• 한국식품영양과학회지
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• 제33권8호
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• pp.1343-1347
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• 2004

국내에서 시판 중인 요구르트의 CLA 함량과 상업용 dairy starter 유산균의 CLA 생산능을 조사하였다. 시판 요구르트 중의 CLA 함량은 4.1∼14.8 mg/100 g이었으며, 원료 우유사용비율이 낮아질수록 제품에 함유된 CLA 함량도 낮아지는 경향을 보였다. CLA 함량과 stearic acid, oleic acid, linoleic acid 및 linolenic acid의 함량, CLA 함량과 지방함량간에는 정의 상관관계를 보였다. 상업용 dairy starter 유산균 67균주 중 L. acidophilus 1종, L. casei 1종 및 Streptococcus thermophilus 3종 등 모두 5 균주가 기질로 첨가한 linoleic acid로부터 CLA를 생성하였다 또한 생성된 CLA는 cis-9, trans-11 이성체가 70% 이상으로 대부분을 차지하였다. 이들을 이용한 발효유는 대조구에 비해서 1.7∼2.6 mg/100 g의 CLA 함량의 증가를 보였으며, CLA 전환율은 6.1∼8.6%였다.

• Journal of Astronomy and Space Sciences
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• 제24권4호
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• pp.349-366
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• 2007

천체관측용 고효율 CCD 카메라 시스템과 제어소프트웨어의 독자개발의 시도로 KODAK사의 KAF-0401E($768\;{\times}\;512$), KAF-1602E($1536\;{\times}\;1024$), KAF-3200E($2184\;{\times}\;1472$) 등 세 종류의 CCD를 바꾸어 가며 장착할 수 있는 범용 CCD 카메라 시스템을 개발하였다. 기본적인 자료 입출력과 제어는 병렬 포트뿐만 아니라 입출력 속도가 상대적으로 바른 USB 포트로도 가능하도록 만들었다. ${\pm}18V$를 전원공급기로부터 공급받아 ${\pm}15V$와 +5V로 정류시켜서 시스템의 전원으로 사용하고 열전냉각소자 및 셔터는 ${\pm}12V$와 +5V로 동작되도록 하였다. 개발된 CCD 카메라는 전자회로기판을 2단으로 구성해서 공간 효율을 높였고, 전자회로의 구성은 컴퓨터에서 만들어진 클락 신호를 입력받아 CCD 동작을 위한 신호패턴을 만들어 주는 부분, CCD로부터 받은 신호를 증폭해서 디지털 신호로 바꿔주는 부분, 이 신호를 4비트씩 나누어서 병렬 포트에 전달하는 CCD 제어 부분과, 온도 센서의 신호를 디지털 신호로 바꾸어서 컴퓨터로 전달하는 온도제어 부분으로 이루어져 있다. 최대 냉각능력은 상온대비 ${\Delta}33^{\circ}C$이고, 이 온도 범위에서 약 $0.4^{\circ}C$의 정밀도로 제어 할 수 있다. 제작된 CCD 카메라 시스템의 읽기잡음은 $6e^-$이고, 이득은 $5e^-/ADU$이다. 모두 10대의 카메라가 만들어졌으며, CCD 카메라들은 작동시험에서 모두 만족한 결과를 얻었다.

• Journal of Ecology and Environment
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• 제44권3호
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• pp.126-142
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• 2020

