• 아동학회지
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• 제18권1호
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• pp.5-21
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• 1997

This study compared children's daily activities In the preschools of middle-and lower-income communities. 22 children, evenly divided by community and children's gender, were observed for 3 hours on 5 consecutive days. The observers followed the target child, gathering data during 30-second "windows" every 4 1/2 minutes. A total of 750 observations were used in the analyses. The findings show the variation in children's activities as a function of community differences. In the case of academic activities, children of middle-income community were more exposed to and engaged in play with academic objects more than children of the of low-income community. A reverse tendency, however, was found in academic lessons. Children of low-income community were more exposed to work than their counterparts. Children of middle-income community were engaged in child-adult conversation more than those of low-income community. In addition, children of the middle-income community initiated their involvement in play and conversation more than their counterparts, though no difference was found in children's initiation of activities.

• 미생물학회지
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• 제55권3호
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• pp.220-225
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• 2019

최근 10년간 미생물생태분석 기반의 연구는 차세대염기서열분석법이 개발된 이래로 지속적으로 증가하고 있다. 장내미생물생태와 건강의 연관성은 미생물 생태학 분야에 있어서 중요한 결과로 여겨지고 있다. 미생물 군집 분석은 주로 16S rRNA 유전자 가변 영역의 염기서열을 통해 분석되지만 이는 미생물의 활성 정보를 제공하지 않는다. 본 연구에서는 cDNA 기반의 미생물 생태분석을 수행하고 DNA 및 cDNA기반의 미생물생태분석 결과를 비교하였다. 두 가지의 서로 다른 접근법이 Butyrate producer와 probiotics와 같이 장내 대사과정에서 중요한 미생물의 abundance 뿐만 아니라 비만 지표로 알려진 Firmicutes 와 Bacteroidetes의 비율에 있어서 차이가 있음을 나타내었다. 따라서, cDNA 기반 미생물 군집은 이전에 수행된 DNA 기반 미생물 군집 분석과 비교하여 장내미생물생태의 역할과 관련된 또 다른 분석 방향성을 제공한다.

• Mycobiology
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• 제50권1호
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• pp.55-65
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• 2022

Lichen is a symbiotic mutualism of mycobiont and photobiont that harbors diverse organisms including endolichenic fungi (ELF). Despite the taxonomic and ecological significance of ELF, no comparative investigation of an ELF community involving isolation of a pure culture and high-throughput sequencing has been conducted. Thus, we analyzed the ELF community in Parmotrema tinctorum by culture and metabarcoding. Alpha diversity of the ELF community was notably greater in metabarcoding than in culture-based analysis. Taxonomic proportions of the ELF community estimated by metabarcoding and by culture analyses showed remarkable differences: Sordariomycetes was the most dominant fungal class in culture-based analysis, while Dothideomycetes was the most abundant in metabarcoding analysis. Thirty-seven operational taxonomic units (OTUs) were commonly observed by culture-and metabarcoding-based analyses but relative abundances differed: most of common OTUs were underrepresented in metabarcoding. The ELF community differed in lichen segments and thalli in metabarcoding analysis. Dissimilarity of ELF community intra lichen thallus increased with thallus segment distance; inter-thallus ELF community dissimilarity was significantly greater than intra-thallus ELF community dissimilarity. Finally, we tested how many fungal sequence reads would be needed to ELF diversity with relationship assays between numbers of lichen segments and saturation patterns of OTU richness and sample coverage. At least 6000 sequence reads per lichen thallus were sufficient for prediction of overall ELF community diversity and 50,000 reads per thallus were enough to observe rare taxa of ELF.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2127-2137
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• 2016

