming reaction of hydrocarbon occurred high temperature conditions up to 800 K- 1000 K by using commercial computational fluid dynamics (CFD) code (Fluent ver. 13.0). For numerical simulation, the Reynolds-Averaged Navier-Stokes, momentum and energy equation were employed. In addition, inside of reformer tube is assumed as the porous medium to consider the Nichrome-based catalyst. To analysis characteristics of tube temperature in chemical reaction, we changed steam-methane ratio(SCR) from 1 to 6. As increased SCR, the higher tube temperature and methane conversion were observed. It was obtained that the highest hydrogen production held in SCR of 5.

Case Study of Building Special-Purpose Simple Browser (단순 웹브라우저의 설계와 구현 사례)

  • 우원택
    • Journal of Korea Society of Industrial Information Systems
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    • v.7 no.3
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    • pp.43-53
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    • 2002
  • The purpose of this study is to build a special -purpose browser using the powerful Microsoft web browser control so that we will be able to code and create a special-purpose browser. For this development, some literature surveys were reviewed to understand how the web browser looks like and is operated. To create our own web browser, we have designed a sample case and coded in Visual Basic. The primary task of this job was accomplished mainly with a form. It is made up with a bunch of image controls, a status bar on the button, a picture box to contain the image buttons, the combo box, the command button, the menus, the statements shown in the following exhibits and tables of the main text respectively. After combining them all together, we have tested the form to see if it works properly.'rho program was also enhanced to have a special purpose feature that permits the filtering function. The result was somewhat successful. However, it had some limitations such as the simplicity of the programming structures and functions. Overall, this study was useful for understand a general outline of special-purpose web browser development.

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Effect of a Time Dependent Concrete Modulus of Elasticity on Prestress Losses in Bridge Girders

  • Singh, Brahama P.;Yazdani, Nur;Ramirez, Guillermo
    • International Journal of Concrete Structures and Materials
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    • v.7 no.3
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    • pp.183-191
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    • 2013
  • Prestress losses assumed for bridge girder design and deflection analyses are dependent on the concrete modulus of elasticity (MOE). Most design specifications, such as the American Association of State Highways and Transportation Officials (AASHTO) bridge specifications, contain a constant value for the MOE based on the unit weight of concrete and the concrete compressive strength at 28 days. It has been shown in the past that that the concrete MOE varies with the age of concrete. The purpose of this study was to evaluate the effect of a time-dependent and variable MOE on the prestress losses assumed for bridge girder design. For this purpose, three different variable MOE models from the literature were investigated: Dischinger (Der Bauingenieur 47/48(20):563-572, 1939a; Der Bauingenieur 5/6(20):53-63, 1939b; Der Bauingenieur, 21/22(20):286-437, 1939c), American Concrete Institute (ACI) 209 (Tech. Rep. ACI 209R-92, 1992) and CEB-FIP (CEB-FIP Model Code, 2010). A typical bridge layout for the Dallas, Texas, USA, area was assumed herein. A prestressed concrete beam design and analysis program from the Texas Department of Transportation (TxDOT) was utilized to determine the prestress losses. The values of the time dependent MOE and also specific prestress losses from each model were compared. The MOE predictions based on the ACI and the CEB-FIP models were close to each other; in long-term, they approach the constant AASHTO value. Dischinger's model provides for higher MOE values. The elastic shortening and the long term losses from the variable MOE models are lower than that using a constant MOE up to deck casting time. In long term, the variable MOE-based losses approach that from the constant MOE predictions. The Dischinger model would result in more conservative girder design while the ACI and the CEB-FIP models would result in designs more consistent with the AASHTO approach.

Classification of Viruses Based on the Amino Acid Sequences of Viral Polymerases (바이러스 핵산중합효소의 아미노산 서열에 의한 바이러스 분류)

  • Nam, Ji-Hyun;Lee, Dong-Hun;Lee, Keon-Myung;Lee, Chan-Hee
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.285-291
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    • 2007
  • According to the Baltimore Scheme, viruses are classified into 6 main classes based on their replication and coding strategies. Except for some small DNA viruses, most viruses code for their own polymerases: DNA-dependent DNA, RNA-dependent RNA and RNA-dependent DNA polymerases, all of which contain 4 common motifs. We undertook a phylogenetic study to establish the relationship between the Baltimore Scheme and viral polymerases. Amino acid sequence data sets of viral polymerases were taken from NCBI GenBank, and a multiple alignment was performed with CLUSTAL X program. Phylogenetic trees of viral polymerases constructed from the distance matrices were generally consistent with Baltimore Scheme with some minor exceptions. Interestingly, negative RNA viruses (Class V) could be further divided into 2 subgroups with segmented and non-segmented genomes. Thus, Baltimore Scheme for viral taxonomy could be supported by phylogenetic analysis based on the amino acid sequences of viral polymerases.