Background: Most of the Pinus densiflora forests, occupying the largest area, have been restored in South Korea since the 1970s. As young pioneer forests, the succession process is under way. Since the forests are distributed nationwide and are vulnerable to disturbances, the process may differ depending on the geography and/or site conditions. Therefore, we reviewed the direction, the seral communities, and the late-successional species of progressive and disturbance-driven succession nationwide in the cool-temperate zone through meta-analysis and empirical observations. Main text: As a result of a meta-analysis of the direct succession and vertical structure, we found that the P. densiflora forest is in a directionally progressive succession, changing to the broadleaved forest after forming a mixed forest with its overwhelming successor, Quercus species (particularly Q. mongolica and Q. serrata). In dry stands in a relative sense, the Quercus species was favored occupying over 80% of the abundance of the succeeding species. Therefore, in dry stands, it is presumed that Quercus-dominated stage would last for a long time due to the current dominance and long life span, and eventually, it settles as Quercus-broadleaved forest with a site change. Contrary to this, it is presumed that in mesic stands where Quercus species do not occur or have low abundance, the late-successional broadleaved species settle early to form a co-dominant forest with multiple species. Due to geographical limits, the species composition of the two late-successional forests is different. Disturbances such as insect pests and fire retrogressed vulnerable P. densiflora forest for a while. However, it was mostly restored to the Quercus forest and is expected to be incorporated in the pathway of the dry stand. Conclusions: We revealed the succession process of P. densiflora forests according to geography and moisture and found that stand moisture had a decisive effect on the species and abundance of the successor. Although the P. densiflora forest is undergoing structural changes, the forest is still young; so within a few decades, physiognomy is not likely to change. Therefore, the decrease in the forest area may be due to other causes such as disturbances and forest conversion rather than due to succession.

• Asian-Australasian Journal of Animal Sciences
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• 제28권7호
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• pp.1006-1013
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• 2015

Heat-stress remains a costly issue for animal production, especially for poultry as they lack sweat glands, and alleviating heat-stress is necessary for ensuring animal production in hot environment. A high ${\gamma}$-aminobutyric acid (GABA)-producer Lactobacillus strain was used to investigate the effect of dietary GABA-producer on laying performance and egg quality in heat-stressed Hy-line brown hens. Hy-Line brown hens (n = 1,164) at 280 days of age were randomly divided into 4 groups based on the amount of freeze-dried GABA-producer added to the basal diet as follows: i) 0 mg/kg, ii) 25 mg/kg, iii) 50 mg/kg, and iv) 100 mg/kg. All hens were subjected to heat-stress treatment through maintaining the temperature and the relative humidity at $28.83{\pm}3.85^{\circ}C$ and 37% to 53.9%, respectively. During the experiment, laying rate, egg weight and feed intake of hens were recorded daily. At the 30th and 60th day after the start of the experiment, biochemical parameters, enzyme activity and immune activity in serum were measured. Egg production, average egg weight, average daily feed intake, feed conversion ratio and percentage of speckled egg, soft shell egg and misshaped egg were significantly improved (p<0.05) by the increasing supplementation of the dietary GABA-producer. Shape index, eggshell thickness, strength and weight were increased linearly with increasing GABA-producer supplementation. The level of calcium, phosphorus, glucose, total protein and albumin in serum of the hens fed GABA-producing strain supplemented diet was significantly higher (p<0.05) than that of the hens fed the basal diet, whereas cholesterol level was decreased. Compared with the basal diet, GABA-producer strain supplementation increased serum level of glutathione peroxidase (p = 0.009) and superoxide dismutase. In conclusion, GABA-producer played an important role in alleviating heat-stress, the isolated GABA-producer strain might be a potential natural and safe probiotic to use to improve laying performance and egg quality in heat-stressed hens.

• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.588-596
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• 2018

An endo-${\beta}$-1,4-glucanase gene, cel9K, was cloned using the shot-gun method from Paenibacillus sp. X4, which was isolated from alpine soil. The gene was 2,994 bp in length, encoding a protein of 997 amino acid residues with a predicted signal peptide composed of 32 amino acid residues. Cel9K was a multimodular enzyme, and the molecular mass and theoretical pI of the mature Cel9K were 103.5 kDa and 4.81, respectively. Cel9K contains the GGxxDAGD, PHHR, GAxxGG, YxDDI, and EVxxDYN motifs found in most glycoside hydrolase family 9 (GH9) members. The protein sequence showed the highest similarity (88%) with the cellulase of Bacillus sp. BP23 in comparison with the enzymes with reported properties. The enzyme was purified by chromatography using HiTrap Q, CHT-II, and HiTrap Butyl HP. Using SDS-PAGE/activity staining, the molecular mass of Cel9K was estimated to be 93 kDa, which is a truncated form produced by the proteolytic cleavage of its C-terminus. Cel9K was optimally active at pH 5.5 and $50^{\circ}C$ and showed a half-life of 59.2 min at $50^{\circ}C$. The CMCase activity was increased to more than 150% in the presence of 2 mM $Na^+$, $K^+$, and $Ba^{2+}$, but decreased significantly to less than 50% by $Mn^{2+}$ and $Co^{2+}$. The addition of Cel9K to a commercial enzyme set (Celluclast 1.5L + Novozym 188) increased the saccharification of the pretreated reed and rice straw powders by 30.4% and 15.9%, respectively. The results suggest that Cel9K can be used to enhance the enzymatic conversion of lignocellulosic biomass to reducing sugars as an additive.