A mangrove microbial community was analyzed at the gene and protein levels using metagenomic and proteomic methods with the green macroalgae Enteromorpha prolifera as the substrate. Total DNA was sequenced on the Illumina HiSeq 2000 PE-100 platform. Two-dimensional gel electrophoresis in combination with liquid chromatography tandem mass spectrometry was used for proteomic analysis. The metagenomic data revealed that the orders Pseudomonadales, Rhizobiales, and Sphingomonadales were the most prevalent in the mangrove microbial community. By monitoring changes at the functional level, proteomic analyses detected ATP synthase and transporter proteins, which were expressed mainly by members of the phyla Proteobacteria and Bacteroidetes. Members of the phylum Proteobacteria expressed a high number of sugar transporters and demonstrated specialized and efficient digestion of various glycans. A few glycoside hydrolases were detected in members of the phylum Firmicutes, which appeared to be the main cellulose-degrading bacteria. This is the first report of multiple "omics" analysis of E. prolifera degradation. These results support the fact that key enzymes of glycoside hydrolase family were expressed in large quantities, indicating the high metabolic activity of the community.

• 보건교육건강증진학회지
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• 제33권5호
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• pp.107-119
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• 2016

Objectives: This study aims at examining the association of sense of community with community participation for health promotion in urban areas of Korea. Methods: We analyzed data from 'Community Capacity for Healthy Gangdong Communites' Survey' in 2007. The survey was based on self-reported questionnaires, which were distributed to 1,800 community residents over the age of nineteen in five administrative communities of Gangdong-gu, Seoul, in Korea by using proportionate probability sampling method. We measured 'Sense of community' with four indicators including 'Good neighborhoods', 'Perceived possibility of cooperation', 'Pride of community' and 'Possibility of development' by using 5-point Likert scales. Community participation was measured with the experience rate or the extent of participation by 5-point Likert scales in seven community actions or activities including voting, community program planning, social actions, etc. We examined the association of sense of community with community participation by using regression analyses. Results: This study has shown that sense of community was associated with and made positive impacts on community participation in diverse community actions or activities in urban communities. Conclusions: For promoting community health in urban areas, we can increase community participation more effectively with the efforts of enhancing sense of community.

• 대한지역사회영양학회지
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• 제11권3호
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• pp.383-391
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• 2006

The purposes of this study were to examine the impacts of characteristics of bakery cafe customers and service quality on customer perceived value, customer satisfaction, intention to revisit, and intention to recommend, and to provide comprehensive understanding on attitudes and behaviors of bakery cafe customers. The questionnaire was developed through literature review and focus group interviews and was modified after pilot test. The questionnaires for the main survey were distributed to 320 males and females aged 20 and over in Seoul. A total of 275 questionnaires were used for analyses (85.9%) and the statistical analyses were completed using SPSS Win (12.0) for descriptive analyses, regression analyses, and correlation analysis. The main results were as follows. There were significant differences of intention to revisit between groups of different gender, age, and personal expenses. The service quality of product dimension affected all tested variables, and the service quality of services and price dimension had effects on customer perceived value and intention to recommend. Customer satisfaction was a unique variable that is affected by service quality of the interior environments dimension. Overall, researchers and managers of bakery cafes should understand customer attitudes and behaviors, and the results of this study would help to establish marketing strategies that meet target customers' needs.

• 생태와환경
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• 제50권3호
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• pp.325-336
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• 2017

The ecosystem in the Gwangju Stream has taken a wide range of disturbance such as the discharging water of sewage treatment plant, the lake water and the river water from different water system over the past decade. This study was figured out some significant influence factors by analyzing the relationship between biotic and abiotic factors in the urban stream. Abiotic components included 15 water quality variables which were measured in five sampling sites along the stream from October 2014 to July 2015, whereas the benthic macroinvertebrates found in those sites were used to estimate various biotic indices representing the ecological status of the community. The results of correlation analyses indicated that abiotic factors by human activities affected on the inhabitation of benthic macroinvertebrates more than biotic factors. The results of cluster analyses and ANOVA tests also showed that biotic and abiotic characteristics were clearly different in season. The main influence factors of cluster analysis by sites were $NH_3-N$, EPT(I) and DO. It was considered that more various statistical analyses would be necessary to find some different relationships and influence factors between biotic and abiotic variables in the urban stream.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.21-29
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• 2010

