• Tuberculosis and Respiratory Diseases
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• v.50 no.4
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• pp.450-461
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• 2001

Background : The underlying pathogenesis of radiation-induced lung fibrosis (RTLF) has not been very well defined. However, the role of TGF-$\beta$ in the generation of RTLF has been a major focus because there is an increase in the expression of both the TGF-${\beta}m$-RNA and its protein preceding RTLF lesions. The down stream signal after a TGF-$\beta$ stimulated lung fibrosis includes the activation of many mediators such as Smad and c-Jun N-terminal kinase (JNK) through TAK1. It is we hypothesized that JNK activation may play a pivotal role in RTLF pathogenesis through increased transcription of the fibrogenic cytokines. The present study evaluates JNK activity in alveolar macrophages after irradiation and the relationship between JNK activity and the amount of collagen in the lung tissues. Methods : C57BL/6 mice(20-25 gr, males) received chlorotetracycline(2g/L) in their drinking water 1 week prior to irradiation and continuously there after. The mice were irradiated once with 1400 cGy of $60CO{\gamma}$-ray over the whole chest. The cellular composition of the whole lung bronchoalveoalr lavage fluids(BALF), elastin expression in the lung tissues, the level of hydroxyproline in lung tissues, and an in vitro JNK assay was measured before irradiation and one, four, and eight weeks after irradiation (RT). Results : The volumes of BALF retrieved from instilled 4 mL of saline with 2% heparin were 3.7-3.8 mL for each group. The cell numbers were similar before($4.1{\times}10^4{\pm}0.5{\times}10^4/mL$) and 1 week($3.1{\times}10^4{\pm}0.5{\times}10^4/mL$) after RT. At four and eight weeks after RT, the cell number reached to $14.0{\times}10^4{\pm}1.5{\times}10^4mL$ and $10.0{\times}10^4{\pm}1.3{\times}10^4/mL$, respectively. There we no changes in the lymphocytes and neutrophils population observed in the BALF after RT. The H-E stain of the lung tissues did not show any structural and fibrotic change in the lung tissues at 4 and 8 weeks after RT. In addition, the amount of elastin and collagen were not different on Verhoeff staining of the lung tissues before RT to eight weeks after RT. The hydroxyproine content was measured with the left lung dissected from the left main bronchus. The lung were homogenized and hydrolyzed with 6 N Hel for 12 hours at $110^{\circ}C$ then measured as previously described. The content of hydroxyproline, standardized with a lung protein concentration, reached a peak 4 weeks after RT, and thereafter showed a plateau. AnIn vitro JNK assay using c-$Jun_{1-79}$-GST sepharose beads were performed with the alveolar macrophages obtained from the BAL. JNK activity was not detected prior to RT, However, the JNK activity increased from one week after RT and reached a peak four weeks after RT. Conclusion : JNK may be involved in the pathogenesis because the JNK activity showed similar pattern observed with the hydroxyproine content. However, it is necessary to clarify that the JNK increases the transcription of fibrogenic cyiokines through the transcription factor.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.6
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• pp.659-670
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• 2017

Oxidative stress and inflammation are key factors responsible for progression of liver injury. A variety of functions of oyster hydrolysate (OH) are affected by their antioxidant and anti-inflammatory activities. However, little is known regarding the effects of OH on a liver injury model. This study was performed to evaluate the effects of OH on acute liver injury induced by lipopolysaccharide/D-galactosamine (LPS/D-GalN) in mice. Experimental groups were divided into six groups as follows (each group, n=10): control (saline), LPS/D-GalN, LPS/D-GalN+OH (100 mg/kg), LPS/D-GalN+OH (200 mg/kg), LPS/D-GalN+OH (400 mg/kg), and LPS/D-GalN+silymarin (25 mg/kg, positive control). The experimental acute liver injury model was induced with LPS ($1{\mu}g/kg$) and D-GalN (400 mg/kg). We first analyzed antioxidant and anti-inflammatory activities in OH. OH showed high DPPH and ABTS radical scavenging activities and reduced ROS generation in Chang cells in a dose-dependent manner. In addition, OH showed anti-inflammatory activities, such as inhibition of cyclooxygenase-2 and 5-lipooxygenase. Treatment with OH down-regulated tumor necrosis factor $(TNF)-{\alpha}$, interleukin (IL)-6, and $IL-1{\alpha}$ expression levels in LPS-stimulated RAW264.7 cells. OH significantly reduced LPS/D-GalN-induced increases in the concentrations of alanine transaminase and aspartate aminotransferase in serum. In the LPS/D-GalN group, liver tissues exhibited apoptosis of hepatocytes with hemorrhages. These pathological alterations were ameliorated by OH treatment. Consistently, hepatic catalase activity was low in the LPS/D-GalN group compared to the control group, and catalase activity was significantly restored by OH treatment (P<0.05). Furthermore, OH markedly reduced the LPS/D-GalN-induced increase in $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6 levels in liver tissue. Taken together, these results show that OH has hepatoprotective effects on LPS/D-GalN-induced acute liver injury via inhibition of oxidative stress and inflammation, suggesting that OH could be used as a health functional food and potential therapeutic agent for acute liver injury.