• 대한약리학회지
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• 제10권1호
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• pp.55-59
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• 1974

Further elucidation of the mechanism of halothane's negative inotropic action has resulted from a study of the effect of various substrates on halothane-depressed rat atria. Approximately 6 mg% halothane was required to maintain a 50% depression of the contractility of rat atria suspended in a modified Krebs-Ringer bicarbonate glucose medium, pH 7.4, $30^{\circ}C$ for 2hr. Both lactate and acetate were found to restore partially the contractility of halothane-depressed atria. The maximally effective concentration of lactate was 5 mM; for acetate it was 2.5mM. Neither 5 nor 20 mM of additional glucose was effective in restoring the force of contraction of halothane-depressed atria. The results are consistent with the hypothesis that halothane exerts at least a part of its negative inotropic effect on rat atria by inhibiting either the uptake or utilization of glucose by the myocardium. The site of blockade must be prior to the conversion of pyruvate to acetyl CoA. In our previous report dealing with the mechanism of cardiac depressant action of inhalation anesthetic halothane, it has been demonstrated that: 1) approximately 6 mg/100 ml halothane is required to maintain 50% depression of the force of contraction of isolated rat atria in Krebs-Ringer bicarbonate glucose medium; 2) pyruvate partially restores the contractility of halothane-depressed atria, but has no effect on normal atria; the partial recovery of depressed atria by the addition of sodium pyruvate is due to the effect of the pyruvate ion itself, not to the sodium ion; 4) addition of pyruvate, to atria depressed with hypertonic medium, produced only further depression. From these findings we concluded that the cardiac depressant action of halothane on rat atria is a manifestation of inhibition of glucose uptake or utilization. The present studies were undertaken to observe the effect of other substrates on halothane-depressed atria in order to substantiate our conclusion. As with the case of pyruvate, lactate and acetate also partially restored the force of contraction of halothane-depressed atria. These data are consistent with the hypothesis that halothane inhibits glucose uptake or utilization in the glycolytic cycle of the myocardium.

• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.227-233
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• 2015

Two recombinant arabinosyl hydrolases, α-_L-arabinofuranosidase from Geobacillus sp. KCTC 3012 (GAFase) and endo-(1,5)-α-_L-arabinanase from Bacillus licheniformis DSM13 (BlABNase), were overexpressed in Escherichia coli, and their synergistic modes of action against sugar beet (branched) arabinan were investigated. Whereas GAFase hydrolyzed 35.9% of _L-arabinose residues from sugar beet (branched) arabinan, endo-action of BlABNase released only 0.5% of _L-arabinose owing to its extremely low accessibility towards branched arabinan. Interestingly, the simultaneous treatment of GAFase and BlABNase could liberate approximately 91.2% of _L-arabinose from arabinan, which was significantly higher than any single exo-enzyme treatment (35.9%) or even stepwise exo- after endo-enzyme treatment (75.5%). Based on their unique modes of action, both exo- and endo-arabinosyl hydrolases can work in concert to catalyze the hydrolysis of arabinan to _L-arabinose. At the early stage in arabinan degradation, exo-acting GAFase could remove the terminal arabinose branches to generate debranched arabinan, which could be successively hydrolyzed into arabinooligosaccharides via the endo-action of BlABNase. At the final stage, the simultaneous actions of exo- and endo-hydrolases could synergistically accelerate the _L-arabinose production with high conversion yield.