The phenolic compounds are a major contaminant class often found in industrial wastewaters and the biological treatment is an alternative tool commonly employed for their removal. In this sense, monitoring microbial community dynamics is crucial for a successful wastewater treatment. This work aimed to monitor the structure and activity of the bacterial community during the operation of a laboratory-scale continuous submerged membrane bioreactor (SMBR), using PCR and RT-PCR followed by denaturing gradient gel electrophoresis (DGGE) and 16S rRNA libraries. Multivariate analyses carried out using DGGE profiles showed significant changes in the total and metabolically active dominant community members during the 4-week treatment period, explained mainly by phenol and ammonium input. Gene libraries were assembled using 16S rDNA and 16S rRNA PCR products from the fourth week of treatment. Sequencing and phylogenetic analyses of clones from the 16S rDNA library revealed a high diversity of taxa for the total bacterial community, with predominance of Thauera genus (ca. 50%). On the other hand, a lower diversity was found for metabolically active bacteria, which were mostly represented by members of Betaproteobacteria (Thauera and Comamonas), suggesting that these groups have a relevant role in the phenol degradation during the final phase of the SMBR operation.

• Journal of Applied Biological Chemistry
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• 제59권4호
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• pp.299-304
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• 2016

PCR 산물을 이용한 시퀀싱방법 중 Illumina 플랫폼으로 시퀀싱을 수행하면 100개 이상의 인위적인 시퀀스가 생겨나며, 그러한 인위적으로 형성되는 시퀀스에 의해 Operational taxonomic units를 기반으로 한 미생물생태 변화 및 네트워크 분석에 영향을 미친다. 이러한 문제점이 있음에도 불구하고 분변미생물생태를 분석하는데 Illumina에서 제공하고 있는 시퀀싱을 주된 방법으로 사용하고 있으며, 또한 그러한 시퀀스 기반의 분변미생물 생태분석 결과는 분변샘플상태(i.e., 분변 보관 기간, 분변양, 분변의 신선도)에 따라 상이하게 나타난다. 본 연구에서는 분변샘플의 동결건조가 시퀀스 데이터의 퀄리티를 향상시키는지 관해 조사하였으며, 이를 통해 분변샘플에 동결건조처리는 전체적인 미생물생태구조를 변화시키지는 않지만 인위적으로 형성되었을 가능성이 있는 시퀀스의 수를 감소시키는 것으로 확인되었다. 따라서, 분변으로부터 DNA를 추출하기 이전에 동결건조처리하는 방법을 Illumina 기반의 분변미생물생태분석에 사용하는 것을 권장한다.

• Educational Technology International
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• 제19권1호
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• pp.1-33
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• 2018

The present study aimed to develop a self-reported measurement instrument - the Empathetic School Community Competency Inventory (ESCCI)-to better understand members' empathetic sense to schools as their community in the context of secondary schools in South Korea. Based on a synthesis of the literature on the school community, empathy, and competencies, and a series of preliminary analyses with a panel of expert judges and pilot tests, initial ESCCI items were developed. In total, 435 students and 134 teachers from secondary schools in South Korea provided usable data as measured by the ESCCI. The results of EFA and CFA suggested a five-factor model: culture of respect ( α = .94), empathetic community identity ( α = .93), communication structure (α = .91), emotion immersion (α = .91), and caring process (α =.89) with χ² (980, n = 285) = 3080.169; p-value < .0001, RMSEA = 0.068; 90% CI [.059, .064], p-value < .0001; CFI = .88; SRMR = 0.04; and TLI = .88, leaving 46 items out of initially developed 76 items. The ESCCI model developed based on the findings of the study can be used to assess schools' competency as an empathetic community and design programs to promote empathetic school cultures in secondary schools in South Korea. Implications and limitations of the study are discussed.