• Korean Journal of Poultry Science
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• v.15 no.3
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• pp.211-218
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• 1988

Data on egg number from time of first lay to 71 week of age were obtained from samples of single comb white leghorn breeder and brown layer breeder populations which were raised at manny breeding Co. These data were used to estimate the heritabilities of age at sexual maturity, early egg number, residual egg number and annual egg number. Also, the genetic correlation coefficients between these traits were estimated and selection efficiencies of each segment in early part record were estimated by use of the heritabilities and generic correlations. The estimated heritabilities and standard errors in two lines(W and B) were $0.30\pm0.07$ and $0.33\pm0.08$ at early records, $0.19\pm0.06$ and $0.18\pm$0.05 at residual records and $0.37\pm0.09$ and $0.49\pm0.10$ at time of first lay. Those at annual records were $0.24\pm0.06$ equally. The estimated correlation coefficients and standard errors between early record and annual record were $0.76\pm0.08$ and $0.77\pm0.07$. Those between early record and residual record were $0.46\pm0.15$ and $0.39\pm0.16$ respectively. At improving annual egg production per year, selection based on early part record(SM to 40 week of age) would be 69% more efficient than on annual record in line W and 80% more efficient than on annual record in line B. But, if it were considered that egg weight decreased due to sexual maturity and cost for data collection, use of selected segment from 35~40week of age in line W and from 31~40 week of age in line B would be desired in spite of a. small loss in relative selection efficiency. The generation interval would not be shortened.

• Journal of the Korean Applied Science and Technology
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• v.29 no.3
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• pp.450-458
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• 2012

In order to reduce the effects of greenhouse gas (GHG) emissions, the South Korean government has announced a special platform of technologies as part of an effort to minimize global climate change. To further this effort, the Korean government has pledged to increase low-carbon and carbon neutral resources for biofuel derived from biomass to replace fossil and to decrease levels of carbon dioxide. In general, second generation biofuel produced form woody biomass is expected to be an effective avenue for reducing fossil fuel consumption and greenhouse gas (GHG) emissions in road transport. It is important that under the new Korean initiative, pilot scale studies evolve practices to produce biomass-to-liquid (BTL) fuel. This study reports the quality characteristics of F-T(Fischer-Tropsch) diesel for production of BTL fuel. Synthetic F-Tdiesel fuel can be used in automotive diesel engines, pure or blended with automotive diesel, due to its similar physical properties to diesel. F-T diesel fuel was synthesized by Fischer-Tropsch (F-T) process with syngas($H_2$/CO), Fe basedcatalyst in low temperature condition($240^{\circ}C$). Synthetic F-T diesel with diesel compositions after distillation process is consisted of $C_{12}{\sim}C_{23+}$ mixture as a kerosine, diesel compositions of n-paraffin and iso-paraffin compounds. Synthetic F-T diesel investigated a very high cetane number, low aromatic composition and sulfur free level compared to automotive diesel. Synthetic F-T diesel also show The wear scar of synthetic F-T diesel show poor lubricity due to low content of sulfur and aromatic compounds compared to automotive diesel.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.10 no.5
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• pp.209-217
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• 2015

Development of food industry and increased city life induced enlarged generation of food waste which is currently reused in a wide range of way. In this study, treatment of food waste generated from the public agricultural product wholesale markets (they are categorized as business places which imposes the duty for reducing the food waste discarded) in Korea was investigated, and subsequently, a scheme for improving the food waste recycling business was suggested. The food waste treatment plants are mainly located in Kyeongki-do at 39.5% of total plants in Korea and the other provinces have less than 10% of the total numbers, among which public treatment plant was 38.0% and private plant was 62.0%. The treatment methods included recycling as animal feed at 47.5%, as compost at 36.4%, and the rest of food waste (12.6%) was treated in other ways. Remarkably, it was noticed that the amount of food waste treated in anaerobic digestion have been increased up to 5.4% since 2011. This implied that food waste treated in anaerobic digestion method is gradually increased according to government policy. Amongst 33 public agricultural product wholesale markets distributed all over the country, the trading volume dealt in Garakdong market in Seoul, Gangseo market in Seoul, Daegu market in Daeju, Eomgung market in Busan, Guri market in Guri accounted for 34.67, 7.47, 6.98, 5.41, and 5.30% of the total trading volume in Korea, respectively. 2.65% of the total trading agricultural products dealt in the markets were remained as food waste and treated. In 2006, Ministry of Agriculture, Food and Rural Affairs implemented the package policy for radish and Chinese cabbage in order to reduce the amount of food waste and indeed, food waste generated in Garakdong market declined to one third of the food waste before the package policy implementation. In 2010, the food waste amount treated by 'dehydration', 'discarding as raw materials', and 'drying after dehydration' accounted for 56.3%, 33.7%, and 10.0% of the total food waste generated in the public agricultural product wholesale market. However, in 2013, discarding as raw materials accounted for the most at 56.3% followed by dehydration at 37.5%, and by drying after dehydration at 6.3%. The remarkable increment of the food waste discarded as raw materials was attributed to the increase of them in Garakdong market in Seoul. In general, the agricultural food waste contains high amount of moisture at 88% in average and low amount of salt at 0.02% in average. Therefore, it is highly recommended to treat the agricultural food waste through drying after dehydration in order for improving the treatment capability. Also, this recommendation can be supported by the fact that the end-products via drying after dehydration can be utilized as compost material. Overall, the agricultural food waste recycling business can be developed through integration of the treatment via 'drying after dehydration' and compost production.

