• Biomolecules & Therapeutics
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• 제23권1호
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• pp.26-30
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• 2015

Wnt/${\beta}$-catenin signaling pathway was mutated in about 90% of the sporadic and hereditary colorectal cancers. The abnormally activated ${\beta}$-catenin increases the cancer cell proliferation, differentiation and metastasis through increasing the expression of its oncogenic target genes. In this study, we identified an inhibitor of ${\beta}$-catenin dependent Wnt pathway from rhizomes of Atractylodes macrocephala Koidzumi (Compositae). The active compound was purified by activity-guided purification and the structure was identified as 2,8-dimethyl-6-hydroxy-2-(4-methyl-3-pentenyl)-2H-chromene (atractylochromene, AC). AC suppressed b-catenin/Tcell factor transcriptional activity of HEK-293 reporter cells when they were stimulated by Wnt3a or inhibitor of glycogen synthase kinase-$3{\beta}$. AC down-regulated the nuclear level of ${\beta}$-catenin through the suppression of galectin-3 mediated nuclear translocation of ${\beta}$-catenin in SW-480 colon cancer cells. Furthermore, AC inhibits proliferation of colon cancer cell. Taken together, AC from A. macrocephala might be a potential chemotherapeutic agent for the prevention and treatment of human colon cancer.

• 농업과학연구
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• 제41권4호
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• pp.303-308
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• 2014

This study was conducted in order to utilize the basic data for weed control by surveying the occurrence of weed species. Total 63 sites of upland Chinese cabbage fields in Chungnam Provinces were investigated. The result of survey, 71 weed species in 25 families were identified and classified to 39 annuals, 16 biennials and 16 perennials. Based on the occurrence ratio, the most weed species belonged to Compositae (20 species). 8, 6 and 5 weed species belonged to Poaceae, Cruciferae and Polygonaceae, respectively, and these 10 weed species in the most six families accounted for 50% of total weed occurrence. The most dominant weed species in upland Chinese cabbage fields were Portulaca oleracea (8.07%), followed by Digitaria ciliaris (7.54%), Rorippa palustris (6.44%), Chenopodium album (5.73%), Echinochloa crus-galli (5.02%) and Cyperus amuricus (3.95).

• 한국환경과학회지
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• 제27권10호
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• pp.907-923
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• 2018

The aim of this study was to determine the appropriate stockpiling methods for revegetation by comparing the germination status of seed banks before and after preservation for 2 years. Soil temperature in stockpiled topsoil was higher in open treatment and at 1.5 m, whereas soil water content was maintained at lower levels (14.06-19.08%), than those in the control group. The seed banks in stockpiled topsoil had 48 species and 1,559 individuals, among which perennials showed the highest number in terms of life forms, whereas Compositae and Gramineae were dominant in terms of families. Based on seed bank type, persistent seed banks had the highest number of species, while transient seed banks had the highest number of individuals. By stockpiling period, the number of species in the seed bank started to increase after 24 months, while the number of individuals began increasing after 12 months and exceeded that of the control group after 24 months. Regarding the treatment of stockpiling methods, the number of species and individuals in open treatment were closer to those of the control group. When analyzed by height, the number of species and individuals were higher at 0 m, but still lower than those of the control group. A multivariate analysis of variance (MANOVA ) showed that the optimal combination was obtained in open treatment and the number of individuals increased with the lengthening of the stockpiling period.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.102.1-102
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• 2003

A bacterial strain YC4963 with antifungal activity against Colletotrichum orbiculare, a causal organism of cucumber anthracnose was isolated from the rhizosphere soil of Siegesbeckia pubescens (Siegesbeckia pubescens Makino；Family：Compositae) in Korea. Based on physiological and biochemical characteristics and 16S ribosomal DNA sequence analysis, the bacterial strain was identified as Pseudomonu aureofaciens. The bacteria also inhibited mycelial growth of several plant fungal pathogens such as Botrytis cinerea, Fusarium oxysporum and Rhizoctonia solani on PDA and 0.1 TSA media. The antibiotic activity was found from the culture filtrate of TSB(tryptic soy broth) and its active compounds were quantitatively bound to XAD adsorber resin. The antibiotic spectrum was broad and growth of C. orbiculare and F. oxysporum, B. cinerea were inhibited at very low concentration. The chemical data from various chromatographic procedures showed that active fraction consisted of at least two phenazine derivatives. However, the metabolites had no inhibitory effect on Pythium ultimum which was reported to be sensitive to phenazine antibiotics. The compounds responsible for the activity are now under investigation.

• 한국약용작물학회지
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• 제13권5호
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• pp.284-287
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• 2005

The flowers of Chrysanthemum boreale Makino were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with n-hexane, EtOAc, n-BuOH and $H_2O$. Two compounds from the n-hexane fraction and one glucoside from the n-BuOH fraction were isolated through the repeated silica gel and ODS column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as ${\beta}-sitosterol$ (1), phytol (2) and zingerone $4-O-{\beta}-D-glucopyranoside$ (3). Compounds 2 and 3 were isolated for the first time from this plant.