Usage Techniques of a Truncated Message Authentication Code for In-Vehicle Controller Area Network (자동차 내부 네트워크를 위한 경량 메시지 인증 코드 사용기법)

  • Woo, Samuel;Lee, Sang-Bum
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.17 no.6
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    • pp.127-135
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    • 2017
  • Recently, the most brand new vehicles contain a lot of ECU for comfortable and safety driving environments. For efficient communication network among ECUs, almost car manufactures use CAN protocol which enables to decrease the number of communication lines dramatically and ensures higher data transmission reliability. However, CAN dose not ensure authentication of CAN data frame. So it is vulnerable to replay-attack on CAN data frame. This paper proposes the practical message authentication technique for In-vehicle CAN. To transmit data and MAC together, it is very useful to use the short length of MAC after considering limited space of CAN data frame. However to ensure safety of MAC, additional technique is required. We suggested a message authentication technique that can be usefully applied to build a safety network inside the vehicle because it considers limited data payload of CAN.

Comparison of WABA and Gd Burnable Absorbers Nuclear Characteristics and Optimal Allocation of Gd Rods in Fuel Assembly (WABA및 가도리니움 독봉 집합체에 대한 핵특성 비교 및 집합체내 가도리니아봉 위치 최적 선정)

  • Jung, Byung-Ryul;Yi, Yu-Han;Lee, Un-Chul;Park, Chan-Oh
    • Nuclear Engineering and Technology
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    • v.23 no.3
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    • pp.352-362
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    • 1991
  • Recent popular trends in pressurized water reactor(PWR) fuel management are to extend the cycle length and to employ the low-leakage core designs for the optimal utilization of the uranium resources. In control strategy incorporated with the fuel management, turnable absorbers are required to control the power peaking and to ensure a negative moderator temperature coefficient during reactor operation. In this study, the nuclear characteristics and the optimal allocation of gadolinium-poisoned rods within the fuel assembly are considered using KWU SAV 79 A Code Package. First, analyses are carried out to compare the nuclear characteristics of the fuel assemblies contain-ing WABA(Wet Annular Burnable Absorber) and Gadolinium burnable absorbers respectively. The analyses show that the gadolinium-bearing fuel assembly has peculiar depletion characteristics ensuing from the very large thermal neutron absorption cross section. Peculiar characteristics of gadolinium provide basis for the optimal allocation of Gd rods in fuel assembly. Second, the methodology of an optimal allocation of gadolinium-poisoned rods within the fuel assembly is developed and applied to some nuclear designs.

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Issues on Application between Letters of Credit Provisions of the UCC and the UCP (미국(美國) 통일상법전(統一商法典)의 신용장규정(信用狀規定)과 신용장통일규칙적용상(信用狀統一規則適用上)의 주요(主要) 쟁점(爭點))

  • Kang, Won-Jin
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.12
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    • pp.405-427
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    • 1999
  • Although Uniform Customs and practice for Documentary(UCP) is not a law, it applies to most documentary credits and is binding on all parties unless otherwise expressly stipulated. Besides, Uniform Commercial Code(UCC) Article 5 was codified by the United States and was adopted by every state. Moreover, the New York version of the UCC Article 5-102(4) specifically providing that the UCC does not apply to letters of credit where the parties agree to be governed by the UCP. Identical nonuniform Articles were latter added in Alabama, Arizona, and Missouri. The fact that courts in forty-six of the fifty states are bound by Article 5. Until now, Article 5 of the UCC has probably had an impact on the decisions in New York and the New York common law. Therefore, I examined a few issues on application between Article 5 of the UCC and the UCP. First, although the UCP attempt to introduce a new for examination of document by incorporating "standard practice of financial institutions" and "international banking practice", the standards for documentary compliance are not clear. The UCC attempt to rely on the matter of interpretation for the court, but the UCP would probably be interested in examining in about bank's internal practices as reflected in UCP Articles. Second, the rule for nondocumentary conditions is a useful for stand-by credit transactions under the UCC, but these conditions would probably put the bank in an even worse position in case of documentary credit transactions under the UCP. Third, the UCP does not contain any provision governing the fraud exception, but the UCC codified the fraud and forgery rules developed through American case law. Fourth, the UCP treats the issue of transfer in much more detail than the UCC does. In contract, the UCP's treatment of assignment of proceeds is brief. Finally, I suggest that the fraud exception rules should be prescribed in the UCP in order to protect the issuing bank and the applicant when an unscrupulous party attempts to defraud.

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An Iterative Soft-Decision Decoding Algorithm of Block Codes Using Reliability Values (신뢰도 값을 이용한 블록 부호의 반복적 연판정 복호 알고리즘)

  • Shim, Yong-Geol
    • The KIPS Transactions:PartC
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    • v.11C no.1
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    • pp.75-80
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    • 2004
  • An iterative soft-decision decoding algorithm of block codes is proposed. With careful examinations of the first hard-decision decoding result, the candidate codewords are efficiently searched for. An approach to reducing decoding complexity and lowering error probability is to select a small number of candidate codewords. With high probability, we include the codewords which are at the short distance from the received signal. The decoder then computes the distance to each of the candidate codewords and selects the codeword which is the closest. We can search for the candidate codewords which make the error patterns contain the bits with small reliability values. Also, we can reduce the cases that we select the same candidate codeword already searched for. Computer simulation results are presented for (23,12) Golay code. They show that decoding complexity is considerably reduced and the block error probability is lowered.