• 한국해양환경ㆍ에너지학회지
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• 제15권2호
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• pp.118-125
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• 2012

세계인구는 산업혁명 이후 급격히 증가하여 2012년 현재 약 70억 명이며, 2025년에는 80억 명까지 증가할 것으로 예측되고 있다. 개발도상국에서는 삶의 질이 개선됨에 따라 자원과 에너지의 수요가 전세계적으로 증가하고 있다. 결과적으로, 인류는 지구온난화, 물 부족, 식량부족, 자원 및 에너지 위기 등의 전지구적인 시급한 문제에 직면하고 있다. 이러한 문제점을 해결하기 위한 이산화탄소 해양격리, 해수담수화, 인공용승, 심해자원(개발), 해양에너지 등의 해양활용기술이 발전되고 있다. 이러한 기술들은 지속가능한 사회시스템보다는 화석연료에 기반한 편리함으로 인하여 발전되었기 때문에 지속가능성 및 공공의 수용 관점에서 이러한 기술들을 평가하는 것이 중요하다. 일본 조선해양공학회의 포괄적 해양압력 평가 및 분류기술 연구위원회는 지속가능성과 공공의 수용 입장에서 해양활용기술을 평가하기 위하여 환경 지속성과 경제적 타당성을 예측하는데 이용되는 지시자로써 트리플 I을 제안한 바 있다. 본 논문에서는 생태발자국과 환경영향평가가 결합된 트리플 I 지수, 트리플 I 지수의 개념과 구조, 트리플 I에서 경제-생태 전환 계수에 대하여 다루고 있다.

• 한국동물위생학회지
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• 제31권3호
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• pp.347-356
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• 2008

The purpose of this study was to evaluate the efficacy and cross-protection of serovar 12 against serovar 4 or 5 of H parasuis with M+$Parapac^{(R)}$. A total of 141 piglets from 2(A and B) farms were used and divided into experimental group and control group in each farm. Farm A has been detected H parasuis serovar 12, whereas farm B has been detected H parasuis serovar 4 or 5 from post-weaned pigs with PMWS. The piglets were vaccinated intramuscularly with 2.0ml of M+$Parapac^{(R)}$ in experimental group or normal saline in control group at 1 week of age. A same booster dose was given at 3 weeks of age. In order to value the antibody titer to H parasuis using by tube agglutination test, blood samples were collected from piglets at the aged of 1 week, 6 and 14 weeks. In experimental group and control group, the average antibody titers were $32.5{\pm}21.0,\;114.5{\pm}34.0,\;98.1{\pm}55.4$ and $32.9{\pm}18.6,\;25.8{\pm}36.9,\; 746.7{\pm}1,215.8$ at the aged of 1 week, 6 and 14 weeks, respectively. The cumulative clinical sign indexes by standard guideline of Schering-Plough Animal Health Corp were 486 and 1,069, respectively. The average daily gains and feed conversion rates were $0.553{\pm}0.016kg$ and $0.492{\pm}0.004kg$, and 1.99 and 2.24, respectively. The average gross lesion scores were $1.0{\pm}0.8$ and $1.9{\pm}0.6$, respectively. According to these results, the M+$Parapac^{(R)}$ containing H parasuis serovar 12 may be induce circulating antibodies that cross-react with serovar 4 or 5 and have a protection of PMWS with H parasuis.

• 한국지능시스템학회논문지
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• 제23권1호
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• pp.72-79
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• 2013

본 논문에서는 태양광 발전시스템의 최대전력점을 추종하기 위해, 개방회로전압과 가까운 첫 번째 지역극대전력점(local peak power point)의 전압 및 전류값이 특정한 범위 내에 있을 경우, 첫 번째 지역극대점이 전역극대전력점(global peak power point)인지 판단할 수 있도록 패턴을 분석하였다. 직-병렬 어레이로 연결된 태양전지 모듈에 부분그늘문제(partial shading problem)가 발생할 경우 다수의 지역극대전력점이 관찰될 수 있어, 전역극대전력점을 찾는데 어려움이 있다. 부분선형 태양전지 모델을 이용한 태블로 해석(Tableau analysis)으로 태양전지 어레이 회로의 V-I 특성을 시뮬레이션하여 지역극대전력점과 전역극대전력점을 확인하고, 그에 해당하는 전압 및 전류 값과 V-I 특성곡선의 패턴을 분석하였다. 분석된 패턴을 통해 특정한 영역을 설정하여 첫 번째 지역극대전력점이 전역극대전력점 인지 판단하여 발전하는 경우, 첫 번째 지역극대전력점으로만 발전했을 때에 비해 효율이 향상되었다.