• Journal of Korea Water Resources Association
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• v.42 no.2
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• pp.141-148
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• 2009

Each river in each country has peculiar channel characteristics at every section. Korea consists of mountains at the rate of 70%. Due to this, the sections that display unique channel form are scattered. One of the typical features is the narrow pass of channel section that presents in the process of the generation of alluvial channel and deformation channel. Around as this narrow pass of channel, it often comes to be regular flood disaster section. Regardless of this situation, investigations and plans that reflect channel characteristics at the narrow pass of channel have been wholly wanting until now. In accordance with this, we have investigated and analysed hydraulic significance and channel form of the narrow pass of channel in Seonsan in this study. The study has found real minute classification of sediment phenomenon at river bed of low flow channel on investigation section which is sand-bed river, but it was vaguely difficult to conclude that classification of sediment arises from explicit natural force. We were able to assume that the phenomenon comes from gentle bed slope, supply of river bed materials which is nearly uniformity. However, classification of sediment would come from collecting an aggregate rather than natural force. And we found that compound cross section conversion has been developing dramatically with channel form of movable bed section referring to movement which was active before at drainage district of the narrow pass of channel. With this, we could find the phenomenon that river width of low flow channel diminished largely. This kind of situation implies that it may cause compound section weighting, flood dimension decreasing, revetment at low flow channel or the local scour at bottom of levee when extraordinary flood occurs.

• Resources Recycling
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• v.32 no.3
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• pp.57-67
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• 2023

The treatment of waste plastic has primarily been entrusted to small companies, which has resulted in challenges in obtaining an accurate overview of the current state of affairs and ensuring profitability. Consequently, despite the presence of recycling technology, their practical application has proven to be challenging. In this study, as part of the waste plastic material recycling plan, it is assumed that the PET/OPP laminated waste film is peeled off at the waste film generation site for the second use. The recycling rate of PET/OPP delaminated waste film is assumed to be 2%, 10%, and 30% referring to the figures suggested by "Life-cycle Post Plastic Measures" from the Korean government. In this study, a physical separation method was developed as a recycling approach for waste PET. A result of cost-benefit analysis was conducted to evaluate the economic viability of the recycling process based on changes in the recycling rate. The findings indicated that a recycling rate of waste PET was 30% or higher resulted in a cost-benefit ratio (Benefit-cost ratio, BCR) of 1.32, exceeding the threshold of BCR ≥1, which is considered to meet the minimum requirement for cost-benefit balance. As the government's allocation ratio and unit price are expected to increase in the future, the cost-benefit ratio is expected to increase further. This case is expected to serve as a pilot initiative for waste PET recycling and foster profit creation for businesses in similar industries.

• The Korean Journal of Malacology
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• v.32 no.4
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• pp.263-268
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• 2016

Metallothionein (MT) family of metal-binding proteins are involved in maintaining homeostasis and heavy metal poisoning. Recently, MT has been considered as a biomarker that can identify a particular species, very similar to the use of cytochrome oxidase I (COI) gene. Satsuma myomphala species of land snails have been reported from North-East Asia, including South Korea and Japan. In particular, the land snail species have been known from only a limited area of Geoje Island, Gyeongsangnam-do province of South Korea. Genetic studies of S. myomphala has been limited with only 6 nucleotide, 2 protein registered on the NCBI server. For elucidating the genetic information of S. myomphala, we conducted RNA sequencing analysis using Illumina HiSeq 2500 next-generation platform. We screened the MT gene from the RNA-Seq database to confirm the molecular phylogenetic relationship. After sequencing, the de novo analysis and clustering generated 103,774 unigenes. After annotation against PANM database using BLAST program, we obtained MT sequence of 74 amino acid residues containing the coding region of 222 bp. Based on this sequence, we found about 53 sequences using the BLAST program in NCBI nr database. Using ClustalX alignment, Maximum-Likehood Tree of MEGA program, we confirmed the molecular phylogenetic relationships that showed similarity with mollusks such as Helix pomatia and H. aspersa, Megathura crenulata.