• Journal of Ecology and Environment
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• 제31권3호
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• pp.225-232
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• 2008

To find out the status and characteristics of exotic plants in the Gwangreung Forest, a representative forest ecosystem in central Korea, we surveyed the species composition, coverage, and habitat illuminance of exotic plant species in a set of quadrats established along roads and trails in and around the Gwangreung Forest and buffer zone areas, In 1932, only five species of exotic plants were found along paths and roads in the Gwangreung Forest. However, the number of species in the forest has dramatically increased since 2000, when Ambrosia artemisiifolia L. and A. trifida L. were first recorded, and in 2007, 38 species of exotic plants were recorded. Among the 11 families, 23 genera and 25 species of exotic plants recorded in the quadrats, Compositae was the most common family (11 species), and perennial herbs, 42%, were the most frequently occurring life type, followed by annual herbs (31%) and biennial herbs (19%). Plants of North American origin comprised 48% of exotic species identified in our surveys. Exotics were found most frequently in quadrats along roads in the forest, followed by the buffer zones and hiking trails. The number of species and individuals of exotic plants decreased as we moved deeper into the forest, but Aster pilosus Willd. and Erigeron annuus (L.) Pers. were identified along hiking trails in the interior, and appeared to be capable of spreading further and more rapidly into the forest than other species.

• 생약학회지
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• 제25권3호
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• pp.209-213
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• 1994

For the investigation of medicinal resources from Taraxacum species, the studies were carried out to evaluate the pharmaco-constituents in the aerial part of Taroxacum hallaisanensis, an endemic plant of Korea. From BuOH fraction of the MeOH extract, compound 1 (protocatechuic acid, $C_7H_6O_4,\;3,4-dihydroxy\;benzoic\;acid)$, compound 2 $[C_{22}H_{31}O_6,\;luteolin-7-O-{\alpha}-_L-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-_D-glucopyranoside]$, and compound 3 $[C_{15}H_{20}O_6,\;luteolin-7-O-{\beta}-_D-glucopyranoside]$ were isolated by column chromatographic separation using polyamide and ODS-gel. The structures were elucidated by means of physico-chemical evidences($^1H-NMR,\;{12}^C-NMR$, IR, EI-Mass, FAB-Mass and GC).

• Journal of Plant Biology
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• 제39권2호
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• pp.149-159
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• 1996

The order of Rhododendro-Quercetalia mongolicae representing temperate oak forests in Korea was characterized in terms of presence of taxa of different rank. 140 releves were analysed for percentage contribution of each species, genera and families in syntaxa under consideration as well as extracton of diagnostic genera and families for syntaxa by an ordination technique. The Rhododendro-Quercetalia is characterized by high diversity of tree and shrub species contributing 40% of the total floristic composition as well as by a high contribution of the genus Rhododendron and the absence of the genus Fagus, characteristic of the QuercoFagetea sensu lato. The character families for the Rhododendron and Quercus include Liliaceae and Compositae, whereas Acer, Carex, Viola, Rhododendron and Quercus are the most common among genera. Rhododendron and Quercus are regarded as the transgressive cheractergenera, whereas the families of Pinaceae and Ericaceae are considered companions (in sense of the terminology of the Braun-Blanquet syntaxonomy) for the order. Family appeared to be an inadequate rank for diagnoses of alliances and suballiances. On the other hand, genus was found to be the most effective rank in differentiating the alliances and suballiances. The Lindero-Quercenion shares the same character-genera with the order Rhododendro-Quercetalia. Character genera of the suballiances Callicarpo-quercenion are Carpinus, Styrax, Smilax and Callicarpa, and those of the Pino-Quercion list Euonymus, Saussurea and Tilia.

• 한국자원식물학회지
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• 제8권2호
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• pp.143-151
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• 1995

The development of medicinal plants for cut plants and landscape plants were conducted through the literiture survey. The results were as follows: We selected 38 species of medicinal plants including 7 medicinal woody plants for cut plant and landscape plants from 450 medicinal plants. There were 8 species of Compositae, 4 species Ranunculaceae, 3 species Rosaceae and Umbelliferae, 2 species Polygonaceae and Campanulaceae respectively. The anthesis of Cornus officinalis, Magnolia denudata, Forsythia koreana of medicinal woody plants flowering before leaf spreading come to early than the anthesis of other medicinal plants. There were 4 species(11%) over 10cm of the flower diameter, 6 species(16%) over 5cm below 10cm, 19 species(50%) over 1cm below 5cm and 9 species(24%) 1cm below. In terms of flower color, 11 species(29%) were white, 9 species(24%) yellow, 6 pecies(16%) red, 5 species(13%) violet and 3 species(8%) were blue aided violet. In terms of the flowering by month, 12 species(32%) on July, 9 species(24%) on May, 6 species(16%) on June and August respectively, and 3 species(8%) on March. The most medicinal plants were propagated by division and seeding easily, but woody medicinal plants of 7 species of this study were propagated by cutting and grafting easily. The percentage of usage of parts of medicinal plants including woody medicinal plants was that roots were 20 species(53%), flowers 6 species(16%), fruits and all part of medicinal plants were 4 species(11%), and seeds and stem including leaves 2 species(5%) respectively.

• 생약학회지
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• 제38권2호통권149호
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• pp.181-186
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• 2007

Seven phenolic compounds, scopoletin (1), caffeic acid methyl ester (2), apigenin 7-O-${\alpha}$-L-rhamnosyl-(1${\rightarrow}$6)-O-${\alpha}$-D-glucoside (3), isorhamnetin 7-O-${\alpha}$-D-glucoside (4), isorhamnetin 3-O-${\alpha}$-D-glucoside (5), luteolin (6), and quercetin 3-methyl ether (7) were isolated from the methanol extract of the flowers of S. excelsus. Their structures were established by chemical and spectroscopic methods. The isolated compounds were tested for their cytotoxicity against four human cancer cell lines in vitro using a SRB method. The compounds 4 and 7 showed moderate cytotoxicity with ED$_{50}$ values ranging from 1.59 to 13.14${\mu}$g/ml.