• Journal of Korea Water Resources Association
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• v.55 no.6
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• pp.437-446
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• 2022

In April 2020, the Korean government decided to operate the Hwacheon reservoir, a hydropower reservoir to supply water, and it is currently under pilot operation. Through the pilot operation, the Hwacheon reservoir is the first among the hydropower reservoirs in Korea to make a constant release for downstream water supply. In this study, the water supply capacity of the Hwacheon reservoir was estimated using the inflow data of the Hwacheon reservoir. A simulation model was developed to calculate the water supply that satisfies both the monthly water supply reliability of 95% and the annual water supply reliability of 95%. An optimization model was also developed to evaluate the water supply capacity of the Hwacheon reservoir. The inflow data used as input data for the model was modified in two ways in consideration of the impact of the Imnam reservoir. Calculating the water supply for the Hwacheon reservoir using the two modified inflows is as follows. The water supply that satisfies 95% of the monthly water supply reliability is 26.9 m³/sec and 24.1 m³/sec. And the water supply that satisfies 95% of the annual water supply reliability is 23.9 m³/sec and 22.2 m³/sec. Hwacheon reservoir has a maximum annual water supply of 777 MCM (Million Cubic Meter) without failure in the water supply. The Hwacheon reservoir can supply 704 MCM of water per year, considering the past monthly power generation and discharge patterns. If the Hwacheon reservoir performs a routine operation utilizing its water supply capacity, it can contribute to stabilizing the water supply during dry seasons in the Han River Basin.

• 한국유가공학회:학술대회논문집
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• 2007.09a
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• pp.49-58
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• 2007

Inflammatory process leads to the well-known mucosal damage and therefore a further disturbance of the epithelial barrier function, resulting abnormal intestinal wall function, even further accelerating the inflammatory process[1]. Despite of the records, etiology and pathogenesis of IBD remain rather unclear. There are many studies over the past couple of years have led to great advanced in understanding the inflammatory bowel disease(IBD) and their underlying pathophysiologic mechanisms. From the current understanding, it is likely that chronic inflammation in IBD is due to aggressive cellular immune responses including increased serum concentrations of different cytokines. Therefore, targeted molecules can be specifically eliminated in their expression directly on the transcriptional level. Interesting therapeutic trials are expected against adhesion molecules and pro-inflammatory cytokines such as TNF-${\alpha}$. The future development of immune therapies in IBD therefore holds great promises for better treatment modalities of IBD but will also open important new insights into a further understanding of inflammation pathophysiology. Treatment of cytokine inhibitors such as Immunex(Enbrel) and J&J/Centocor(Remicade) which are mouse-derived monoclonal antibodies have been shown in several studies to modulate the symptoms of patients, however, theses TNF inhibitors also have an adverse effect immune-related problems and also are costly and must be administered by injection. Because of the eventual development of unwanted side effects, these two products are used in only a select patient population. The present study was performed to elucidate the ability of TNF-${\alpha}$ antibodies produced in sheep colostrums to neutralize TNF-${\alpha}$ action in a cell-based bioassay and in a small animal model of intestinal inflammation. In vitro study, inhibitory effect of anti-TNF-${\alpha}$ antibody from the sheep was determined by cell bioassay. The antibody from the sheep at 1 in 10,000 dilution was able to completely inhibit TNF-${\alpha}$ activity in the cell bioassay. The antibodies from the same sheep, but different milkings, exhibited some variability in inhibition of TNF-${\alpha}$ activity, but were all greater than the control sample. In vivo study, the degree of inflammation was severe to experiment, despite of the initial pilot trial, main trial 1 was unable to figure out of any effect of antibody to reduce the impact of PAF and LPS. Main rat trial 2 resulted no significant symptoms like characteristic acute diarrhea and weight loss of colitis. This study suggested that colostrums from sheep immunized against TNF-${\alpha}$ significantly inhibited TNF-${\alpha}$ bioactivity in the cell based assay. And the higher than anticipated variability in the two animal models precluded assessment of the ability of antibody to prevent TNF-${\alpha}$ induced intestinal damage in the intact animal. Further study will require to find out an alternative animal model, which is more acceptable to test anti-TNF-${\alpha}$ IgA therapy for reducing the impact of inflammation on gut dysfunction. And subsequent pre-clinical and clinical testing also need generation of more antibody as current supplies are low.